Shahina Tabassum

• Professor at Bangabandhu Sheikh Mujib Medical University

No vaccine has been more effective in reducing disease burden, especially in preventing child deaths, than measles-containing vaccine. The return on investment makes measles-containing vaccine one of the most cost-effective public health measures available. Exhaustive reviews of biological, technical, economic and programmatic evidence have conclud...

The South-East Asia Region (SEAR) adopted the goal of “measles and rubella elimination by 2023”. The goal was revised in 2019 to ‘measles and rubella elimination by 2023’ The strategies adopted to reach the goal included achieving ≥95% coverage with 2 doses of measles- and rubella-containing vaccine (MCV2; RCV2); establishing effective case-based s...

Background Hepatitis B virus DNA (HBV-DNA) assessment is recommended for diagnosing and monitoring chronic hepatitis B (CHB) patients. Quantitative hepatitis B surface antigen (qHBsAg) estimation adjunct to HBV-DNA is vital for assessing HBV chronicity and therapeutic prognosis. This study aimed to estimate the qHBsAg and compare its diagnostic per...

Background Hepatitis B virus DNA (HBV-DNA) assessment is recommended for diagnosing and monitoring chronic hepatitis B (CHB) patients. Quantitative hepatitis B surface antigen (qHBsAg) estimation adjunct to HBV-DNA is vital for assessing HBV chronicity and therapeutic prognosis. This study aimed to estimate the qHBsAg and compare its diagnostic pe...

Background The intrinsic apoptotic pathway of neutrophils in Human Immunodeficiency Virus (HIV) infection results in spontaneous neutrophil death. There is a scarcity of data regarding the gene expression of an intrinsic apoptotic pathway of neutrophils in HIV patients. Objective The objective of this study was to observe the differential expressi...

Dengue viruses are mainly transmitted by the Aedes aegypti mosquito which grows in breeding containers maintained by rain or human activity. The Reverse Transcription Loop-Mediated Isothermal Amplification (RT-LAMP) reaction was found more sensitive and can detect 10-copies of RNA template whereas; real-time PCR (qPCR) had detection limits of 100 c...

For monitoring viral load (VL) or Early Infant Diagnosis (EID) of HIV-1, real-time Polymerase Chain Reaction (qPCR) is used to perform on plasma or Dried Blood Spot (DBS) sample. The qPCR method is expensive and requires sophisticated equipment. Therefore, there is a requirement for newer and cheaper technology for VL measurement or EID. In this an...

Background: CD4 T-lymphocyte apoptosis in Human Immunodeficiency Virus infection may occur due to activation of the intrinsic apoptotic pathway or triggered via death receptors. In this study, the differential expression of the genes involved in the intrinsic apoptotic pathway was investigated on circulating CD4 T-lymphocytes of HIV-infected patien...

At the beginning of the coronavirus disease 2019 (COVID-19) pandemic in Bangladesh, there was a scarcity of ideal biocontainment facilities to detect the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), a risk group of 3 organisms. Molecular detection of SARS-CoV-2 must be performed in a BSL-2 laboratory with BSL-3-equivalent infection...

Introduction/Background* Cervical cancer is the fourth most common cancer, worldwide. Persistent infection with high-risk (HR) human papillomavirus (HPV) types is necessary for cervical cancer development. However, little is known about the influence of multiple HPV infections on cervical lesion risk. The aim of this study was to see the prevalence...

Background Hepatitis E virus (HEV) infection is endemic in Bangladesh and there are occasional outbreaks. The molecular characteristics and pathogenesis of endemic and outbreak HEV strains are poorly understood. We compared the genetic relatedness and virulence associated mutations of endemic HEV strains with outbreak strains. Methods We analyzed...

The health care systems in resource limited countries are facing major challenges in dealing with Coronavirus disease (COVID-19). In Bangladesh, a steady increase in the number of COVID-19 cases since its first report on March 8, 2020, has led to an increased demand for COVID-19 detection facilities throughout the country. The detection of severe a...

How to cite this article: Rashed Ul Islam SM, Shahera U, Jahan M, et al. Prevalent HBeAg-negative HBV DNA-positive Chronic Hepatitis B Individuals in Bangladesh. Euroasian J Hepato-Gastroenterol 2021;11(1):49-50.

Rubella virus infection during pregnancy is an important cause of blindness, deafness, congenital heart disease, and mental retardation of the foetus. Multiple sero-surveys of rubella antibody among reproductive aged females of Bangladesh showed that 20-30% of them remain susceptible to rubella as rubella vaccination is yet not included in our nati...

Hepatitis B virus (HBV) is a noncytopathic virus and billions of HBV-infected patients live uneventful lives and do not suffer from notable liver damage. However, HBV also causes progressive liver diseases characterized by hepatic inflammation, hepatic fibrosis, and liver cancer in millions of HBV-infected patients. The goal of this study was to ev...

Introduction: Hepatitis C virus (HCV) is a major cause of chronic liver disease affecting close to 170 million people worldwide. All-oral combination therapy is desirable for patients with chronic hepatitis C virus (HCV) infection. The combination of sofosbuvir (a nucleotide polymerase inhibitor) and daclatasvir, a NS5A replication complex inhibito...

