Shoko Ueki

Shoko Ueki
Okayama University · Institute of Plant Sciences and Resources

PhD

About

51
Publications
4,329
Reads
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1,237
Citations
Introduction
Shoko Ueki currently works at the Institute of Plant Sciences and Resources, Okayama University. Her current project is 'Ecophysiology of Heterosigma akashiwo'.
Additional affiliations
September 2017 - present
Okayama University
Position
  • Professor (Associate)
Description
  • Ecophysiology of Heterosigma akashiwo, algal bloom dynamics
September 2011 - present
Okayama University
Position
  • Professor (Assistant)
September 1998 - August 2011
Stony Brook University
Position
  • research assistant professor

Publications

Publications (51)
Article
Full-text available
Phytoplankton blooms, including harmful algal blooms (HABs), have serious impacts on ecosystems, public health, and productivity activities. Rapid detection and monitoring of marine microalgae are important in predicting and managing HABs. We developed a toolkit, the Suitcase Lab, to detect harmful algae species in the field. We demonstrated the Su...
Article
Full-text available
Harmful algae blooms (HABs) cause acute effects on marine ecosystems due to their production of endogenous toxins or their enormous biomass, leading to significant impacts on local economies and public health. Although HAB monitoring has been intensively performed at spatiotemporal scales in coastal areas of the world over the last decades, procedu...
Article
The global distribution of phytoplankton is defined by many events, including long‐term evolutionary processes and shorter time span processes (e.g., global climate change). Furthermore, human assisted, unintentional dispersion, including the transport of live fish and spat for aquaculture, and transfer of aquatic microorganisms contained in ship b...
Article
Full-text available
Information related to geographical distribution and local strain composition is essential to an understanding of the dynamics of harmful algae in the environment. Previously, we identified a highly variable segment on the mitochondrial genome of Heterosigma akashiwo, a bloom-forming noxious unicellular algal species. Here, we assessed the utility...
Article
Full-text available
We report here the complete chloroplast genome sequences of seven strains of the bloom-forming raphidophyte Heterosigma akashiwo . These ~160-kb sequences contain 124 protein-, 6 rRNA-, and 34 tRNA-coding sequences. Notable sequence variations were observed among these seven sequenced and two previously characterized strains.
Article
Full-text available
Research Cite this article: Higashi A, Nagai S, Seone S, Ueki S. 2017 A hypervariable mitochondrial protein coding sequence associated with geographical origin in a cosmopolitan bloom-forming alga, Heterosigma akashiwo. Biol. Lett. 13: 20160976. http://dx. Geographical distributions of phytoplankton species can be defined by events on both evolutio...
Article
Full-text available
We report here the complete mitochondrial genome sequences of four strains of bloom-forming raphidophytes from Heterosigma akashiwo . These 39-kb sequences contain 42 protein-, two rRNA-, and 26 tRNA-coding sequences. Notable sequence variations were observed among these four newly sequenced and three previously characterized strains, suggesting th...
Article
Algal bloom is typically caused by aberrant propagation of a single species, resulting in its predomination in the local population. While environmental factors including temperature and eutrophication are linked to bloom, the precise mechanism of its formation process is still obscure. Here, we isolated a bacterial strain that promotes growth of H...
Article
Full-text available
Nucleocytoplasmic DNA viruses are a large group of viruses that harbor double-stranded DNA genomes with sizes of several 100 kbp, challenging the traditional concept of viruses as small, simple ‘organisms at the edge of life.’ The most intriguing questions about them may be their origin and evolution, which have yielded the variety we see today. Sp...
Article
Full-text available
We report the complete genome sequence of Heterosigma akashiwo virus strain 53. The virus is a member of the Phycodnaviridae, one of the families regarded as giant double-stranded DNA viruses. The 274,793-bp genome contained 246 protein-coding and 3 tRNA-coding sequences.
Article
Full-text available
Encased in rigid cell walls, plant cells have evolved unique channel structures, plasmodesma (Pd), to create a pathway for molecular exchange between adjacent cells. Pd are basically cytoplasmic channels through the cell wall, which are lined by plasma membrane, and contain a modified strand of ER that spans them. These structures provide cytoplasm...
Article
Full-text available
Mirafiori lettuce big-vein virus (MiLBVV) is a member of the genus Ophiovirus, which is a segmented negative-stranded RNA virus. In microprojectile bombardment experiments to identify a movement protein (MP) gene of ophioviruses that can trans-complement intercellular movement of an MP-deficient heterologous virus, a plasmid containing an infectiou...
