Sławomir Borek

Sławomir Borek
Adam Mickiewicz University | UAM · Department of Plant Physiology

Ph.D., D.Sc.

About

46
Publications
13,111
Reads
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497
Citations
Introduction
By transcriptomic and proteomic approach I investigate a regulatory function of asparagine in the course of autophagy triggered by sugar starvation in embryonic axes of lupin germinating seeds. Particularly I am interested in the regulation of the degradation of autophagic bodies. I also look for the evidence on pexophagy in plant cells. The second topic of my research is the metabolism and signaling function of uncommon nucleotides in plants.
Additional affiliations
October 2019 - present
Adam Mickiewicz University, Poznań
Position
  • Professor (Associate)
September 2016 - December 2019
Adam Mickiewicz University
Position
  • Managing Director
October 2001 - September 2019
Adam Mickiewicz University
Position
  • Professor (Assistant)
Education
October 1996 - September 2001
Adam Mickiewicz University
Field of study
  • Plant physiology
October 1991 - July 1996
Adam Mickiewicz University
Field of study
  • Plant physiology

Publications

Publications (46)
Article
Full-text available
The research was conducted on embryo axes of yellow lupin (Lupinus luteus L.), white lupin (Lupinus albus L.) and Andean lupin (Lupinus mutabilis Sweet), which were isolated from imbibed seeds and cultured for 96h in vitro under different conditions of carbon and nitrogen nutrition. Isolated embryo axes were fed with 60mM sucrose or were sugar-star...
Article
Full-text available
Peroxisomes are cell organelles that play an important role in plants in many physiological and developmental processes. The plant peroxisomes harbor enzymes of the β-oxidation of fatty acids and the glyoxylate cycle; photorespiration; detoxification of reactive oxygen and nitrogen species; as well as biosynthesis of hormones and signal molecules....
Article
Full-text available
New players in plant signaling are described in detail in this review: extracellular ATP (eATP) and uncommon nucleotides such as dinucleoside polyphosphates (NpnN’s), adenosine 5′-phosphoramidate (NH2-pA), and extracellular NAD+ and NADP+ (eNAD(P)+). Recent molecular, physiological, and biochemical evidence implicating concurrently the signaling ro...
Article
Full-text available
Autophagy is an evolutionarily conserved process that occurs in yeast, plants, and animals. Despite many years of research, some aspects of autophagy are still not fully explained. This mostly concerns the final stages of autophagy, which have not received as much interest from the scientific community as the initial stages of this process. The fin...
Preprint
Full-text available
One of the processes that enable plants to respond efficiently to a changing environment, both internal and external, is autophagy. The efficient functioning of autophagy ensures proper growth and development of plants at every stage of ontogenesis. Under normal conditions, autophagy is a housekeeping process, allowing the recycling of damaged or u...
Article
Full-text available
It is known that cells contain various uncommon nucleotides such as dinucleoside polyphosphates (NpnN’s) and adenosine 5′-phosphoramidate (NH2-pA) belonging to nucleoside 5′‑phosphoramidates (NH2-pNs). Their cellular levels are enzymatically controlled. Some of them are accumulated in cells under stress, and therefore, they could act as signal mole...
Preprint
Full-text available
Special Issue in International Journal of Molecular Sciences, MDPI. One of the processes that enable plants to respond efficiently to a changing environment, both internal and external, is autophagy. The efficient functioning of autophagy ensures proper growth and development of plants at every stage of ontogenesis. Under normal conditions, autop...
Article
Full-text available
Autophagy, or self-eating, is a conservative process that occurs in yeast, animal, and plant cells, and primarily involves the degradation of cytoplasmic fragments along with organelles, protein complexes, and other macromolecules. In plants, autophagy participates in the circulation of cell components and acts as a quality control mechanism. Despi...
Article
It is known that the concentration of dinucleoside polyphosphates (NpnN's) in cells increases under stress and that adverse environmental factors induce biosynthesis of phenylpropanoids, which protect the plant against stress. Previously, we showed that purine NpnN's such as Ap3A and Ap4A induce both the activity of enzymes of the phenylpropanoid p...
Article
Full-text available
Polyhydroxyalkanoates (PHAs) make a large class of biodegradable biopolymers that are naturally synthesized by numerous microorganisms. These biopolymers could be an alternative to commonly used plastics based on petroleum. Production of PHAs in bioreactors using microorganisms is not widely applied due to its unprofitability. Using transgenic plan...
Article
Full-text available
