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Shardendu K Singh

Shardendu K Singh
AeroFarms · R&D and Strategic Partnership

Ph.D.

About

46
Publications
11,167
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1,266
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Publications

Publications (46)
Article
Full-text available
The present study investigated the interactive effects of three environmental stress factors elevated CO2, temperature, and drought stress on soybean growth and yield. Experiments were conducted in the sunlit, controlled environment Soil–Plant–Atmosphere–Research chambers under two-level of irrigation (WW-well water and WS-water stress-35%WW) and C...
Article
Full-text available
Water stress (WS) and heat stress (HS) have a negative effect on soybean plant growth and crop productivity. Changes in the physiological characteristics, proteome, and specific metabolites investigated on molecular and cellular functions were studied in two soybean cultivars exposed to different heat and water stress conditions independently and i...
Article
Full-text available
Parental environments (PEs) affect seed quality and might alter the re-establishment of big bluestem grass due to impacts on seed germination. An in vitro study was conducted to quantify the temperature response of seed germination and its interaction with the PE in big bluestem. Seeds developed under eight PEs consisting of a combination of four d...
Article
Full-text available
Drought invokes a variety of metabolic alterations in plant leaves to cope with stress situations. To understand the effects of CO2 and drought stress for leaf metabolic changes in potato [Solanum tuberosum (L)], two contrasting potato cultivars Harley Blackwell (HB, an early maturing, newer cultivar) and Snowden (SD, an established, full-season cu...
Article
Full-text available
Impacts of potassium (K) deficiency and elevated carbon dioxide (eCO2) on seed constituents have rarely been explored in most crops including soybean. A controlled environment experiment was conducted with soybean grown under a sufficient (5.0 mM) and two deficient (0.50 and 0.02 mM) levels of K fertilization at ambient (aCO2) and eCO2 (400 and 800...
Article
Full-text available
An experiment was conducted to assess interactive effects of temperature (22, 26, 30, and 34°C daily mean T) and phosphorus (P) fertilization (sufficient, 0.5 mM, and deficient, 0.08 mM P) on soybean physiological traits. The P deficiency decreased leaf P concentration over approximately 50% across temperature regimes. However, a marked decrease in...
Article
Full-text available
Link to the paper: http://rdcu.be/HMZz :--------------------------------------------- Plants photosynthesis-related traits are co-regulated to capture light and CO2 to optimize the rate of CO2 assimilation (A). The rising CO2 often benefits, but potassium (K) deficiency adversely affects A that contributes to the majority of plant biomass. To eva...
Article
Full-text available
In nature, crops such as soybean are concurrently exposed to temperature (T) stress and phosphorus (P) deficiency. However, there is a lack of reports regarding soybean response to T × P interaction. To fill in this knowledge-gap, soybean was grown at four daily mean T of 22, 26, 30, and 34°C (moderately low, optimum, moderately high, and high temp...
Article
Analysis of uppermost fully expanded leaves is useful to detect a deficiency of mineral nutrients such as phosphorus (P) and potassium (K) in soybean. Although, the leaf P or K status aids in fertilizer management, information on nutrient seasonal relationships with growth and yield traits at maturity are limited. To investigate this, soybean was g...
Article
Remote estimation of leaf nitrogen (N) or pigments through hyperspectral reflectance offers an opportunity to non-destructively diagnose plant N status. Two sweet sorghum (Sorghum bicolor [L.] Moench) cultivars (Top 76-6 and Dale) were grown with 0, 56, 112, 168, and 224 kg N ha⁻¹ in 2009 and 2010. Reflectance measurements were coupled with plant h...
Article
Full-text available
Elevated carbon dioxide (eCO2) often enhances plant photosynthesis, growth, and productivity. However, under nutrient-limited conditions the beneficial effects of high CO2 are often diminished. To evaluate the combined effects of potassium (K) deficiency and eCO2 on soybean photosynthesis, growth, biomass partitioning, and yields, plants were grown...
Article
Full-text available
A detailed investigation of the concentration (e.g. mg g-1 seed) and total yield (e.g. g plant-1) of seed mineral elements and metabolic profile under phosphorus (P) starvation at ambient (aCO2) and elevated carbon dioxide (eCO2) in soybean is limited. Soybean plants were grown in a controlled environment at either sufficient (0.50 mM P, control) o...
Conference Paper
Full-text available
Chlorophyll is a major component of chloroplasts and a better understanding of the genetic basis of chlorophyll content and concentration in soybean [Glycine max (L.) Merr.] might contribute to improving photosynthetic capacity and yield in regions with adverse environmental conditions. A collection of 332 diverse soybean genotypes were grown in tw...
Article
To assess the relationship between chlorophyll (Chl) fluorescence (CF) and photosynthetic pigments, soybean was grown under varying phosphorus (P) nutrition at ambient and elevated CO2 (EC). The EC stimulated, but P deficiency decreased plant height, node numbers, and leaf area concomitantly with the rates of stem elongation, node addition, and lea...
