Siti Isrina Oktavia Salasia

• Universitas Gadjah Mada

Background and Aim: Staphylococcus aureus is a significant foodborne pathogen responsible for producing enterotoxins, particularly staphylococcal enterotoxins A (SEA) and staphylococcal enterotoxins B (SEB), which are frequently found in milk and dairy products. These toxins in raw milk pose a risk to public health, necessitating accurate and rapid...

BACKGROUND: Ultraviolet B (UVB) radiation is commonly known to be related to skin inflammation. The inflammation process is orchestrated by many cell types, including immune cells. Changes in bone marrow cellularity can also be an indicator of inflammation. Megakaryocytes, myeloid immune cells progenitor and erythroid progenitor cells, are at high...

During surveillance of Staphylococcus aureus in lesions from patients with atopic dermatitis (AD), we isolated Staphylococcus argenteus, a species registered in 2011 as a new member of the genus Staphylococcus and previously considered a lineage of S. aureus. Genome sequence comparisons between S. argenteus isolates and representative S. aureus cli...

Background and Aim The high prevalence of diabetes mellitus in Indonesia indirectly reflects the high risk of developing chronic wounds that are susceptible to infection. Methicillin-resistant Staphylococcus aureus (MRSA) is an infectious pathogen that is resistant to various antibiotics. Therefore, antibiotic therapy is ineffective enough to treat...

Background and Aim: Methicillin-resistant Staphylococcus aureus (MRSA) has been known as a highly pathogenic bacteria in animals and humans, which is still becoming a global health issue. The prevalence of MRSA infection continues to increase worldwide and has become a global concern as a dangerous zoonotic disease. The World Health Organization es...

Background and Aim Staphylococcal enterotoxin B (SEB) is the most common serotype involved in food poisoning. The aim of this study was to develop immunoassay detection methods using a recombinant enterotoxin B antigen protein to produce recombinant polyclonal antibodies in vivo. Materials and Methods Staphylococcus aureus isolated from a food poi...

Staphylococcus aureus diketahui sering menyebabkan radang ambing pada ternak perah dan memproduksi berbagai jenis enterotoksin yang berpotensi sebagai agen penyebab keracunan makanan melalui susu maupun produk olahan susu yang dikonsumsi. Staphylococcal enterotoxin Y (SEY) asal penyakit kulit pada manusia dilaporkan tahan terhadap uji pemanasan dan...

An immune-mediated disease caused by a highly virulent Feline coronavirus (FCoV), Feline infectious peritonitis, is one of the most challenging diseases in cats due to its difficulty in either diagnosis or treatment. However, the recent investigation has offered some hope with the discovery of adenosine nucleoside analog GS-441524, which has shown...

Staphylococcus aureus is a prevalent bacterial causative agent of bovine mastitis and human food illness by secreting enterotoxins. This study aims to identify the seven classic Staphylococcus aureus enterotoxin genes (SEA to SEH) isolated from humans and animals. A total of 173 human isolates and 14 mastitis (goat and cow) isolates were amplified...

Staphylococcal enterotoxins (SE) are essential in human and animal infection and food poisoning. This study analyzed ten genes ( sek, sel, sem, sen, seo, sep, seq, ser, ses , and set ) encoding new staphylococcal enterotoxin isolated from animals and humans by multiplex polymerase chain reaction (PCR). In Yogyakarta, Indonesia, samples from human i...

Background and aim: The emergence of methicillin-resistant Staphylococcus aureus (MRSA) as a highly pathogenic strain in veterinary and human medicine is a growing global problem. This study aimed to evaluate MRSA isolates of human and animal origin against various antibiotics in Yogyakarta, Indonesia. Materials and methods: The susceptibility t...

Munculnya strain Methicillin-Resistant Staphylococcus aureus (MRSA) sebagai bakteri patogen berbahaya pada kasus medik veteriner dan manusia terus menjadi perhatian utama kesehatan masyakat. Prevalensi MRSA sebagai penyakit zoonosis berbahaya terus meningkat secara global. World Health Organization memprediksi pada tahun 2050 MRSA akan menjadi peny...

