Sigrid März

Freelancer

Purpose: Recently, a new marker protein for microglial cells in the brain was postulated, transmembrane protein 119 (TMEM119), raising the hope for a new opportunity to reliably and unambiguously detect microglial cells in histologic sections. It was of interest whether TMEM119 also was a reliable microglial marker in the retina. Methods: Anti-T...

Actin-binding proteins are essential for linear and branched actin filament dynamics that control shape change, cell migration, and cell junction remodeling in vascular endothelium (endothelial cells [ECs]). The epithelial protein lost in neoplasm (EPLIN) is an actin-binding protein, expressed as EPLIN-α and EPLIN-β by alternative promoters; howeve...

Actin-binding proteins are essential for linear and branched actin filament dynamics that control shape change, cell migration, and cell junction remodeling in vascular endothelium (endothelial cells [ECs]). The epithelial protein lost in neoplasm (EPLIN) is an actin-binding protein, expressed as EPLIN-α and EPLIN-β by alternative promoters; howeve...

VEGFR-2/Notch signalling regulates angiogenesis in part by driving the remodelling of endothelial cell junctions and by inducing cell migration. Here, we show that VEGF-induced polarized cell elongation increases cell perimeter and decreases the relative VE-cadherin concentration at junctions, triggering polarized formation of actin-driven junction...

Characterization of endothelial cell contacts in the mouse model of laser-induced neovascularisation, expression of cytokine receptor VEGFR2 in newly developed vessels.

Key Points Only CD99 on endothelial cells, not on neutrophils, participates in neutrophil extravasation in vivo. A new function was found for CD99: support of chemokine-induced β2-integrin activation and neutrophil arrest by binding to PILR.

Question: Stability of endothelial cell contacts is crucial for maintaining the barrier function of the blood vessel wall. The balance of both maintaining cell junction integrity and partial opening of junctions in certain pathologies is a highly dynamic and regulated process. Vascular endothelial cadherin (VE-cadherin) together with catenins inter...

Molekulare Charakterisierung von endothelialen Zellkontakten in laser-induzierten Gefäßneubildungen in der Netzhaut

Stability of endothelial cell contacts is crucial for maintaining the barrier function of the blood vessel wall. Additionally, endothelial cell integrity needs to be loosened to enable leukocyte transcytosis to sites of inflammation. Therefore, the endothelium developed multiple mechanisms that control composition and regulation of endothelial junc...

CD99-like 2 (CD99L2) is a membrane protein with moderate sequence homology to CD99, which initiates cell aggregation of transfected cells and that is strongly expressed on endothelial cells, neutrophils, and lymphocytes. We showed recently that Abs against CD99L2 inhibit neutrophil, but not T lymphocyte, recruitment into inflamed tissues. In this s...

Leukocyte extravasation depends on various adhesion receptors at endothelial cell contacts. Here we have analyzed how mouse CD99 and CD99L2 cooperate with PECAM-1. We found that antibodies against mouse CD99 and PECAM-1 trap neutrophils between endothelial cells in in vitro transmigration assays. A sequential function, as has been suggested for hum...

CD99 is a long-known leukocyte antigen that does not belong to any of the known protein families. It was recently found on endothelial cells, where it mediates transendothelial migration of human monocytes and lymphocyte recruitment into inflamed skin in the mouse. Here, we show that CD99L2, a recently cloned, widely expressed antigen of unknown fu...

The bicistronic MOCS1 gene encodes two enzymatic activities that are necessary for the biosynthesis of the molybdenum cofactor (MoCo). Mutations in either of the two consecutive open reading frames are responsible for the majority of MoCo deficiency cases and result in a complementation group A phenotype. Two cDNA sequences have been described, whi...

Gephyrin was originally identified as a membrane-associated protein that is essential for the postsynaptic localization of receptors for the neurotransmitters glycine and GABAA. A sequence comparison revealed homologies between gephyrin and proteins necessary for the biosynthesis of the universal molybdenum cofactor (MoCo). Because gephyrin express...