Sidi Bencherif

Sidi Bencherif
Harvard University, Northeastern University, UTC Sorbonne University

Ph.D.

About

90
Publications
30,057
Reads
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6,797
Citations
Introduction
My research requires a cross disciplinary approach where aspects of engineering, chemistry, and biology are applied to the fields of tissue engineering, drug delivery, and regenerative medicine.
Additional affiliations
August 2009 - present
Harvard University
Position
  • Research Associate
January 2005 - July 2009
Carnegie Mellon University
Position
  • PhD Student
April 2002 - January 2005
National Institute of Standards and Technology
Position
  • Guest Researcher

Publications

Publications (90)
Article
Recent advances in materials science and engineering highlight the importance of designing sophisticated biomaterials with well-defined architectures and tunable properties for emerging biomedical applications. Click chemistry, a powerful method allowing specific and controllable bioorthogonal reactions, has revolutionized our ability to make compl...
Article
Cardiovascular diseases (CVDs) are known as the major cause of death worldwide. In spite of tremendous advancements in medical therapy, the gold standard for CVD treatment is still transplantation. Tissue engineering, on the other hand, has emerged as a pioneering field of study with promising results in tissue regeneration using cells, biological...
Article
Full-text available
Breast cancer is a major health concern worldwide and is the leading cause of cancer-related death among American women. Traditional therapies, such as surgery, chemotherapy, and radiotherapy, are usually ineffective. Furthermore, cancer recurrence following targeted therapy often results from acquired drug resistance. Therefore, more realistic tum...
Chapter
Full-text available
Immunotherapy has become a powerful clinical strategy to improve the survival benefit and quality of life for patients with advanced-stage cancer. Yet the therapeutic efficacy of cancer immunotherapies remains modest, with only a fraction of patients responding favorably to these treatments. Moreover, the systemic delivery of immunotherapeutics has...
Article
Full-text available
Synthetic cancer vaccines may boost anticancer immune responses by co-delivering tumor antigens and adjuvants to dendritic cells (DCs). The accessibility of cancer vaccines to DCs and thereby the delivery efficiency of antigenic material greatly depends on the vaccine platform that is used. Three-dimensional scaffolds have been developed to deliver...
Cover Page
Full-text available
In article number 2102234, Sidi A. Bencherif and co-workers use injectable oxygen-generating cryogels (O2-cryogels) as a strategy to boost antitumor immune responses. These noninvasive biomaterials are designed to controllably release oxygen, reverse hypoxia-driven immunosuppression, and promote the infiltration of pre-existing immune-fighting immu...
Article
Full-text available
Severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) has led to an unprecedented global health crisis, resulting in a critical need for effective vaccines that generate protective antibodies. Protein subunit vaccines represent a promising approach but often lack the immunogenicity required for strong immune stimulation. To overcome this cha...
Article
Full-text available
COVID-19 Vaccines In article number 2100316 by Sidi A. Bencherif and co-workers, it is demonstrated that advanced biomaterials can be leveraged to boost the effectiveness of SARS-CoV-2 protein subunit vaccines. Illustration depicting a subcutaneously injected oxygen-releasing cryogel-based COVID-19 vaccine boosting the immune response, leading to a...
Article
Full-text available
Solid tumors are protected from antitumor immune responses due to their hypoxic microenvironments. Weakening hypoxia‐driven immunosuppression by hyperoxic breathing of 60% oxygen has shown to be effective in unleashing antitumor immune cells against solid tumors. However, efficacy of systemic oxygenation is limited against solid tumors outside of l...
Article
Full-text available
Oxygen-Generating Cryogels In article number 2102234, Sidi A. Bencherif and co-workers use injectable oxygen-generating cryogels (O2-cryogels) as a strategy to boost antitumor immune responses. These noninvasive biomaterials are designed to controllably release oxygen, reverse hypoxia-driven immunosuppression, and promote the infiltration of pre-ex...
Article
Microneedle arrays (MNAs) have been used for decades to deliver drugs transdermally and avoid the obstacles of other delivery routes. Hydrogels are another popular method for delivering therapeutics because they provide tunable, controlled release of their encapsulated payload. However, hydrogels are not strong or stiff, and cannot be formed into c...
