Silvia Hernandez-Ainsa

Silvia Hernandez-Ainsa
University of Zaragoza | UNIZAR · Institute of Nanoscience and Materials of Aragon (INMA)

PhD

About

53
Publications
4,362
Reads
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1,306
Citations
Citations since 2016
24 Research Items
989 Citations
2016201720182019202020212022050100150
2016201720182019202020212022050100150
2016201720182019202020212022050100150
2016201720182019202020212022050100150
Introduction
Silvia Hernandez-Ainsa currently works at the Instituto de Nanociencia y Materiales de Aragón (INMA), CSIC-University of Zaragoza. Silvia does research in Materials Science, Biotechnology, Nanomedicine and Supramolecular Chemistry.
Additional affiliations
February 2014 - May 2017
University of Cambridge
Position
  • Fellow
March 2011 - January 2014
University of Cambridge
Position
  • PostDoc Position
January 2007 - February 2011
University of Zaragoza
Position
  • PhD
Education
September 2001 - August 2006

Publications

Publications (53)
Article
Full-text available
The design of simple and versatile synthetic routes to accomplish triggered-release properties in carriers is of particular interest for drug delivery purposes. In this context, the programmability and adaptability of DNA nanoarchitectures in combination with liposomes have great potential to render biocompatible hybrid carriers for triggered cargo...
Article
Funding Acknowledgements Type of funding sources: Public grant(s) – National budget only. Main funding source(s): Proyecto MINECO-Ministerio de Ciencia e Innovación (Spain)- PID2019-105674RB-I00 Proyecto DGA- Diputación General de Aragón (Spain)- LMP128_21 Introduction Cardiac microRNAs (miRNAs) have been found to be dysregulated in cardiac diseas...
Article
Full-text available
Gene therapy has become a relevant tool in the biomedical field to treat or even prevent some diseases. The effective delivery of genetic material into the cell remains a crucial step to succeed in this purpose. In the search for efficient non-viral vectors, a series of amino-terminated dendronized hyperbranched polymers (DHPs) of different generat...
Preprint
Full-text available
The encapsulation of chemotherapeutics by biocompatible carrier structures holds great promise to preserve their therapeutic activity and favor their delivery to tumor sites. To enhance the bioavailability of a drug at the targeted tissue, triggered release mechanisms have received increasing research interest. Many approaches rely on exogeneous tr...
Preprint
Full-text available
The encapsulation of chemotherapeutics by biocompatible carrier structures holds great promise to preserve their therapeutic activity and favor their delivery to tumor sites. To enhance the bioavailability of a drug at the targeted tissue, triggered release mechanisms have received increasing research interest. Many approaches rely on exogeneous tr...
Article
Modelling the structure and behaviour of vesicles in cells requires liposomes with precise sizes, but producing liposomes with a narrow size distribution is challenging. An approach has now been developed to accurately size-sort liposomes in a scalable way by coating them with customized structures based on DNA nanotechnology.
Article
Full-text available
The design of efficient drug-delivery vehicles remains a big challenge in materials science. Herein, we describe a novel class of amphiphilic hybrid dendrimers that consist of a poly(amidoamine) (PAMAM) dendritic core functionalized with bisMPA dendrons bearing cholesterol and coumarin moieties. Their self-assembly behavior both in bulk and in wate...
Article
Optoacoustic tomography (OT) enables non‐invasive deep tissue imaging of optical contrast at high spatio‐temporal resolution. The applications of OT in cancer imaging often rely on the use of molecular imaging contrast agents based on near‐infrared (NIR) dyes to enhance contrast at the tumor site. While these agents afford excellent biocompatibilit...
Article
Full-text available
The use of nanocarriers has been revealed as a valid strategy to facilitate drug bioavailability, and this allows for expanding the drug libraries for the treatment of certain diseases such as viral diseases. In the case of Hepatitis C, the compounds iopanoic acid and 3,3′,5-triiodothyroacetic acid (or tiratricol) were identified in a primary scree...
Article
Full-text available
For more than one hundred years, several treatments against malaria have been proposed but they have systematically failed, mainly due to the occurrence of drug resistance in part resulting from...
Preprint
Optoacoustic tomography (OT) enables non-invasive deep tissue imaging of optical contrast at high spatio-temporal resolution. The applications of OT in cancer imaging often rely on the use of molecular imaging contrast agents based on near infrared dyes to enhance contrast at the tumor site. While these agents afford excellent biocompatibility and...
Article
Full-text available
The self-assembly of the protein clathrin on biological membranes facilitates essential processes of endocytosis and has provided a source of inspiration for materials design by the highly ordered structural appearance. By mimicking the architecture of the protein building blocks and clathrin self-assemblies to coat liposomes with biomaterials, adv...
Article
Full-text available
Two novel series of ionic liquid crystal polymers that display proton conductive properties are presented here. These materials are based on linear (l-PEI) or branched (b-PEI) poly(ethyleneimine) functionalized with unsymmetrical oxadiazole carboxylic acids derived from 1,3,4-oxadiazole (1,3,4-OXAm) or 1,2,4-oxadiazole (1,2,4-OXAm). The subscript “...
Article
Full-text available
