Shahram Ghanaati

Medical Center of the Goethe University of Frankfurt/Main · Department for Oral Cranio-Maxillofacial and Facial Plastic Surgery

Background: Evaluation of impact of adjuvant radiation therapy (RT) in patients with oral squamous cell carcinoma of the oral cavity/oropharynx (OSCC) of up to 4 cm (pT1/pT2) and solitary ipsilateral lymph node metastasis (pN1). A non-irradiated group with clinical follow-up was chosen for control, and survival and quality of life (QL) were compare...

IntroductionThe aim of the study was to evaluate the accuracy of MRI and CT with regard to the detection of lymph node metastases based on the data of specific patients with OSCC who received bilateral neck dissection.Materials and methodsIn a retrospective analysis from 01/2014 to 12/2020 patients who underwent primary tumor resection and bilatera...

Objective Tumor resection and reconstruction in patients with oral squamous cell carcinoma often involves free flaps followed by radiation therapy. Volume loss of free flaps is common following transplantation and radiation therapy. Therefore, this study investigates the volume loss of free radial forearm flaps in the field of craniomaxillofacial s...

Background The incidence of pyogenic spinal infection has increased in recent years. In addition to treating the spinal infection, optimal care also includes identifying the source of the pyogenic spinal infection and the presence of other infections. The aim of this study is to elucidate the prevalence of oral cavity infection (OCI) within this Pa...

Systematic evaluations regarding the influence of PRF in ridge sealing are still lacking. To the best of our knowledge, this is the first systemic randomized, controlled, clinical approach dealing with the potential of a systematic applied solid PRF on soft tissue socket healing of molar and premolar extraction sockets with evaluation for up to 90...

Osteoradionecrosis (ORN) of the jaws is an uncommon complication of radiation therapy that seriously affects the oral and maxillofacial region. Management of ORN is intrinsically difficult and treatment effects are unpredictable. ORN can be treated with pentoxifylline/tocopherol and autologous platelet concentrates to promote wound healing. Further...

Zusammenfassung Hintergrund Patienten mit einem Kopf-Hals-Tumor besitzen ein hohes Risiko, peri- bzw. posttherapeutisch eine Mangelernährung zu entwickeln, die den onkologischen Therapieerfolg entscheidend beeinflussen kann. Prätherapeutisch ist hierüber wenig bekannt. Daher ist das Ziel der vorliegenden Studie die prätherapeutische systematische E...

Objective Tumor resection and reconstruction in patients with oral squamous cell carcinoma often involves free flaps followed by radiation therapy. Volume loss of free flaps is common following transplantation and radiation therapy. Therefore, this study investigates the volume loss of free radial forearm flaps in the field of craniomaxillo- facial...

Introduction: Treatment of extended defects after tumor resection in oral, maxillofacial, and facial surgery (craniomaxillofacial) is usually performed by free microvascular flaps. Evaluation of flap survival is crucial, especially in the first hours after insertion and connection. For flap evaluation various invasive and noninvasive methods have b...

•Consider tissue expanders for challenging DBS cases in PD patients with hardware erosion.•Placement of tissue expander is essential in planning the reconstruction.•MRI-compatibility of the tissue expander is paramount for shortening the total duration of anesthesia.•Role of routine skin biopsies to identify PD patients at additional risk for devel...

Aim: The purpose of this study was to obtain data concerning growth factor release within liquid and solid platelet-rich fibrin (PRF) matrices and to estimate the amount of potential interindividual variations as a basis for further preclinical and clinical trials. Therefore, we aimed to determine possible differences in the release of growth facto...

Introduction: Treatment of extended defects after tumor resection in oral, maxillofacial, and facial surgery (craniomaxillofacial) is usually performed by free microvascular flaps. Evaluation of flap survival is crucial, especially in the first hours after insertion and connection. For flap evaluation various invasive and noninvasive methods have...

AimThe primary aim of this study was to analyze frequency and characteristics of combined facial and peripheral trauma with consecutive hospitalization and treatment.Materials and methodsThe study included all patients with concomitant orthopedic-traumatolgical (OT) and craniomaxillofacial (CMF) injuries admitted to our level I trauma center in 201...

