Siam Popluechai

• PhD
• Professor (Associate) at Mae Fah Luang University

Volvariella volvacea was grown on cottonseed hull waste compost and divided into high-yield (HBE) and low-yield (LBE) conditions. Gene sequencing was used to examine bacterial and fungal populations during cottonseed husk waste composting. At the end of fermentation, the dominant bacterial genera in the HBE compost were Chelatococcus and Thermobaci...

Background Blastocystis is a unicellular eukaryote commonly found in the intestinal tract of humans and other animals. The prevalence of Blastocystis has been investigated in both developed and developing countries, yet its occurrence and distribution in rural locations has been less studied. Herein, we aimed to examine the distribution of Blastocy...

Blastocystis is a protist of controversial pathogenicity inhabiting the gut of humans and other animals. Despite a century of intense study, understanding of the epidemiology of Blastocystis remains fragmentary. Here, we aimed to explore its prevalence, stability of colonisation and association with various factors in a rural elementary school in n...

Blastocystis is a stramenopile protist of controversial pathogenicity. The organism colonizes a broad range of vertebrate and invertebrate hosts. Blastocystis has also been found in the environment both in water and soil. Several routes of transmission have been proposed including human to human, animal to human, and via contaminated food and water...

Ulcerative colitis (UC) and Crohn’s disease (CD) are two major forms of inflammatory bowel disease (IBD). The disease has been linked with gut microbiota dysbiosis in which the balance of commensal communities is disrupted. Accumulating evidence demonstrates that treatment with biologically active compounds can modulate gut microbiota composition i...

While dietary fiber has been shown to influence the composition of gut microbiota and cognitive function in adults, much less is known about the fiber-microbiome-cognition association in children. We profiled gut microbiota using quantitative PCR (qPCR) and evaluated cognitive function using the Corsi block-tapping test (CBT) and the psychomotor vi...

The Siamese fighting fish (Betta splendens Regan) is a popular ornamental fish in Thailand. Growing research suggests that fish health is influenced by gut microbiota. Here, we investigated, for the first time, the differences in the gut microbiota profiles of healthy Siamese fighting fish during the young (8-week-old) and adult male and female (16...

Blastocystis is a stramenopile protist of controversial pathogenicity. The organism colonizes a broad range of vertebrate and invertebrate hosts. Blastocystis has also been found in the environment both in water and soil. Several routes of transmission have been proposed including human-to-human, animal-to-human and via contaminated food and water....

Blastocystis is the most common protist in the gut of humans and other animals having global distribution. Occasionally, this organism has also been reported in the environment. Transmission to humans occurs via the fecal-oral route, while water also comprises a transmission route. Blastocystis has been commonly found in rivers, lakes, and wells. N...

Background Birth delivery method and breastfeeding practices contribute to microbiota colonization. Other factors including diet and demographic factors structure the gut microbiome assembly and diversity through childhood development. The exploration of these factors, especially in Southeast Asian children, remains limited. Methods We investigate...

Bee gut microbial communities have been studied extensively and linked to honey bee biology in terms of stages of bee development and behavior. Associations of bee gut microbiota in health and disease have also been explored. A large number of studies have centered on the gut microbiome of Apis mellifera, with similar investigations lagging far beh...

Blastocystis is an obligate anaerobic microbial eukaryote that frequently inhabits the gastrointestinal tract. Despite this prevalence, very little is known about the extent of its genetic diversity, pathogenicity, and interaction with the rest of the microbiome and its host. Although the organism is morphologically static, it has no less than 28 g...

Blastocystis is the most commonly found eukaryote in the gut of humans and other animals. This protist is extremely heterogeneous genetically and is classified into 28 subtypes (STs) based on the small subunit ribosomal RNA (SSU rRNA) gene. Numerous studies exist on prevalence of the organism, which usually focus on either humans or animals or the...

Introduction. Prototheca species are non-photosynthetic trebouxiophyte algae ubiquitously distributed in nature and can be found in sewage and soil. This microbial eukaryote causes human protothecosis in immunocompromised individuals. Thus, Prototheca presence in the stool of individuals without gastrointestinal symptoms has been reported only rare...