Article
Transient gene expression is a useful approach for studying the functions of gene products. In the case of plants, Agrobacterium infiltration is a method of choice for transient introduction of genes for many species. However, this technique does not work efficiently in some species, such as Arabidopsis thaliana. Moreover, the infection of Agrobact...
Article
Full-text available
The turnover of callose (β-1,3-glucan) within cell walls is an essential process affecting many developmental, physiological and stress related processes in plants. The deposition and degradation of callose at the neck region of plasmodesmata (Pd) is one of the cellular control mechanisms regulating Pd permeability during both abiotic and biotic st...
Article
Full-text available
Validating interactions between different proteins is vital for investigation of their biological functions on the molecular level. There are several methods, both in vitro and in vivo, to evaluate protein binding, and at least two methods that complement the shortcomings of each other should be conducted to obtain reliable insights. For an in vivo...
Article
Full-text available
Cell-to-cell signal transduction is vital for orchestrating the whole-body physiology of multi-cellular organisms, and many endogenous macromolecules, proteins, and nucleic acids function as such transported signals. In plants, many of these molecules are transported through plasmodesmata (Pd), the cell wall-spanning channel structures that interco...
Data
ANK cDNA sequence. The nucleotide sequence encoding the ankyrin repeat-containing domain (positions 670–1032) is shadowed, and the sequence used for the pRNAi-ANK construct is underlined. The segment used for pRNAi-ANK is unique to ANK, except for the 17-bp region highlighted in black (positions 19–45), which is found in two N. tabacum genes, aspar...
Data
RNAi transgenic plants with severe and moderate suppression of ANK expression. (A) Quantification of the levels of ANK transcripts with indicated standard deviations. Plants with ANK. gene expression levels reduced to 5% and 15–40% of the wild-type expression level were designated severe and moderate suppressors, respectively. Plants were analyzed...
Data
Altered ANK expression levels do not affect MP-YFP targeting to PD or protein expression. (A–F) PD localization of MP-YFP at 16 h (A–C) and 24 h (D–F) after bombardment in wild type plants (A, D), RNAi ANK2 (B, E), and ANK1 (C, F). Bars = 20 µm. Plastid autofluorescence is in white. All images are single confocal sections. (G) MP-YFP expression lev...
Data
Supplemental Methods (0.02 MB DOC)
Data
Histochemical detection of ROS. The leaf discs were excised from the indicated plant lines and treated with DAB. No significant difference in ROS levels were observed between plant lines with different expression levels of ANK. WT; wild type. RNAi1 and 2; RNAi ANK1 and RNAi ANK2, respectively. (2.62 MB TIF)
Data
Relative expression levels of N. tabacum ASPARTATE AMINOTRANSFERASE (AATF) and MAGNESIUM PROTOPORPHYRIN IX (MgPP) genes in wild-type, RNAi ANK1 and RNAi ANK2 plants. AThe shown values were normalized to the amounts of ACTIN transcript in the same samples. BWT, wild-type. CStandard deviations are indicated. DP-values for the sets of data obtained fr...
Data
Positive correlation between ANK-StrepII transcripts and protein levels. (A) Western blot analysis of ANK-StrepII in extracts prepared from the independent transgenic lines ANK-StrepII 1 to 6. (B) qRT-PCR analysis of the ANK-StrepII mRNA levels in the same transgenic lines. The shown values were normalized to the amounts of ACTIN transcript in the...
Article
Full-text available
Plasmodesma (PD) is a channel structure that spans the cell wall and provides symplastic connection between adjacent cells. Various macromolecules are known to be transported through PD in a highly regulated manner, and plant viruses utilize their movement proteins (MPs) to gate the PD to spread cell-to-cell. The mechanism by which MP modifies PD t...
Article
Full-text available
Here, we present a simple and rapid protocol to detect and assess the extent of cell-to-cell macromolecular transport in planta. In this protocol, a fluorescently tagged-protein of interest is transiently expressed in plant tissue following biolistic delivery of its encoding DNA construct. The intra- and intercellular distribution of the tagged pro...
Article
Full-text available
Transient gene expression is an indispensable tool for studying functions of gene products. In the case of plants, transient introduction of genes by Agrobacterium infiltration is a method of choice for many species. However, this technique does not work efficiently in Arabidopsis leaf tissue, the most widely used model system for basic plant biolo...
Chapter
Viral long distant transport is an essential step for systemic infection. Because the process involves different types of highly differentiated vascular-associated cells, the virus systemic movement is regulated differentially at each tissue interface. In this chapter, we review current knowledge about viral systemic transport process in non-Arabid...