We report an active DNA construct capable of probing pH through a photoacoustic (PA) ratiometric analysis approach. Our nanoprobe enables different PA readout in tissue mimicking phantoms in the range between pH 6.8 to 7.8 at physiologically relevant sodium concentrations. Thus, it represents a promising platform to probe pH values relevant to the...
Article
Metal-organic anion channels based on Zn10L15 pentagonal prisms have been prepared by subcomponent self-assembly. The insertion of these prisms into lipid membranes was investigated by ion-current and fluorescence measurements. The channels were found to mediate the transport of Cl– anions through planar lipid bilayers and into vesicles. Toluenesul...
Article
Molecular rulers that rely on the Förster resonance energy transfer (FRET) mechanism are widely used to investigate dynamic molecular processes that occur on the nanometer scale. However, the capabilities of these fluorescence molecular rulers are fundamentally limited to shallow imaging depths by light scattering in biological samples. Photoacoust...
Article
Metal-organic ion channels based on Zn10L15 pentagonal prisms have been prepared by subcomponent self-assembly. The insertion of these prisms into lipid membranes was investigated by ion-current and fluorescence measurements. The channels were found to mediate the transport of Cl- anions through planar lipid bilayers and into vesicles. Toluenesulfo...
Technical Report
Full-text available
Molecular rulers that rely on the Förster resonance energy transfer (FRET) mechanism are widely used to investigate dynamic molecular processes that occur on the nanometer scale. However, the capabilities of these fluorescence molecular rulers are fundamentally limited to shallow imaging depths by light scattering in biological samples. Photoacoust...
Article
Full-text available
Quantifying drug permeability across lipid membranes is crucial for drug development. In addition, reduced membrane permeability is a leading cause of antibiotic resistance in bacteria, and hence there is a need for new technologies that can quantify antibiotic transport across biological membranes. We recently developed an optofluidic assay that d...
Article
We present a novel approach to reversibly control the assembly of liposomes through an anchored multistimuli responsive DNA oligonucleotide decorated with an azobenzene moiety (AZO-ON1). We show that liposomes assembly can be simultaneously controlled by three external stimuli: light, Mg(2+), and temperature. (i) Light alters the interaction of AZO...
Article
In this paper, we present the preparation and properties of some ionic PAMAM derivatives, which combine hydrophilic and hydrophobic carboxylic acid chains as counter-ions for all protonable inner and outer amino groups. The amphiphilic nature of the final ionic codendrimers and, hence, their self-assembling features can be modulated by using differ...
Article
Self-assembled DNA nanostructures have been used to create man-made transmembrane channels in lipid bilayers. Here, we present a DNA-tile structure with a nominal sub-nanometer channel and cholesterol-tags for membrane anchoring. With an outer diameter of 5 nm and a molecular weight of 45 kDa, the dimensions of our synthetic nanostructure are compa...
Article
The DNA origami technique can enable functionalization of inorganic structures for single-molecule electric current recordings. Experiments have shown that several layers of DNA molecules, a DNA origami plate, placed on top of a solid-state nanopore is permeable to ions. Here, we report a comprehensive characterization of the ionic conductivity of...
Article
DNA nanotechnology has enabled the construction of DNA origami nanopores; synthetic nanopores that present improved capabilities for the area of single molecule detection. Their extraordinary versatility makes them a new and powerful tool in nanobiotechnology for a wide range of important applications beyond molecular sensing. In this review, we br...
Article
Full-text available
Indole has diverse signalling roles, including modulation of biofilm formation, virulence and stress responses. Changes are induced by indole concentrations of 0.5-1.0 mM, similar to those found in the supernatant of Escherichia coli stationary phase culture. Here we describe an alternative mode of indole signalling that promotes the survival of E....
Article
We show DNA origami nanopores that respond to high voltages by a change in conformation on glass nanocapillaries. Our DNA origami nanopores are voltage sensitive as two distinct states are found as a function of the applied voltage. We suggest that the origin of these states is a mechanical distortion of the DNA origami. A simple model predicts the...
Article
Full-text available
The self-assembly behaviour both in the bulk and water of a series of amphiphilic dendrimers constituted by second generation PAMAM ionically functionalized with different amounts of myristic acid is shown here. The number of acids in the dendrimer determines the liquid-crystal properties and the structural parameters of their supramolecular organi...
Article
" ... DNA origami nanopores represent an emerging and powerful tool for biomedical research. It is our task now to exploit the properties that DNA nanotechnology and the flexibility of DNA chemistry offer for the design of novel DNA origami nanopores with adaptable characteristics."
Article
We combine DNA origami structures with glass nanocapillaries to reversibly form hybrid DNA origami nanopores. Trapping of the DNA origami onto the nanocapillary is proven by imaging fluorescently labeled DNA origami structures and simultaneous ionic current measurements of the trapping events. We then show two applications highlighting the versatil...
Article
