Skander Elleuche

Skander Elleuche
EUROIMMUN Medical Laboratory Diagnostics AG | EUROIMMUN

Dr. rer. nat. habil.

About

71
Publications
11,919
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1,576
Citations
Citations since 2017
26 Research Items
928 Citations
2017201820192020202120222023050100150
2017201820192020202120222023050100150
2017201820192020202120222023050100150
2017201820192020202120222023050100150
Additional affiliations
January 2020 - present
EUROIMMUN Medical Laboratory Diagnostics AG
Position
  • Developer
October 2016 - December 2019
Miltenyi Biotec B.V. & Co. KG
Position
  • Team Coordinator
September 2009 - September 2016
Technische Universität Hamburg
Position
  • Lecturer
Education
April 2011 - April 2016
Technische Universität Hamburg
Field of study
  • Biotechnology/Molecular Biology
April 2006 - April 2009
Ruhr-Universität Bochum & Georg-August Universität Göttingen
Field of study
  • Microbiology/Genetics
October 2001 - March 2006
Ruhr-Universität Bochum
Field of study
  • Biology

Publications

Publications (71)
Presentation
Full-text available
This comic by authors Jan Friesen and Skander Elleuche together with illustrator Tyasseta and colorist Siloy explains the use of halophytes for bioenergy and is based on the review article: A. Debez*, I. Belghith*, J. Friesen*, C. Montzka*, S. Elleuche* (2017) Facing the challenge of sustainable bioenergy production: Could halophytes be part of the...
Book
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Ist Leben in heißer Säure oder in seifigen Flüssen vorstellbar? Wie tot ist das Tote Meer tatsächlich? Und welche Lebewesen frieren bereits bei 60 °C? Das Buch nimmt den Leser mit auf eine Reise zu den undenkbarsten ökologischen Nischen unseres Planeten und ihren Herrschern. In den faszinierendsten Habitaten geben mikroskopisch kleine Organismen de...
Article
Full-text available
Scientists are trained to tell stories, scientific stories. Training is also needed to comprehend and contextualize these highly nuanced and technical stories because they are designed to explicitly convey scientific results, delineate their limitations, and describe a reproducible “plot” so that any thorough reenactment can achieve a similar concl...
Article
Full-text available
Bacterial non-specific nucleases are ubiquitously distributed and involved in numerous intra- and extracellular processes. Although all nucleases share the basic chemistry for the hydrolysis of phosphodiester bonds in nucleic acid molecules, the catalysis comprises diverse modes of action, which offers great potential for versatile biotechnological...
Article
Full-text available
Can you imagine that there is an eight-legged bear that tolerates colder temperatures than the polar bears do in the Arctic? Can you imagine that this bear is able to grow older than the grizzly bears in North America? And can you imagine that this bear grows by molting, like spiders or snakes? These so-called water bears, scientifically named tard...
Article
Full-text available
Non-specific nuclease (NSN) can be applied in industrial downstream processing to remove nucleic acids from crude protein extracts or in cell-sorting systems to degrade nucleic acids derived from lysed cells. PsNuc from the ice-nucleating bacterium Pseudomonas syringae has the ability to decompose double- and single-stranded DNA in linear or circul...
Article
Full-text available
Non-specific nucleases (NSN) are of interest for biotechnological applications, including industrial downstream processing of crude protein extracts or cell-sorting approaches in microfabricated channels. Bacterial nucleases belonging to the superfamily of phospholipase D (PLD) are featured for their ability to catalyze the hydrolysis of nucleic ac...
Article
Full-text available
Bacterial non-specific nucleases of the phospholipase D family are widely distributed among the members of the Enterobacteriaceae. Each genome mainly contains a single copy of a gene encoding a phospholipase D family protein. However, two distantly related isozymes (< 40% identity at the protein level) were identified by BLAST-analyses in the plant...
Article
Dem 1907 im ehemaligen Palästina geborenen Benjamin Elazari-Volcani glückte 1936 der Nachweis halobionter Mikroorganismen in dem extrem salzhaltigen Toten Meer. Damit bewies er, dass dieses keineswegs tot ist und legte den Grundstein zur Erforschung sogenannter extremophiler Mikroorganismen. Viel später, als sich Elazari-Volcani nach einer ersten K...
