Sjanna Besteman- Doctor of Medicine
- pediatrician at Academisch Medisch Centrum Universiteit van Amsterdam
Sjanna Besteman
- Doctor of Medicine
- pediatrician at Academisch Medisch Centrum Universiteit van Amsterdam
Pediatrician at Amsterdam UMC
About
10
Publications
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Introduction
Skills and Expertise
Current institution
Publications
Publications (10)
Respiratory syncytial virus (RSV) is the most prevalent cause of acute lower respiratory infection in young children. Currently, the first RSV vaccines are approved by the FDA. Recently, N6-methyladenosine (m⁶A) RNA methylation has been implicated in the regulation of the viral life cycle and replication of many viruses, including RSV. m⁶A methylat...
Background
Respiratory syncytial virus (RSV) infection is the primary cause of lower respiratory tract infections in children under 5 years of age. Monocytes, especially in the respiratory tract are suggested to contribute to RSV pathology but their role is incompletely understood. With transcriptomic profiling of blood and airway monocytes, we des...
Signal inhibitory receptor on leukocytes-1 (SIRL-1) is an immune inhibitory receptor expressed on human granulocytes and monocytes that dampens antimicrobial functions. We previously showed that sputum neutrophils from infants with severe respiratory syncytial virus (RSV) bronchiolitis have decreased SIRL-1 surface expression compared with blood ne...
Background
Recurrent respiratory syncytial virus (RSV) infection requiring hospitalization is rare and the underlying mechanism is unknown. We aimed to determine the role of CD14-mediated immunity in the pathogenesis of recurrent RSV infection.
Methods
We performed genotyping and longitudinal immunophenotyping of the first patient with a genetic C...
Signal inhibitory receptor on leukocytes-1 (SIRL-1) is an immune inhibitory receptor expressed on human granulocytes and monocytes which dampens antimicrobial functions. We previously showed that sputum neutrophils from infants with severe respiratory syncytial virus (RSV) bronchiolitis have decreased SIRL-1 surface expression compared to blood neu...
Respiratory syncytial virus (RSV) is an important infectious agent in infants and young children. In most cases, RSV infection only causes mild disease, but in some, it requires invasive ventilation. Although antiviral drugs are obvious candidates to treat viral illness, and some have shown antiviral effects in humans, antivirals such as GS‐5806, A...
Background:
Neutrophils are the most abundant cell type infiltrating the airways during severe respiratory syncytial virus (RSV) infection. Their exact role in disease pathophysiology remains enigmatic. Therefore, we determined genome-wide RNA expression profiles of local and systemic neutrophils in RSV bronchiolitis to provide further insight int...
During severe respiratory syncytial virus (RSV) bronchiolitis there is a massive influx of activated neutrophils to the lungs. An exaggerated immune response contributes to lung damage and disease severity during RSV infection. We have previously shown that normal adult neutrophil function can be modulated by agonists of SIRL-1. Here we aimed to me...
Questions
Question (1)
I co-cultured neutrophils with differentiated epithelial cells in different conditions (infected and non-infected cultures). I want to measure neutrophil induced epithelial damage. I still have al lot of cell lysates (after neutrophil incubation, neutrophils were washed of the cells an then cells were lysed). Does anyone no which genese or proteins are best to test to measure epithelial apoptosis in this situation? Are there for instance any genes specific for epithelial cells, so apoptotic neutrophils that might have penetrated in the cultures don't influence the results? Or is there a way to correct for possible neutrophils in the lysate? Thank you.
