Sherifah Monilola Wakil

Sherifah Monilola Wakil
University of Ibadan · Department of Microbiology

PhD, Industrial Microbiology

About

58
Publications
36,455
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391
Citations
Citations since 2016
27 Research Items
333 Citations
20162017201820192020202120220102030405060
20162017201820192020202120220102030405060
20162017201820192020202120220102030405060
20162017201820192020202120220102030405060
Education
March 1998 - May 2006
University of Ibadan
Field of study
  • Microbiology

Publications

Publications (58)
Article
The link between increasing global population, food demand, industrialization, and agricultural waste is strong. Decomposing by-products from food cycles can introduce harmful toxic heavy metals, active degrading microbes, and enzymes to the environment. Additionally, high greenhouse gas emissions from the decomposing wastes contribute to global ch...
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The aim of this study was to determine the potential of keratinases produced by Bacillus species isolated from poultry feather waste in stain removal. The effect of individual and combined detergent components on the keratinase activity of EZYKer-51, EZYKer-50 and EZYKer-53 enzymes produced from keratin degrading Bacillus licheniformis-K51, Bacillu...
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Maize straw is a lignocellulosic waste that is annually added to the environment as pollutant because its accumulation constitutes a nuisance and in addition, its reducing sugar is not readily released. Pretreatment of maize straw makes its reducing sugar available for fermentation into bio-products such as bioethanol production under optimized con...
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Bacterial keratinases are important hydrolytic enzymatic tools produced only in the presence of keratin-containing substrates such as feathers. Accumulation of chicken feather waste generated by commercial poultry processing plants leads to wastage of the protein-rich substrate. Keratinase producing bacteria can serve an important role in the remov...
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Maize straw (MS) is a lignocellulosic substrate that constitutes huge wastes in the environment. This work aimed to pretreat MS with mushroom alone as a biological agent, and with NaOH prior to mushroom treatment (combined chemical and biological), and subsequently converting the released reducing sugars (RS) to ethanol using Saccharomyces cerevisi...
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Keratins are tough, fibrous proteins found as the major protein component in hair. Chemical hair removal is harsh and results in severe toxicity and pollution, hence, a need for a non-chemical enzymatic hair removal process. The aim of this study is to characterise keratinase from isolated keratin-degrading Bacillus licheniformis-K51, Bacillus subt...
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Aims: This study aimed at screening, producing and characterizing polyhydroxyalkanoates (PHA) from lactic acid bacteria (LAB) isolated from dairy wastewater, fermented cow milk and "ogi". Place and Duration Methodology: The initial screening of isolated LAB was carried out by Sudan Black staining method followed by secondary screening on liquid med...
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Aim: This work was carried out to investigate the influence of Lactic Acid Bacteria (LAB) on organoleptic quality and proximate composition of yoghurt, and viability of starter cultures in yoghurt. Methods: The LAB starter cultures were selected based on their ability to produce diacetyl and lactic acid. Results: Lactobacillus caseiN1 produced the...
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Tannases are enzymes that catalyze the production of gallic acid which is a versatile precursor of various chemicals used in food and pharmaceutical industries. This work is aimed at isolation and production of tannase from soil fungi. Moulds were isolated from soil samples that were collected from different sites in Ibadan Metropolis. Isolated fun...
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Aim: This study focuses on the screening and characterisation of keratin-degrading Bacillus species from feather waste. Methods: Nine bacteria were isolated from feather waste obtained from a poultry layout at Egbeda local government secretariat, Ibadan, Nigeria. These bacteria were grown in basal medium with feather as primary source of carbon, ni...
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Abstract: One of the most significant ways of improving the quality of foods is through fermentation. This study aimed at isolating LAB with probiotic properties with potential as starter culture in foods. LAB isolates were cultured on MRS agar from fermenting Acha (Digitaria exilis), with pure culture obtained via subsequent streaking. Pure isolat...
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This study was carried out to investigate the antagonistic effect of probiotic yoghurt against some selected food-borne pathogens during cold storage. Probiotic yoghurt was produced from processed cow milk using controlled fermentation, and later inoculated with selected food-borne pathogens at inoculum level of 10 5 CFU/mL. The antagonistic effect...
