Sheena M Cruickshank

Sheena M Cruickshank
The University of Manchester · Faculty of Biology Medicine and Health

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122
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Publications

Publications (122)
Article
Full-text available
Cities must address many challenges including air quality, climate change and the health and wellbeing of communities. Public authorities and developers increasingly look to improve these through the implementation of interventions and innovations, such as low traffic neighbourhoods, deep housing retrofits and green infrastructure. Monitoring the i...
Article
Full-text available
Schistosomiasis control requires multisectoral approaches including praziquantel treatment, access to safe water, sanitation and hygiene, and health education. Community input can help ensure health education programs are culturally appropriate to effectively direct protective behavior change. This study reports on the three-stage development of an...
Article
Non-healing wounds are a major area of unmet clinical need remaining problematic to treat. Improved understanding of pro-healing mechanisms is invaluable. The enzyme arginase1 is involved in pro-healing responses with its role in macrophages best characterized. Arginase1 is also expressed by keratinocytes; however, arginase1 function in these criti...
Article
Full-text available
Arginase (ARG) represents an important evolutionarily conserved enzyme that is expressed by multiple cell types in the skin. Arg acts as the mediator of the last step of the urea cycle, thus providing protection against excessive ammonia under homeostatic conditions through the production of L-ornithine and urea. L-ornithine represents the intersec...
Article
Parasitic infections can be challenging to study because two dimensional light and electron microscopy are often limited in visualising complex and inaccessible attachment sites. Exemplifying this, Trichuris spp. inhabit a tunnel of epithelial cells within the host caecum and colon. A significant global burden of this infection persists, partly bec...
Article
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Findings from gut microbiome studies are strongly influenced by both experimental and analytical factors that can unintentionally bias their interpretation. Environment is also critical. Both co-housing and maternal effects are expected to affect microbiomes and have the potential to confound other manipulated factors, such as genetics. We therefor...
Article
Full-text available
Mucosal surfaces, as a first barrier with the environment are especially susceptible to damage from both pathogens and physical trauma. Thus, these sites require tightly regulated repair programs to maintain barrier function in the face of such insults. Barrier sites are also enriched for unconventional lymphocytes, which lack rearranged antigen re...
Article
Full-text available
Schistosomiasis is a major public health problem in Madagascar. The WHO recommends preventive chemotherapy by mass drug administration (MDA) with praziquantel as the primary approach to control Schistosoma mansoni –related morbidity in endemic populations, alongside complementary interventions such as health education. The impact of annual MDA and...
Preprint
Full-text available
Parasitic infections can be challenging to study, because light and electron microscopy are often limited in visualising complex and inaccessible attachment sites. Exemplifying this, Trichuris spp. inhabits a tunnel of epithelial cells within the host caecum and colon. A significant global burden of this infection persists partly because available...
Article
Full-text available
Background Dendritic cells (DCs) play a key role in shaping T cell responses. To do this, DCs must be able to migrate to the site of the infection and the lymph nodes to prime T cells and initiate the appropriate immune response. Integrins such as β 2 integrin play a key role in leukocyte adhesion, migration, and cell activation. However, the role...
Article
Full-text available
The parasitic nematode Trichuris trichiura is a significant burden on public health in developing countries, and currently available drugs exhibit a poor cure rate. Worms live within a specialised tunnel of host intestinal epithelial cells and have anterior-ventral projections of the cuticle termed “cuticular inflations”, which are thought to be in...
Article
Full-text available
Background Identifying the factors that contribute to chronicity in inflamed colitic tissue is not trivial. However, in mouse models of colitis, we can investigate at preclinical timepoints. We sought to validate murine Trichuris muris infection as a model for identification of factors that promote development of chronic colitis. Methods We compar...
Preprint
Gut microbiome analysis using 16S rRNA frequently focuses on summary statistics (e.g. diversity) or single taxonomic scales (e.g. Operational Taxonomic units, OTUs). This approach risks misinterpreting the phylogenetic or abundance scales of community differences (e.g. over-emphasising the role of single strains). We therefore constructed a 16S phy...
Article
Full-text available
The gut has the largest commensal bacterial population in the body and its composition can be impacted by host factors such as production of IgA. Eosinophils in the gut have been implicated in the production of anti‐bacterial factors and maintenance of IgA‐secreting plasma cells. We used an eosinophil‐deficient mouse (∆dblGATA‐1‐/‐) and littermate...
Preprint
Full-text available
Aims Identifying the factors that contribute to chronicity in inflamed colitic tissue is not trivial. However, in mouse models of colitis, we can investigate at preclinical timepoints. We sought to validate murine Trichuris muris infection as a model for identification of factors that promote development of chronic colitis. Methods We compared pre...