Chapter
After initial inoculation, most viruses spread in host plants via two mechanisms: local, cell-to-cell movement and systemic movement. Cell-to-cell movement occurs through intercellular connections, plasmodesmata (PD), between epidermal (EP) cells and mesophyll (MS) cells, or MS cells and MS cells. Systemic movement is more complex, comprising three...
Article
Full-text available
Cadmium-induced glycine-rich protein (cdiGRP) is a cell wall-associated factor that increases callose levels in plant vasculature. To better understand the cdiGRP/callose regulation system, we identified a tobacco protein, GrIP (cdiGRP-interacting protein, GrIP), that associates with cdiGRP and localizes at the plant cell wall. Constitutive overexp...
Article
Propagation of viral infection in host plants comprises two distinct and sequential stages: viral transport from the initially infected cell into adjacent neighboring cells, a process termed local or cell-to-cell movement, and a chain of events collectively referred to as systemic movement that consists of entry into the vascular tissue, systemic d...
Article
Full-text available
Systemic movement is central to plant viral infection. Exposure of tobacco plants to low levels of cadmium ions blocks the systemic spread of turnip vein-clearing tobamovirus (TVCV). We identified a tobacco glycine-rich protein, cdiGRP, specifically induced by low concentrations of cadmium and expressed in the cell walls of plant vascular tissues....
Article
Although long-distance movement of endogenous mRNAs in plants is well established, the functional contributions of these transported RNA molecules has remained unclear. In a recent report, Kim et al.2001 showed that systemically transported mRNA is capable of causing phenotypic change in developing tissue. Here, this finding and its significance ar...
Article
Full-text available
Post-transcriptional gene silencing (PTGS) is an important mechanism for regulation of plant gene expression and virus-plant interactions. To better understand this process, the heavy metal cadmium was identified as a specific inhibitor in two different PTGS systems, constitutive and inducible. The pattern of cadmium-induced inhibition of PTGS allo...
Article
Transglutaminases (TGases) are enzymes which catalyze cross-link formation between glutamine residues and lysine residues in substrate proteins. We have previously reported that one of the TGases, blood coagulation factor XIIIa (FXIIIa), is capable of mediating adhesion of various cells. In this paper, we report for the first time that tissue-type...
Article
We report here that the 12-lipoxygenase metabolite of arachidonic acid, 12-hydroxy-5Z, 8Z, 10E, 14Z, eicosatetraenoic acid (12-HETE), stimulates cAMP production in human fibroblasts among various cultured cell lines tested. Although 12-HETE seemed to stimulate the phospholipase C (PLC)-protein kinase C (PKC) system, inhibitors against PLC and PKC d...
Article
Full-text available
Integrin heterodimers undergo a conformational change upon the binding of ligand to their extracellular domains. An anti-beta1 integrin monoclonal antibody AG89 can detect such a conformational change since it recognizes a ligand-inducible epitope in the stalk-like region of beta1 subunits. The binding of a 125I-labeled AG89 Fab fragment to alpha5...
Article
We compared the effects of two anti-β1 integrin activating antibodies, TS2/16 and AG89, on K562 cell adhesion to fibronectin. Though both antibodies effectively induced cell adhesion, the EC50 for AG89 was more than 200-fold higher than that for TS2/16. Scatchard analysis of the data from [125I]Fab fragment binding to the cells revealed that the TS...
Article
Full-text available
Transglutaminases (TGases) are enzymes which catalyze cross-link formation between glutamine residues and lysine residues in substrate proteins. In the present study, we report for the first time that a representative enzyme, blood coagulation factor XIIIa (FXIIIa), is capable of mediating adhesion of various cells. When coated on plastic surfaces...
Article
von Willebrand factor is a large multimeric plasma protein which plays important roles in platelet aggregation, blood coagulation and probably also in the adhesion of endothelial cells. A 100-kDa propeptide, called the propolypeptide of von Willebrand factor (pp-vWF), is generated during biosynthesis. We found that pp-vWF served as a substrate for...
Article
von Willebrand factor is a large multimeric plasma protein which plays important roles in platelet aggregation, blood coagulation and probably also in the adhesion of endothelial cells. A 100-kDa propeptide, called the propolypeptide of von Willebrand factor (pp-vWF), is generated during biosynthesis. We found that pp-vWF served as a substrate for...

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Projects

Projects (4)
Project
Development of harmful algal bloom (HAB) monitoring methods and forecast system for sustainable aquaculture and coastal fisheries in Chile (Monitoring of algae in Chile)