Background and Aim: Staphylococcus aureus produces various superantigen exotoxins, including staphylococcal enterotoxin B (SEB). It causes fatal anaphylactic reactions and toxic shock. This study aimed to evaluate the reaction of leukocytes and histopathological changes in the respiratory organs of Balb/c mice after intranasal infection with entero...

Background and aim: Guinea pigs (GPs) (Cavia porcellus) are not only kept as pets but also widely used in biological and biomedical research. At present, GPs are also used as a species for animal-assisted therapy (AAT). Consequently, assessing their health status is vital to determining their quality of life, usability for research, and prevention...

Sahabat Ternak is one of the Etawah crossbreed (PE) goat farm groups in Sleman. This farm group focuses on goat milk production and processed goods. Problems that commonly arise in dairy goat farms are the cases of subclinical mastitis, which are quite high. This disease may cause a decrease in milk production and quality. The mastitis subclinical...

Background and Aim: Gouty arthritis is a metabolic disorder involving monosodium urate (MSU) crystal deposition as a key initiator of acute inflammation. Dysregulation of the nucleotide-binding oligomerization domain, leucine-rich repeat, and pyrin domain-containing protein 3 (NLRP3) inflammasome is associated with the pathogenesis of gout through...

Background: Chlamydophila felis, formerly known as Chlamydia psittaci var. felis, is frequently associated with ocular, respiratory, and occasionally reproduction tract infections. Even though the infection is sometimes asymptomatic, it potentially results in a latent immunosuppressive infection. Objective: This study aimed to identify occurrenc...

Background Feline infectious peritonitis (FIP) is a fatal immune-mediated disease in cat, caused by mutated feline coronavirus (FCoV). Due to its difficulties in diagnosis, FIP is sometimes underdiagnosed. Therefore, several laboratory procedures were performed to gain high index suspicion of FIP. However, through several laboratory findings, not o...

Staphylococcus aureus (S. aureus) causing mastitis needs to be identified as the basis of treatment. This study aimed to characterize S. aureus isolated from Peranakan Ettawa (PE) goat’s milk in Pekanbaru. A total of 50 milk samples were collected from the Farm in Pekanbaru. Based on the cultural and biochemical properties and the amplification of...

p class="abstrak1">Virgin coconut oil (VCO) contains bioactive that induce immunity against infectious diseases. This study aim to determine the immunomodulatory effects of VCO based on the activity of superoxide dismutase (SOD), lymphocyte proliferation, and histopathological examination in liver and kidney of rats infected with Staphylococcus aur...

The study described the development of a haptoglobin-based diagnostic tool for mastitis in Ettawa crossbreed goats. Fifty eight milk samples were collected from a flock of goats in Yogyakarta, Central Java, Indonesia. All samples were tested for mastitis using the California Mastitis Test (CMT), Somatic Cell Count (SCC), and Polymerase Chain Reacti...

Staphylococcus aureus is recognized worldwide as a major pathogen causing subclinical intramammary infections in dairy cows and food poisoning due to its ability to produce enterotoxin. The study aimed to identify enterotoxins of S. aureus and clustering the enterotoxins based on assessory gene regulator (agr). Virulence of S. aureus to the host wa...

Genus Staphylococcus merupakan salah satu patogen bakteri penyebab mastitis yang menyebabkan kerugian ekonomi pada kambing Peranakan Etawa. Diantara Staphylococcus sp. yang dapat tumbuh dengan baik dalam susu segar, diketahui Staphylococcus aureus dapat membahayakan kesehatan manusia yang mengkonsumsi (food borne disease) karena kemampuannya dalam...

While investigating the virulence trait of Staphylococcus aureus adhering to skin of atopic dermatitis (AD) patients, we identified a novel ORF with structural similarity to superantigen from genome sequence data of an isolate from AD skin. Concurrently, the same ORF was identified in a bovine isolate of S. aureus and designated as SE l Y (H. K. On...

Antibiotics have components to inhibit infections against Staphylococcus aureus, but they depend on judicious use to minimize the incidence of resistance forms. Strategies to improve the current situation include research in finding a new antimicrobial from virgin coconut oil (VCO). The saturated fatty acid, lauric acid (LA) (C12) contain in VCO wa...

10 Bacterial infection can trigger cellular stress programs, such as production of superoxide 11 dismutase (SOD) which occurs when phagocytic activity by host cell. Virgin coconut oil 12 (VCO) has immunomodulator agents to support the homeostasis of body againts oxidative 13 stress. Twelve female Wistar as model animals to probe the effect of immun...

