Silvia Albillos

Silvia Albillos
Universidad de Burgos | UBU · Department of Biotechnology and Food Science

PhD

About

43
Publications
13,452
Reads
How we measure 'reads'
A 'read' is counted each time someone views a publication summary (such as the title, abstract, and list of authors), clicks on a figure, or views or downloads the full-text. Learn more
946
Citations
Citations since 2017
8 Research Items
597 Citations
2017201820192020202120222023020406080100
2017201820192020202120222023020406080100
2017201820192020202120222023020406080100
2017201820192020202120222023020406080100
Introduction
My reserach interests are: Food allergens. Immunoassays for protein detection. Protein chemistry. ELISA of all kinds, Western blot, allergen stability studies via protein aggregation, circular dichroism, hydrophobicity, fluorescence, light scattering (DSL), differential scanning calorimetry (DSC). Reduction of allergenicity. Chemometrics. Protein stability and protein structure. Metabolomics. Enzymatic acitivy. Parkinson´s disease.
Additional affiliations
May 2013 - present
Universidad de Burgos
January 2008 - May 2013
Instituto de Biotecnología de León (INBIOTEC)
January 2005 - July 2008
Illinois Institute of Technology
Position
  • National Center for Food Safety and Technology-IIT-FDA
Education
June 1999 - November 2003
Universidad de Burgos
Field of study
  • Biochemistry and Molecular Biology
September 1997 - June 1999
Universidad de Burgos
Field of study
  • Biochemistry and Molecular Biology
September 1992 - June 1997
Universidad de Burgos
Field of study
  • Chemistry