Wysokie standardy rzetelności i skrupulatne przestrzeganie właściwego dla nauki systemu wartości muszą stanowić nieodłączny atrybut pracy naukowej, której główną inspiracją jest pogłębianie potwierdzonej wiedzy i poszerzanie jej horyzontu poza granice tego, co jest już znane, a także dzielenie się nią z innymi (Kodeks etyki pracownika naukowego, Ko...
Article
Full-text available
Bioart lub sztuka transgeniczna to nowa forma działań artystycznych, wykorzystująca techniki inżynierii genetycznej do transferowania naturalnego materiału genetycznego lub syntetycznych genów do organizmu w celu stworzenia nowej, unikalnej formy życia. Artyści tworzący dzieła bioartu, opierają swoją "twórczość" na wykorzystywaniu metod współczesne...
Article
Recent studies have shown that melatonin is an important molecule in plant physiology. It seems that the most important is that melatonin efficacy eliminates oxidative stress (direct and indirect antioxidant) and moreover induce plant stress reaction and switch on different defence strategies (preventively and interventively actions). In this repor...
Article
The research was conducted on yellow lupin (Lupinus luteus L.) mature seeds, developing cotyledons, developing pods, and seedlings. The main storage compound in yellow lupin seeds is protein, whose content may reach up to 45%. Oil content in seeds of yellow lupin is about 6%. In such protein-storing seeds there is a strong negative relationship bet...
Research
Full-text available
Plant Responses to Sugar Starvation. 2012. In: Carbohydrates - Comprehensive Studies on Glycobiology and Glycotechnology, Chuan-Fa Chang (Ed.), ISBN: 978-953-51-0864-1, InTech, Chapter 19:409- 438
Article
Full-text available
The main storage compound in lupin seeds is protein, whose content can reach up to 45–50 % of dry matter. However, seeds of some lupin species can also contain quite a large amount of storage lipid. The range of lipid content in lupin seeds is from about 6 to about 20 % of dry matter. Storage lipid in developing seeds is synthesized mainly from sug...
Article
Full-text available
Autophagy is a physiological process that occurs in yeast, animal and plant cells. During autophagy several cell components such as the cytoplasm, organelles or various types of protein complexes and other macromolecules are degraded. Autophagy occurs at a basal level under optimal conditions, but it is markedly enhanced as a result of various abio...
Chapter
Full-text available
Cyclic nucleotides, cAMP and cGMP, are important signaling molecules that control a range of cellular functions and modulate different reactions. The level of the cyclic nucleotides is elevated and decreased by nucleotide cyclases and phosphodiesterases, respectively. The adenylate and guanylate cyclases synthesize cAMP and cGMP from ATP and GTP, r...
Article
Full-text available
Regulation of starch accumulation in yellow (Lupinus luteus L.), white (L. albus L.), and Andean lupin (L. mutabilis Sweet) developing and germinating seeds was investigated. Research was conducted on cotyledons isolated from developing seeds as well as on organs of germinating seeds, that is, isolated embryo axes, excised cotyledons, and seedling...
Article
Full-text available
The regulatory function of sucrose in the activity of lipid-degrading enzymes was investigated in germinating seeds of yellow lupin (Lupinus luteus L.), white lupin (Lupinus albus L.) and Andean lupin (Lupinus mutabilis Sweet). The study was conducted on isolated embryo axes, excised cotyledons and seedlings cultured in vitro for 96 h on medium wit...
Data
The regulatory function of sucrose in the activity of lipid-degrading enzymes was investigated in germinating seeds of yellow lupin (Lupinus luteus L.), white lupin (Lupinus albus L.) and Andean lupin (Lupinus mutabilis Sweet). The study was conducted on isolated embryo axes, excised cotyledons and seedlings cultured in vitro for 96 h on medium wit...
Data
Full-text available
The regulatory function of sucrose in the activity of lipid-degrading enzymes was investigated in germinating seeds of yellow lupin (Lupinus luteus L.), white lupin (Lupinus albus L.) and Andean lupin (Lupinus mutabilis Sweet). The study was conducted on isolated embryo axes, excised cotyledons and seedlings cultured in vitro for 96 h on medium wit...
Article
Full-text available
Research of the regulatory function of sucrose in storage lipid breakdown was conducted on isolated embryo axes, excised cotyledons and whole seedlings of three lupine species grown in vitro on medium with 60 mM sucrose or without the sugar. Lack of sucrose in the medium caused significant increase in total lipid content in yellow, white and Andean...
Article
Full-text available
Research of the regulatory function of sucrose in storage protein breakdown was conducted on isolated embryo axes, excised cotyledons and whole seedlings of three lupine species grown in vitro on medium with 60 mM sucrose or without the sugar. Sucrose stimulated growth of yellow, white and Andean lupine isolated embryo axes and cotyledons but growt...
Article
Full-text available
