Sigrid Zink

Protein mannosyltransferases (Pmt proteins) initiate O glycosylation of secreted proteins in fungi. We have characterizedPMT6, which encodes the second Pmt protein of the fungal pathogen Candida albicans. The residues of Pmt6p are 21 and 42% identical to those of C. albicans Pmt1p and S. cerevisiae Pmt6p, respectively. Mutants lacking one or twoPMT...

Due to the high permeability of endothelial cell layers derived from macrovascular vessels, precise determination of their barrier function towards ion movement requires refined experimental techniques. We thus cultured bovine aortic endothelial cells (BAEC) directly on thin gold-film electrodes and measured the electrochemical impedance to study t...

There is much evidence that diabetes and hyperglycaemia contribute to the impairment of endothelial function and induce severe changes in the proliferation, the adhesive and synthetic properties of endothelial cells. Induction of apoptosis could represent one mechanism to prevent the new accumulation of those vascular defects and to allow generatio...

Migration of the fungal pathogen Candida albicans across the endothelial cell layer is considered a prerequisite for the invasion of multiple organs occurring in systemic candidiasis. We developed an experimental system in which C. albicans migrates from a luminal compartment across a monolayer of bovine aortic endothelial cells on a porous filter...

Barrier function of endothelial cells (EC) was modulated using beta-adrenergic agonists, e.g., isoproterenol (ISO) and formoterol (FOR). To get a direct comparison between EC from different vascular sources, we isolated EC from aorta (BAEC) and retina (BREC) of the same calf. For permeability studies, EC were cultured on polycarbonate filters. At c...

In vascular endothelium, L-arginine (ARG) plays a crucial role as a substrate for various metabolic pathways, one of which is the synthesis of bioregulatory nitric oxide. Transport of ARG across the cell membrane determines intracellular substrate availability. Membranous transport in turn may be dependent on the extra-to-intracellular gradient in...

The barrier function of cultured, macrovascular endothelial cells derived from bovine aorta was analyzed using confluent monolayers of cells and measuring the exchange of fluorescein dextrans of different molecular masses. The effects of beta-adrenoceptor agonists with different selectivity for beta 1- and beta 2-adrenoceptors (AR) were investigate...

The barrier function of cultured, macrovascular endothelial cells derived from bovine aorta was analyzed using confluent monolayers of cells and measuring the exchange of fluorescein dextrans of different molecular masses. The effects of β-adrenoceptor agonists with different selectivity for and (AR) were investigated. Formoterol, a novel high-affi...

Artmann, GM., S. Zink, G.Schneider, P. Rösen: A new approach to investigate endothelial cell integrity. 8th Int. Congr of Biorheology, Yokohama. Biorheology, 29(1): 96, 1992

Our previous research on the pathological reaction of the vessel wall to arterial hypertension (stages of hypertension: Ischijima, 1969) and its dependence on age and vessel in SHR has included EM studies on the walls of the aorta, and the coronary, basilar, renal, femoral and testicular arteries. Differences dependent upon age and region were foun...