Shuke Wu

Shuke Wu
Huazhong Agricultural University | HZAU · College of Life Science and Technology

PhD
Looking for PhD students, Postdocs, and Co-PIs in Biocatalysis/Enzyme Engineering/Microbe Engineering~

About

39
Publications
9,675
Reads
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1,681
Citations
Citations since 2016
32 Research Items
1623 Citations
20162017201820192020202120220100200300400500
20162017201820192020202120220100200300400500
20162017201820192020202120220100200300400500
20162017201820192020202120220100200300400500

Publications

Publications (39)
Article
Full-text available
New types of asymmetric functionalizations of alkenes are highly desirable for chemical synthesis. Here, we develop three novel types of regio- and enantioselective multiple oxy- and amino-functionalizations of terminal alkenes via cascade biocatalysis to produce chiral α-hydroxy acids, 1,2-amino alcohols, and α-amino acids, respectively. Basic enz...
Article
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The biotin-streptavidin technology has been extensively exploited to engineer artificial metalloenzymes (ArMs) that catalyze a dozen different reactions. Despite its versatility, the homotetrameric nature of streptavidin (Sav) and the non-cooperative binding of biotinylated cofactors, impose two limitations on the genetic optimization of ArMs: i) p...
Article
Full-text available
Engineered enzyme cascades offer powerful tools to convert renewable resources into value-added products. Man-made catalysts give access to new-to-nature reactivities that may complement the enzyme’s repertoire. Their mutual incompatibility, however, challenges their integration into concurrent chemo-enzymatic cascades. Herein we show that compartm...
Article
Full-text available
Biocatalysis has found numerous applications in various fields as an alternative to chemical catalysis. The use of enzymes in organic synthesis, especially to make chiral compounds for pharmaceuticals as well for the flavors and fragrance industry, are the most prominent examples. In addition, biocatalysts are also used on large scale to make speci...
Article
Full-text available
Fast screening of enzyme variants is crucial for tailoring biocatalysts for the asymmetric synthesis of non-natural chiral chemicals, such as amines. However, most existing screening methods either are limited by the throughput or require specialized equipment. Herein, we report a simple, high-throughput, low-equipment dependent, and generally appl...
Article
Full-text available
This work reports a new strategy for the detection and purification of levulinic acid (LA) and bio-compounds from the acid hydrolysis and enzymatic treatment of oil palm empty fruit bunch (OPEFB) through high-performance liquid chromatography (HPLC) techniques combined with ion/ligand chromatography. The detections of LA, biomass-saccharides, hydro...
Article
Synthetic biology, a course with a sound theoretical system and a wide application range, plays a role in the cultivation of innovative talents in the field of bioengineering. To this end, we have set up a synthetic biology course in our university. First, according to the concept of imparting basic knowledge, highlighting innovative practice, and...
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Plastic waste imposes a serious problem to the environment and society. Hence, strategies for a circular plastic economy are demanded. One strategy is the engineering of polyester hydrolases towards higher activity for the biotechnological recycling of polyethylene terephthalate (PET). To provide tools for the rapid characterization of PET hydrolas...
Article
While the typical targets of (chemo-)enzymatic cascades are fine chemicals (e.g., pharmaceuticals), a chemoenzymatic cascade, artificial starch anabolic pathway (ASAP), was recently developed to synthesize starch from CO2. The key results and outstanding features of ASAP are discussed here. We envision that ASAP and its microbial counterpart may en...
Article
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Developing (chemo)enzymatic cascades is very attractive for green synthesis, because they streamline multistep synthetic processes. In this Feature Article, we have summarized the recent advances in in vitro or whole-cell cascade reactions with a focus on the use of renewable bio-based resources as starting materials. This includes the synthesis of...
Article
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Biocatalysis has undergone revolutionary progress in the past century. Benefited by the integration of multidisciplinary technologies, natural enzymatic reactions are constantly being explored. Protein engineering gives birth to robust biocatalysts that are widely used in industrial production. These research achievements have gradually constructed...
Article
