Shubhagata Das

Charles Sturt University · School of Animal and Veterinary Sciences

Rotavirus A is a leading cause of non-bacterial gastroenteritis in humans and domesticated animals. Despite the vast diversity of bovine Rotavirus A strains documented in South Asian countries, there are very few whole genomes available for phylogenetic study. A cross-sectional study identified a high prevalence of the G6P[11] genotype of bovine Ro...

We report the first detection and prevalence of Beak and feather disease virus (BFDV) in Australia’s Red Goshawk (Erythrotriorchis radiatus). This is a new host for this pervasive pathogen amongst a growing list of non-psittacine species including birds of prey from the orders Accipitriformes (hawks, eagles, kites), Falconiformes (falcons and carac...

Canine circovirus (CanineCV) is an emerging pathogen in domestic dogs, detected in multiple countries in association with varying clinical and pathological presentations including diarrhoea, vasculitis, granulomatous inflammation, and respiratory signs. Understanding the pathology of CanineCV is confounded by the fact that it has been detected in a...

The emergence of antimicrobial resistance in commensal bacteria poses a serious public health burden worldwide. Commensals can disseminate the resistance genes to pathogenic bacteria causing life-threatening infections. This cross-sectional study was designed to investigate the antimicrobial resistance pattern and molecular mechanism(s) of ciproflo...

Pigeon circovirus (PiCV) is considered to be genetically diverse, with a relatively small circular single-stranded DNA genome of 2 kb that encodes for a capsid protein (Cap) and a replication initiator protein (Rep). Australasia is known to be the origin of diverse species of the Order Columbiformes, but limited data on the PiCV genome sequence has...

Simple Summary Pets may die unexpectedly, without warning, and with no externally visible reason for death. As in people, postmortem examination can be performed to try to determine the cause of death. There are many diseases which may result in sudden unexpected death in domestic animals, although there is little research on companion animals and...

Septic synovitis and peritonitis are routinely diagnosed in horses based on clinical examination findings and laboratory assessment of synoviocentesis and abdominocentesis samples, respectively. Diagnosis is difficult in some cases because of an overlap in laboratory results for septic and non-septic inflammation. Neutrophil extracellular trap (NET...

Columbid alphaherpesvirus 1 (CoHV1) is associated with oral or upper respiratory tract lesions, encephalitis, and occasional fatal systemic disease in naive or immunosuppressed pigeons. Clinical disease is often reported with CoHV1 and coinfecting viruses, including pigeon circovirus (PiCV), which may cause host immunosuppression and augment lesion...

Columbid alphaherpesvirus 1 (CoHV-1) is a well-recognised viral pathogen in pigeons with a global distribution. Infected birds may remain asymptomatic carriers with episodic recurrence and shedding in endemically infected flocks. When introduced to naïve or immunosuppressed population it may cause oral or upper respiratory tract pathology, encephal...

E. coli and Salmonella spp. are responsible for causing colibacillosis and salmonellosis in chickens respectively. This research work was undertaken to study the prevalence of colibacillosis and salmonellosis in commercial chickens of Chattogram, and to know the antibiogram profiles of the isolated bacteria. A well-structured questionnaire was used...

TaqMan probe based quantitative polymerase reaction (TaqMan qPCR) is a robust and reliable technique for detecting and quantifying target DNA copies. Quantitative molecular diagnosis of genetically diverse single stranded DNA (ssDNA) virus such as Pigeon circovirus (PiCV) can be challenging owing to difficulties in primer binding or low abundance o...

Canine parvovirus 2 (CPV-2) outbreaks in close quarters such as kennels or shelters can cause substantial case fatality. Thirteen dead Labradors from a secluded kennel of security dogs presented with typical clinical signs and gross pathology of parvovirus infection. Whole genome shotgun sequencing from tissue-extracted genomic DNA detected new CPV...

Bacterial disease of poultry is one of the major constraints to the expansion of poultry industry. The study was undertaken to investigate some common bacterial diseases in commercial poultry farm. A total of 100 sick and dead chickens (67 broilers, 26 layers and 7 sonali) were collected from different poultry farms which were subjected to postmort...

Background Lumpy skin disease (LSD) is an important viral disease causing significant economic losses in commercial livestock production. In mid‐2019, an outbreak of LSD has been reported in cattle population from different parts of Bangladesh including Chattogram division. A cross‐sectional surveillance study was undertaken from August 2019 to Dec...

Dog bite is one of the most common and significant predisposing factors for transmission of rabies from reservoir to other hosts including human. Human perception about infectious diseases like rabies is arguably the most deciding factor in controlling epidemics. A cross sectional study was conducted on dog bite cases in domestic and pet animals of...