Electron microscopy (EM) has long been used in the discovery and description of biological specimens, including utilization to identify microorganisms/agents of infectious diseases. In this study, we performed a cost-effective and straight-forward techniques without using conventional methods for imaging of biological specimens in clinico-laborator...

Staphylococcus aureus is one of the major causative agents of mastitis in animals and a variety of human diseases such as septicemia, endocarditis, arthritis dan osteomyelitis. Infection of Methicillin-resistant S. aureus (MRSA) has been widely reported and these strains are usually resistant to multiple antibiotics. The purpose of this study was t...

Staphylococcus aureus merupakan salah satu bakteri penyebab utama mastitis. Protein-A berperan penting dalam adesi dan kolonisasi bakteri pada sel inang. Penelitian ini bertujuan untuk mengetahui hubungan aktivitas fagositosis S. aureus berdasarkan keberadaan protein-A pada media serum soft agar. Sebanyak 19 isolat S. aureus susu sapi perah asal Ja...

Dental caries is an infectious disease resulting email and dentin demineralization. Dental caries have relation with consumption of cariogenic food. But, dental caries also can be initiation with bacteria from Streptococcus class like Streptococcus mutans. Beluntas leaves (Pluchea indica L.) are a tropical herbal plant and this plant very easy to f...

Background and Objective: Streptococcus suis (S. suis) is one of the infectious agents that can cause a variety of symptoms of the disease in pigs. The Papua people live closely with pigs and the pigs raise extensively around their Honai (traditional home). The pigs are part of their way of life and part of their family. Pigs also play an important...

Neutrophil gelatinase-associated lipocalin (NGAL) is a biomarker that can potentially be used for the detection of acute renal failure earlier and more accurately than current standard examinations, such as serum levels of creatinine and urea which are less sensitive and less specific. The objective of this research was to assess the ability of NGA...

Cases of mastitis in cows at Baturraden are leading to signifi cant and ongoing problems due to reduced production and lower milk quality. This study was designed to identify which of selected virulence determinant genes of S. aureus are involved in the Baturraden infection, and to determine the clonal relationship among these isolates. Seventeen i...

Honey is natural food that common used directly by the society. Forest honey produced by wild bees (Apis dorsata), while the other type of honey produced by Apis mellifera. Beekeeping A. mellifera with monoflora or multiflora nectar is generally carried out by various countries, including Indonesia. Scientifically, honey containing bioactive compou...

Penelitian menggunakan kultur sel (in-vitro) merupakan salah satu penelitian bidang biomedis yang membutuhkan media penyimpanan dengan sifat menyerupai cairan fisiologis tubuh. Penelitian ini bertujuan menguji kemampuan air kelapa sebagai media penyimpanan untuk penelitian kultur sel. Preparasi air kelapa sebagai media kultur sel dilakukan di dalam...

Antimicrobial resistance patterns and gene encoding for methicillin/oxacillin resistance (mecA) were determined in 73 Staphylococcus aureus. The isolates of S aureus originated from bovine (39 isolates) from Peranakan Etawa (PE) or crossbred Etawa goats (24 isolates) and from patients of Sarjito hospital (10 isolates) in Indonesia. The identificati...

Village pig husbandry is an important part of livestock production in Papua Province, Eastern Indonesia. However, high level of disease and mortality constrains production. The aim of this study was to investigate the prevalence of the selected pathogens in village pigs in the Jayawijaya Region of Papua Province, Indonesia. Two studies were conduct...

This study aimed to identify staphylococcal mastitis milk in Peranakan Etawa (PE) or Etawa crossbred goats in Indonesia. Total of 93 milk samples used in this study, there were 49 (52.7%) subclinical and 3 (3.2%) clinical mastitis samples. From 52 subclinical and clinical mastitis samples could be isolated 20 (38.5%) staphylococci. Phenotypic ident...

An infectious diseases outbreak affecting pigs and monkeys in Bali in 1994, with case fatality rate (CFR) 75%. The disease widespread rapidly to the other district in Indonesia and developed serious problems. The causative agent involving the disease was Streptococcus equi subsp. zooepidemicus, a β-hemoIytic type of the streptococcus. The disease w...