The combination of histological and biomolecular analyses provides deep understanding of different biological processes and is of high interest for basic and applied research. However, the available analytical methods are still limited, especially when considering bone samples. This study compared different fixation media to identify a sufficient a...

Introduction: Tumor resection combined with neck dissection (ND) or radiotherapy are established methods for the treatment of patients with oral squamous cell carcinoma (OSCC). However, the extent of ND can lead to postoperative complications. Therefore, for the first time, this study aims to identify lymph node involvement in OSCC performed in a...

Zusammenfassung Hintergrund Patienten mit einem Kopf-Hals-Tumor besitzen ein hohes Risiko, peri- bzw. posttherapeutisch eine Mangelernährung zu entwickeln, die den onkologischen Therapieerfolg entscheidend beeinflussen kann. Prätherapeutisch ist hierüber wenig bekannt. Daher ist das Ziel der vorliegenden Studie die prätherapeutische systematische E...

Background A profound understanding of the evolution and anatomy of the viscero- and neurocranium is quintessentially important for orthodontists. This particularly alludes to structures, which are directly targeted by orthodontic therapy such as the maxilla and the mid-palatal suture. The anatomy of the mid-palatal suture of toothed individuals is...

Aims: The objective of the present case series is to report on the rationale, surgical technique and outcome of a protocol for peri-implant mucosal phenotype modification therapy, referred to as "fibrin immobilization vestibular extension (FIVE)". Material and methods: The protocol utilized entailed apical positioning and stabilization of peri-i...

Background To study neoadjuvant chemoradiotherapy (nCRT) and potential predictive factors for response in locally advanced oral cavity cancer (LA-OCC).Methods The INVERT trial is an ongoing single-center, prospective phase 2, proof-of-principle trial. Operable patients with stage III-IVA squamous cell carcinomas of the oral cavity were eligible and...

Objectives: This study evaluated the cellular response of primary osteoblasts exposed to two different presentations of a low-temperature non-sintered deproteinized bovine bone matrix (DBBM). Materials and methods: Six different baths of a commercially available DBBM block (Bonefill® Porous Block) and one of DBBM granule (Bonefill® Porous) were eva...

Evaporation of phosphate species during thermal treatment (> 400°C) of calcium phosphates leads to the formation of an alkaline layer on their surface. The aim of this study was to evaluate the hypothe- sis that the biological response of thermally treated calcium phosphates is modified by the presence of such an alkaline layer on their surface. Fo...

Introduction: The diagnosis and therapy of oral squamous cell carcinoma (OSCC) in Germany is according to guidelines and relies on interdisciplinary board meetings. Standard examination techniques are computed tomography (CT) and magnet resonance imaging (MRI). These technologies are used as objective tools for serial presentation in an oncologic...

Objectives To assess the influence of antiresorptive/antiangiogenic therapy on the spreading of peri-implant infections in the pharyngeal region. Material and methods This analysis was based on tissue biopsies obtained from a total of twenty-five albino rats having either received (1) amino-bisphosphonate (Zoledronate) (Zo) ( n =4), (2) RANKL inhi...

Purpose To address the focused question: in patients with freshly extracted teeth, what is the efficacy of platelet-rich fibrin (PRF) in the prevention of pain and the regeneration of soft tissue and bone compared to the respective control without PRF treatment? Methods After an electronic data search in PubMed database, the Web of Knowledge of Th...

Background Salivary gland carcinomas (SGC) cover a heterogeneous group of malignancies with a lack of data of high-level evidence. Methods Clinical data of 127 patients treated for SGC at a university cancer center between 2002 and 2017 were analyzed retrospectively. The association of clinicopathological characteristics, treatment modalities, adv...

Adsorbate biohybrids for BMP-2 delivery based on electrospun PDLLA-nanofiber fleeces loaded with 0.6 μg/cm2 rhBMP-2COL (E. coli) strongly induced microvessel- like structures when incubated for 14 days in cocultures of human outgrowth endothelial cells (OEC) together with human primary osteoblasts (pOB). Higher loaded rhBMP-2COL biohybrids (4.6 μg/...