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Lactic acid bacteria are known to have probiotic attributes which are beneficial to human. This study was embarked upon to screen lactic acid bacteria (LAB) from raw milk and fermented milk (nono) samples for probiotic potential. Some of the assessed probiotic qualities include antimicrobial activities against food-borne pathogens, survival at acid...
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Aim: This study focuses on the screening and characterisation of keratin-degrading Bacillus species from feather waste. Methods: Nine bacteria were isolated from feather waste obtained from a poultry layout at Egbeda local government secretariat, Ibadan, Nigeria. These bacteria were grown in basal medium with feather as primary source of carbon, ni...
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The effective fermentation of cellulose remains an intractable challenge in food industry. The relevant cellulase enzyme is also in a high demand from industry for several biotechnological applications that inevitably in recent times led to many efforts for screening some yeasts for better cellulase production and fermentation performance. Plant−fe...
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Lipases (triacylglycerol acylhydrolases, EC3.1.1.3) are water-soluble enzymes that catalyze the hydrolysis (and synthesis) of ester bonds formed from acyl glycerol and long-chain fatty acids at lipid-water interface. Palm oil mill effluents have been reported to be rich in lipolytic bacteria, thus this work aimed at production, characterization and...
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Aim: The aim of the study is to produce and characterize dextranase produced by fungi isolated from soil and honey. Place and Duration of Study: The study was carried out at the Department of Microbiology, University of Ibadan, between April, 2017 and October, 2017. Methodology: Garden soils were collected from different locations within the Univer...
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Abstract The effects of starter fermentation on the nutritional qualities of maize –tigernut fortified weaning foods were investigated. The dry-milled, malted maize grains fortified with dry-milled roasted tigernut flours (70:30) was subjected to 48hrs of spontaneous and starter fermentation (singly and as combined starters). Nutritional, sensory c...
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Laccases are multi copper oxidases having wide substrate specificity for phenolic compounds, amines, inorganic compounds like iodine, using oxygen and producing water as by product. The aim of this study is to produce, characterize and purify laccase from yeast isolated from different samples. Yeast isolates were screened for laccase production usi...
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Laccases (EC.1.10.3.2 are multi-phenolics and non-phenolic oxidizing enzymes) have received much attention in their use for environmental bioremediation, food quality and functionality improvement. This work was aimed at degrading polycyclic aromatic hydrocarbons and aroma oxidation of fermented locust beans seeds ‘iru’ by laccase produced by yeast...
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Abstact Catalytic activity and purification of thermostable lignases from Ganoderma sp. ADEB468 and the potential of the enzyme in dye decolourization were investigated. The highest catalytic activity of laccase (Lac) (175.56 U/L), lignin peroxidase (LiP) (1313.98 U/L), and manganese peroxidase (MnP) (92.27 U/L) was supported by 0.1mM Na + , 5.0 mM...
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Fe(II), Co(II), Ni(II), Cu(II) and Zn(II) complexes of the mixed ligands-N, N'-dimethyl dithiocarbamic and benzoic acids were synthesized and characterised by electronic and IR spectroscopies, percentage metal analysis, conductance, magnetic moments and melting point measurements. Percentage metal analysis, conductance and magnetic moment data indi...
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Some metal(II) mixed ligand complexes of nicotinic and dimethyldithiocarbamic acids were synthesized (M = Mn, Fe, Co, Ni, Cu and Zn) and characterised by solubility, percentage metal analysis, conductivity, infrared and electronic spectra measurement. Electronic spectra and room temperature magnetic moments indicated that the metal complexes mostly...
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Mn(II), Fe(II), Ni(II), Cu(II) and Zn(II) mixed ligand complexes of Paracetamol (HL) and Benzoic acid (HL 1) were synthesized and characterized by room temperature magnetic moments, melting points, conductance measurements, infrared and electronic spectroscopies. The molar conductance measurements in DMSO indicated that metal(II) complexes were cov...
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Mixed drug metal(II) complexes of Trimethoprim (TMP, HL) and Sulfamethoxazole (SMX, HL) were 1 synthesized and characterized by percentage metal, infrared and electronic spectroscopies, room temperature magnetic moments, melting points and conductance measurements. The metal analysis revealed that the complexes analyze as [M(HL)(HL)X].nH O,where M...