Article
Cerebral malaria (CM) is a serious neurological complication caused by Plasmodium falciparum infection. Currently, the only treatment for CM is the provision of antimalarial drugs; however, such treatment by itself often fails to prevent death or development of neurological sequelae. To identify potential improved treatments for CM, we performed a...
Article
Full-text available
Chronic wounds cause significant patient morbidity and mortality. A key factor in their etiology is microbial infection, yet skin host-microbiota interactions during wound repair remain poorly understood. Microbiome profiles of non-infected human chronic wounds are associated with subsequent healing outcome. Furthermore, poor clinical healing outco...
Article
Our previous work with immigrant communities identified a lack of awareness of infections such as gut worm (helminth) infections in their countries of origin, and a lack of English language skills around medical and scientific terms. Furthermore, people from minority ethnic and linguistic backgrounds participate significantly less in public engagem...
Article
Full-text available
Objective: Allergies are increasing, but the reasons for this are unclear. Although environmental factors are thought to be important, there is a lack of data on how they contribute to symptom development. To understand this relationship better, we need accurate data about both symptoms and environmental factors. Our objective here is to ascertain...
Article
Full-text available
Inflammatory bowel disease (IBD) is associated with altered microbiota composition and metabolism, but it is unclear whether these changes precede inflammation or are the result of it since current studies have mainly focused on changes after the onset of disease. We previously showed differences in mucus gut microbiota composition preceded colitis...
Article
Full-text available
X-ray micro-computed tomography ( μ CT) is a technique which can obtain three-dimensional images of a sample, including its internal structure, without the need for destructive sectioning. Here, we review the capability of the technique and examine its potential to provide novel insights into the lifestyles of parasites embedded within host tissue....
Article
Full-text available
Anthropogenic disturbance via resource acquisition, habitat fragmentation and climate change, amongst other factors, has led to catastrophic global biodiversity losses and species extinctions at an accelerating rate. Amphibians are currently one of the worst affected classes with at least a third of species categorised as being threatened with exti...
Data
ZSL, London Zoo herpetofauna species faecal samples. (PDF)
Data
Test for published[31] nematode universal primer efficacy on nematode tissue DNA and cross-reactivity on Platyhelminth tissue DNA. A: Nematode universal primers [31] designed for specific amplification of nematode DNA successfully amplified DNA from the nematodes T. muris (Tm) and T. spiralis (Ts). B, C: Nematode universal primers [31] also demonst...
Data
Test for published[19] nematode universal primer efficacy on nematode tissue DNA and cross-reactivity on Platyhelminth tissue DNA. Nematode universal primers [19] designed for specific amplification of nematode DNA successfully amplified DNA from the nematodes T. spiralis (Ts), A. lumbricoides (Al), N. brasiliensis (Nb), H. polygyrus (Hp) but not T...
Conference Paper
Background: In parallel with clinical care, it is equally important to empower young people with juvenile idiopathic arthritis (JIA) to develop self-efficacy skills, so that they can competently self-manage their health, particularly as they transition into adult health services. Capitalising on the popularity of technology, web-enabled tools repre...
Article
Full-text available
The skin microbiome exists in dynamic equilibrium with the host but when the skin is compromised, bacteria can colonise the wound and impair wound healing. Thus the interplay between normal skin-microbial interactions versus pathogenic-microbial interactions in wound repair is important. Bacteria are recognised by innate host pattern recognition re...
Article
Full-text available
Background: Inflammatory bowel disease (IBD) is associated with an inappropriate immune response to the gut microbiota. Notably, patients with IBD reportedly have alterations in fecal microbiota. However, the colonic microbiota occupies both the gut lumen and the mucus covering the epithelium. Thus, information about mucus-resident microbiota fail...
Article
Full-text available
Obesity impairs the relaxant capacity of adipose tissue surrounding the vasculature (PVAT) and has been implicated in resultant obesity-related hypertension and impaired glucose intolerance. Resident immune cells are thought to regulate adipocyte activity. We investigated the role of eosinophils in mediating normal PVAT function. Healthy PVAT elici...
Article
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Background Infection is a recognised risk factor for Alzheimer’s disease (AD) and can worsen symptoms in established disease. AD patients have higher rates of infection and are more likely to require hospital admissions due to infections than individuals without dementia. Infections have also been found to increase the risk of those over 84 years o...
Article
Full-text available
The murine model of experimental cerebral malaria (ECM) has been utilised extensively in recent years to study the pathogenesis of human cerebral malaria (HCM). However, it has been proposed that the aetiologies of ECM and HCM are distinct, and, consequently, no useful mechanistic insights into the pathogenesis of HCM can be obtained from studying...