Background Deep surgical site infections (dSSIs) after instrumented spinal surgery pose major therapeutic challenges. Standard treatment involves surgical debridement, wound drainage, and long-term antibiotic administration. Autologous platelet-rich fibrin (PRF) constitutes a biomaterial obtained from patients’ own blood that contains leukocytes, c...

Platelet rich fibrin (PRF) is an autologous blood concentrate, that supports tissue regeneration. The effect of the centrifuge rotor angle in the fabrication of PRF is still not fully elucidated. The hypothesis of this study is: When applying the same g-force {relative centrifugal force (RCF)} and centrifugation time, PRF components and bioactivity...

Multinucleated giant cells (MNGCs) are frequently observed in the implantation areas of different biomaterials. The main aim of the present study was to analyze the long-term polarization pattern of the pro- and anti-inflammatory phenotypes of macrophages and MNGCs for 180 days to better understand their role in the success or failure of biomateria...

Zusammenfassung Hintergrund Dysphagie bildet eine häufige postoperative Funktionsbeeinträchtigung bei Kopf-Hals-Tumorpatienten. Diese kann in Aspiration bzw. Penetration sowie Oralisierungseinschränkung resultieren und ist dadurch häufig versorgungsrelevant. In dieser Studie wurden die Prävalenz einer postoperativen Dysphagie und der Zusammenhang z...

Sulfated glycosaminoglycans (sGAG) show interaction with biological mediator proteins. Although collagen-based biomaterials are widely used in clinics, their combination with high-sulfated hyaluronan (sHA3) is unexplored. This study aims to functionalize a collagen-based scaffold (Mucograft®) with sHA3 via electrostatic (sHA3/PBS) or covalent bindi...

Background The high-oblique sagittal osteotomy (HOSO) is an alternative to a bilateral sagittal split osteotomy (BSSO). Due to its novelty, there are no long-term studies which have focused on describing the incidence and type of complications encountered in the post-operative follow-up. The aim of this retrospective study is to analyze patients op...

Introduction: E-Scooters are trending as an individual transport vehicle with electric drive in Germany. But there is less data about the frequency, demographic details and possible injuries and therapy needs in context with E-scooter accidents. Therefore, the primary aim of this study was to analyze the frequency and the characteristics of facial...

Objectives The aim of the present study was to characterize the cellular reaction to a xenogeneic resorbable collagen membrane of porcine origin using a subcutaneous implantation model in Wistar rats over 30 days.Materials and methodsEx vivo, liquid platelet-rich fibrin (PRF), a leukocyte and platelet-rich cell suspension, was used to evaluate the...

Background The rate of loco-regional recurrences for locally advanced head and neck squamous cell carcinoma (HNSCC) following standard treatment reaches up to 50%, accompanied by a probability of 20% to develop a second primary tumor in the head and neck region. Methods Ten patients with inoperable, in-field recurrence of HNSCC following previous...

Introduction: Resorbable synthetic scaffolds are promising for different indications, especially in the context of bone regeneration. However, they require additional biological components to enhance their osteogenic potential. In addition to different cell types, autologous blood-derived matrices offer many advantages to enhance the regenerative c...

This study evaluated the impact of rotor angle and time of storage after centrifugation on the in vitro biological properties of platelet-rich fibrin (PRF) membranes. Blood samples (n = 9) were processed with a vertical fixed-angle (V) or a swing-out horizontal (H) centrifuge, with 20-60 min of sample storage after centrifugation. Leukocytes, plate...

Evaporation of phosphate species during thermal treatment (> 400 °C) of calcium phosphates leads to the formation of an alkaline layer on their surface. The aim of this study was to evaluate the hypothesis that the biological response of thermally treated calcium phosphates is modified by the presence of such an alkaline layer on their surface. For...

Objectives Aim of the study was to evaluate the prognostic impact of CD8-positive (CD8⁺) tumour-infiltrating lymphocytes (TILs) and PD-L1 expression on the outcome of patients with malignant salivary gland neoplasms. Materials and methods Formalin-fixed, paraffin-embedded tissue samples and clinicopathological data from patients treated for saliva...

Background The present study evaluated the cellular tissue reaction of two equine-derived collagen hemostatic sponges (E-CHS), which differed in thickness after pressing, over 30 days in vivo. The inflammatory response during physiological wound healing in sham-operated animals was used as control group. Material and methods First, the E-CHS was p...