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Mn(II), Fe(II), Co(II), Ni(II), Cu(II) and Zn(II) complexes of the mixed drugs, Riboflavin (Vitamin B2) and Nicotinic acid (Vitamin B3) were synthesized and characterized by percentage metal analysis, infrared and electronic spectroscopies, melting point, solubility, molar conductance and room temperature magnetic moment measurements. The percentag...
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L-Asparaginase producing bacteria were isolated from soil samples under optimum conditions using submerged fermentation. Their abilities to produce asparaginase at different pH, temperature, incubation temperature, utilization of different carbon and nitrogen sources as well as their specific conditions for optimal activity were also investigated....
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Studies on synthesis, characterization, antimicrobial and antioxidant properties of some metal(II) complexes of the mixed ligands, Vitamins B2 (Riboflavin, HL) and Benzoic acid (HL2), where M = Mn, Fe, Co, Ni, Cu and Zn were carried out. The conductivity measurements and percentage metal analysis confirmed that the metal complexes were covalent in...
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Some metal(II) complexes of mixed ligands-dimethydithiocarbamic and paminobenzoic acids were synthesized (M = Mn, Fe, Co, Ni, Cu and Zn) and characterized by solubility, percentage metal, conductivity, infrared and electronic spectra measurements. Infrared data showed that the dimethydithiocarbamic and paminobenzoic acids coordinated through the su...
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Mn(II), Fe(II), Co(II), Ni(II), Cu(II) and Zn(II) complexes of mixed ligands, Riboflavin (HL) and 4-aminobenzoic acid (HL1) were synthesized and characterized by percentage metal, infrared and electronic (solid reflectance) spectroscopies, room temperature magnetic moments, melting points and conductance measurements. The conductance measurements i...
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"Pupuru" is a traditional fermented, smoked-dried cassava food, commonly consumed in South western, Nigeria and other West Africa countries. Fresh cassava variety, TMS 30572 was peeled, washed, submerged in sterile distilled water and inoculated aseptically with 1ml of inoculums starter microorganisms. Fermentation was allowed for 5 days at room te...
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Lactic acid bacteria (LAB) responsible for spontaneous fermentation of cucumber were isolated and their antimicrobial producing potentials were screened against 10 indicator strains. 65% of the isolated LAB produced antimicrobial activities against at least two indicator strains. The indicator strains used were: Escherichia coli, Bacillus lichenifo...
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Mn(II), Fe(II), Co(II), Ni(II), Cu(II) and Zn(II) complexes of mixed drug Paracetamol (HL) and Sulfamethoxazole (HL 1) were synthesized and characterized by room temperature magnetic moments, melting points, percentage metal, conductance measurements, infrared and electronic spectroscopies. The percentage metal analysis showed that the complexes mo...
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Fermented tigernut milk (FTM) was prepared from three different varieties (fresh yellow, big and small dry brown) of tigernut (Cyperus esculenta) obtained from Bodija market, Ibadan, Oyo state. Fifty two microbial isolates were obtained from FTM at different fermentation times, 32 of which were on MRS agar, 12 on nutrient agar and 8 on malt extract...
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Objective: To determine the best starchy food substrate for the fermentative production of lactic acid. Methodology and Results: Five starchy-based food substrates (maize ogi, sorghum ogi, millet ogi, mashed cassava for gari production and steeped cassava for fufu production) were prepared fresh and allowed to ferment spontaneously for a period of...
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The mineral and vitamin contents of the fermented formulated infant weaning food developed from millet, cowpea, groundnut and Moringa oleifera leaves are reported in this study. For most of the developed blends, the mineral contents increased within the first 48 hrs of fermentation but decreased by 72 hrs of fermentation time. Generally, supplement...
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The feasibility of bioethanol production from spontaneous fermentation of palm oil mill effluent was carried out. Cooled POME (1.5L) was allowed to ferment spontaneously for 21 days for analysis of microbial quality, free fatty acid, lipase activity and ethanol contents. Effect of pH and supplementation on ethanol and other parameters were also det...
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The mycological and physicochemical qualities (pH and titratable acidity) of fermenting pearl millet gruel were evaluated using routine methods. Several species of yeasts and moulds were isolated and the mould species identified based on their observable macroscopic and microscopic characteristics. The moulds identified included: Penicillium sp (FM...
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Plant tissue culture technology has been successfully used for the commercial production of pathogen-free plants and for germplasm conservation of rare and endangered species. Clean cultures of aseptically micropropagated shoot cultures of the genus Dioscorea (yam) grown on yam multiplication media are often contaminated with covert bacteria. The b...
