Shahlaa M. Salih

Al-Nahrain University · Biotechnology

Background: Acute myeloid leukemia (AML) is a hematopoietic disorder in which there are too many immature blood-forming cells accumulating in the bone marrow and interfering with the production of normal blood cells. It has long been recognized that AML is a clinically heterogeneous disease characterized by a multitude of chromosomal abnormalities...

Listeria monocytogenes is a pathogen that causes infectious diseases in animals. It is one of the causal agents causing abortion in infected cows. This study was conducted to detect listeria monocytogenes in aborted cows. Also to estimate the role of the milk in the distribution of the pathogen by detecting the bacteria in milk of aborted cows as w...

Non-segmental vitiligo is a developed depigmentation causing acquired, depigmented, and progressive lesions of the areas in the skin. The present study was designed to shed light on genetic and hormonal factors that may play a critical role in the pigmentary process of the non-segmented vitiligo (NSV) patients. In the first part, the levels of mela...

Cholera is endemic in over 50 countries with an estimated mortality of 100,000-120,000. Vaccination is considered the complementary key to prevent and control cholera; therefore, alternative vaccine preparations are needed. Toxin Co-regulated Pilus is part of the toxR virulence regulon, which is necessary for colonization in the intestinal mucosa....

Cholera it represent very antique disease that still take thousands of lives until this century especially in poor countries, this disease caused by Vibrio cholerae, in this study we studied the immunization activity of recombinant virulence factor (toxin co-regulated pilus) where the tcp A gene from local strain cloned and expressed in Saccharomyc...

The aim of this work was to study the association between three single nucleotide polymorphisms (SNP), rs 2227485 in IL-22 gene, rs 153109 and rs 17855750 in IL-27 gene and its levels in bladder cancer (BC) patients. One hundred bladder cancer patients and 100 healthy volunteers were registered in this study. Results showed a significant increase i...

This study was designed to identify the presence of cytochrome oxidase p450 (CYP3A4), in the hepatic cell lines which was generated from the in vitro differentiation of mouse bone marrow mesenchymal stem cells. Bone marrow meenchymal stem cells were first isolated by collecting both the femur and tibia of the mouse thigh bone, then exess tissue wer...

This study was designed to generate hepatocyte cells from mesenchymal stem cells (MSCs) isolated from mouse bone marrow via using culturing protocols for this goal. Cells of MSCs were isolated by collecting the thigh bone of 50 albino mice, both femur and tibia were collected and cells were flushed from bones and MSCs have been isolated based on th...

Bladder cancer is an abnormal cellular multiplication without control arising from the urinary bladder urothelium. Cells cycle is a strictly controlled process regulated by protein complexes composed of cyclins and cyclin-dependent kinases and also by several tumor suppressor gene protein products acting at the Go/G1 checkpoint of the cell-cycle. T...

Skin degenerative diseases, which lower the quality of life has become a colossal challenge across the globe. Despite significant number of such diseases including vitiligo and psoriasis, there has been no great progress in finding a cure to these diseases. Mesenchymal Stem Cells (MSCs) seem promising in this front as MSCs possess unique immunomodu...

The differentiation of mesenchymal stem cells (MSC) into acetylcholine secreted motor neuron-like cells, followed by elongation of the cell axon, is a promising treatment for spinal cord injury and motor neuron cell dysfunction in mammals. Differentiation is induced through a pre-induction step using Beta- mercaptoethanol (BME) followed by four day...

This study was conducted to evaluate antitumor effects of roesmarinus (Rosmarinus officinalis) extracts (aqueous and methanol) on Rhabdomyosarcoma; RD cell line and a normal cell line; mouse embryo fibroblast; MEF). Chemical detections of Rosemary extracts revealed that the aqueous and methanol extracts were positive for flavonoids, alkaloids, phen...

This study was carried out to evaluate the antitumor effect of live attenuated measles virus Schwarz (MV) vaccine strain on tumor cell lines in vitro. Live attenuated measles virus Schwarz vaccine strain was obtained from Aventis Pasteur, France. Live attenuated measles virus Schwarz strain was propagated on Vero, human Rhabdomyosarcoma (RD) and hu...

This study was carried out to assess the protective immune effect of mixed culture of Lactobacillus acidophilus and Lactobacillus gasseri in mice infected with Salmonella typhimurium. Parameters of evaluation were total and absolute count of leukocyte and phagocytosis. Fifteen albino mice divided into five groups and designated as follows: CG used...

This study was designed to evaluate the ability of Saccharomyce buolardi as effective probiotic against Shiegella flexneri. Mice treated with S. boulardii and infected with Sh. flexneri, then serum levels of Aspartate aminotransferase (AST) and Alanine aminotransferase (ALT) of treated mice were measured and histological sections were made from liv...

Glioblastoma multiforme is the most aggressive malignant primary brain tumor in humans, with poor prognosis. A new glioblastoma cell line (ANGM5) was established from a cerebral glioblastoma multiforme in a 72-year-old Iraqi man who underwent surgery for an intracranial tumor. This study was carried out to evaluate the antitumor effect of live atte...

This study was designed to evaluate the ability of Saccharomyce buolardi as effective probiotic against Shiegella flexneri. Mice treated with S. boulardii and infected with Sh. flexneri, then serum levels of Aspartate aminotransferase (AST) and Alanine aminotransferase (ALT) of treated mice were measured and histological sections were made from liv...

Thesis (M.A. in Engl.)--Cleveland State University, 1994. This thesis has been approved by the Dept. of English.