In vitro tissue engineered cell culture models are an essential instrument to investigate physiological and pathophysiological wound healing mechanisms and to evaluate new beneficial wound dressing materials and therapeutics to identify possible drug targets and to improve regeneration processes in non-healing and chronic wounds. In this study, the...

The permeability and inflammatory tissue reaction to Mucomaix® matrix (MM), a non- cross-linked collagen-based matrix was evaluated in both ex vivo and in vivo settings. Liquid platelet rich fibrin (PRF), a blood concentrate system, was used to assess its capacity to absorb human proteins and interact with blood cells ex vivo. In the in vivo aspect...

Background and Objective Macrophages’ cytokine expression and polarization play a substantial role in the host's “destructive” inflammatory response to periodontal and peri‐implant pathogens. This study aimed to evaluate cell viability, anti‐inflammatory activity, and macrophage polarization properties of different cranberry concentrates. Methods...

Background Watertight closure of the dura mater is fundamental in neurosurgery. Besides the classical suturing techniques, a variety of biomaterials have been proposed as sealants. Platelet rich fibrin (PRF) is an autologous biomaterial which can readily be obtained through low-speed centrifugation of patient’s own blood. It is rich in fibrin, grow...

Background: Oral cancer is a common and life threatening disease that requires interdisciplinary treatment and often necessitates complex facial reconstruction. Standard care includes tumor resection, while reconstruction is routinely performed with free radial forearm flaps. As esthetic results are crucial for quality of life, flap size, flap vol...

Blutkonzentrate, insbesondere das plättchenreiche Fibrin, („platelet-rich fibrin“, PRF), gewinnen zunehmend an Bedeutung in der regenerativen Medizin und Zahnmedizin. Mittlerweile sind unterschiedliche Blutkonzentratsysteme für die klinische Anwendung verfügbar. Deshalb besteht Bedarf an einem vertieften Verständnis dieser Systeme und der Möglichke...

Several different biomaterials are being introduced for clinical application. However, no systematic studies could define a parameter to evaluate these materials from a clinical point of view to assess material-specific application fields. The aim of the present study was to provide clinicians with a tool to assess the regeneration pattern of bioma...

Reconstruction surgery after trauma has always been a big challenge. The use of platelet-rich fibrin (PRF) as an autologous source could help accelerate the regeneration time of bone and soft tissues. PRF is a blood concentrate system obtained through a one-step centrifugation. The 3D matrix of the PRF clot serves as a reservoir of growth factors....

Background: Guided implant surgery is considered as a safe and minimally invasive flapless procedure. However, flapless guided surgery, implant placement in post-extraction sockets and immediate loading of complete-arch fixed reconstructions without artificial gum are still not throughly evaluated. The aim of the present retrospective clinical stu...

Das Plattenepithelkarzinom stellt das sechsthäufigste Malignom des Menschen weltweit dar. Aufgrund der anatomischen Lokalisation im sichtbaren Bereich und seiner engen Lagebeziehung zu wichtigen funktionellen und ästhetischen Strukturen hat dieser bösartige Tumor erhebliche Folgen auf die Lebensqualität der Betroffenen. Daher ist eine hohe Präzisio...

Objectives To histomorphometrically evaluate the influence of autoclavation on the efficacy of extracted tooth roots (TR) used for vertical alveolar ridge augmentation. Materials and methods Upper premolars were randomly assigned to either autoclavation (TR-A) or an untreated control group (TR-C) and used as block grafts for vertical alveolar ridg...

Investigations of endogenous blood‑derived growth factors have increased in the past two decades. The initial protocols for blood concentrates, such as platelet‑rich‑plasma, utilized anticoagulants followed by bovine thrombin to catalyze fibrin polymerization. Subsequently, platelet‑rich‑fibrin (PRF) protocol was developed to eliminate anticoagulan...

Platelet-rich fibrin (PRF) is a blood concentrate derived from venous blood that is processed without anticoagulants by a one-step centrifugation process. This three-dimensional scaffold contains inflammatory cells and plasma proteins entrapped in a fibrin matrix. Liquid-PRF was developed based on the previously described low-speed centrifuge conce...