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Twelve maize samples were collected from four different Local Government Areas within Ibadan Metropolis Oyo state, Nigeria and their moisture content was determined. The samples were also screened for aflatoxigenic fungi and aflatoxin contamination and also the presence of other fungi. The results of the moisture content revealed that the moisture...
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In vitro germplasm collections are always under the threat of air-borne microbial contaminants following poor laboratory practices and endogenous contaminants.Visibly clean cultures of aseptically micropropagated shoot cultures of plants of the genus Dioscorea (yam) grown on yam multiplication media are often contaminated with covert bacteria. The...
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Infant weaning food was produced from sorghum -cowpea blends fermented with four combinations of starter organisms (Lactobacillus plantarum and Saccharomyces cerevisiae (AB1), Lactobacillus plantarum and Pediococcus acidilactici (AB2), Pediococcus acidilactici and Saccharomyces cerevisiae (AB3), and Lactobacillus plantarum, Pediococcus acidilactici...
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Full-text available
Twelve maize samples were collected from four different Local Government Areas within Ibadan Metropolis Oyo state, Nigeria and their moisture content was determined. The samples were also screened for aflatoxigenic fungi and aflatoxin contamination and also the presence of other fungi. The results of the moisture content revealed that the moisture...
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Full-text available
A total of ten isolates were obtained from rotted feathers and soils from a local poultry farm waste at Bodija in Ibadan and screened for proteolytic activity and feather degradation. Selected isolates were identified as Bacillus licheniformis (E1), Bacillus subtilis A (D1), Bacillus subtilis B (D1W), Bacillus cereus (B1), Pseudomonas aeruginosa (E...
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Pectinolytic organisms are a constant threat with regard to spoilage of vegetables because of their extensive host range and widespread distribution. It is estimated that between 10 and 30% of fresh vegetables are wasted, mainly due to three factors: mechanical injuries, physiological decays and microbial spoilage. The role of microorganisms in thi...
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The total lactic acid bacterial community involved in the spontaneous fermentation of malted cowpea fortified cereal weaning food was investigated by phenotypically and cultivation independent method. A total of 74 out of the isolated 178 strains were Lactobacillus plantarum, 32 were Pediococcus acidilactici and over 60% were Lactobacillus strains....
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Post harvest deterioration by microbes due to improper storage condition is considered to be the major cause of spoilage of most seeds like cashew nuts. Roasted cashew nuts were subjected to 4 different storage conditions with different relative humidity of 30, 70, 80 and 90%, respectively, for a period of 12 days. Each storage condition was examin...
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Post harvest deterioration by microbes due to improper storage condition is considered to be the major cause of spoilage and rancidity of most oil-bearing seeds like cashew nuts through lipolytic action of lipase enzyme. Roasted cashew nuts were subjected to four different storage conditions with different relative humidity of 30, 70, 80 and 90%, r...
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Cereal samples, specifically maize DMR-ESR-Y, KSV-15 (sorghum) and millet as well as legume (cowpea variety Ife Brown) were soaked and malted at room temperature (28 ± 2 o C). Dried malted samples were separately milled, formulated by a mix with ratios 70/30 (cereals/ cow-peas) and subjected to natural fermentation for 72 h. There was a drastic inc...
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Phenotypic identification and monitoring of the dynamics of naturally occurring microbial community responsible for the spontaneous fermentation of different cereal-legume weaning blends was carried out. Enumeration using culture-dependent method showed that cell counts increased within the first 24 h with the highest total viable count of 1.2 × 10...
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Different cereal-legume weaning blends were formulated and subjected to spontaneous fermentation for a period of 72 h. The analysis of the DGGE pattern, of the fermented blends, obtained with bacterial primer targeting the 16S rDNA genes clearly demonstrated that there was a major shift in the community structure within the first 24 h. The species...
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"Kunu-tsamia" is a traditional non-fermented carbo-hydrate-rich meal produced from millet Pennisetum glau-cum, which is consumed mainly in Northern Nigeria. We embarked upon fermentation process to study the succes-sional pattern of microorganisms, shelf-life and sensory attributes. Therefore, microbiological status, sensory properties and shelf-li...

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