Article
Full-text available
Objectives Metal-ion independent non-specific nucleases are of high potential for applications in EDTA-containing bioprocessing workflows. Results A novel extracellular non-specific nuclease EcNuc from the enterobacterium Escherichia coli has been identified. The recombinant gene was expressed and the protein was purified. Maximum activity of the...
Chapter
Außergewöhnliche Orte auf unserer Erde zeichnen sich durch eindrucksvolle Bewohner und ihre cleveren Anpassungsstrategien an einzigartige Lebensbedingungen aus. Durch seine behaarten Sohlen fühlt sich der Wüstenwuchs selbst auf dem heißen Boden der Wüste sehr wohl. Ebenso kennt wahrscheinlich jeder die Bilder des verschlafenen Eisbären, der sich na...
Chapter
Wenden wir uns nun den basischsten und sauersten Lebensräumen auf unserem Planeten zu. Auch hier finden wir eine dichte Besiedelung mit extremophilen Mikroorganismen. Sie nutzen die pH-Skala von 0 bis 14 beinahe komplett aus, während Tiere und Pflanzen in den sauersten und basischsten Habitaten fast vollständig fehlen.
Chapter
Am Ende begeben wir uns zurück an den Anfang. Damit ist allerdings nicht der Anfang des Buches gemeint, sondern der Moment, als die erste lebende Zelle entstanden ist. An dieser Stelle werden wir die Astrobiologie wieder verlassen, da wir vermutlich auf der Erde bleiben können, um unserem allerersten gemeinsamen Vorfahren zu begegnen.
Chapter
Durch den Menschen verursachte Katastrophen führen zu nachhaltigen Veränderungen in der Natur. Deutlich wurden menschliche Eingriffe durch einen rücksichtslosen Umgang mit unseren natürlichen Ressourcen sowie in jüngster Vergangenheit durch Unfälle bei der Erdölförderung, durch die industrielle Vergiftung von Flüssen und Böden und durch Nuklearkata...
Chapter
Die Sehnsucht nach spektakulären wissenschaftlichen Entdeckungen ist so alt wie die Wissenschaften selbst. Einige der größten Errungenschaften prägen sich im öffentlichen Gedächtnis nachhaltig ein und verblassen praktisch nie. Oft sind mit diesen Entdeckungen ihre Entdecker untrennbar verbunden, oder aber die Erstentdeckung gerät in Vergessenheit,...
Chapter
Seit Jahrtausenden nutzt die Menschheit die Biokatalyse zur Herstellung von Wein, Bier und Brot. Aber erst durch Forschungsarbeiten der vergangenen 200 Jahre beginnen wir, Zellen und Enzyme als Biokatalysatoren tatsächlich zu verstehen. Heutzutage besteht die Möglichkeit, klassische Prozesse der organischen Synthese umweltfreundlicher zu gestalten...
Chapter
Menschen bevorzugen gemäßigte Temperaturen, halten sich gerne im Trockenen auf (nur bitte nicht zu trocken), brauchen Luft zum Atmen und ausreichend Nahrung. Einige Mikroben frieren dagegen nicht so schnell, anderen kann es nicht heiß genug sein und wieder andere fühlen sich in saurer Umgebung oder in extrem salzigen Biotopen am wohlsten. Zum Glück...
Chapter
Die Faszination des Menschen für extreme Biotope beschränkt sich nicht nur auf seinen eigenen Planeten. Die Begeisterung richtet sich auch über die Grenzen der Erde hinaus auf das Weltall. Die große Sehnsucht, andere Planeten wie den uns nahen Mars zu erkunden und vielleicht eines Tages zu besiedeln, ist längst nicht mehr nur Bestandteil von Scienc...
Chapter
Ein gängiger Tipp für die Reise in ferne und exotische Länder ist oft, das Trinkwasser abzukochen oder das Essen gut durchzubraten, um mögliche Keime abzutöten. Bei manchen Einzellern ist dieser Hinweis jedoch wertlos, da sie sich erst im Sand der Wüste, im kochenden Wasser der farbigsten Thermalquellen oder im Erdinneren so richtig wohl fühlen. Di...
