Sharad Gupta

Sharad Gupta
Indian Institute of Technology Gandhinagar · Biological engineering

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40
Publications
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Introduction
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Publications

Publications (40)
Article
Full-text available
Alpha‐Synuclein (α‐Syn), an intrinsically disordered protein (IDP), is associated with neurodegenerative disorders, including Parkinson's disease (PD) or other α‐synucleinopathies. Recent investigations propose the transmission of α‐Syn protein fibrils, in a prion‐like manner, by entering proximal cells to seed further fibrillization in PD. Despite...
Article
Full-text available
Designing programmable biomaterials that could act as extracellular matrices and permit functionalization is a current need for tissue engineering advancement. DNA based hydrogels are gaining significant attention owing to their self-assembling properties, biocompatibility, chemical robustness and low batch to batch variability. The real potential...
Article
Full-text available
Tau protein is found abundantly in neurofibrillary tangles in Alzheimer’s disease. The longest human tau isoform (2N4R) has 44 lysine residues. Several lysine‐based post‐translational modifications (PTMs) such as glycation, acetylation, ubiquitination, and sumoylation have been implicated not only in Alzheimer's disease but also in other tauopathie...
Article
The synthesis and biological studies of BODIPY-GPR peptide conjugate (BD-2) are reported. As compared to the parent BODIPY (BD-1), the peptide linked BD-2showed blue shifted absorption and emission with excellent Stokes shift of 201 nm. Molecular docking studies on EGFR protein kinase indicated very efficient binding affinity of BD-2 as compared to...
Article
Full-text available
Parkinson's disease is the second most prevalent neurodegenerative disease. The loss of dopaminergic neurons in the substantia nigra is one of the pathological hallmarks of PD. PD also belongs to the class of neurodegenerative disease known as 'Synucleinopathies' as α-synuclein is responsible for disease development. The presence of aggregated α-sy...
Article
Full-text available
We demonstrate a benign and straightforward method to modify the chitosan (CH) by carbamoylation. The free amines on CH are converted into carbamyl functionalities by reacting with potassium cyanate (KCNO). One wt% CH solution, when reacted with KCNO  0.1 M, leads to the sol-gel transition of CH through the hydrogen bonding to form carbamoylated c...
Article
Full-text available
Intra-neuronal misfolding of monomeric tau protein to toxic β-sheet rich neurofibrillary tangles is a hallmark of Alzheimer’s disease (AD). Tau pathology correlates not only with progressive dementia but also with microglia-mediated inflammation in AD. Amyloid-beta (Aβ), another pathogenic peptide involved in AD, has been shown to activate NLRP3 in...
Article
The direct write printing method has gained popularity in synthesizing scaffolds for tissue engineering. To achieve an excellent printability of scaffolds, a thorough evaluation of rheological properties is required. We report the synthesis, characterization, rheology, and direct-write printing of chitosan - graphene oxide (CH - GO) nanocomposite h...
Article
Full-text available
Multiple endocytic pathways operate on the plasma membrane of cells at any moment with diverse but specific cellular functions. Knowledge of uptake of synthetic nanoparticles and ligands with respect to endocytic pathways is crucial to device the appropriate ligands for therapeutic delivery into differentiated neurons for targeting neuronal disease...
Article
Full-text available
A series of triazole-based compounds was synthesized using a click chemistry approach and were evaluated for the inhibition of α-synuclein (α-syn) fibrillogenesis and its disaggregation. Compounds Tr3, Tr7, Tr12, Tr15, and Tr16 exhibited good effect in inhibiting α-syn fibrillogenesis confirmed by Thioflavin-T assay and fluorescence microscopy and...
Article
Full-text available
The broad area of neuroscience has witnessed an increasing exploitation of a variety of synthetic biomaterials with controlled nanosized features. Different bionanomaterials offer very peculiar physicochemical and biochemcial properties contributing to the development of novel imaging devices toward imaging the brain, or as smartly functionalized s...
Article
Aggregation of α-synuclein (α-syn) is one of the central hypotheses for Parkinson’s disease (PD), therefore, its inhibition and disaggregation is an optimistic approach for the treatment of PD. Here, we report design, synthesis and in-vitro efficacy studies of a series of diphenyl triazine hybrids as potential inhibitors of α-syn fibrillogenesis. F...
Article
The present study demonstrates potential utilization of lime-stabilized sewage sludge (SS) for construction purposes. SS samples were constituted using 0, 2, 4, 6 and 8% of lime by weight of the SS and cured for 7, 14 and 28 d. Stability of the lime-modified SS was evaluated through determination of geotechnical, microstructural and mineralogical p...
Article
Polyphenols have been suggested as potential therapeutic agents for the treatment of amyloidogenic diseases. In this work, we evaluate quercetin-rich onion extract for its ability to inhibit tau fibrillization. Considering the presence of polyphenols in multiple glycosidic and aglycosidic forms, a nanobiocatalyst-mediated approach has been used to...
Article
Background: Carbamylation is a non-enzymatic post-translational modification (PTM), which involves the covalent modification of N-terminus of protein or ε-amino group of Lys. The role of carbamylation in several age-related disorders is well documented, however, the relationship between carbamylation and neurodegenerative disorders including Alzhe...