Nanopores can be used to detect and analyze single molecules in solution. We have used glass nanopores made by laser-assisted capillary-pulling, as a high-throughput and low cost method, to detect a range of label-free proteins: lysozyme, avidin, IgG, β-lactoglobulin, ovalbumin, bovine serum albumin (BSA), and β-galactosidase in solution. Furthermo...
Article
Full-text available
We report a method for simultaneous ionic current measurements of single molecules across up to 16 solid state nanopore channels. Each device, costing less than $20, contains 16 glass nanopores made by laser assisted capillary pulling. We demonstrate simultaneous multichannel detection of double stranded DNA and trapping of DNA origami nanostructur...
Article
Indole is a biological molecule produced by more than 85 bacterial species. It acts as an inter-cellular signal, influencing multiple aspects of bacterial physiology and has proved to be an important factor in the transition to stationary phase. It also promotes resistance to a range of drugs and toxins and is involved in preventing plasmid instabi...
Article
Understanding membrane transport is of central importance in biology. In many cases transport across membranes is regulated by nanometre scale openings often named nanopores. For a deeper understanding and thus better control of membrane transport our ability to engineer nanopores is crucial. DNA origami offers a novel method for designing biomimet...
Article
Full-text available
We demonstrate simultaneous measurements of DNA translocation into glass nanopores using ionic current detection and fluorescent imaging. We verify the correspondence between the passage of a single DNA molecule through the nanopore and the accompanying characteristic ionic current blockage. By tracking the motion of individual DNA molecules in the...
Article
Full-text available
We report a simple and efficient way to accomplish the chemical modification of glass nanopores by means of lipid self-assembly. Lipid coating improves the success rate of these glass nanopores as biosensors to detect λ-DNA.
Conference Paper
We present here recent data showing simultaneous imaging and ionic current detection of DNA fluorescently labeled with SYTOX Orange. Using a fast Electron Multiplying CCD (EMCCD) camera, we have verified the link between ionic current translocation events and the movement of the DNA into the nanocapillary. Further work will focus on elucidating the...
Article
The synthesis and characterization of ionic liquid crystalline dendritic polymers based on the ammonium salts of commercially available poly(propylene imine) (PPI), poly(amidoamine) (PAMAM), poly(ethyleneimine) (PEI), and the fully methylated PEI (PEIMe) with 5-(4-cyanobiphenoxy)pentanoic acid (CBPA) are reported. The occurrence of proton transfer...
Article
Indole is a very important biological molecule being produced by more than 85 Gram positive and Gram negative bacterial species, including Escherichia coli. Among its several physiological functions indole is shown to stop bacterial cell division when secreted in large quantities.Here we study the effect of indole on the electrical properties of th...
Article
Two novel series of dendrimers constituted by the ionic grafting of poly(propyleneimine) PPI-(NH2)x (x = 4, 8, 16, 32, 64) and poly(amidoamine) PAMAM-(NH2)x (x = 64) with carboxylic acids bearing an oxadiazole ring have been synthesized, and their liquid crystalline properties have been investigated. Series I is generated by the ionic attachment be...
Article
Full-text available
A series of ionic amphiphilic dendrimers constituted by the grafting of poly(amidoamine) (PAMAM) of different generations (G = 0–4) with linear carboxylic acids bearing hydrophobic chains has been prepared. Their self-assembly tendency both in bulk and in water has been investigated. Almost all of the compounds present liquid crystalline behaviour...
Article
A series of codendrimers has been prepared by the grafting of poly(propylene imine) PPI-(NH2)16 (G=3) with two different carboxylic acids: the aromatic-based 5-[4′-(4-cyanophenylazo)phenyloxy]pentanoic acid (AZO) and the aliphatic 2,2-bis(undecanoyloxymethylene)propionic acid (BUPA) in different proportions. Their thermal properties, liquid crystal...
Article
A series of codendrimers constituted by the first five generations of poly(propylene imine) (PPI) ionically substituted with 2H,2H,3H,3H-perfluoroundecanoic acid (af) and undecanoic acid (ac) in a fixed ratio (1:3) (af:ac) have been synthesized and their supramolecular organization has been investigated both in the liquid crystalline state and on a...
Article
Two novel series of codendrimers constituted by the grafting of poly(propylene imine) PPI-(NH(2))(16) (G = 3) with aromatic acids substituted with fluorinated and perhydrogenated chains in different proportions have been synthesized and their liquid crystalline properties have been investigated. Series I is generated by the ionic attachment of 4-de...
Article
(Figure Presented) A molecular tug of war between non- and semifluorinated alkyl chains (see picture) occurs when they are forced together by ionic attachment to a poly(propylene imine) dendrimer. Unexpected mesomorphic properties appear as a consequence of the competition of both substituents to control the supramolecular organization of the molec...
Article
A study was conducted to investigate ionic photoresponsive AZO-codendrimer with room temperature mesomorphism and high photoinduced optical anisotropy. The codendrimer was synthesized by sonication of a solution in THF of the third generation PPI commercially available dendrimer, AZO, and BUPA in the appropriate stoichiometry. Carboxylate formation...

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Project (1)
Archived project
We are developing DNA-origami Nanostructures as novel contrast agents for Cancer Biomaging