Chapter
Der größte Teil unseres Planeten ist zu kalt für den Menschen. Wir würden in den Tiefen der Ozeane, im Eis und Schnee der Polargebiete und auch in den trockenen Gebieten der arktischen Tundra und der antarktischen Oasen erfrieren. Kälteliebende Mikroben sind in diesen Regionen viel ausdauernder und fühlen sich erst in eisiger Kälte besonders wohl.
Chapter
Fast das gesamte Wasser auf der Erde ist zu salzhaltig, um es trinken zu können. Wenn ein Schiffbrüchiger Salzwasser trinkt, kann er seinen Durst nicht stillen. Der menschliche Körper würde nämlich Wasser aus den Zellen freigeben, um das aufgenommene Salz in verdünnter Form wieder ausscheiden zu können.
Chapter
Handelt es sich bei der Tiefsee um einen extremen Lebensraum? Für den Menschen ist es viel zu unkomfortabel dort unten. Es ist kalt oder extrem heiß. Es ist dunkel. Es gibt meist nur wenig Sauerstoff und kaum Nahrungsmittel.
Chapter
Inteins are internal in-frame insertions transcribed and translated together with their host protein and excised at the protein level. The posttranslational process that excises the intein from a precursor protein with subsequent ligation of the flanking protein fragments, called N- and C-exteins, is termed protein splicing. Inteins have predominan...
Article
Full-text available
Due to steadily growing population and economic transitions in the more populous countries, renewable sources of energy are needed more than ever. Plant biomass as a raw source of bioenergy and biofuel products may meet the demand for sustainable energy; however, such plants typically compete with food crops, which should not be wasted for producin...
Article
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Do you like to stay at the beach on a hot summer day? Sun bathing, chilling, and playing beach games? If it gets too hot, you can quickly cool off in the lukewarm ocean. Can you believe that there are living organisms on our planet that would still freeze on the hottest day of the year? These tiny creatures are called heat-loving microbes, and they...
Article
Full-text available
The cold-adapted pullulanase Pul13A is an industrial useful amylolytic enzyme, but its low solubility is the major bottleneck to produce the protein in recombinant form. In a previous approach, a complex and time-consuming purification strategy including a step-wise dialysis procedure using decreasing concentrations of urea to renature the insolubl...
Article
Full-text available
The supply of industrially relevant biocatalysts demands an easy and efficient protein production in high yield. In a conventional approach, a recombinant protein is produced in a heterologous host enabling the manipulation of multiple parameters including expression plasmids, growth conditions and regulation of protein biosynthesis. In this study,...
Article
Full-text available
Thermus brockianus strain GE-1 is a thermophilic, Gram-negative, rod-shaped and non-motile bacterium that was isolated from the Geysir geothermal area, Iceland. Like other thermophiles, Thermus species are often used as model organisms to understand the mechanism of action of extremozymes, especially focusing on their heat-activity and thermostabil...
Article
Full-text available
The “8th International Congress on Biocatalysis (biocat2016)” is part of a biennial conference series. Biocatalysis is a topic based on the edge of biology and chemistry, which brings together scientists from the life sciences, engineers and computer scientists. This international conference serves as a platform to meet researchers from all over th...
Chapter
The continuous need for innovative sustainable technologies has led to an increasing interest in biocatalysts that can be applied in various industrial sectors including chemical, pharmaceutical, textile, paper and pulp, food and feed industries as well as for the production of renewable energy. In order to develop a new generation of efficient bio...
Chapter
Lipolytic enzymes include esterases and lipases that are capable of hydrolyzing and synthesizing ester linkages in triglycerides. These ubiquitous biocatalysts are found in all domains of life. However, the focus of this chapter exclusively covers lipolytic extremozymes from psychro- and (hyper-)thermophiles, while enzymes from mesophilic prokaryot...
Chapter
Inteins are internal protein elements that auto-catalyze their excision from a precursor protein and the ligation of the flanking sequences (exteins) with a peptide bond. This post-translational process is called cis protein splicing and involves only four intramolecular reactions, and a small number of key catalytic residues in the intein and exte...