Circoviruses represent a rapidly expanding group of viruses that infect both vertebrate and invertebrate hosts. Members are responsible for diseases of veterinary and economic importance, including postweaning multisystemic wasting syndrome in pigs, and beak and feather disease (BFD) in birds. These viruses are associated with lymphoid depletion an...

Conservation efforts for the orange-bellied parrot ( Neophema chrysogaster), one of the world's most critically endangered bird species, have been hampered by beak and feather disease virus (BFDV) spillover infection. To understand the vulnerability of orange-bellied parrots to potential reservoirs of infection we investigated geographic versus tax...

The establishment of viral pathogens in new host environments following spillover events probably requires adaptive changes within both the new host and pathogen. After many generations, signals for ancient cross-species transmission may become lost and a strictly host-adapted phylogeny may mimic true co-divergence while the virus may retain an inh...

Avipoxviruses are significant pathogens infecting a wide range of wild and domestic bird species globally. Here, we describe a novel genome sequence of magpiepox virus (MPPV) isolated from an Australian magpie. In the present study, histopathologically confirmed cutaneous pox lesions were used for transmission electron microscopic analysis, which d...

Trichomonas is a significant protist genus, and includes T. vaginalis, the most prevalent sexually transmitted non-viral infection of humans, and T. gallinae of rock doves (Columba livia), one of the earliest known avian pathogens. New Trichomonas genotypes, including T. vaginalis-like isolates, have been discovered in American columbid hosts, sugg...

This poster was presented at 16th International Scientific Conference 2019 of Chattogram veterinary and Animal Sciences University. It was awarded as the best poster of the conference.

This study was aimed to sequence the first complete mitochondrial genome from an Indian ringneck parrot (Psittacula krameri). The mitogenome sequence was circular and 16,413 bp in length. In comparison to other available mitogenome sequences belonging to Psittacidae species, this mitogenome encoded a conserved structure consisting of 13 protein-cod...

Like other avian circovirus species Pigeon circovirus (PiCV) is known to be genetically diverse with a relatively small circular single stranded DNA genome of 2 kb that encodes for a capsid protein (Cap) and a replication initiator protein (Rep). Recent paleoviral evidence hints towards a probable Gondwanan origin of avian circoviruses, paralleling...

In this paper, we report the genomic architecture of a complete mitogenome from a Wedge-tailed eagle (Aquila audax). The mitogenome sequence was circular, and 17,494 bp in length. Compared to other members of the family Accipitridae, the genome encoded a conserved structure consisting of 13 protein-coding genes (PCGs), two rRNA genes, 22 tRNA genes...

The complete mitochondrial genome of Indian star tortoise (Geochelone elegans) was characterized having 16,446 bp nucleotides encoding 37 genes in circular orientation comprising 13 protein-coding genes, 22 tRNA genes and two rRNA genes. The lengths of 12S and 16S ribosomal RNA were 973 bp and 1600 bp. A non-coding control region (D-Loop) of 966 bp...

Bovine rotavirus A is a leading cause of diarrhea in calves. Diarrhea causes excessive water and electrolyte loss through the gastroin-testinal (GI) tract and subsequently produces dehydration, and in acute cases can lead to death. The aim of the study was to evaluate haemato-biochemical changes occurred in diarrheic calves as well as rotavirus aff...

Objective: Salmonella is a widely-reported zoonotic bacterial pathogen and human infection is mostly attributed through direct or indirect contact with chickens. Salmonella Kentucky (S. Kentucky) is one of the motile serovars which has recently been identified from both poultry and human samples in Bangladesh. This study was conducted to assess its...

This paper describes the genomic architecture of a complete mitogenome from a red-bellied parrot (Poicephalus rufiventris). The mitogenome sequence was circular and 15,524 bp in length. Compared to other Psittacidae species, the genome encoded a conserved structure consisting of 13 protein-coding genes (PCGs), two rRNA genes, 21 tRNA genes, and two...

Objective: Characterisation of a complete genome sequence of an Australian strain of canid alphaherpesvirus 1 (CHV-1) and its phylogenetic relationship with other varicellovirus species. Methods: Standard pathology and PCR methods were used to initially detect herpesvirus in hepatic tissue from an infected 4-week-old Labrador Retriever puppy. Th...

Background: This paper describes the pathology associated with psittacine beak and feather disease in a wild sulphur-crested cockatoo with concurrent knemidocoptic mange, cestodiasis and mycotic encephalitis. Methods & results: Large numbers of Knemidocoptes pilae Lavoipierre and Griffiths, 1951 (Acari: Epidermoptidae, Knemidokoptinae) were iden...