Article
Full-text available
With the emergence of the coronavirus disease 2019 (COVID-19), the world is experiencing a profound human health crisis. The number of infections and deaths due to the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) continues to increase every minute, pinpointing major shortcomings in our ability to prevent viral outbreaks. Although se...
Preprint
Full-text available
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has led to an unprecedented global health crisis, resulting in a critical need for effective vaccines that generate protective antibodies. Protein subunit vaccines represent a promising approach but often lack the immunogenicity required for strong immune stimulation. To overcome this cha...
Article
Full-text available
Despite the undeniable success of vaccination programs in preventing diseases, effective vaccines against several life-threatening infectious pathogens such as human immunodeficiency virus are still unavailable. Vaccines are designed to boost the body's natural ability to protect itself against foreign pathogens. To enhance vaccine-based immunother...
Article
Full-text available
Porous three-dimensional hydrogel scaffolds have an exquisite ability to promote tissue repair. However, because of their high water content and invasive nature during surgical implantation, hydrogels are at an increased risk of bacterial infection. Recently, we have developed elastic biomimetic cryogels, an advanced type of polymeric hydrogel, tha...
Article
Full-text available
Traumatic joint injuries can result in significant cartilage defects, which can greatly increase the risk of osteoarthritis development. Due to the limited self-healing capacity of avascular cartilage, tissue engineering approaches are required for filling defects and promoting cartilage regeneration. Current approaches utilize invasive surgical pr...
Article
Full-text available
The success of skin tissue engineering for deep wound healing relies predominantly on the design of innovative and effective biomaterials. This study reports the synthesis and characterization of a new type of naturally-derived and macroporous interpenetrating polymer network (IPN) for skin repair. These biomaterials consist of a biologically activ...
Preprint
Full-text available
Solid tumors are protected from antitumor immune responses due to their hypoxic microenvironments. Weakening hypoxia-driven immunosuppression by hyperoxic breathing of 60% oxygen has shown to be effective in unleashing antitumor immune cells against solid tumors. However, efficacy of systemic oxygenation is limited against solid tumors outside of l...
Article
Full-text available
Over the past decade, electroconductive hydrogels, integrating both the biomimetic attributes of hydrogels and the electrochemical properties of conductive materials, have gained significant attention. Hydrogels, three-dimensional and swollen hydrophilic polymer networks, are an important class of tissue engineering (TE) scaffolds owing to their mi...
Article
Full-text available
A number of drawbacks in skin grafting for wound healing have drawn researchers to focus on skin tissue engineering as an alternative solution. The core idea of tissue engineering is to use scaffolds, cells, and/or bioactive molecules to help the skin to properly recover from injuries. Over the past decades, the field has significantly evolved, dev...
Article
Full-text available
Lack of suitable auto/allografts has been delaying surgical interventions for the treatment of numerous disorders and has also caused a serious threat to public health. Tissue engineering could be one of the best alternatives to solve this issue. However, deficiency of oxygen supply in the wounded and implanted engineered tissues, caused by circula...
Article
Full-text available
Back Cover: In article number 1900824 by Sidi A. Bencherif and co‐workers, the effect of polymer concentration on the properties of autoclavable cryogels made from several naturally derived polymer precursors is reported for the first time. The structural and physical properties of autoclaved cryogels are thoroughly investigated. The results show t...
Article
Full-text available
Biomaterial sterilization is a prerequisite prior to patient's use, especially for scaffold implantation or injection. Various sterilization processes are mandated by the Food and Drug Administration including high‐pressure steam sterilization. Although high‐pressure steam or autoclave sterilization eliminates pathogens, it often leads to irreversi...
Chapter
Full-text available
Current healthcare practices often involve insertion of foreign devices into the body. Infections related to such devices or procedures pose a major threat to patient safety. As a result, there is an urgent need to develop technologies that better control healthcare-associated infections. In this regard, the latest innovations in hydrogel fabricati...