Article
Full-text available
Plant cell walls are composed of complex polysaccharides such as cellulose and hemicellulose. In order to efficiently hydrolyze cellulose, the synergistic action of several cellulases is required. Some anaerobic cellulolytic bacteria form multienzyme complexes, namely cellulosomes, while other microorganisms produce a portfolio of diverse enzymes t...
Article
Full-text available
Degradation of complex plant biomass demands a fine-regulated portfolio of glycoside hydrolases. The LE (LguI/Eco81I)-cloning approach was used to produce two enzyme chimeras CB and BC composed of an endoglucanase Cel5A (C) from the extreme thermophilic bacterium Fervidobacterium gondwanense and an archaeal beta-glucosidase Bgl1 (B) derived from a...
Chapter
The discovery of extremophilic microorganisms (extremophiles) and their enzyme systems (extremozymes) has opened new opportunities for various industrial applications over the last decades. Depending on their specific properties such as high thermostability, tolerance at extreme pH values or their performance at high organic solvent concentrations,...
Article
Industrial processes often take place under harsh conditions that are hostile to microorganisms and their biocatalysts. Microorganisms surviving at temperatures above 60°C represent a chest of biotechnological treasures for high-temperature bioprocesses by producing a large portfolio of biocatalysts (thermozymes). Due to the unique requirements to...
Article
Extein amino acid residues around the splice site junctions affect the functionality of inteins. To identify an optimal sequence context for efficient protein splicing of an intein from the thermoacidophilic archaeon Picrophilus torridus, single extein amino acid residues at the splice site junctions were continuously deleted. The construction of a...
Article
An activity-based screening approach led to the identification of a novel glycoside hydrolase family-13 pullulanase gene (pul13A) from a psychrophilic bacterium. The recombinant enzyme exhibited a deduced peptide sequence of 1,440 amino acid residues and was produced in a heterologous host in Escherichia coli. Purification from inclusion bodies was...
Article
It is a mammoth task to develop a modular protein toolbox enabling the production of posttranslational organized multifunctional enzymes that catalyze reactions in complex pathways. However, nature has always guided scientists to mimic evolutionary inventions in the laboratory and, nowadays, versatile methods have been established to experimentally...
Article
The LE (LguI/Eco81I)-cloning procedure described here allows a step-wise, directional fusion of multiple DNA-fragments into a vector system by utilizing two restriction enzymes generating identical non-palindromic overhangs. This strategy was applied to produce heat-stable cellulase-fusion proteins containing up to five single moieties. Terminal af...
Article
Bifunctional enzyme constructs were generated comprising two genes encoding heat-active endoglucanase (cel5A) and endoxylanase (xylT). The fused proteins Cel5A-XylT and XylT-Cel5A were active on both β-glucan and beechwood xylan. An improvement in endoglucanase and endoxylanase catalytic activities was observed. The specific activity of the fusion...
Article
There is a considerable potential of cold-active biocatalysts for versatile industrial applications. A psychrophilic bacterial strain, Shewanella arctica 40-3, has been isolated from arctic sea ice and was shown to exhibit pullulan-degrading activity. Purification of a monomeric, 150-kDa pullulanase was achieved using a five-step purification appro...
Article
Extremozymes are enzymes derived from extremophilic microorganisms that are able to withstand harsh conditions in industrial processes that were long thought to be destructive to proteins. Heat-stable and solvent-tolerant biocatalysts are valuable tools for processes in which for example hardly decomposable polymers need to be liquefied and degrade...
Article
Thermostable enzymes are required for application in a wide range of harsh industrial processes. High stability and activity at elevated temperatures, as well as high tolerances towards various reagents and solvents, are needed. In this work, a glycoside hydrolase family 1 β-glucosidase (Bgl1) of archaeal origin was isolated from a hydrothermal spr...
Article
The rapid interconversion of carbon dioxide and bicarbonate (hydrogen carbonate) is catalyzed by metalloenzymes termed carbonic anhydrases (CAs). CAs have been identified in all three domains of life and can be divided into five evolutionarily unrelated classes (α, β, γ, δ and ζ) that do not share significant sequence similarities. The function of...
