
Silvia Greses HuertaUniversity of Valencia | UV
Silvia Greses Huerta
PhD
About
35
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Introduction
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November 2018 - present
Publications
Publications (35)
Anaerobic fermentation (AF) processes are sensitive to temperature fluctuations, which can influence the microbial activity and overall metabolic performances. Anaerobic reactors can face unforeseen temperature control failures, leading to instabilities in the process. The present study investigated the effect of two short-term temperature perturba...
Purpose
Microalgae are considered a promising source of bioenergy and high value-added products that could help face the rising demand for energy and raw materials. However, microalgae systems entail consumption of materials and energy, with potential environmental, economic, and social costs. While environmental and economic impacts have been anal...
This research assessed the effect of decoupling hydraulic retention time (HRT) and solid retention time (SRT) on the production of volatile fatty acids (VFAs) via anaerobic fermentation of beet molasses. The performance of a continuous stirred tank reactor (CSTR, STR = HTR = 30 days) and two anaerobic sequencing batch reactors (AnSBR) with decouple...
The carboxylate platform is a sustainable and cost-effective way to valorize wastes into biochemicals that replace those of fossil origin. Short-chain fatty acids (SCFAs) are intermediates generated during anaerobic fermentation (AF) and are considered high-value-added biochemicals among carboxylates. This investigation aimed to produce SCFAs throu...
Human urine has a high chemical oxygen demand (COD) content which makes anaerobic treatments potentially appropriate for the management of yellow waters, allowing for energy recovery. However, its high N content makes this treatment challenging. The present work studied the viability of performing an anaerobic digestion process for COD valorization...
Global reliance on fossil oil should shift to cleaner alternatives to get a decarbonized society. One option to achieve this ambitious goal is the use of biochemicals produced from lignocellulosic biomass (LCB). The inherent low biodegradability of LCB and the inhibitory compounds that might be released during pretreatment are two main challenges f...
Carboxylic acids have become interesting platform molecules in the last years due to their versatility to act as carbon sources for different microorganisms or as precursors for the chemical industry. Among carboxylic acids, short-chain fatty acids (SCFAs) such as acetic, propionic, butyric, valeric, and caproic acids can be biotechnologically prod...
Added-value chemicals production via food waste (FWs) valorization using open-mixed cultures is an emerging approach to replace petrochemical-based compounds. Nevertheless, the effects of operational parameters on the product spectrum remain uncertain given the wide number of co-occurring species and metabolisms. In this study, the identification o...
This study assessed a strategy for food waste (FW) valorization into multiple products by applying a cascade combination of only two anaerobic processes using open mixed cultures. FWs were firstly subjected to an anaerobic fermentation (AF) to generate an effluent rich in valuable bioproducts. The remaining solid fraction of AF effluent was further...
This investigation evaluated the effect of process pH in the metabolite spectrum generated during the anaerobic fermentation (AF) of three carbohydrate-rich wastes (residual cucumber, tomato, and lettuce). For such a purpose, two groups of AF reactors were operated in parallel. One group was operated under inherent conditions of pH upon degradation...
Dry anaerobic digestion (D-AD) generates nitrogen-rich effluents that are normally neglected in the circular bioeconomy. The high turbidity and ammonium content hamper nitrogen recovery from these effluents via biological processes, such as microalgae culture. The goal of this study was to demonstrate microalgae growth viability in high-strength D-...
Carboxylic acids, traditionally produced from fossil fuels, might be generated from renewable biomass resources via anaerobic fermentation. Considering that the microbial activity of this bioprocess is ruled by the imposed hydraulic retention time (HRT), this investigation explored the relationship between process stability and microbial community....
To properly exploit short-chain fatty acids (SCFAs) in the chemical industry, it is of foremost importance to ensure stable SCFA profile production via anaerobic fermentation (AF). The different macromolecular distribution of food wastes (FWs) used as feedstock might be crucial for process outcome. Targeting at a specific SCFAs profile and yield, t...
Unspecific microorganisms consortia are normally used in anaerobic biodegradation of solid wastes. However, these consortia can be tuned to optimally obtain determined bioproducts. In this study, high value-added products and biogas were obtained via an innovative two-stage anaerobic bioprocess from microalgae biomass. The anaerobic fermentation (A...