Vegetable oils represent a very important, renewable source of human food, animal feed and have multiple industrial applications. Increasing demand from both food and non-food industry for vegetable oils and their components forces the search for new, more efficient and more economical sources of their acquisition. Today, it is not enough just to i...
Data
Full-text available
Research of the regulatory function of sucrose in storage protein breakdown was conducted on isolated embryo axes, excised cotyledons and whole seedlings of three lupine species grown in vitro on medium with 60 mM sucrose or without the sugar. Sucrose stimulated growth of yellow, white and Andean lupine isolated embryo axes and cotyledons but growt...
Data
Full-text available
Regulation by sucrose of storage compounds breakdown in germinating seeds of yellow lupine (Lupinus luteus L.), white lupine (Lupinus albus L.) and Andean lupine (Lupinus mutabilis Sweet): I. Abstract Research of the regulatory function of sucrose in storage protein breakdown was conducted on isolated embryo axes, excised cotyledons and whole seedl...
Article
Full-text available
Storage lipid and protein breakdown in germinating seeds of yellow (Lupinus luteus L.), white (L. albus L.), and Andean lupine (L. mutabilis Sweet) and regulatory function of sucrose were investigated. Less oil bodies were detected in organs of yellow lupine seeds, whereas the highest content of oil bodies was noticed in the Andean lupine seeds. Ma...
Data
Full-text available
Storage lipid and protein breakdown in germi-nating seeds of yellow (Lupinus luteus L.), white (L. albus L.), and Andean lupine (L. mutabilis Sweet) and regulatory function of sucrose were investigated. Less oil bodies were detected in organs of yellow lupine seeds, whereas the highest content of oil bodies was noticed in the Andean lupine seeds. M...
Article
The (14)C-acetate metabolism and regulatory functions of sucrose and sodium fluoride (NaF) were examined in embryo axes and cotyledons isolated from yellow lupine seeds and grown in vitro. After 15 min of incubating organs in solutions of labeled acetate, more radioactivity was found in amino acids (particularly in glutamate, asparagine and glutami...
Article
Full-text available
A comparative study was carried out on the dynamics of lipid accumulation in developing seeds of three lupine species. Lupine seeds differ in lipid content; yellow lupine (Lupinus luteus L.) seeds contain about 6%, white lupine (Lupinus albus L.) 7–14%, and Andean lupine (Lupinus mutabilis Sweet) about 20% of lipids by dry mass. Cotyledons from dev...
Article
The role of sucrose in the control of storage lipid mobilization was studied in germinating seeds of Lupinus luteus L. Concentrations of soluble sugars in isolated embryo axes, isolated cotyledons and in seedling axes and cotyledons cultured in vitro for 96 h on a medium without sucrose was maintained at a low level. One of the strategies of stabil...
Article
The metabolism of 14C-acetate was investigated during the in vitro germination of yellow lupine seeds. Carbon atoms (14C) from the C-2 position of acetate were incorporated mainly into amino acids: aspartate, glutamate, and glutamine and into sugars: glucose, sucrose, and fructose. In contrast to this, 14C from the C-1 position of acetate was relea...
Article
The intensity of protein reserve activation in yellow lupine (Lupinus luteus L.) organs cultured in vitro in the presence of saccharose and without sugar in the medium was studied. Isolated embryo axes, excised cotyledons and seeds deprived of their testae were grown on Heller medium: a) with 60 mM saccharose (+S), b) without sugar (−S) and c) for...
Article
The pathways of arginine transformations in organs of yellow lupin (Lupinus luteus L.) cultivated in vitro in the presence and absence of sucrose were investigated. Isolated embryo axes, isolated cotyledons and seeds deprived of their coat were cultured for 96 h on Heller medium with 60 mM saccharose (the fed variant, +S), without sugar (the starve...
Article
A study on globulins, major storage proteins in yellow lupin seeds, called conglutins, was conducted using SDS polyacrylamide gel electrophoresis. In this paper, an extensive and not yet published list of yellow lupin conglutins is presented. The patterns of subunits of major conglutins in seeds of three yellow lupin cultivars were similar to each...
Article
Isolated embryo axes from germinating seeds of Lupiruu angustifolius L. Emir cv. and Pisum sątivum L. Sol cv. were cultivated in vitro under various trophic conditions. The material was grown on Heller medium with 60 mmol dm-3 sucrose (the fed variant), and on Heller medium without sugar (the starved variant). Ultrathin sections used for observatio...
Article
Lemna minor L. was cultured for 48 h in aqueous solutions of leadnitrate (concentration of lead - 3 mg.dm-t) ut three pH values: 4.0, 5.7,7.5, which were chosen by lot. Lead toxicity increased with raising acidity' The inhibition of the growth rate of roots were: 66.7, 45.2 and 48.30%, respectively. One of the reasons of increased lead toxicity cou...