Article
Unlabelled: Carbonic anhydrases (CAs) are metalloenzymes catalyzing the reversible hydration of carbon dioxide to bicarbonate (hydrogen carbonate) and protons. CAs have been identified in archaea, bacteria and eukaryotes and can be classified into five groups (α, β, γ, δ, ζ) that are unrelated in sequence and structure. The fungal β-class has only...
Article
NAD(P)(+)-dependent alcohol dehydrogenases (ADH) are widely distributed in all phyla. These proteins can be assigned to three nonhomologous groups of isozymes, with group III being highly diverse with regards to catalytic activity and primary structure. Members of group III ADHs share a conserved stretch of amino acid residues important for cofacto...
Article
Full-text available
Living organisms have developed strategies to cope with alcoholic substrates. Due to the manifold appearance of alcoholic compounds, enzymes that are capable of decomposing or processing such molecules have emerged several times during evolution. These proteins are the so-called alcohol dehydrogenases, a group of ubiquitous enzymes that catalyse th...
Chapter
Starch represents a ubiquitous molecule in plants and is composed of linear polymer amylose and branched polymer amylopectin. Due to its complex structure, it is insoluble in water and needs to be liquefied at high temperatures to make it a useable substrate for hydrolyzing biocatalysts. Hyper-/thermophilic microorganisms belonging to Archaea and B...
Article
Alcohol dehydrogenases are highly diverse enzymes catalysing the interconversion of alcohols and aldehydes or ketones. Due to their versatile specificities, these biocatalysts are of great interest for industrial applications. The adh3-gene encoding a group III alcohol dehydrogenase was isolated from the gram-positive bacterium Oenococcus oeni and...
Article
Purpose of work A pair of NAD+- and NADP+-dependent group III-alcohol dehydrogenases was characterized from the enterobacterium, Dickeya zeae, to expand our understanding of the distribution and biochemical properties of this interesting group of enzymes. Two putative group III-alcohol dehydrogenases (ADHs) were identified in the genome of Dickey...
Article
The reduction of fossil fuels, coupled with its increase in price, has made the search for alternative energy resources more plausible. One of the topics gaining fast interest is the utilization of lignocellulose, the main component of plants. Its primary constituents, cellulose and hemicellulose, can be degraded by a series of enzymes present in m...
Article
ten beschrieben wurden. Ein bekanntes eukaryoti- sches Beispiel ist der salzliebende (halophile) Salinen- krebs (Artemia salina), der seit mehr als 200 Millio- nen Jahren die Salzseen der Erde bevölkert. Organis- men, die dagegen in ökologischen Nischen mit besonders hohen Temperaturen (bis 80 °C) leben, wer- den als Thermophile bezeichnet. A...
Chapter
The gene family of carbonic anhydrases consists of enzymes that are capable to rapidly accelerate the spontaneous and reversible interconversion from carbon dioxide to bicarbonate. To date, fungal carbonic anhydrases have been identified in the genomes of some ‘basal fungi’, ascomycetes, basidiomycetes and also within the so-called group of non-fun...
Article
The OsmC-region (osmotically induced protein family) of the two-domain esterase EstO from the psychrotolerant bacterium Pseudoalteromonas arctica has been shown to increase thermolability. In an attempt to test if these properties can be conferred to another enzyme, we genetically fused osmC to the 3'-region of the family 8 xylanase encoding gene x...
Article
In fungi, the homoeodomain protein STE12 controls diverse developmental processes, and derives its regulatory specificity from different protein interactions. We recently showed that in the homothallic ascomycete Sordaria macrospora, STE12 is essential for ascospore development, and is able to interact with the alpha-domain mating-type protein SMTA...
Article
Full-text available
Inteins are internal protein elements that self-excise from their host protein and catalyze ligation of the flanking sequences (exteins) with a peptide bond. They are found in organisms in all three domains of life, and in viral proteins. Intein excision is a posttranslational process that does not require auxiliary enzymes or cofactors. This self-...
Article
A gene encoding an esterase (estO) was identified and sequenced from a gene library screen of the psychrotolerant bacterium Pseudoalteromonas arctica. Analysis of the 1,203 bp coding region revealed that the deduced peptide sequence is composed of 400 amino acids with a predicted molecular mass of 44.1 kDa. EstO contains a N-terminal esterase domai...
