Simon Hennig

Simon Hennig
Hannover Medical School | MHH · Institute for Biophysical Chemistry

Dr.

About

25
Publications
2,540
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366
Citations
Introduction
Simon Hennig currently works at the Institute for Biophysical Chemistry, Hannover Medical School. Simon does research in Optics, Experimental Physics and Biophysics.

Publications

Publications (25)
Article
Full-text available
Super-resolution fluorescence imaging provides critically improved information about the composition, organization and dynamics of subcellular structures. Quantum-dot-triexciton imaging (QDTI) has been introduced as an easy-to-use sub-diffraction imaging method that achieves an almost 2-fold improvement in resolution when used with conventional con...
Preprint
Full-text available
Super-resolution fluorescence imaging provides critically improved information about the composition, organization and dynamics of sub-cellular structures. Quantum-Dot-Triexciton Imaging (QDTI) has been introduced as an easy-to-use sub-diffraction imaging method that achieves an almost 2-fold improvement in resolution when used with conventional co...
Article
Full-text available
Injection techniques to deliver macromolecules to cells such as microinjection have been around for decades with applications ranging from probing whole organisms to the injection of fluorescent molecules into single cells. A similar technique that has raised recent interest is nanoinjection. The pipettes used here are much smaller and allow for th...
Presentation
Full-text available
Single cell manipulation is of high interest in the biosciences, either by generating external stimuli, by injection of fluorescent probes or other molecules of interest or extraction of an intracel-lular sample. Single cell manipulation allows to follow the reaction of a specific cell, determine the formation of intracellular structures, visualize...
Method
Full-text available
has been using nanopi-pettes now for over a decade, representing key elements for scanning ion conductance microscopy (SICM), nanoinjection and nanodeposition. As essential parts for these applications, nanopipettes mediate an ionic current through liquid solution loaded inside the hollow nanopipettes and leading it through their tip aperture with...
Article
Full-text available
Insertion of foreign molecules such as functionalized fluorescent probes, antibodies, or plasmid DNA to living cells requires overcoming the plasma membrane barrier without harming the cell during the staining process. Many techniques such as electroporation, lipofection or microinjection have been developed to overcome the cellular plasma membrane...
Article
Full-text available
Super-resolved structured illumination microscopy (SR-SIM) is an important tool for fluorescence microscopy. SR-SIM microscopes perform multiple image acquisitions with varying illumination patterns, and reconstruct them to a super-resolved image. In its most frequent, linear implementation, SR-SIM doubles the spatial resolution. The reconstruction...
Data
Supplementary Figures 1-8, Supplementary Note 1 and Supplementary References
Presentation
Full-text available
A single nanopipette was used to deliver up to three fluorescent labels into individual living cells within a single injection step. The utilized nanoinjection system allows monitoring of the approach, selective staining of cellular compartments and also live tracking of the delivery process by wide-field fluorescence microscopy.
Article
We describe a method for the deposition of minute amounts of fluorophore-labeled oligonucleotides with high local precision in conductive and transparent solid layers of polyvinyl alcohol (PVA) doped with glycerin and cysteamine (PVA-G-C layers). Deposition of negatively charged fluorescent molecules was accomplished with a setup based on a scannin...
Article
We introduce a novel and universal method for fast optical high, as well as super-resolution imaging. Our method is based on reconstructing super-resolved images from conventional image sequences containing rapid random signal fluctuations. Such sequences could be obtained from either wide-field single-molecule blinking experiments or rapid image s...
Article
Optical microscopy modalities that achieve spatial resolution beyond the resolution limit have opened up new opportunities in the biomedical sciences to reveal the structure and kinetics of biological processes on the nanoscale. These methods are, however, mostly restricted to fluorescence as contrast mechanism, which limits their ultimate spatial...
Conference Paper
We demonstrate super-resolved structured illumination microscopy (SR-SIM) of Raman-active samples with 100 nm spatial resolution. By combining SR-SIM with coherent Raman scattering, even biological samples can be visualized with doubled spatial resolution.
Article
Labeling internal structures within living cells with standard fluorescent probes is a challenging problem. Here, we introduce a novel intracellular staining method that enables us to carefully control the labeling process and provides instant access to the inner structures of living cells. Using a hollow glass capillary with a diameter of <100 nm...
Article
We demonstrate a novel and easy-to-use method to dramatically reduce noise and background contributions in advanced fluorescence microscopy experiments. The underlying idea is that the entropy value increases for systems with a large number of accessible energy states. Intensity fluctuations originating from photophysical or photochemical effects l...
Article
We introduce semiconductor quantum dot-based fluorescence imaging with approximately 2-fold increased optical resolution in three dimensions as a method that allows both studying cellular structures and spatial organization of biomolecules in membranes and subcellular organelles. Target biomolecules are labelled with quantum dots via immunocytochem...
Article
We describe a simple method that improves optical resolution in fluorescence microscopy approximately 1.7-fold in all three dimensions and can be implemented on any basic confocal scanning microscope. This approach is based on three-photon absorption of commercially available quantum dots generating a triple exciton (triexciton) and subsequent blue...

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