Streptococcus suis is a bacterial pathogen causing disease of pigs that characterized by meningitis,bronchopneumonia, arthritis, pericarditis, polyserositis and septicaemia. S. suis especially serotype 2 caninfect human (zoonotic) with a special symptom of meningitis. The aim of this research was to detect S.suis infection based on muramidase relea...

Staphylococcus aureus is a major pathogen causing clinical and subclinical mastitis in dairy milk cows. The mastitis has immense economical impacts, where it reduces of the quantity and quality of milk production. The aims of the research were to analyse haemaglutinin and gene encoding fibronectin binding proteins. Nineteen Staphylococcus aureus is...

Streptococcus suis is well known as the causative agent of disease in various animal including pigs and human. The streptococcal cultures isolated from pigs and human have been characterized and identified biochemically and serologically as Streptococcus suis. The Cultures could be serotyped with 28 different monospecific antisera. Tne result of ti...

Staphylococcus aureus (S. aureus) is a major cause various infections in humans and animals throughout the world. The increasing incidence of S. aureus infection, particularly methicillin-resistant Staphylococcus aureus (MRSA) is complicated by the antibiotic-resistance phenomenon. Thus, it requires new strategies to prevent wider scale of its inci...

Jembrana disease virus (JDV) is a viral pathogen that causes Jembrana disease in Bali cattle (Bos javanicus) with high mortality rate. An easy and rapid diagnostic method is essential for further control this disease. We used a reverse transcription loop-mediated isothermal amplification (RT-LAMP) combined with lateral flow dipstick (LFD), based on...

Staphylococcus aureus (S. aureus) is a major cause various infections in humans and animals throughout the world. The increasing incidence of S. aureus infection, particularly methicillin-resistant Staphylococcus aureus (MRSA) is complicated by the antibiotic-resistance phenomenon. Thus, it requires new strategies to prevent wider scale of its inci...

The objective of the current survey was to provide an update on pig farming practices in the Jayawijaya region, Papua Province, Indonesia. A structured semi-close-ended questionnaire was used to interview 367 farmers across the Jayawijaya region. Results showed that farms, on average, comprised of 8.8 pigs (CI 8.5-9.1). The average litter size was...

Penelitian ini bertujuan menentukan hubungan clonal methicillin resistance Staphylococcus aureus (MRSA) antara isolat sapi dan manusia. Staphylococcus aureus yang digunakan dalam penelitian ini berasal dari infeksi kulit manusia di Yogyakarta (10 isolat) dan dari susu sapi perah (11 isolat) yang berasal dari Yogyakarta, Solo, dan Boyolali. Identifi...

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The research aimed at determining the blood profile of local rabbits infected with different dose of Eimeria magna oocysts. This research used 45 male rabbits with the age of 4 month old, range from 1.5 to 1.8 kg, clinically healthy and free from coccidiosis. The rabbits were randomly divided into 3 groups, group I as control (K-0) was given 1.0 ml...

The present study determined the genetic relationships between 41 Staphyloccocus (S.) aureus isolates from bovines, humans, and food using a single enzyme amplified fragment length polymorphism (AFLP) technique. We evaluated the prevalence of staphylococcal enterotoxin (SE) genes and other virulence gene determinants by PCR. The identification of S...

The research aimed at determining the blood profile of local rabbits infected with different dose of Eimeria magna oocysts. This research used 45 male rabbits with the age of 4 month old, range from 1.5 to 1.8 kg, clinically healthy and free from coccidiosis. The rabbits were randomly divided into 3 groups, group I as control (K-0) was given 1.0 ml...

Penelitian ini bertujuan untuk mengetahui respon usus kelinci yang diinfeksi Eimeria magna denganmengamati frekuensi dan amplitudo kontraksi peristalsis, dan jumlah neuron mienterik nitrergik usushalus. Hewan percobaan yang digunakan adalah 60 ekor kelinci jantan lokal sehat umur 5 bulan dengankisaran berat badan 1,5-1,8 kg, dan bebas koksidiosis....

Encapsulation is thought to be a critical virulence factor in streptococci of serological group B. In the present study two encapsulated low-density variants could be separated from their unencapsulated original strains by Percoll gradient centrifugation. The original strains had been isolated from human endocarditis and bovine mastitis. Type antig...