In this paper, we report the complete mitochondrial genome of an Australian little penguin (Eudyptula minor novaehollandia). The mitogenome sequence has been found to be circular, and 17,608 bp in length. Similar to other Spheniscidae species, the genome encoded a typically conserved structure consisting of 13 protein-coding genes (PCGs), two rRNA...

Background Over the past 20 years, many marine seabird populations have been gradually declining and the factors driving this ongoing deterioration are not always well understood. Avipoxvirus infections have been found in a wide range of bird species worldwide, however, very little is known about the disease ecology of avian poxviruses in seabirds....

The complete genome sequence of beak and feather disease virus (BFDV) was discovered from a rainbow bee-eater ( Merops ornatus ), a species of Coraciiformes . The genome consisted of 1,996 bp encoding two major bidirectional transcribed open reading frames. This is the first evidence of BFDV infection and complete genome characterization for this n...

The complete genome sequence of beak and feather disease virus (BFDV) from a fledgling red-capped parrot ( Purpureicephalus spurius ) was assembled and characterized. The genome consists of 1,995 nucleotides and encodes two major proteins in opposing directions. This is the first evidence of BFDV infectivity and a complete genome sequence for this...

Three complete genomes of beak and feather disease virus (BFDV) were recovered from wild musk lorikeets (Glossopsitta con-cinna). The genomes consisted of 2,008 to 2,010 nucleotides (nt) and encode two major proteins transcribing in opposing directions. This is the first report of BFDV complete genome sequences obtained from this host species.

The complete genome sequence of beak and feather disease virus (BFDV) from a purple crowned lorikeet (Glossopsitta porphy-rocephala) was characterized. The genome consists of 2,010 nucleotides and encodes replicase-associated protein and capsid protein. This is the first evidence of BFDV infectivity and complete genome sequence for this novel host.

In this paper, the complete mitochondrial genome of an Endangered Ninox strenua is sequenced and reported for the first time. The mitogenome of N. strenua is a circular molecule of 16,206 bp in length, consisting of 13 protein-coding genes (PCGs), 22 tRNA, 2 rRNA, and a control region (D-loop). All the genes in N. strenua are distributed on the H-s...

p>The present study was the first postmortem examination of a barn owl in Bangladesh. A female Barn owl weighing 450grams was brought to the Teaching Veterinary Hospital (TVH) at Chittagong Veterinary and Animal Sciences University (CVASU) by the forest department, Chittagong. The history of traumatic injury on left wing by an electric fan one day...

This study was carried out to isolate the infectious bronchitis virus (IBV) in clinical samples from layer and broiler breeder farms in Chittagong district of Bangladesh. The samples were collected from 7 broiler and 3 layer breeder farms experiencing respiratory signs with elevated mortality. The virus was identified from trachea, lung and kidney...

The presence of endogenous viral elements in host genomes hints towards much older host-virus relationships than predicted by exogenous phylogenies, with highly mutable single-stranded DNA (ssDNA) viruses and RNA viruses often occupying entangled multispecies ecological niches. The difficulty lies in unravelling the long-term evolutionary history o...

Three complete genomes of beak and feather disease virus (BFDV) were recovered from wild twenty-eight parrots (Polytelis anthopeplus monarchoides). The genomes consisted of 1,996 bp with 1,934 identical sites and a typically content stem-loop structure between ORF1 and ORF2. This is the first report of BFDV infection as well as the complete genome...

The study was carried out to examine the pathological lesions in lungs of slaughtered cattle and buffalo carcasses in Chittagong Metropolitan area. A total of 882 (660 cattle and 222 buffalo) were examined in three different slaughterhouse where 128 (14.51%) cases were found with different pathological lesions in lungs. Among 128 (cattle 105 and bu...

Three complete genomes of beak and feather disease virus (BFDV) were recovered from wild twenty-eight parrots (Polytelis anthopeplus monarchoides). The genomes consisted of 1,996 bp with 1,934 identical sites and a typically content stem-loop structure between ORF1 and ORF2. This is the first report of BFDV infection as well as the complete genome...

The complete genome sequence of beak and feather disease virus (BFDV) from a wild Australian Mallee ringneck parrot (Barnardius zonarius barnardi) was characterized. The genome consists of 1,995 nucleotides and encodes two major proteins in opposing directions. This is the first evidence of BFDV infectivity and the first complete genome sequence fo...

A 6 year old female Spitz dog was admitted to Teaching Veterinary Hospital at Chittagong Veterinary and Animal Sciences University with the history of inappetance and asymmetric lower jaw. Oral inspection revealed a pair of pale enlarged lobulated mass around the third molar tooth of the left mandible. The dog was clinically diagnosed with epulis a...

