Shifu Aggarwal

Shifu Aggarwal
Houston Methodist Hospital | HOUSTONMETHODIST

PhD Biotechnology

About

9
Publications
2,752
Reads
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60
Citations
Citations since 2017
9 Research Items
60 Citations
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2017201820192020202120222023051015202530
Introduction

Publications

Publications (9)
Article
Full-text available
The coronavirus disease 2019 (COVID-19) pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is a major global health concern. This virus infects the upper respiratory tract and causes pneumonia-like symptoms. So far, few studies have shown alterations in nasopharyngeal (NP) microbial diversity, enrichment of opportunisti...
Article
Full-text available
During the course of the COVID-19 pandemic, large-scale genome sequencing of SARS-CoV-2 has been useful in tracking its spread and in identifying variants of concern (VOC). Viral and host factors could contribute to variability within a host that can be captured in next-generation sequencing reads as intra-host single nucleotide variations (iSNVs)....
Preprint
Full-text available
Background The coronavirus disease 2019 (COVID-19) pandemic caused by severe acute respiratory syndrome corona virus 2 (SARS-CoV-2) is a major global health concern. This virus infects the upper respiratory tract and causes pneumonia-like symptoms. So far, few studies have shown alterations in nasopharyngeal (NP) microbial diversity, enrichment of...
Preprint
Full-text available
The coronavirus disease 2019 (COVID-19) pandemic caused by severe acute respiratory syndrome corona virus 2 (SARS-CoV-2) is a major global health concern. This virus infects the upper respiratory tract and causes pneumonia-like symptoms. So far, few studies have shown that respiratory infections alter nasopharyngeal (NP) microbiome diversity and en...
Preprint
Full-text available
Vaccine breakthrough infections pose a vast challenge in the eradication of the COVID pandemic situation. Emerging SARS-CoV-2 variants of concern infecting the immunized individuals indicate an ongoing battle between host immunity and natural selection of the pathogen. Our report sheds light on the prominent SARS-CoV-2 variations observed in the is...
Article
Full-text available
Extracellular vesicles (EV), also known as membrane vesicles, are produced as an end product of secretion by both pathogenic and non-pathogenic bacteria. Several reports suggest that archaea, gram-negative bacteria , and eukaryotic cells secrete membrane vesicles as a means for cell-free intercellular communication. EVs influence intercellular comm...
Article
Full-text available
Staphylococcus aureus is the primary cause of nosocomial infections. It produces potent toxins and causes superficial lesions, systemic diseases, and several toxaemic syndromes. In this study, we determined the role of efflux pump in conferring resistance to fluoroquinolones in the biofilm of methicillin resistant Staphylococcus aureus (MRSA) and m...
Article
Full-text available
Staphylococcus aureus is one of the major causes of nosocomial infections. This organism produces powerful toxins and cause superficial lesions, systemic infections, and several toxemic syndromes. A total of 109 S. aureus strains isolated from a variety of infections like ocular diseases, wound infection, and sputum were included in the study. Mini...

Questions

Questions (10)
Question
At different time points I want to isolate RNA from biofilm with and without inhibitor from Staphylococcus aureus
Question
A paper suggested that efflux pumps abolishes biofilm formation in gram-negative bacteria, is it possible that in Staphylococcus aureus same mechanism can occur?
Question
I have tried to isolate eDNA from S aureus biofilm, but all methods failed.
Kindly suggest me some protocol.
Question
I am trying to isolate the proteins from Staphylococcus aureus for Mass Spectrometry by acetone precipitation method. Though I get a pellet but when I check its OD by Bradford assay, it is showing no concentration.
Can somebody suggest a protocol for the same.
Question
I have been trying to electroporation Staphylococcus aureus isolate in pFA545 which has tetracycline resistance marker. I am taking 2.5ug/ml concentration of tetracycline in tryptic soy agar. Though my isolate is highly susceptinle to tetracycline but when I am plating on tetracycline (2.5ug/ml) plate I am getting a lawn. So, how much should I increase the concentration of tetracycline. Is increasing the concentration will have any effect on my isolate.
Question
I have extracted the EPS from Staphylococcus aureus isolates and I have found the amount of DNA, protein and polysaccharides. By any means can I find a link between EPS and efflux pumps..?
Question
I am trying to find a relation between efflux genes and eDNA in biofilm matrix of Staphylococcus aureus. 
Question
I want to clone a gene in s aureus RN4220, and check its expression. previously I tried with pET28c, but it didnt work. So which expression vector should I choose
Question
I have isolated a plasmid from s aureus clinical isolate, I want to characterize it so as to determine whether it is a expression vector. 
Question
I have ligated my gene with an expression vector pet28c, and I am trying to electroporate it in S aureus Mu50, but when I did this, I am getting a lawn on the plates. Can someone suggest me with a solution for this.

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Projects

Project (1)
Project
The mechanism of efflux pump in biofilm formation and how it is leading to antibiotic resistance