Article
Full-text available
Carbonic anhydrases (CAs) are metalloenzymes that catalyse the interconversion of carbon dioxide and bicarbonate with high efficiency. This reaction is fundamental to biological processes such as respiration, photosynthesis, pH homeostasis, CO(2) transport and electrolyte secretion. CAs are distributed among all three domains of life, and are curre...
Chapter
Inteins are internal protein domains found inside the coding region of different proteins. They are transcribed and translated together with their host protein and are removed from the unprocessed protein by an autocatalytic self-excision process, termed protein splicing. Large inteins comprise independent protein-splicing and endonuclease domains...
Article
Full-text available
The ubiquitous metalloenzyme carbonic anhydrase (CA) catalyzes the interconversion of carbon dioxide and bicarbonate. This enzyme has been investigated in mammals, plants, algae, bacteria, archaea and fungi. Based on distinct structural characteristics, CAs can be assigned to five independently evolved classes (alpha, beta, gamma, delta and zeta)....
Article
Inteins are protein-intervening sequences that are translated with the host protein and can self-excise themselves post-translationally in an autocatalytic process. The flanking regions--called exteins--are then re-ligated with a new peptide bond, resulting in a mature host protein. Previously, we have identified inteins in the highly conserved 3.2...
Article
Full-text available
Carbon dioxide (CO2) is among the most important gases for all organisms. Its reversible interconversion to bicarbonate (HCO3−) reaches equilibrium spontaneously, but slowly, and can be accelerated by a ubiquitous group of enzymes called carbonic anhydrases (CAs). These enzymes are grouped by their distinct structural features into α-, β-, γ-, δ- a...
Article
Full-text available
We report the utilization of Discosoma sp. red fluorescent protein DsRed to visualize peroxisomes in the filamentous ascomycete Sordaria macrospora. To achieve labeling of peroxisomes, DsRed was fused to a serine-lysine-leucine tag (SKL). Expression of the DsRed-SKL fusion gene under the control of the Aspergillus nidulans gpd-promoter led to prote...
Article
Cyanase degrades toxic cyanate to NH3 and CO2 in a bicarbonate-dependent reaction. High concentrations of cyanate are fairly toxic to organisms. Here, we characterize a eukaryotic cyanase for the first time. We have isolated the cyn1 gene encoding a cyanase from the filamentous ascomycete Sordaria macrospora and functionally characterized the cyn1...
Article
Inteins are internal protein splicing elements that can autocatalytically self-excise from their host protein and ligate the protein flanks (exteins) with a peptide bond. Large inteins comprise independent protein splicing and endonuclease domains whereas mini-inteins lack the central endonuclease domain. To identify mini-intein domains that are es...
Article
Amidase 1 (AMI1), a specific indole-3-acetamide amidohydrolase, is an Arabidopsis thaliana amidase signature enzyme that catalyzes the synthesis of indole-3-acetic acid from indole-3-acetamide. Amidase signature family members catalyze a diverse range of enzymatic reactions and are found widespread in nature, for instance in bacteria, mammals, and...
Article
Inteins are internal protein domains found inside the coding region of different proteins. They can autocatalytically self-excise from their host protein and ligate the protein flanks, called exteins, with a peptide bond via a post-translational process called protein cis-splicing. In contrast, protein trans-splicing involves inteins split into an...
Article
Full-text available
Inteins are protein-intervening sequences found inside the coding region of different host proteins and are translated in-frame with them. They can self-excise through protein splicing, which ligates the host protein flanks with a peptide bond. In this study, four different species of the genus Penicillium were investigated for the presence of inte...
Article
Ein Intein ist eine Sequenz, die sich posttranslational und autokatalytisch aus einem Vorläuferprotein herausgespleißt. Inteine kommen in allen drei Bereichen des Lebens (Prokaryoten, Eukaryoten, Archaea) vor und werden auch als “Proteinintrons” bezeichnet. Im Gegensatz zu Introns, bei denen die Spleißreaktion auf RNA-Ebene stattfindet, schneiden s...

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