A marine bacterium associated with soft coral Sinularia polydactyla collected from Bandengan water, Jepara, North Java Sea, Indonesia, was successfully screened for antibacterial activity against pathogenic bacterium Streptococcus equi subsp. zooepidemicus K6.72 isolated from infected monkey of the island of Bali and identified based on morphologic...

The present study was designed to investigate the clonal relationship of methicillin-resistant Staphylococcus aureus (MRSA) strains isolated from different hospitals in Bulgaria. The isolates were analyzed by pulsed-field gel electrophoresis (PFGE) after Smal digestion and by PCR amplification of the genes encoding coagulase (coa), the gene segment...

In the present study 41 mucoid growing Streptococcus equi subsp. zooepidemicus strains (37 strains isolated from healthy two from diseased pigs, two strains isolated from healthy monkeys) appeared to be phenotypically and genotypically identical to mucoid growing S. equi subsp. zooepidemicus strains isolated from a previously described outbreak amo...

In the present study, 35 Staphylococcal strain isolated from milk samples of 16 cows from eight farms of three different geographic locations in Central Java, Indonesia, and from milk samples of 19 cows from 19 farms of different geographic locations in Hesse, Germany, were compared pheno- and genotypically. On the basis of cultural and biochemical...

In the present study, we report data on virulence determinants of Staphylococcus aureus from stable nasal carriers, emphasizing on the genes encoding fibronectin (fnbA, fnbB) and collagen (cna) adhesive molecules. Of the 44 S. aureus isolates included, 32 isolates (16 pairs) were cultured from the anterior nares of 16 healthy carriers, eight isolat...

The objective of this project was to produce shiitake-containing yoghurts as nutraceutical food. Preliminary analysis was conducted to measure nutrient contents of shiitake followed by evaluation of shiitake’s addition on the growth of yoghurt bacteria and probiotics. Yoghurt fermentation was conducted at 42°C until pH reached 4.5. Culture starter...

Immunoglobulin G (IgG) binding proteins on the surface of Streptococcus suis could be readily detected by direct cultivation of the bacteria on nitrocellulose membranes and subsequent treatment of the membranes with human IgG. Among the 75 S. suis isolates tested two cultures (S. suis P43, S. suis P143) caused a blue colouration of the membranes in...

Encapsulation is thought to be a critical virulence factor of Streptococcus suis. In the present study two capsular type 2 mutants of S. suis (M42 and M2) and their S. suis parent strain (89-1591) were further characterized. All three cultures reacted with group D specific antiserum whereas parent strain 89-1591 and mutant M42 but not mutant M2 rea...

This study was designed to comparatively investigate 28 S. suis cultures isolated from various pathological processes of pigs. All cultures could be identified biochemically and most of the cultures could be serogrouped with specific antiserum against Lancefield's serogroup D. Serotyping of the S. suis isolates mainly revealed capsular types 2, 1,...

Encapsulation is thought to be a critical virulence factor in streptococci of serological group B. In the present study two encapsulated low-density variants could be separated from their unencapsulated original strains by Percoll gradient centrifugation. The original strains had been isolated from human endocarditis and bovine mastitis. Type antig...

Thirteen isolates of Streptococcus suis from cats and dogs were characterised. Formamide extracts of 10 of the 13 cultures reacted with group D specific antiserum, and serotyping of the cultures with specific antisera against capsular types 1 to 28 revealed the serotypes 9, 20, 1/2, 22, 26 and 4; the remaining cultures were untypable. In addition o...

A marine bacterium associated with a soft coral Sin ularia polydactyla collected from Bandengan water, Jepara, North Java Sea, Indonesia, was successfully screened for antibacterial activity against pathogenic bacterium Streptococcus equi subsp. zooe pidemicus K6.72 isolated from infected monkey of the island of Bali and identified based on morphol...

Staphylococcus aureus is the causative agent of clinical or subclinical mastitis in dairy cows. Staphylococcus aureus cause also pathogenic diseases in human. Several hospitals reported the increase of methicillin resistant S. aureus (MRSA) frequency, because this srtain was resistant to several antibiotics. The aim of research was to characterize...