Apremilast is an important active pharmaceutical ingredient that relies on a resolution to produce the key chiral amine intermediate. To provide a new catalytic and enzymatic process for Apremilast, we performed the directed evolution of the amine transaminase from Vibrio fluvialis. Six rounds of evolution resulted in the VF-8M-E variant with >400-...
Article
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Biocatalytic alkylations are important reactions to obtain chemo‐, regio‐ and stereoselectively alkylated compounds. This can be achieved using S‐adenosyl‐l‐methionine (SAM)‐dependent methyltransferases and SAM analogs. It was recently shown that a halide methyltransferase (HMT) from Chloracidobacterium thermophilum can synthesize SAM from SAH and...
Article
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Biokatalytische Alkylierungen sind wertvolle Funktionalisierungen im späten Synthesestadium, erfordern aber S‐Adenosylmethionin (SAM)‐Analoga. Mithilfe gerichteter Evolution wurde eine Halogenid‐Methyltransferase erzeugt, die preiswerte und gut verfügbare Alkyliodide in SAM‐Analoga umsetzen kann. In einer Kaskade mit Methyltransferasen ermöglichte...
Article
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Wir berichten über das Protein‐Engineering der P450‐Monooxygenase CYP107D1 für die stereo‐ und regioselektive 7β‐Hydroxylierung von Lithocholsäure (LCS) zur Herstellung von Ursodeoxycholsäure (UDCS). Selektivitätsbeeinflussende Reste wurden identifiziert und eine „small but smart“ Mutantenbibliothek erstellt. Durch einen farbbasierten Schnelltest k...
Article
Full-text available
We engineered the cytochrome P450 monooxygenase CYP107D1 (OleP) from Streptomyces antibioticus for the stereo-and regioselective 7β-hydroxylation of lithocholic acid (LCA) to yield ursodeoxycholic acid (UDCA). OleP was previously shown to hydroxylate testosterone at the 7β-position but LCA is exclusively hydroxylated at the 6β-position, forming mur...
Article
Full-text available
Biocatalysis has found numerous applications in various fields as an alternative to chemical catalysis. The use of enzymes in organic synthesis, especially to make chiral compounds for pharmaceuticals as well for the flavors and fragrance industry, are the most prominent examples. In addition, biocatalysts are also used on large scale to make speci...
Article
As an important bulk chemical, benzoic acid is currently manufactured from non‐renewable feedstocks under harsh conditions. Although there are natural pathways for biosynthesis of benzoic acid, they are often inefficient and subjected to complex regulation. Here we develop a non‐natural enzyme cascade to efficiently produce benzoic acid from styren...
Article
(S)‐Mandelic acid is a useful and high value chemical with many synthetic applications, but its synthesis often requires the use of toxic cyanide. Here, we report the development of several cyanide‐free methods to prepare (S)‐mandelic acid via cascade biotransformation. Enhanced production of (S)‐mandelic acid from styrene via 4‐step enzyme cascade...
Article
Alkenes are excellent starting materials for organic synthesis due to the versatile reactivity of C=C bonds and the easy availability of many unfunctionalised alkenes. Direct regio- and/or enantioselective conversion of alkenes into functionalised (chiral) compounds has enormous potential for industrial applications, and thus attracts extensive dev...
Article
2‐phenylethanol (2‐PE) is an aroma compound with a rose‐like odors widely used as food ingredient, and natural 2‐PE is much preferred for the application with high selling price but limited availability. Bioproduction of 2‐PE from bioresources could provide a green, sustainable, and economic process to manufacture natural 2‐PE. Here we report a nov...
Article
Production of chemicals from renewable feedstocks has been an important task for sustainable chemical industry. While microbial fermentation has been widely employed to produce many biochemicals, it is still very challenging to access non‐natural chemicals. Herein, we developed two methods (biotransformation and fermentation) for the first bio‐deri...
Article
Enzymatic alcohol oxidation plays an important role in chemical synthesis. In the past two years, new alcohol oxidation enzymes were developed through genome-mining and protein engineering, such as new copper radical oxidases with broad substrate scope, alcohol dehydrogenases with altered cofactor preference and a flavin-dependent alcohol oxidase w...
Article
Performing one-pot multi-catalysis reactions is a revolutionary tool for multistep synthesis, representing a fast-developing theme in catalysis field. To develop cascade reactions, enzymes are ideal catalysts due to their compatible reaction conditions. The implementation of enzyme cascades in recombinant microbial cells provides easily available s...
Article
Enantiopure ᴅ-phenylglycine and its derivatives are an important group of chiral amino acids with broad applications in pharmaceutical industry. However, the existing synthetic methods for ᴅ-phenylglycine mainly rely on toxic cyanide chemistry and multistep processes. To provide green and safe alternatives, we leveraged cascade biocatalysis for one...