Honey bees are universally known to play a critical role in mediating pollination. Recently, attention has been drawn to the influence of gut microbiota on bee health. Studies on the honey bee (Apis mellifera) gut community have become widely appreciated due to conserved phylotypes of gut microbiota. Here, we focused on profiling the gut microbiota...

Honey bees are universally known to play a critical role in mediating pollination. Recently, attention has been drawn to the influence of gut microbiota on bee health. Studies on the honey bee (Apis mellifera) gut community have become widely appreciated due to conserved phylotypes of gut microbiota. Here, we focused on profiling the gut microbiota...

Abstract Objective This study aimed to investigate abundance of specific bacterial taxa in the saliva of 105 Thai adults with different BMI (lean, overweight, and obese) and T2DM subjects using qPCR targeting the 16S rRNA gene of various bacteria taxa. Design We employed qPCR targeting 16S rRNA genes to explore the bacterial profiles and abundance...

Obesity and type 2 diabetes mellitus (T2DM) have become major public health issues globally. Recent research indicates that intestinal microbiota play roles in metabolic disorders. Though there are numerous studies focusing on gut microbiota of health and obesity states, those are primarily focused on western countries. Comparatively only a few inv...

Background: Resistance to chemotherapeutic agents is usually found in cancer stem cells (CSCs) and cancer stem-like cells that are often regarded as the target for cancer monitoring. However, the different patterns of their transcriptomic profiling is still unclear. Objective: This study aims to illustrate the transcriptomic profile of CSCs and...

Colorectal cancer (CRC) is the third most prevalent cancer in the world. There is increasing evidence that microbial dysbiosis in the gut contributes to CRC development. However, the relationship between human microbiota and CRC is still unexplored in Thailand.

The genus Entamoeba comprises mostly gut parasites and commensals of invertebrate and vertebrate animals including humans. Herein, we report a new species of Entamoeba isolated from the gut of Asian swamp eels (Monopterus albus) in northern Thailand. Morphologically, the trophozoite is elongated and has a single prominent pseudopodium with no clear...

Gut eukaryome refers to the collection of fungi and protists in the gut. Until recently, all eukaryotic gut microbes were considered parasites and subject to elimination. Though this is true in some instances, critical evaluation of the literature reveals that most microbial eukaryotes are harmless often colonizing the human gut for long periods of...

The human gastrointestinal tract represents a collection of prokaryotic and eukaryotic microorganisms. It has been reported that gut microbiota plays a role in health and disease. Imbalance of gut microbial communities has been implicated in several non-communicable diseases including inflammatory gastrointestinal diseases, Type 2 diabetes, and obe...

Current reports suggest that obesity constitutes a serious global health issue. Emerging evidence has postulated strong links between obesity and the human gut microbiota. However, only comparatively few studies come from Asia and consequently the gut microbiota of lean and obese adult Asians remain largely unexplored. Herein, we sought to investig...

Blastocystis is a common and broadly distributed microbial eukaryote inhabiting the gut of humans and other animals. The genetic diversity of Blastocystis is extremely high comprising no less than 17 subtypes in mammals and birds. Nonetheless, little is known about the prevalence and distribution of Blastocystis subtypes colonising humans in Thaila...

Loss of traditional diets by food globalization may have adverse impact on the health of human being through the alteration of gut microbial ecosystem. To address this notion, we compared the gut microbiota of urban (n = 17) and rural (n = 28) school-aged children in Thailand in association with their dietary habits. Dietary records indicated that...

Jatropha seeds can be used to produce high-quality biodiesel due to their high oil content. However, Jatropha produces low numbers of female flowers, which limits seed yield. Paclobutrazol (PCB), a plant growth retardant, can increase number of Jatropha female flowers and seed yield. However, the underlying mechanisms of flower development after PC...

Streptococcus spp. are facultative anaerobic bacteria, which are dominant through the gastrointestinal tract. This genus has been found to associate with both oral and intestinal diseases, whereas some Streptococcus spp. were found with beneficial abilities against the streptococcal pathogens. The result of these interactions may become a factor ba...

To develop qPCR assay for Streptococcus spp. absolute quantification in salivary and fecal DNA extracts obtained from different BMI volunteers.