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Project
Special Issue in International Journal of Molecular Sciences, MDPI. One of the processes that enable plants to respond efficiently to a changing environment, both internal and external, is autophagy. The efficient functioning of autophagy ensures proper growth and development of plants at every stage of ontogenesis. Under normal conditions, autophagy is a housekeeping process, allowing the recycling of damaged or unnecessary organelles and protein complexes, and under various types of biotic and abiotic stresses can be an essential element of plant defense responses. The autophagic turnover of organelles and protein complexes occurs in a controlled and selective manner. The attention of many scientists is currently focused on identifying the elements of signaling pathways and the mechanisms of marking, recognizing, and directing particular cell components to autophagic degradation in the vacuole. This Special Issue will publish original research papers, reviews, short reviews, opinion articles, and hypotheses within the scope of the newest discoveries in signaling and autophagy in plants. In particular, we welcome papers showing molecular data on signal perception and transduction as well as selective types of autophagy in plants. https://www.mdpi.com/journal/ijms/special_issues/Signaling_Autophagy2
Project
We are currently organizing a Special Issue entitled “New Insight into Signaling and Autophagy in Plants” in the International Journal of Molecular Sciences (IJMS, ISSN 1422-0067; Impact Factor 4.556), an open access journal published by MDPI, Switzerland. https://www.mdpi.com/journal/ijms/special_issues/Signaling_Autophagy