Publications

Publications (43)
Article
Full-text available
Plant biomass is a promising substrate for biorefinery, as well as a source of bioactive compounds, platform chemicals, and precursors with multiple industrial applications. These applications depend on the hydrolysis of its recalcitrant structure. However, the effective biological degradation of plant cell walls requires several enzymatic groups a...
Article
Full-text available
In this study, the use of spray-drying technology for encapsulating Flavourzyme® (protease-peptidase complex) was evaluated to overcome the limitations (low encapsulation efficiency and no large-scale production) of other encapsulation processes. To the best of our knowledge, spray drying has not been applied previously for the immobilization of th...
Article
Full-text available
Monoacylglycerols (MAG) containing polyunsaturated fatty acids (PUFA) have been synthesized by glycerolysis of anchovy oil using Lipozyme RM-IM in a solvent-free system. The experimental conditions, reaction temperature , substrate molar ratio (glycerol/triacylglycerol, Gly/TAG) and enzyme concentration were studied. A response surface methodology...
Article
Background and purpose Given the overlapping clinical manifestations and pathology, the differentiation between Essential tremor (ET) and Parkinson´s disease (PD) is difficult. Our aims were to examine the plasma metabolomics profiling and their association with motor and non-motor symptoms (NMS) in patients with PD, and to determine differences be...
Article
Full-text available
Background: Studies have revealed controversial results regarding the diagnostic accuracy of plasma α-synuclein levels in patients with Parkinson's disease (PD). This study was aimed to analyze the diagnostic accuracy of plasma α-synuclein in PD versus healthy controls and patients with essential tremor (ET). Methods: In this cross-sectional stu...
Article
Full-text available
In this work, we identified glucose and glycerol as tacrolimus repressing carbon sources in the important species Streptomyces tsukubaensis. A genome-wide analysis of the transcriptomic response to glucose and glycerol additions was performed using microarray technology. The transcriptional time series obtained allowed us to compare the transcripto...
Article
The traceability of olive oil is an unresolved issue that remains a challenge. In this field, DNA-based techniques are very powerful tools for discrimination that are less negatively influenced by environmental conditions than other techniques. More specifically, quantitative real time PCR (qPCR) achieves a high degree of sensitivity, although the...
Article
Full-text available
Background Some types of flavonoid intermediates seemed to be restricted to plants. Naringenin is a typical plant metabolite, that has never been reported to be produced in prokariotes. Naringenin is formed by the action of a chalcone synthase using as starter 4-coumaroyl-CoA, which in dicotyledonous plants derives from phenylalanine by the action...
Article
Full-text available
Coeliac disease (CD) is an immune-mediated enteropathy resulting from exposure to gluten in genetically predisposed individuals. Gluten proteins are partially digested by human proteases generating immunogenic peptides that cause inflammation in patients carrying HLA-DQ2 and DQ8 genes. Although intestinal dysbiosis has been associated with patients...
Article
A vicilin-like globulin seed storage protein, termed convicilin, was isolated for the first time from Korean pine (Pinus koraiensis). SDS-PAGE analysis revealed that Korean pine convicilin was post-translationally processed. The N-terminal peptide sequences of its components were determined. These peptides could be mapped to a protein translated fr...
Article
Gluten, a common component in the human diet, is capable of triggering coeliac disease pathogenesis in genetically predisposed individuals. Although the function of human digestive proteases in gluten proteins is quite well known, the role of intestinal microbiota in the metabolism of proteins is frequently underestimated. The aim of this study was...
Article
In Penicillium chrysogenum the beta-lactam biosynthetic pathway is compartmentalized. This fact forces the occurrence of transport processes of penicillin-intermediate molecules across cell membranes. Many aspects around this molecular traffic remain obscure but are supposed to involve transmembrane transporter proteins. In the present work, an in-...
Article
A single gene cluster of Penicillium chrysogenum contains genes involved in the biosynthesis and secretion of the mycotoxins roquefortine C and meleagrin. Five of these genes have been silenced by RNAi. Pc21g15480 (rds) encodes a nonribosomal cyclodipeptide synthetase for the biosynthesis of both roquefortine C and meleagrin. Pc21g15430 (rpt) encod...
Article
Full-text available
The food industry is concerned about the presence of hidden allergens or undesired protein residues in final products and has to take measures to comply with current food allergen-labelling regulations. It is known that thermal processing decreases protein solubility and hinders the accuracy of their quantitation. The objective of this study was to...
Article
Full-text available
Filamentous fungi produce an impressive variety of secondary metabolites; many of them have important biological activities. The biosynthesis of these secondary metabolites is frequently induced by plant-derived external elicitors and appears to also be regulated by internal inducers, which may work in a way similar to that of bacterial autoinducer...
Article
Full-text available
Alkaline phosphatase is a ubiquitous milk enzyme that historically has been used to verify adequate pasteurization of milk for public health purposes. Current approved methods for detection of alkaline phosphatase in milk include the use of enzyme photoactivated substrates to give readings in milliunits per liter. The U.S. and European public healt...
Article
Blue cheeses are produced from cow's milk, sheep's milk, or goat's milk depending on the variety. They always have Penicillium cultures added, along with the lactic acid bacteria starter cultures (Lactococcus lactis and Leuconostoc cremoris), so that the final product is spotted or veined throughout with blue, blue-gray or blue-green mold. The afte...
Chapter
Full-text available
Blue cheeses are produced from cow's milk, sheep's milk, or goat's milk depending on the variety. They always have Penicillium cultures added, along with the lactic acid bacteria starter cultures (Lactococcus lactis and Leuconostoc cremoris), so that the final product is spotted or veined throughout with blue, blue-gray or blue-green mold. The afte...
Article
Seed storage proteins are accumulated during seed development and act as a reserve of nutrition for seed germination and young sprout growth. Plant seeds play an important role in human nutrition by providing a relatively inexpensive source of protein. However, many plant foods contain allergenic proteins, and the number of people suffering from fo...
Article
Information relating to the resistance of food allergens to thermal and/or chemical denaturation is critical if a reduction in protein allergenicity is to be achieved through food-processing means. This study examined the changes in the secondary structure of an almond allergen, amandin, and its acidic and basic polypeptides as a result of thermal...
Article
Pine nuts are economically important as a source of human food. They are also of medical importance because numerous pine nut allergy cases have been recently reported. However, little is known about the proteins in pine nuts. The purpose of this study was to purify and characterize pine nut storage proteins. Reported here is the first detailed pur...
Article
The 11S globulins from plant seeds account for a number of major food allergens. Because of the interest in the structural basis underlying the allergenicity of food allergens, we sought to crystallize the main 11S seed storage protein from almond ( Prunus dulcis). Prunin-1 (Pru1) was purified from defatted almond flour by water extraction, cryopre...
Article
Full-text available
Food allergies affect an estimated 10 to 12 million people in the United States. Some of these individuals can develop life-threatening allergic reactions when exposed to allergenic proteins. At present, the only successful method to manage food allergies is to avoid foods containing allergens. Consumers with food allergies rely on food labels to d...
Article
Capillary electrophoresis using an untreated fused-silica capillary and a low-pH buffer containing urea and hydroxypropyl methyl cellulose was used to follow the proteolytic action of neutral protease of Bacillus subtilis on isolated casein fractions and whole milk casein (CN) samples. Electrophoretic analysis showed that this protease caused inten...
Article
The effect of the ripening time on the proteolytic process in cheeses made from ewe's milk during a 139-day ripening period was monitored by the use of capillary electrophoresis of pH 4.6 insoluble fraction. Totals of 18 and 21 peaks were recognized and matched in the electropherograms obtained with a fused-silica capillary and a neutral capillary...
Article
The effect of the ripening time on the proteolytic process in cheeses manufactured from mixtures of cow's and ewe's milk during a 167-day ripening period was monitored by capillary electrophoresis of the pH 4.6-insoluble fraction. Totals of 21 and 16 peaks were recognized and matched in the electropherograms obtained with a fused-silica capillary a...
Article
Central composite design and response surface methods were applied to optimise and assess the robustness of a capillary zone electrophoresis method for the separation of bovine caseins. Buffer pH, running voltage and polymeric additive buffer concentration were the parameters selected for this study. Successful results were obtained at 18.5 kV, wit...
Article
Full-text available
La proteolisis de las caseínas es el fenómeno más importante para cuantificar el grado de maduración del queso. En esta Tesis se aplica el diseño experimental para optimizar la separación de caseínas mediante electroforesis capilar (EC), sobre muestras de leche de vaca y oveja; se realiza un seguimiento temporal de los productos liberados por hidró...

Network

Cited By

Projects

Project (1)
Project