Conference Paper
Introduction: Hypoxia, defined as low oxygen tension, is a characteristic feature of solid tumors and a hallmark of aggressive cancers. The rapid growth of tumors often results in the development of a hypoxic microenvironment, leading to tumor cell growth and invasion, resistance to apoptosis, and multidrug resistance. Multicellular tumor spheroids...
Article
Full-text available
Cryogenic processes are increasingly being utilized to create unique polymeric materials that tackle challenges mainly in the biomedical arena, environmental science, and field of food technology[...]
Article
To prevent postoperative complications, there has been a substantial interest in designing syringe-injectable hydrogels. To date, cryogels remain the only viable option for preformed and large-scale hydrogels to be delivered through a conventional needle-syringe injection. Cryogels, a type of hydrogel with exceptional features, are fabricated at su...
Article
We demonstrate the application of xanthommatin (Xa), a biochrome present in arthropods and cephalopods, as an alternative chemical UV-filter that is cytocompatible while maintaining photostability and photoprotective properties.
Article
In article number 1900679 by Sidi A. Bencherif and co‐workers, a scanning electron micrograph is shown displaying Pseudomonas aeruginosa, rod‐shaped bacteria, nested on the polymer walls of a cryogel scaffold. The cryogel is made out of hyaluronic acid, a naturally‐occurring biopolymer. To enhance visualization, bacteria are pseudo‐colored in blue...
Article
Dopamine (DA) plays several important roles in the brain and body and has recently been used as a bioadhesive precursor for medical applications. However, DA oxidizes immediately when exposed to oxygen and rapidly polymerizes into polydopamine (PDA), leading to oxidative stress, cytotoxicity, and loss of DA functionalities. As a result, preventing...
Article
Full-text available
Recently, there have been huge advancements in the field of nanobiotechnology. One of the main drivers has been the development of novel nanomaterials. One new class of materials is graphene and its derivatives recognized for their novel properties present on the nanoscale. In particular, graphene and graphene-based nanomaterials have been shown to...
Article
Full-text available
Prior to any clinical application, terminal sterilization of biomaterials is a critical process imposed by the Food and Drug Administration. Of all the methods available for sterilization, high‐pressure steam sterilization such as autoclaving is the most widely used. While autoclave sterilization minimizes pathogen contamination, it can dramaticall...
Article
Electrospinning is a versatile technique used to create native tissue-like fibrous scaffolds. Recently, it has gained a large amount of attention for generation of bioactive dressing materials suitable for treatment of both chronic and acute wounds. In this review, we focus on the latest advances made in application of electrospun scaffolds for bio...
Article
Full-text available
Polymeric scaffolds such as hydrogels can be engineered to restore, maintain, or improve impaired tissues and organs. However, most hydrogels require surgical implantation that can cause several complications such as infection and damage to adjacent tissues. Therefore, developing minimally invasive strategies is of critical importance for these pur...
Article
Full-text available
Chitin and lignin primarily accumulate as bio-waste resulting from byproducts of crustacean crusts and plant biomass. Recently, their use has been proposed for diverse and unique bioengineering applications, amongst others. However, their weak mechanical properties need to be improved in order to facilitate their industrial utilization. In this pap...
Article
A covalently crosslinked methacrylated (MA)-alginate cryogel vaccine has been previously shown to generate a potent response against murine melanoma, but is not mechanically robust and requires a large 16G needle for delivery. Here, covalent and ionic crosslinking of cryogels are combined with the hypothesis that this will result in a tough MA-algi...
Article
Full-text available
Interpenetrating polymer networks (IPNs) have gained great attention for a number of biomedical applications due to their improved properties compared to individual components alone. In this study, we investigated the capacity of newly-developed naturally-derived IPNs as potential biomaterials for tissue engineering. These IPNs combine the biologic...
Article
Full-text available
Context The FibriDerm project aims at the development and usage of fibrin-based biomaterials, with mechanical properties adapted to new applications. Methods These materials are elaborated from interpenetrating polymer networks in which a fibrin-based gel, obtained through enzymatic hydrolysis of fibrinogen, is associated with a synthetic polymeri...