A 6 year old female Spitz dog was admitted to Teaching Veterinary Hospital at Chittagong Veterinary and Animal Sciences University with the history of inappetance and asymmetric lowerjaw. Oral inspection revealed a pair of pale enlarged lobulated mass around the third molar tooth of the left mandible. The dog was clinically diagnosed with epulis an...

The object of this study was to report a post mortem findings of a female Indian Rock Python with a length of 406 cm (13.32 feet) and approximate weight of 60 kg (including a whole deer that was swallowed by the python), that was brought to the Teaching Veterinary Hospital, Chittagong Veterinary and Animal Sciences University (CVASU) by the Forest...

An epidemiological study was conducted on enteric helminth infections in stray dogs of four different thanas of Chittagong Metropolitan, Bangladesh. A total of 60 stray dogs were captured from the representative thanas in two consecutive seasons starting from May'2010 to February' 2011. The animals were captured, euthanized and necropsized for ente...

A hematological study was carried out to determine the considerable variations in blood parameters for brucellosis seropositivity in commercial dairy cattle in the Chittagong region of Bangladesh from January to May 2012. The study population comprised of 250 commercial cross-breed dairy cattle, randomly selected from 7 commercial farms. Milk Ring...

A one year (2009-10) prevalence study on gastrointestinal parasitism was conducted in crossbred and local cattle, Chittagong, Bangladesh. Fecal samples were collected randomly from 216 crossbred and 432 local cattle of four representative areas in three consecutive seasons. Samples were examined by routine coproscopical methods. The effects of topo...

A 9-year-old virgin German shepherd was admitted with continuous sanguinopurulent vaginal discharge, distended abdomen, and cachexia. The dog was clinically diagnosed with pyometra and successfully cured by ovario-hysterectomy. The results of histo-pathological examination showed that the endometrial glands were larger than normal and that part of...

An epidemiological survey was conducted to identify probable risk factors and prevalence of brucellosis in commercial and backyard dairy cows at Chittagong, Bangladesh. A total of 500 milk samples were collected (250 commercial and 250 backyards) for Milk Ring Test (MRT). The MRT positive cows were subjected to sera collection and Rose Bengal Plate...

A retrospective study was conducted to identify equine clinical diseases and their prevalence in farm conditions in Bangladesh from January'04 to December'10. Veterinary Hospital of RVFD, Equitation School, Dhaka and Riding School of BMA, Chittagong were the area of study. A total of 1026 cases of 41 different diseases and disorders were recorded....

A one year (2009-10) prevalence study on hemoprotozoan diseases was conducted in crossbred and indigenous cattle, Chittagong, Bangladesh. Blood samples were collected randomly from 216 crossbred and 432 indigenous cattle of four representative areas in three consecutive seasons. Samples were examined by Giemsa's stained blood smear method. The effe...

An epidemiological survey was conducted to identify probable risk factors and prevalence of brucellosis in commercial and backyard dairy cows at Chittagong, Bangladesh. A total of 500 milk samples were collected (250 commercial and 250 backyards) for Milk Ring Test (MRT). The MRT positive cows were subjected to sera collection and Rose Bengal Plate...

The research work was conducted to study the sequential pathological changes of Pullorum Disease (PD) and immunohistochemical detection of its causal agent (Salmonella pullorum) in various tissues of experimentally infected chicks. Total 40 day old (DO) chicks were divided into experimental and control groups. The chicks were infected at day 15 (D1...

A total of sixty adult stray dogs (32 male and 28 female) randomly captured from different locality of Chittagong metropolitan area were euthanized during the period of May 2010 to January 2011. The dogs were necropsized to observe the presence of Spirocerca lupi in their oesophagus producing characteristic nodular lesions. The associated haematolo...

The study was conducted to investigate the incidence of gastrointestinal parasites of buffalo in different slaughter houses of Chittagong Metropolitan Area, Bangladesh. A total of 100 faecal samples were collected from three slaughter houses and studied by coproscopic examinations. Direct smear, sedimentation and floatation techniques along with eg...

A zinc-selenium complex (Selcon<SUP>®</SUP>) was evaluated for the prevention of IBDV infection in broilers. Chicks were reared in relative isolation with optimum temperature, water and feeds. They were divided into 4 different groups named A1, B1, A2 and B2; having 3 replications each. Among them Groups A1 and B1 were uninfected group of which Gro...

The experiment was conducted at the dairy science and Microbiology Laboratory under the Department of Dairy and Poultry Science, Chittagong Veterinary and Animal Sciences University, Chittagong, Bangladesh during 10th August to 16th October 2008. The study was conducted to evaluate the quality of locally produced fluid milk consumed by the people o...