Article
To evaluate the combined effect of temperature and CO2 on photosynthetic processes, leaf metabolites and growth, soybean was grown under a controlled environment at low (22/18°C, LT), optimum (28/24°C, OT) and high (36/32°C HT) temperatures under ambient (400μmolmol(-1); aCO2) or elevated (800μmolmol(-1); eCO2) CO2 concentrations during the reprodu...
Article
Full-text available
Background Chlorophyll is a major component of chloroplasts and a better understanding of the genetic basis of chlorophyll in soybean [Glycine max (L.) Merr.] might contribute to improving photosynthetic capacity and yield in regions with adverse environmental conditions. A collection of 332 diverse soybean genotypes were grown in 2 years (2009 and...
Article
Soybean plants were grown to maturity in controlled environment chambers and at the onset of flowering three temperature treatments were imposed that provided optimum [28/24 °C], low [22/18 °C] or high [36/32 °C] chamber air temperatures. In addition, plants were treated continuously with either 400 or 800 μmol mol−1 CO2. Seeds were harvested at 42...
Article
Despite the development of various methods, rapid mesophyll conductance (gm) estimation for large number of sample is still a daunting challenge. Although, accurate estimation of gm is critical to partition photosynthetic limitations by stomatal (Ls) and mesophyll (Lm) conductance and by photo-biochemical (Lb) processes, impact of various gm estima...
Chapter
Full-text available
In natural settings such as under field conditions, the plant-available soil nutrients in conjunction with other environmental factors such as solar radiation, temperature, precipitation, and atmospheric carbon dioxide (CO2) concentration determine crop adaptation and productivity. Therefore, crop success depends on the intricate balance among thes...
Conference Paper
Full-text available
Photosynthetic capacity is important for total biomass production and ultimately grain yield. The amount of solar radiation that is absorbed by a leaf is closely related to its chlorophyll concentration, which generally is positively related with photosynthetic rate. A better understanding of the genetic basis of chlorophyll concentration in soybea...
Article
Full-text available
Nitrogen is a primary plant nutrient that plays a major role in achieving maximum economic yield. Insufficient availability most often limits soybean [Glycine max (L.) Merr.] crop growth. Symbiotic N2 fixation in soybean is highly sensitive to limited water availability, and breeding for reduced N2 fixation sensitivity to drought is considered an i...
Article
Full-text available
Nitrogen is a primary plant nutrient that plays a major role in achieving maximum economic yield. Insufficient availability most often limits soybean [Glycine max (L.) Merr.] crop growth. Symbiotic N2 fixation in soybean is highly sensitive to limited water availability, and breeding for reduced N2 fixation sensitivity to drought is considered an i...
Article
Full-text available
Ureides are the N-rich products of N-fixation that are transported from soybean nodules to the shoot. Ureides are known to accumulate in leaves in response to water-deficit stress and this has been used to identify genotypes with reduced N-fixation sensitivity to drought. Our objectives in this research were to determine shoot ureide concentrations...
Article
Full-text available
Carotenoids are organic pigments that are produced predominantly by photosynthetic organisms and provide antioxidant activity to a wide variety of plants, animals, bacteria, and fungi. The carotenoid biosynthetic pathway is highly conserved in plants and occurs mostly in chromoplasts and chloroplasts. Leaf carotenoids play important photoprotective...
Conference Paper
Full-text available
Carotenoid biosynthesis occurs mostly in chromoplasts and chloroplasts, and its biosynthetic pathway is highly conserved in plants. Leaf carotenoids play an important photo-protective role during the process of photosynthesis and targeted selection of leaf carotenoids may offer avenues for improved abiotic stress tolerance. A collection of 332 geno...
Article
Full-text available
Key message Using genome-wide association studies, 39 SNP markers likely tagging 21 different loci for carbon isotope ratio (δ 13 C) were identified in soybean. Abstract Water deficit stress is a major factor limiting soybean [Glycine max (L.) Merr.] yield. Soybean genotypes with improved water use efficiency (WUE) may be used to develop culti...
Article
To examine the combined effects of phosphorus (P) nutrition and CO2 on photosynthesis, chlorophyll fluorescence (CF), and nutrient utilization and uptake, two controlled-environment experiments were conducted using 0.01, 0.05 and 0.20 mM external phosphate each at ambient and elevated CO2 (aCO2: 400 and eCO2: 800 µmol mol−1, respectively). The CF p...
Presentation
Analysis of Leaf Proteome to Determine Possible Cross-Tolerance to Abiotic Stresses in Soybean Cultivars (Submission 183) Ramesh Katam1, Raja Reddy Kambham2, Shardendu K Singh2, Nitya S Murty3 1Florida A&M University, United States, 2Mississippi State University, United States, 3Emory University, United States Soybean (Glycine max (L.) Merr.) is a...
Article