In this study, bacterial biocontrol agents against the phytopathogen Lasiodiplodia theobromae were screened from Thua Nao, a Thai fermented soybean product. A total of 170 bacterial strains were isolated and initially screened for their antagonistic activity by dual culture test. Of these, 39 isolates were able to inhibit the fungal growth showing...

Background: Luem-Pua glutinous rice has been reported to contain a high content of resistant starch (RS). Previous studies showed weight loss, decreased blood glucose and lipid profiles levels, as well as decreased hyperplasia and hypertrophy of adipocytes in RS treatment. However, the study of protective effects of RS isolated from Luem-Pua glutin...

Jatropha curcas L. has been considered as a potential source of biodiesel feedstock due to its highly seed oil content. However, Jatropha exhibits low numbers of female flowers leading to insufficient seed yields for biodiesel production. Previous studies showed that application of 6-benzyladenine (BA) on Jatropha flower buds significantly increase...

The burden of cancer death has increased drastically in the past few decades; one of the leading causes of death is colorectal cancer. Diet and gut microbiota play an important role in colorectal cancer carcinogenesis. High fiber-diet consumption has been suggested a cancer preventive effect.

Jatropha curcas L. has been considered a potential source of biodiesel feedstock due to its high seed oil content. However, Jatropha exhibits low numbers of female flowers leading to insufficient seed yields for biodiesel production. The application of paclobutrazol (PCB) on various types of plants, including Jatropha, has been reported to increase...

Jatropha curcas L. (Jatropha) is a potential plant whose seeds could be used for biofuel production from their seeds. However, Jatropha plants have less number of female flowers than number of male flowers which affects to lower seed yield. The application of plant growth retardant such as Paclobutrazol (PCB) can be used for increasing plant produc...

The burden of cancer death has increased drastically in the past few decades; one of the leading causes of death is colorectal cancer. Diet and gut microbiota play an important role in colorectal cancer carcinogenesis. High fiber-diet consumption has been suggested a cancer preventive effect.

The human gastrointestinal tract harbors a complex community consisting of numerous bacterial taxa. Investigating presence and abundance of a taxon of interest in the community requires specific primers and quantitative PCR (qPCR) assay. In this study, 16S rRNA gene-targeted streptococcal genus-specific primers were designed and validated for speci...

Latex proteins have been previously characterized in various plants including their indispensable roles. However, latex protein profiles are not well defined in Jatropha, neither of toxic nor non-toxic varieties. To compare the protein profiles between toxic and non-toxic latex we employed a proteomic approach using one-dimensional gel electrophore...

The expression of Gibberellin (GA) biosynthetic genes is of particular importance in promoting plant growth and development. Gibberellin inhibitors such as Paclobutrazol (PCB), interrupts ent-kaurene oxidase (KO) enzyme activity in the GA biosynthetic pathway which reduces plant height. Nonetheless, GA inhibitors may benefit harvesting and seed yie...

Fungal endophytes are microorganisms that are well-known for producing a diverse array of secondary metabolites. Recent studies have uncovered the bioprospecting potential of several plant endophytic fungi. Here, we demonstrate the presence of highly bioactive fungal endophytic species in Aquilaria subintegra, a fragrant wood plant collected from T...

Butyrate is a short chain fatty acids often produced in the colonic lumen by the bacterial fermentation of dietary fiber and resistant starch. Butyrate has been shown to induce cell cycle arrest at the G1 phase, cell differentiation, and apoptosis of cancer cells including colorectal cancer (CRC). Recently, several studies has been proposed that on...

One of environmental stresses that plants are often subjected to is wounding stress. In order to investigate the defense response of LRR (leucine-rich repeat) gene upon wounding stress, mechanical wounding on Jatropha leaves was conducted and the gene expression was analyzed by quantitative PCR (qPCR). Quantitative PCR results demonstrated distinct...

Jatropha curcas L. (Jatropha) produce seed oil which is interested in having potential as an economical alternative biodiesel fuel source. Seed yield is the important factor which effects directly to oil production. However, Jatropha is produced low seed yield resulting in insufficient for biodiesel production. Paclobutrazol (PCB) is one of plant g...

