Sivaramakrishnan Ramachandiran

Apollo Healthcare lifestyle Limited · Department of Molecular Biology

The enzyme hyaluronidase cleaves the substrate hyaluronic acid. In the study, hyaluronidase was isolated from the venom gland of C. betulinus and characterised using SDS-PAGE, FTIR, and HPLC. The protein content of crude venom is approximately 4mg/ml, whereas purification with Sepacryl S-100 yielded 0.04mg/ml protein with 0.463TRU/mg specific activ...

In recent decades, marine organisms are highly investigated by several scientific communities due to their potential biological properties includes antioxidant, anti-inflammatory, and anti-microbial activities. Among the marine species, brown seaweeds have a crucial role in the construction of diverse bioactive compounds. The present study was main...

Real time PCR (RT-PCR) detection method is the widely used for COVID-19 virus detection. This includes sample collection in viral transport medium (VTM), viral RNA extraction followed by detection of virus using fluorescence dye-based system using RT-PCR machine. Several studies have demonstrated a new method which replaces the extraction step by a...

Tyrosinase is a ubiquitous enzyme found in most pigmented animals. In the present study, the tyrosinase enzyme was isolated and purified from Sepiella inermis ink and its application in phenolic compounds removal from aqueous solution. The presence of the tyrosinase in the S. inermis ink was read at 280nm and the crude enzyme was purified by dialys...

Stingrays envenomation in humanswere the common accident in the marine and freshwater ecosystem. To determine such effect species Himantura imbricata have been used to elucidate Hemolytic activity, Plasma Coagulation, Fibrin coagulation, Fibrinolytic activity, Activated Partial Thromboplastin Time (APTT),and Prothrombin Time (PT) effects were studi...

Present study demonstrated the isolation of promising β-galactosidase producing bacterial strain from mangrove soil. 16s rRNA sequence analysis identified the bacterial strains as Steptococcusthermophillus and Escherichia coli. Upon growth studies S. thermophilus and E. coli entered the logarithmic phase at around 48 and 36 hours of incubation with...

Phenylalanine, aromatic amino acid and clupadonic acid, a long-chain fatty acid were purified from methanol and methanol-dichloromethane extracts of marine sponge H. erecta Keller. It was characterized by using UHPLC-Triple quadrupole ESI/MS. The clupadonic acid isolated possessed good inhibition towards α-amylase and α-glucosidase with IC50 of 17μ...

A marine sea-grass Cymodocaea serrulata was collected, dried and extracted with a different solvent. The presences of phytochemical constituents were confirmed and the ethyl acetate extraction was considered to be a suitable solvent. In addition, the crude seagrass was serially diluted and spread onto the Zobell Marine Agar plates. Among the eight...

The present study was carried out to evaluate the anti-cancer properties of protein hydrolysate from posterior salivary gland (PSG) of Amphioctopus membranaceus. The sample (octopus) was initially screened for species identification through CO-I gene. The PSG of the animal was homogenized and centrifuged at 6000 rpm for 10 min. Further, enzymatic h...

The antioxidant and antibacterial potential of various solvent extracts (water, 100% and 80% methanol) of Perna viridis was evaluated. The tissue analyzed for the macronutrient composition was estimated as the moisture (84%), proteins (63.5%), carbohydrates (24.26%) and lipids (9.99%). The crude extracts subjected to 10% SDS-PAGE analysis showed an...

The sulfated polysaccharide fucoidan was extracted from brown algae Turbinaria conoides using hot water and purified by anion‐exchange chromatography. The biochemical and monosaccharide composition of purified fucoidan was studied by colorimetric assays and Gas Chromatography Mass Spectrometry (GCMS). The purity of fucoidan was confirmed by agarose...

Sodium Alginate (SA) is an excellent carrier in various drug delivery systems. In this study, SA was synthesized from brown seaweed, Turbinaria conoides with a yield of 31.3 AE 0.86%. The analysis of physicochemical properties of extracted alginate (ALG) determined its purity. The structural confirmations of ALG were studied through FTIR, XRD and S...

The present study investigated the anticancer and antimicrobial properties of ink gland from Kalinga ornata using three different solvents such as acetone (AIG), ethyl acetate (EIG) and methanol (MIG). The ink gland sample was extracted with three different solvents and subjected to anti-microbial activities and then purified through CM-Sephadex, t...

A growing impetus in the field of polysaccharide research has led to the discovery of novel drugs for various diseases. The echinoid shells contain a large proportion of minerals, especially Calcium and fewer amounts of polysaccharides, proteins, method. The physicochemical characterization of the crude polysaccharide from Salmacis virgulata, S. bi...