Article
Full-text available
The ultimate goal in skin tissue regeneration is to develop artificial skin replacements for the restoration of damaged or missing skins in patients as well as to enhance wound healing processes. Fibrin, a naturally-occurring biopolymer involved in wound healing has seen widespread use in tissue engineering due to its bioactivity, biocompatibility,...
Article
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Electrospun micro- and nanofibrous poly(glycerol sebacate)-poly(ε-caprolactone) (PGS-PCL) substrates have been extensively used as scaffolds for engineered tissues due to their desirable mechanical properties and their tunable degradability. In this study, we fabricated micro/nanofibrous scaffolds from a PGS-PCL composite using a standard electrosp...
Article
In this study, we investigated various highly porous extracellular matrix (ECM)-based cryogels for cartilage tissue engineering. For the fabrication of ECM-based cryogels, either methacrylated chondroitin sulfate (MeCS) or methacrylated hyaluronic acid (MeHA) were cross-linked along with poly (ethylene glycol) diacrylates (PEGDA) via free radical p...
Article
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Natural extracellular matrices (ECMs) are viscoelastic and exhibit stress relaxation. However, hydrogels used as synthetic ECMs for three-dimensional (3D) culture are typically elastic. Here, we report a materials approach to tune the rate of stress relaxation of hydrogels for 3D culture, independently of the hydrogel's initial elastic modulus, deg...
Article
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A biomaterial-based vaccination system that uses minimal extracorporeal manipulation could provide in situ enhancement of dendritic cell (DC) numbers, a physical space where DCs interface with transplanted tumour cells, and an immunogenic context. Here we encapsulate GM-CSF, serving as a DC enhancement factor, and CpG ODN, serving as a DC activatin...
Article
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10 We investigate ionic partition of negatively charged molecular probes into also negatively charged, covalently crosslinked alginate hydrogels. The aim is to delimit the domain of validity of the major nanoelectrostatic models, and in particular to assess the influence of hydrogel chain mobility on ionic partition. We find that the widely used Gi...
Article
Full-text available
Studies of cellular mechanotransduction have converged upon the idea that cells sense extracellular matrix (ECM) elasticity by gauging resistance to the traction forces they exert on the ECM. However, these studies typically utilize purely elastic materials as substrates, whereas physiological ECMs are viscoelastic, and exhibit stress relaxation, s...
Article
Three-dimensional macroporous scaffolds have extensively been studied for cell-based tissue engineering but their use is mostly limited to mechanical support for cell adhesion and growth on the surface of macropores. Here, a templated fabrication method is described to prepare cell-friendly inverse opal-like hydrogels (IOHs) allowing both cell enca...
Article
Millimeter to centimeter-sized injectable neural scaffolds based on macroporous cryogels are presented. The polymer-scaffolds are made from alginate and carboxymethyl-cellulose by a novel simple one-pot cryosynthesis. They allow surgical sterility by means of autoclaving, and present native laminin as an attachment motive for neural adhesion and ne...
Article
Electrically responsive hydrogels are created with interconnected macropores, which greatly enhance their ability to rapidly undergo volumetric collapse when subjected to moderate electric fields. When optimized, these electrogels are easily integrated into arrays capable of rapid configurational and chromatic optical modulations, and when loaded w...
Article
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We show novel millimeter-sized injectable neural scaffolds based on cryogels exhibiting interconnected macropores, for neural tissue engineering applications. The polymer-scaffolds are designed to be compressible, allow cell adherence and it injection through a syringe needle while preserving neural cells viability and neurite integrity. The system...
Article
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A paradigm shift is taking place in medicine and dentistry from using synthetic implants and tissue grafts to a tissue engineering approach that uses degradable porous three-dimensional (3D) material hydrogels integrated with cells and bioactive factors to regenerate tissues such as dental bone and other oral tissues. Hydrogels have been establishe...
Article
Full-text available
Injectable biomaterials are increasingly being explored to minimize risks and complications associated with surgical implantation. We describe a strategy for delivery via conventional needle-syringe injection of large preformed macroporous scaffolds with well-defined properties. Injectable 3D scaffolds, in the form of elastic sponge-like matrices,...