Shradha Subedi

Sunshine Coast Hospital and Health Service (Queensland Health) | SCHHS · Department of Medicine

Observational studies have found that placement of peripheral intravenous cannulas (PIVCs) in the antecubital fossa (ACF) is associated with increased risks of infection, including healthcare-associated Staphylococcus aureus bacteraemia (HA-SAB). Avoiding placement of the PIVC in the ACF area along with other preventive measures such as aseptic tec...

Observational studies have found that placement of peripheral intravenous cannulas (PIVCs) in the antecubital fossa (ACF) is associated with increased risks of infection, including healthcare-associated Staphylococcus aureus bacteraemia (HA-SAB). Avoiding placement of the PIVC in the ACF area along with other preventive measures such as aseptic tec...

Background Antimicrobial resistance (AMR) is an ever-increasing global health concern. One crucial facet in tackling the AMR epidemic is earlier and more accurate AMR diagnosis, particularly in the dangerous and highly multi-drug resistant ESKAPE pathogen, Pseudomonas aeruginosa . Objectives We aimed to develop two SYBR Green-based mismatch amplif...

Mycotic aneurysms are rare and if left untreated, can have devastating outcomes. In this case, a 72-year-old man presented to hospital with fevers, night sweats and abdominal pain. A CT scan revealed the development an infrarenal pseudoaneurysm over the course of 8 weeks, increasing from 2.8 cm to a 3.1 cm. The aneurysm was not present on a CT scan...

Third-generation cephalosporin-resistant (3GC-R) Enterobacteriaceae represent a major threat to human health. Here, we captured 288 3GC-R Enterobacteriaceae clinical isolates from 264 patients presenting at a regional Australian hospital over a 14-month period. Alongside routine mass spectrometry speciation and antibiotic sensitivity testing, isola...

Synopsis Third-generation cephalosporin-resistant (3GC-R) Enterobacteriaceae represent a major threat to human health. Here, we captured 288 3GC-R Enterobacteriaceae clinical isolates from 264 patients presenting at a regional Australian hospital over a 14-month period. Alongside routine mass spectrometry speciation and antibiotic sensitivity testi...

Infective endocarditis (IE) is associated with significant morbidity and mortality 1. We describe the local epidemiology, clinical characteristics, causative pathogens and clinical outcomes of patients treated for IE in the Sunshine Coast Hospital and Health Service (SCHHS) in Queensland, Australia. All patients with definite or probable IE by th...

Background: The prevalence of azole resistance in Aspergillus fumigatus is uncertain in Australia. Azole exposure may select for resistance. We investigated the frequency of azole resistance in a large number of clinical and environmental isolates. Methods: A. fumigatus isolates [148 human, 21 animal and 185 environmental strains from air (n = 6...

Shewanella algae is a rare pathogen related to water exposure in temperate climates. It is commonly associated with skin and soft tissue infections, peritonitis and bacteraemia. We report the first-ever case of S. algae infective endocarditis in a patient with previous splenectomy and explore the difficulties in treatment as well as highlight the i...

Background Aspergillus fumigatus causes infections ranging from invasive aspergillosis (IA) to chronic airway colonisation. Azoles are the backbone of therapy. Resistance of A. fumigatus to azoles varies substantively by region. Here we investigate the frequency of azole resistance in A. fumigatusin 2 Australian jurisdictions and characterise the r...

Blood-culture negative endocarditis (BCNE) accounts for up to 35% of all cases of infective endocarditis (IE) and is a serious life-threatening condition with considerable morbidity and mortality. Rapid detection and identification of the causative pathogen is essential for timely, directed therapy. Blood-culture negative endocarditis presents a di...

Accurate identification of slowly growing nontuberculous mycobacteria (SG-NTM) of clinical significance remains problematic. This study evaluated a novel method of SG-NTM identification by amplification of the mycobacterial 16S-23S rRNA internal transcribed spacer (ITS) region followed by resolution of amplified fragments by sequencer-based capilla...

Representative SCGE patterns of 17 species of clinical SG-NTM. A) M. paragordonae 361.65 bp B) M. gordonae SCGE type IV 362.03 bp C) M. kansasii 363.85 bp D) M. ulcerans 364.06 bp E) M. marinum 364.22 bp F) M. kubicae 365.57 bp G) M. avium SCGE type III 368.48 bp H) M. branderi 369.48 bp I) M. asiaticum 369.78 bp J) M. chimaera 370.60 bp K) M. intr...

Infections due to Scedosporium/Pseudallescheria species are increasingly recognized in both immunocompromised and immunocompetent hosts. Patients with organ and stem cell transplants and malignancy are at high risk for infection. Infection is also acquired through trauma and near-drowning incidents. Scedosporium apiospermum complex and Lomentospora...

Outpatient parenteral antibiotic therapy (OPAT) has become established as a standard of care in most Australian hospitals to treat a variety of infections. Since 1998, the Alternate Site Infusion Service (ASIS) has provided an OPAT service to five hospitals in southern Brisbane, Queensland, using predominantly a patient or carer administration mode...

We report three cases of lymphocutaneous infection caused by the thermally dimorphic fungus, Sporothrix schenckii from Australia's tropical Northern Territory. Two cases were acquired locally, making them the first to be reported from this region. All three cases presented with ulceration in the limb; however, the classical sporotrichoid spread was...

Coccidioidomycosis is a fungal infection caused by Coccidioides species. The disease has wide clinical presentation and a distinct geographical distribution. We describe two cases of coccidioidomycosis in returned Australian travellers who presented to Nambour Hospital. Knowledge of the international geographical distribution of endemic fungal infe...