How we measure 'reads'
A 'read' is counted each time someone views a publication summary (such as the title, abstract, and list of authors), clicks on a figure, or views or downloads the full-text. Learn more
I would like to generate a very simple ImageJ macro, which first measures the area in a user-selected region of interest (ROI) and then the fluorescence signal intensity within the same ROI that is adjusted by manual thresholding.
Attached is a PDF, which shows the steps in the macro, ROIs are indicated with yellow selection.
As the ROI selection and the thresholding needs to be done manually in every image by the user, I would need this macro to ask the user after an image is opened:
“Select your region of interest” (=> I guess this could even be skipped by starting the macro after the manual selection of the ROI.)
And then again the macro should stop to have the user set the threshold.
Any suggestions how can I incorporate these into such a macro?
Thanks very much for any suggestions,
I would like to check in vivo insulin action, by assessing the IRS-1 and phospho-IRS-1, together with Akt and phospho Akt in adipose tissue after insulin administration. Any suggestions on protocols, and antibodies to purchase are welcome!
We would like to design FRET type of probes to detect the presence of alternatively spliced isoforms of certain genes. My question is regarding the optimal probe design. How long the probe should be? Would you use pure DNA probes or would you incorporate LNAs, and if yes which part of the probe (5', middle or 3')? Does it make sense to use a probe couple like FAM - Black Hole Quencher (BHQ) 1 and TAMRA - BHQ 2 for multiplexing?
I have cDNA from isolated murine macrophages (I have 4 samples from different mice) and I wanted to check the gene expression of 5 different genes. I obtained Ct values for these genes, however I am not sure how I should express these data since I am not comparing my data to any other condition (like control versus treated etc.). I thought of showing deltaCt values (difference between my target gene and my house keeping gene). Any ideas/ suggestions?