Shumaila Naz

Shumaila Naz
University of Wah · Department of Biosciences

PhD
Chairperson Department of Biosciences University of Wah

About

26
Publications
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111
Citations

Publications

Publications (26)
Article
Full-text available
Objective: To determine the patterns of resistance among different macrolides and to detect macrolide resistance genes in Staphylococcus aureus collected from different clinical samples. Materials and Methods: One hundred non-repetitive clinical isolates were obtained from different clinical samples (pus, urine, sputum and blood). Each Staphylococc...
Article
Full-text available
Objectives The current study was conducted to evaluate the antibacterial potential of leaf and fruit extracts of Zanthoxylum armatum against two pathogenic bacterial isolates, Staphylococcus aureus and Staphylococcus epidermidis. Methods Twelve commercially available antibiotics were tested S. aureus and S. epidermidis by antimicrobial susceptibil...
Article
Full-text available
Epialleles that emerge due to methylation variation in genetically identical individuals are gaining more interest due to their involvement in physiological and pathological processes. These are also important for transgenerational epigenetic inheritance and evolution. Both stable and metastable epialleles have their importance because of their con...
Article
Full-text available
Massive discharge of wastes produced by the processing of leather so far confers the most important environmental challenge facing the tanneries worldwide. Waste material from tanneries mostly consists of skin remnants and proteinaceous substances as by-products of leather processing. In these conditions, protease-producing bacteria play a vital ro...
Article
The present study evaluates the effects of plant growth hormones (PGR), salicylic acid (SA), abscisic acid (ABA) and plant growth promoting rhizobacteria (PGPRs) Rhizobium pisi (DSM 30132 strain) applied alone and in combination, on pea (Pisum sativum L.) cv. Florida plants under well-watered and drought stressed conditions. Prior to sowing seeds w...
Article
Full-text available
Increased demand of enzymes for industrial use has led the scientists towards protein engineering techniques. In different protein engineering strategies, rational approach has emerged as the most efficient method utilizing bioinformatics tools to produce enzymes with desired reaction kinetics; physiochemical (temperature, pH, half life, etc) and b...
Article
Full-text available
Wounds, burns, cuts, and scarring may cause a serious problem for human health if left untreated, and medicinal plants are identified as potentially useful for wound healing. Therefore, the study focused on ethnophytotherapy practices for wound healing from an unexplored area, Pakistan. Ethnophytotherapeutic information was collected through well-p...
Article
Increased demand of enzymes for industrial use has led the scientists towards protein engineering techniques. In different protein engineering strategies, rational approach has emerged as the most efficient method utilizing bioinformatics tools to produce enzymes with desired reaction kinetics; physiochemical (temperature, pH, half life, etc) and b...
Article
Increased demand of enzymes for industrial use has led the scientists towards protein engineering techniques. In different protein engineering strategies, rational approach has emerged as the most efficient method utilizing bioinformatics tools to produce enzymes with desired reaction kinetics; physiochemical (temperature, pH, half life, etc) and b...
Article
Full-text available
Background Recombinant DNA technology enables us to produce proteins with desired properties and insubstantial amount for industrial applications. Endo-1, 4-β-glucanases (Egl) is one of the major enzyme involved in degradation of cellulose, an important component of plant cell wall. The present study was aimed at enhancing the production of endo-1,...
Article
Full-text available
Urinary Tract Infection (UTI) is one of the most common infectious disease, ranking next to upper respiratory tract infection in their incidence and a foremost cause of morbidity and mortality in human. The study aimed to determine the antibiotic resistance patterns of the most common bacteria, isolated from nosocomial urinary tract infections (i.e...
Conference Paper
Full-text available
Monte Carlo (MC) methods are computational algorithms that rely on repeated random sampling to obtain numerical results. In principle, these methods can be used to solve any problem having a probabilistic interpretation. In biology, MC simulations are frequently used to model propagation of light in tissue. The model can be built so that increasing...
Article
Full-text available
ABSTRACT The objective of current study was to determine the frequency of microorganism causing UTIs in pregnant women and antimicrobial susceptibility of UTIs causing microorganism against Cefexime, Imipenem and Carbenicillin. Total 63 samples were collected and cultured on CLED agar medium. The positive samples were identified by characteristic a...
Article
Full-text available
A putative β-glucosidase gene from the genome of Bacillus halodurans C-125 was expressed in E. coli under the regulation of T7lac promoter. On induction with isopropyl-β-D-1-thiogalactopyranoside, the enzyme expressed at ∼40% of the cell protein producing 238 mg/liter culture. With increase in culture cell density to A 600 12 in auto-inducing M9NG...
Article
Full-text available
A subtilisin gene identified in the reported genome sequence of Pyrococcus furiosus was amplified and inserted in pET-22b(+) vector to produce the recombinant plasmid pET-SB. Escherichia coli BL-21 (DE3) CodonPlus was transformed with this plasmid and the enzyme was expressed up to 30% of the total cell protein on induction with IPTG. The expressed...
Article
Full-text available
A subtilisin gene identified in the reported genome sequence of Pyrococcus furiosus was amplified and inserted in pET-22b(+) vector to produce the recombinant plasmid pET-SB. Escherichia coli BL-21 (DE3) CodonPlus was transformed with this plasmid and the enzyme was expressed up to 30% of the total cell protein on induction with IPTG. The expressed...

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