Leaf and canopy characteristics influence light interception and photosynthesis, which ultimately contribute to total biomass production and crop yield. Inexpensive hand-held digital cameras may provide an alternative to time-consuming destructive sampling methods used to assess these traits. The objective of this study was to test the use of commo...
Article
Full-text available
Plant response to the combination of two or more abiotic stresses is different than its response to the same stresses singly. The response of maize (Zea mays L.) photosynthesis, growth, and development processes were examined under sunlit plant growth chambers at three levels of each day/night temperatures (24/16°C, 30/22°C, and 36/28°C) and UV-B r...
Article
Plant mineral nutrients such as phosphorus may exert major control on crop responses to the rising atmospheric carbon dioxide (CO2) concentrations. To evaluate the growth, nutrient dynamics, and efficiency responses to CO2 and phosphorus nutrition, soybean (Glycine max (L.) Merr.) was grown in controlled environment growth chambers with sufficient...
Article
Phosphorous deficiency in soil limits crop growth and productivity in the majority of arable lands worldwide and may moderate the growth enhancement effect of rising atmospheric carbon dioxide (CO2) concentration. To evaluate the interactive effect of these two factors on cotton (Gossypium hirsutum) growth and physiology, plants were grown in contr...
Conference Paper
Full-text available
Water deficit stress continues to be one of the major factors limiting soybean yield [Glycine max (L.) Merr.], the world’s leading economic oilseed crop. One of the major benefits of legumes in agriculture is their capacity to symbiotically fix atmospheric nitrogen, thus reducing the need to use nitrogen fertilizers. In drought conditions, soybean...
Article
Full-text available
To understand the consequences of rising levels of ultraviolet-B (UV-B) radiation on corn (Zea mays L.), two experiments were conducted using sunlit growth chambers at a wide range of UV-B radiation levels. Corn hybrids, Terral-2100 and DKC 65-44, were grown in 2003 and 2008, respectively, at four UV-B levels (0, 5, 10, and 15 kJ m(-2) d(-1)) at 30...
Article
Accurate prediction of leaf pigments from spectral reflectance is important because it allows non-destructive, rapid assessment of crop-N status under field conditions. Canopy reflectance and leaf pigments (chlorophyll and carotenoids concentrations) were measured on 385 field-grown soybean genotypes during flowering and seed development stages eac...
Conference Paper
Full-text available
Water deficit stress continues to be one of the major factors limiting soybean yield [Glycine max (L.) Merr.]. Soybean genotypes with increased water use efficiency (WUE) may be used to develop cultivars with increased yield under drought. Carbon isotope discrimination has been used as a simple and reliable measure for WUE in diverse crops with C3...
Article
â–º Utility of restricted rooting depths system was tested for water deficit study in soybean. â–º Rooting depth influenced seed quality regardless of amount of seasonal rainfall. â–º This system creates water deficit even in regions with significant rainfall. â–º It provides an alternative way to manipulate soil water for water deficit study in cr...
Article
Drought is the major abiotic stress factor that causes extensive losses to agriculture production worldwide. The objective of this study was to evaluate the dynamics of photosynthesis and water-use efficiency parameters in 15 cowpea genotypes under well-watered and drought condition. Photosynthesis (A) and chlorophyll fluorescence (Fv'/Fm') decline...
Article
Full-text available
The carbon dioxide concentration [CO(2)], temperature and ultraviolet B radiation (UVB) are concomitant factors projected to change in the future environment, and their possible interactions are of significant interest to agriculture. The objectives of this study were to evaluate interactive effects of atmospheric [CO(2)], temperature, and UVB radi...
Chapter
Full-text available
Crops are often exposed to multiple factors of climate change including: (1) enhanced ultraviolet-B (UV-B) radiation, (2) elevated carbon dioxide concentrations (CO2), and (3) episodes of elevated temperatures and water stress during critical stages of crop development. Our understanding of crop responses to individual climate change stress factors...
Article
Current and projected increases in ultraviolet-B (UV-B) radiation (280–320 nm) may alter crop production. An experiment was conducted to study the influence of UV-B radiation on photosynthetic CO2 assimilation, photosystem II (PSII) photochemistry, pigments, UV-B absorbing compounds, vegetative and reproductive growth responses of three cowpea cult...
Article
The current and projected terrestrial ultraviolet-B (UV-B) radiation affects growth and reproductive potential of many crops. Cowpea (Vigna unguiculata [L.] Walp.), mostly grown in tropical and sub-tropical regions may already be experiencing critical doses of UV-B radiation due to a thinner ozone column in those regions. Better understanding of ge...
Article
Full-text available
Winter-grown canola (Brassica napus L.) production is limited mostly by frost and winter kill in the southern canola-growing regions of the United States. Tolerance to cold and heat were assessed by studying percentage of pollen viability (PV), in vitro pollen germination (PG) and pollen tube length (PTL) for 12 field-grown cultivars. Freshly colle...

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