Article
Biocatalytic anti-Markovnikov alkene hydroamination and hydration were achieved based on two concepts involving enzyme cascades: epoxidation-isomerization-amination for hydroamination and epoxidation-isomerization-reduction for hydration. An Escherichia coli strain co-expressing styrene monooxygenase (SMO), styrene oxide isomerase (SOI), ω-transami...
Article
Green and selective oxidation methods are highly desired in chemical synthesis and manufacturing. In this work, we have developed a biocatalytic method for the regio- and stereoselective oxidation of styrene derivatives into arylacetic and (S)-2-arylpropionic acids via a one-pot epoxidation–isomerization–oxidation sequence. This was done via the en...
Article
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Biotransformation is a green and useful tool for sustainable and selective chemical synthesis. However, it often suffers from the toxicity and inhibition from organic substrates or products. Here, we established a hollow fiber membrane bioreactor (HFMB)-based aqueous/organic biphasic system, for the first time, to enhance the productivity of a casc...
Article
Oxidative cascade biocatalysis enabling multistep enzyme-catalyzed reactions in one pot and utilizing molecular oxygen as a green and cheap oxidant is an emerging tool for selective and sustainable chemical synthesis. In this Account, we summarize several new types of oxidative cascade biocatalysis for the regioselective and mostly enantio­selectiv...
Article
Sustainable synthesis of useful and valuable chiral fine chemicals from renewable feedstocks is highly desirable but remains challenging. Reported herein is a designed and engineered set of unique non-natural biocatalytic cascades to achieve the asymmetric synthesis of chiral epoxide, diols, hydroxy acid, and amino acid in high yield and with excel...
Article
Sustainable synthesis of useful and valuable chiral fine chemicals from renewable feedstocks is highly desirable but remains challenging. Reported herein is a designed and engineered set of unique non-natural biocatalytic cascades to achieve the asymmetric synthesis of chiral epoxide, diols, hydroxy acid, and amino acid in high yield and with excel...
Article
A new type of cascade biocatalysis was developed for one-pot enantioselective conversion of a meso- or racemic epoxide to an α-hydroxy ketone in high ee via an epoxide hydrolase-catalyzed hydrolysis of the epoxide, an alcohol dehydrogenase-catalyzed oxidation of the diol intermediate, and an enzyme-catalyzed cofactor regeneration. In vitro cascade...
Article
P450tol monooxygenase was discovered as a unique and highly enantioselective enzyme for asymmetric epoxidation of some terminal alkenes containing electron-withdrawing groups and benzylic hydroxylation of several ethylbenzenes giving the corresponding useful and valuable products, such as (R)-2- and 3-substituted styrene oxides, (S)-4-substituted s...
Article
Full-text available
Synthetic genetic circuits are programmed in living cells to perform predetermined cellular functions. However, designing higher-order genetic circuits for sophisticated cellular activities remains a substantial challenge. Here we program a genetic circuit that executes Pavlovian-like conditioning, an archetypical sequential-logic function, in Esch...
Article
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A novel concept integrating supramolecular self-assembly and electrostatic complexation at an aqueous liquid–liquid interface to synthesize stable peptide–polymer hybrid capsules was developed. The concept was further applied for enzyme immobilization to give stable and active biocatalysts with low enzyme leakage and high encapsulation efficiency,...
Article
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Cascade biocatalysis via intracellular epoxidation and hydrolysis was developed as a green and efficient method for enantioselective dihydroxylation of aryl olefins to prepare chiral vicinal diols in high ee and high yield. Escherichia coli (SSP1) coexpressing styrene monooxygenase (SMO) and epoxide hydrolase SpEH was developed as a simple and effi...
Article
Full-text available
A unique epoxide hydrolase (SpEH) from Sphingomonas sp. HXN-200 was identified and cloned based on genome sequencing and expressed in Escherichia coli. The engineered E. coli (SpEH) showed the same selectivity and substrate specificity as the wild type strain and 172 times higher activity than Sphingomonas sp. HXN-200 for the hydrolysis of styrene...
Article
Aimed at bioremediation of the soil contaminated by a coking plant, polycyclic aromatic hydrocarbons (PAHs)- degrading bacteria were isolated from the soil and their degrading properties were studied. Streak-plating method was applied to isolate the PAHs-degrading bacteria using fluorine (3 rings), fluoranthene (4 rings) and benzo[b]-fluoranthene (...

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