Cultivated rice (Oryza sativa L.) is genetically diverse, and the numerous landraces represent a valuable genetic resource for present and future rice breeding. Part of the genetic rice diversity is held by the ethnic minority groups of Tai Lue and Akha who migrated from southern China to northern Thailand over the past two centuries. We analysed v...

Jatropha curcas L. (Jatropha) is widely interested as the alternative fuel sources. However, Jatropha produces phorbol esters (PEs) which are considered as its major toxic compounds. Two varieties were classified based on the presence of PEs, i.e. toxic and non-toxic varieties. In this study, expression profiles of the genes that might be involved...

Jatropha curcas L. (Jatropha) produces seed oil, a potential source for biodiesel production. Seed yield of Jatropha is an important factor for biodiesel production, despite few flower number productions leading to low seed yield production. This study attempted to identify floral transcription factor genes using bioinformatics approach, which have...

Human gut microbiota are diverse and different among individuals. It has been reported that composition of gut microbiota related to the host physiological conditions and diseases such as obesity, diabetes.Therefore, our study aimed to characterize the composition of gut microbiota including Bacteroidetes, Firmicutes, Proteobacteria, and Actinobact...

Ninety percent of the world's energy demands are met by non-renewable fossil fuels. The limited supply, high prices and non-sustainability of fossil fuels has led to exploring new resources for energy. Among all the available sources of renewable energy, biodiesel is viewed as one of the best alternatives, particularly for transport fuel, because i...

The volatile components of Green Oolong tea No. 12 fermented with culture supernatants of five Bacillus subtilis strains were investigated. Initially, the culture supernatants of five different strains of B. subtilis were prepared and subsequently used as crude enzymes to ferment tea samples. After 2 h-fermentation, the volatile components were ext...

Jatropha curcas has been recognized as a potential source for biodiesel production. However, the plants contains phorbol esters which are carcinogenic. Phorbol esters are members of diterpenes group. In this study, accumulations of mRNA of five genes involved in diterpene biosynthesis; isopentenyl diphosphate/dimethylallyl diphosphate synthase (IDD...

The seed oil of Jatropha curcas has been proposed as a source of biodiesel. In plants, seed oil is stored in subcellular organelles called oil bodies (OBs), which are stabilized by proteins. Proteome composition of the J. curcas OBs revealed oleosins as the major component and additional proteins similar to those in other oil seed plants. Three J....

This book, containing 20 chapters, addresses the major concerns of scientists, policy makers, environmental lobby groups and the general public regarding the controversial issue on environmental impact (e.g. on soil and water ecology and nontarget organisms) of transgenic crops, from an editorially neutral standpoint. While the main focus is on env...

Due to the increasing popularity of Jatropha curcas as a feedstock for biodiesel, generating non-toxic and high yielding varieties of the plant requires genotypic characterization towards identifying breeding lines. There is little information on the phylogenetic relationships between its global accessions and species. Assessing genetic variation b...

Catechins are a group of polyphenols found in tea (Camellia sinensis var. sinensis) at high levels. They are beneficial for health. From the study on accumulation of catechins in shoots and mature leaves of a tea cultivar, Oolong No. 17, using high-performance liquid chromatography (HPLC), it was found that the amounts of most catechins in the shoo...

In this study, the phylogenetic relationships among twelve lines of Cucumis sativus were studied using Randomly Amplified Polymorphic DNA (RAPD) markers. A modified CTAB DNA extraction protocol was introduced to obtain genomic DNA from young leaves. Of forty random primers tested, only fifteen primers were able to successfully amplify all genomic D...

RAPD analysis of nine Thai pineapple cultivars, including 'Phulae', 'Sawee', 'Tradsithong', 'Phuket', 'Pattavia', 'Intrachitdang', 'Intrachitkhow', 'Petburi No.1', and 'Nanglae', showed that, of 40 arbitrary 10- mer primers, 17 primers gave 206 DNA fragments ranging from 510 to 4,700 bp. One hundred and forty-five (70.4%) of the amplified fragments...

Total polyphenols (TP) in some Chiang Rai leaf tea products were analysed by spectrophotometric method based on Folin-Ciocalteau's reaction. The TP contents in green, oolong and black tea products, produced at different degree of fermentation, were compared. The leaf tea samples included 12 samples of green tea, 11 samples of oolong tea, and 4 samp...