Data
Immunofluorescent staining of intracerebral CD3+ T-cells in Pb ANKA and Pb NK65 infected mice and uninfected mice. C57/BL6 mice were infected with 1x104 Pb ANKA GFP or Pb NK65 GFP pRBCs (n = 5 / group), or left uninfected (n = 4). Mice were culled on d7 p.i. when Pb ANKA infected mice exhibited signs of late-stage ECM. Brains were removed from tran...
Data
Immunofluorescent staining of intracerebral cleaved caspase 3+ apoptotic cells in Pb ANKA and Pb NK65 infected mice and uninfected mice. C57/BL6 mice were infected with 1x104 Pb ANKA GFP or Pb NK65 GFP pRBCs (n = 5 / group), or left uninfected (n = 5). Mice were culled on d7 p.i. when Pb ANKA infected mice exhibited signs of late-stage ECM. Brains...
Data
Visualisation of life-cycle stages of intracerebral GFP+ parasites Pb ANKA infected mice. C57/BL6 mice were infected with 1x104 Pb ANKA GFP pRBCs (n = 3), and culled on d7 p.i. when infected mice exhibited signs of late-stage ECM. Brains were removed from transcardially perfused mice and single-cell suspensions generated for microscopic examination...
Data
Immunohistochemical staining of intracerebral IgG in Pb ANKA and Pb NK65 infected mice. C57/BL6 mice were infected with 1x104 Pb ANKA GFP or Pb NK65 GFP pRBCs (n = 5 / group), or left uninfected (n = 5). Mice were culled on d7 p.i. when Pb ANKA infected mice exhibited signs of late-stage ECM. Brains were removed from transcardially perfused mice an...
Data
Immunofluorescent staining and quantitation of CD31+ vasculature in the brains of Pb ANKA and Pb NK65 infected mice and uninfected mice. C57/BL6 mice were infected with 1x104 Pb ANKA GFP or Pb NK65 GFP pRBCs (n = 4 / group), or left uninfected (n = 4). Mice were culled on d7 p.i. when Pb ANKA infected mice exhibited signs of late-stage ECM. Brains...
Data
Immunofluorescent staining of intracerebral GFP+ parasites in Pb ANKA and Pb NK65 infected mice and uninfected mice. C57/BL6 mice were infected with 1x104 Pb ANKA GFP or Pb NK65 GFP pRBCs (n = 6 / group), or left uninfected (n = 5). Mice were culled on d7 p.i. when Pb ANKA infected mice exhibited signs of late-stage ECM. Brains were removed from tr...
Data
Immunofluorescent staining of Pb anti-sera+ parasite material in the brains of Pb ANKA and Pb NK65 infected mice. C57/BL6 mice were infected with 1x104 Pb ANKA GFP or Pb NK65 GFP pRBCs (n = 5 / group), and culled on d7 p.i. when Pb ANKA GFP infected mice exhibited signs of late-stage ECM. Brains were removed from transcardially perfused mice and ex...
Data
T-cells isolated from the whole brain of Pb ANKA infected mice at d7 p.i. are mainly CD8+. C57/BL6 mice were infected with 1x104 Pb ANKA GFP (n = 5). Mice were culled on d7 p.i. when Pb ANKA infected mice exhibited signs of late-stage ECM. Whole brains were removed from transcardially perfused mice and processed for flow cytometry. Representative f...
Data
Immunofluorescent staining detailing leukocyte composition of cerebral packed vessels in Pb ANKA infected mice, and histological staining and associated quantification of lymphocyte and pRBC co-localisation in Pb ANKA infected mice. C57/BL6 mice were infected with 1x104 Pb ANKA GFP pRBCs (n = 5 / group). Mice were culled on d7 p.i. when they exhibi...
Data
Immunohistochemical staining of intracerebral IgG in uninfected mice. Transcardially perfused brains were removed from uninfected mice and examined via immunohistochemistry for the presence of IgG. Representative images show the absence of endogenous IgG in the specified brain regions of uninfected mice. Scale bar: 75μm. (TIF)
Data
Immunofluorescent co-staining of NeuN+ neurons and CC3+ apoptotic cells in the brains of Pb ANKA and Pb NK65 infected mice. C57/BL6 mice were infected with 1x104 Pb ANKA GFP (n = 7) or Pb NK65 GFP pRBCs (n = 5). Mice were culled on d7 p.i. when Pb ANKA infected mice exhibited signs of late-stage ECM. Brains were removed from transcardially perfused...