The organic fraction of municipal waste (OFMW), source-sorted (SS-OFMW) and non-sorted (NS-OFMW), was used as raw material for the sequential production of bioethanol and biogas. Non-isothermal and simultaneous saccharification and fermentation (NSSF) resulted in maximum ethanol concentrations of 51 g/L and 26 g/L for SS-OFMW and NS-OFMW samples, s...
Volatile fatty acids (VFAs) are platform molecules with numerous applications. They can be obtained by adjusting the operational conditions of anaerobic digestion to avoid methanogenesis while focusing on fermentative stages. There are gaps in the knowledge of how, from a life-cycle perspective, the fermentative process performs in VFAs production...
The organic fraction of municipal solid waste (OFMSW) is an appealing feedstock for bioethanol production due to its richness in cellulosic materials. After fermentation and distillation, the remaining residue constitutes a source of unconsumed carbohydrates, proteins and lipids. These macromolecules can be further used via anaerobic digestion (AD)...
Anaerobic degradation of enzymatically pretreated Chlorella vulgaris was aimed in an upflow anaerobic sludge blanket reactor (UASB) to evaluate the organic loading rate (OLR) effect on biomass valorization. Low OLRs resulted in high methane yields (171 mL CH4/g CODin) at low hydraulic retention time (HRT of 6 days). Firmicutes (35–43%), Bacteroidet...
This study assessed the feasibility of melon and watermelon residues for the co-production of short-chain fatty acids (SCFAs) and hydrogen (H2) via single-stage anaerobic fermentation. The high content of carbohydrates and readily biodegradable organic matter in the wastes, along with the acid pH (5.6–5.8) imposed in the reactors, were key factors...
Short-chain fatty acids (SCFAs) are considered building blocks for bioproducts in the so-called carboxylate platform. These compounds can be sustainably produced via anaerobic fermentation (AF) of organic substrates, such as microalgae. However, SCFAs bioconversion efficiency is hampered by the hard cell wall of some microalgae. In this study, one...
The use of anaerobic membrane bioreactor (AnMBR) technology on urban wastewater can help to alleviate droughts, by reusing the water and nutrients embedded in the effluent in agriculture (fertigation) in line with Circular Economy principles. The combination of AnMBR and fertigation reduces CO2 emissions due to the organic matter valorization and t...
Volatile fatty acids (VFAs) are regarded as building blocks with a wide range of applications, including biofuel production. The traditional anaerobic digestion used for biogas production can be alternatively employed for VFAs production. The present study aimed at maximizing VFAs productions from Chlorella vulgaris through anaerobic digestion by a...
This study evaluated the feasibility of the anaerobic digestion as a sustainable valorisation strategy for volatile fatty acids production from agroindustrial waste (cucumber, tomato and lettuce). High bioconversion efficiencies were reached by operating the reactors at 25 °C, 3 g VS·d-1·L-1 with pH adjustment. Cucumber fermentation achieved the hi...
The potential of microbial communities for efficient anaerobic conversion of raw microalgae was evaluated in this work. A long-term operated thermophilic digester was fed with three different Organic Loading Rates (OLR) (0.2, 0.3 and 0.4 g·L−1·d−1) reaching 32–41% biodegradability values. The microbial community analysis revealed a remarkable prese...
Anaerobic digestion of indigenous Scenedesmus spp. microalgae was studied in continuous lab-scale anaerobic reactors at different temperatures (35 °C and 55 °C), and sludge retention time - SRT (50 and 70 days). Mesophilic digestion was performed in a continuous stirred-tank reactor (CSTR) and in an anaerobic membrane bioreactor (AnMBR). Mesophilic...
Biogas production from lignocellulosic biomass is generally considered to be challenging due to the recalcitrant nature of this biomass. In this study, the recalcitrance of birch was reduced by applying steam-explosion (SE) pretreatment (210 °C and 10 min). Moreover, bioaugmentation with the cellulolytic bacterium Caldicellulosiruptor bescii was ap...
Microbial communities were thoroughly characterized in a mesophilic anaerobic membrane bioreactor (AnMBR) and a thermophilic continuous stirred tank reactor (CSTR), which were both treating recalcitrant microalgal biomass dominated by Scenedesmus. 16S rRNA amplicon sequencing analysis was performed when the AnMBR achieved 70% algal biodegradation a...