Poster
Tau is an intrinsically disordered protein and has a significant role maintaining the structure and stability of axonal microtubules. In Alzheimer’s disease (AD) and related disorders, tau protein may undergo biochemical and structural changes to form intraneuronal tangles of paired helical filaments (PHF) which progressively result in neuronal dea...
Article
We study the adsorption of protein ovalbumin (OVA) at Corn Oil (CO), Soybean Oil (SBO), Olive Oil (OO) and water interfaces along with the emulsification of these oils in water. The dynamic interfacial tension (IFT) measurements show a reduction in IFT in the order SBO-water ∼CO-water>OO-water, with OVA adsorption being dominated by the free diffus...
Article
Tau is an intrinsically disordered protein responsible for maintaining the structure and stability of axonal microtubules. However, in certain disease conditions including Alzheimer's disease, tau protein may undergo biochemical and structural changes to form intracellular aggregates. Since tau is a proline and arginine rich eukaryotic protein, het...
Article
New synthetic strategy to synthesize α-methyl BODIPY derivatives from dipyrromethanes is reported. The method involves regioselective formylation of dipyrromethane followed by modified Wolff-Kishner reduction. The photophysical, electrochemical and computational studies of α-methyl BODIPY derivatives have been investigated in detail. The α-methyl B...
Poster
Validating heparin induced tau in vitro aggregation models
Article
We study the influence of phosphorylation on the foamability and stability of Bovine Serum Albumin (BSA), phosphorylated BSA (pBSA) and citrus peel pectin (CPP) mixed foams as a function of BSA – CPP, pBSA – CPP mixing ratios, at a fixed solution pH of 4.5. Our results show that pBSA solutions result in more stable foams as compared to their BSA co...
Article
In Solid Phase Peptide Synthesis (SPPS), contamination with deletion sequences which often co-elute with the target peptide continues to be a major challenge as these impurities can significantly affect the target peptide’s properties. Here, we report an efficient Fmoc-deprotection solution containing piperazine and DBU which can cause complete rem...
Conference Paper
BACKGROUND The microtubule-associated protein tau is abundantly expressed in neurons, and plays a key role in regulating microtubule stability. Along with Abeta amyloids, taupathy is one of the hallmark of Alzheimer’s Disease. Short peptide fragments present in the repeat domains of Tau protein are primary responsible for the onset of protein aggre...
Article
Many neurodegenerative diseases are associated with deposits of aggregated protein in the brain. The molecular pathways through which soluble proteins misfold to form amyloids and large protein aggregates often include diverse oligomeric species, only some of which progress to the amyloid state. Here we show that prefibrillar huntingtin (HTT) oligo...
Conference Paper
Protein misfolding and aggregation has been implicated in the pathogenesis of several neurodegenerative disorders including Alzheimer’s, Parkinson’s and Huntington’s diseases. A prion like propagation mechanism has been widely proposed and the emerging consensus is that the nano-sized multimers called oligomers, which are formed very early in the a...
Conference Paper
Improper and unrestricted use of antibiotics has led to resistance among microbial populations resulting in complex and longer treatment strategies for easily curable diseases. Microorganisms evolve rapidly by mutation or by acquiring resistance gene as an effect of conjugation, transformation & transduction. An incomplete course of antibiotic or p...
Article
Full-text available
Huntington disease (HD) is one of several fatal neurodegenerative disorders associated with misfolded proteins. Here, we report a novel method for the sensitive detection of misfolded huntingtin (HTT) isolated from the brains of transgenic (Tg) mouse models of HD and humans with HD using an amyloid seeding assay (ASA), which is based on the propens...
Article
The synthesis of the first functionalized bis-amino acid monomer proAc(2S3S4R) 1 that carries an acetyl side chain is presented. This monomer was incorporated into oligomer 3 and the solution phase structure was determined by using two-dimensional nuclear magnetic resonance. The solution structure confirmed the intended connectivity and stereochemi...
Article
Proteins catalyze specific chemical reactions and carry out highly selective molecular recognition because they adopt well-defined three-dimensional structures and position chemically reactive functional groups in specific constellations. Proteins attain these well-defined structures through the complex process of protein folding. We seek to emulat...
Article
We have developed second-generation monomers 1 and 2 and improved conditions for rapidly and simultaneously closing multiple diketopiperazines on solid support. These new conditions involve either the microwave heating of a suspension of solid-supported amino-tetrafluoropropyl esters in acetic acid/triethylamine catalyst solution or continuous flow...
Article
[reaction: see text] The synthesis of a new pipecolic acid-based bis-amino acid building block 1 (and 2) is presented. Assembly of this monomer into a spiro ladder oligomer 3 utilizing solid-phase synthesis followed by in situ activation by dicyclohexylcarbodiimide and N-hydroxysuccinimide has been demonstrated. The structure of oligomer 3, determi...

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