Data
Correlation between different histopathological parameters in the brains of Pb ANKA infected mice. The regional correlation between any two histopathological parameters in the brains of mice infected with Pb ANKA and exhibiting symptoms of late-stage ECM (n = 4–8). Dots represent the mean of the group within a region. p: * ≤0.05, ** <0.005 (Spearma...
Data
Key detailing the ten assessed brain regions. Coronal brain sections stained by H&E (left panel) and with DAPI (right panel), dashed black line delineates each brain region assessed. *Caudoputamen is the rodent equivalent of the Striatum. (TIF)
Data
H&E staining of haemorrhage in Pb ANKA and Pb NK65 infected mice and uninfected mice. C57/BL6 mice were infected with 1x104 Pb ANKA GFP (n = 8) or Pb NK65 GFP pRBCs (n = 5), or left uninfected (n = 5). Mice were culled on d7 p.i. when Pb ANKA infected mice exhibited signs of late-stage ECM. Brains were removed from transcardially perfused mice and...
Data
Representative transmission electron micrographs of capillaries and small venules in the brains of Pb ANKA and Pb NK65 infected mice and uninfected mice. C57/BL6 mice were infected with 1x104 Pb ANKA GFP or Pb NK65 GFP pRBCs (n = 3 / group), or left uninfected (n = 3). Mice were culled on d7 p.i. when Pb ANKA infected mice exhibited signs of late-s...
Data
Generalised linear model analyses predicting the relative contribution of different histopathological parameters alone, and in combination, to vascular leakage in the brains of Pb ANKA infected mice. Table ranks models by R2 value. R2 value determines how closely observed data aligns with values predicted by generalised linear modelling. (TIF)
Poster
Full-text available
Background In parallel with clinical care, it is equally important to support young people with juvenile idiopathic arthritis (JIA) to develop self-efficacy skills, so that they can competently self-manage their health, particularly as they transition into adult health services. Capitalising on the popularity of technology, web-enabled tools repres...
Article
Full-text available
Background and aims: Arginase1 (Arg1), an M2 macrophage marker, plays a critical role in a number of immunological functions in macrophages, which are the main cell type facilitating atherosclerotic lesion development. Arg1 uses the substrate l-arginine to create l-ornithine, a precursor molecule required for collagen formation and vascular smooth...
Poster
Full-text available
Trichuriasis is a tropical disease that affects an estimated 500 million people worldwide, especially children in tropical regions. It is caused by a parasitic nematode, Trichuris trichiura (whipworm). Current drugs lack efficacy, and there is no vaccine. Adult worms live partially buried in the mucosal epithelium of the intestine of the host, whic...
Article
Full-text available
Infection and injury of the gut are associated with cell damage and release of molecules such as extracellular adenosine 5′-triphosphate (ATP), which is recognised by the purinergic P2X7 receptor (P2X7R). P2X7R is widely expressed in the gut by antigen-presenting cells (APCs) and epithelial cells, but the role of the P2X7R on epithelial cells is po...
Article
Full-text available
Background Eosinophils are innate immune cells present in the intestine during steady state conditions. An intestinal eosinophilia is a hallmark of many infections and an accumulation of eosinophils is also observed in the intestine during inflammatory disorders. Classically the function of eosinophils has been associated with tissue destruction, d...
Data
PubMed search terms used for each disease group and their approaches.DOI: http://dx.doi.org/10.7554/eLife.13615.004
Data
Rules used to identify the sex and age of experimental mouse models. DOI: http://dx.doi.org/10.7554/eLife.13615.014
Data
Example rules for identification of sex and age.DOI: http://dx.doi.org/10.7554/eLife.13615.005
Data
Corpus for assessing reporting of the sex and age of the mice. DOI: http://dx.doi.org/10.7554/eLife.13615.012
Data
(A) Set of articles for creating the text-mining rules. (B) Set of articles for finding the location of the mention of the sex and age of the mice. (C) Set of articles for enhancing the performance of the text-mining rules. (D) Set of articles for evaluating the text-mining system. DOI: http://dx.doi.org/10.7554/eLife.13615.013
Article
Wound infection is a major clinical problem, yet understanding of bacterial host interactions in the skin remains limited. Microbe-derived molecules, known as pathogen-associated molecular patterns, are recognised in barrier tissues by pattern-recognition receptors. In particular, the pathogen-associated molecular pattern, lipopolysaccharide (LPS),...
Article
Full-text available
A limited number of studies have investigated the potential of probiotics to promote wound healing in the digestive tract. The aim of the current investigation was to determine whether probiotic bacteria or their extracts could be beneficial in cutaneous wound healing. A keratinocyte monolayer scratch assay was used to assess re-epithelialization;...