Background: Although several drugs are available for treatment of chronic hepatitis B (CHB), the outcome is still far from being satisfactory. The study was conducted to develop a therapeutic strategy for CHB by a combination therapy with reduced dose and duration of antiviral drugs. Objectives: Therapeutic effects of reduced dose and shorter durat...

There are compelling epidemiological, economic, and ethical arguments for setting a global measles eradication goal. The 6 chairpersons of Regional Verification Commissions for Measles and Rubella elimination advocate that the time for courageously accelerating efforts to ensure a world where no child dies of measles, is NOW!

Dengue serotype, NS1 antigen, Real time PCR, Bangladesh

Background: Interferon-gamma induced protein 10 (IP-10), a chemokine is suggested to be involved in liver injury during Hepatitis B virus infection (HBV). The increase of IP-10 is a critical step for recruitment of inflammatory cells to the local focus of the liver and hepatopathology. This study was designed to assess the correlation of IP-10 gene...

Background & Objectives: Adult female Aedes aegypti mosquitoes require blood for development of their eggs to replenish their offspring. During blood feeding, ingestion of pathogens can occur which transmits the pathogens to the host during subsequent blood feeding. To study transmission and pathogen growth in mosquitoes, different feeding systems...

The study reports the response of first-line antiretroviral therapy (ART) by assessing CD4 and CD8 T-lymphocyte and viral load (VL) among Bangladeshi people living with HIV (PLHIV). This observational approach was conducted on 100 PLHIVs, grouped into therapy naive (n = 33), therapy initiators with CD4 T-cell count of <350 cells/µL (n = 33), and th...

Background: Cervical cancer is one of the most common types of cancer affecting women worldwide. Persistent HPV infection plays a major role in cervical cancer. The risk of cervical cancer has increased in parallel with the incidence of certain genotypes of human papilloma virus (HPV). Methods: The study was aimed to detect the distribution of HPV...

Background: Foxp3 Treg can inhibit activation, proliferation and effector functions of several kinds of other immune cells, including CD4+ and CD8+ T cells, natural killer (NK) and NKT cells and dendritic cells (DC). As such, Foxp3 Treg cells are widely considered to be the principal mediator of dominant self-tolerance immune homeostasis. In HBV...

The direct cytopathic effects of the hepatitis B virus (HBV) on subsequent liver damage are not fully understood in HBV-infected patients. However, associations between the prevalence of various HBV genotypes and the extent of liver damage have been reported from different parts of the world. The purpose of this study was to determine the distribut...

p> Background : Hepatitis B virus (HBV) infection is one of the major global health problems and this virus has many variants that differ from epidemiological distributions, transmission sources, clinical outcomes, diagnostic assays as well as therapeutic responses. HBV was also divided into 9 different subtypes which has distinct geographical dist...

The burden of chronic hepatitis B virus (HBV) infections is increasingly detected nowadays. Herein, we report a complete genome of HBV subgenotype C2 (HBV/C2) from a HBV infected patient. Complete genome analysis revealed that the isolated strain was a non-recombinant wild type and had several regular substitutions in the reverse transcriptase doma...

The burden of chronic hepatitis B virus (HBV) infections is increasingly detected nowadays. Herein, we report a complete genome of HBV subgenotype C2 (HBV/C2) from a patient with chronic HBV infection. Complete genome analysis revealed that the isolated strain was a non-recombinant wild type and had several regular substitutions in the reverse tran...

p> Background: Elucidating differences in HLA DRB1* genes distribution may be useful in understanding the molecular pathogenesis of viral hepatitis B. Objective: The aim of the study was to find out the HLA DRB1*15 gene susceptibility among acute and chronic Hepatitis B infected Bangladeshi patients. Methodology: This cross-sectional study was ca...

The number of chronic cases of hepatitis B virus (HBV) is increasing rapidly in the world. Herein, we report a complete genome of HBV subgenotype C2 (HBV/C2) with current common amino acid substitutions from a patient with chronic HBV without liver complications. Complete genome analysis revealed that the isolated strain was a non-recombinant wild...

p>Human leukocyte antigen (HLA) are cell surface glycoproteins encoded by Major Histocompatibility Complex (MHC) geneof human genome. HLA antigen frequency and haplotype distribution are useful for determining disease associations, origin, migration and genetic relationships between populations and predicting the outcome of transplantation. Thus, t...

Transmission of Hepatitis B Virus (HBV) usually occurs due to the transfusion of blood or blood products from chronic HBV (CHB) or occult HBV infected (OBI) patients. Besides serological tests e.g. HBsAg and anti‐HBc (total), detection of HBV‐DNA is necessary for the diagnosis of OBI patients. Different nucleic acid tests (NATs) including real‐time...

span>BK virus (BKV) infection has become an important concern for renal transplant recipients, as it may cause nephropathy in transplant patients receiving immunosuppressive therapy resulting in renal dysfunction and possibly, graft loss. This crosssectional study was carried out at the Department of Virology, Bangabandhu Sheikh Mujib Medical Unive...

Background: MicroRNA 122 (miR-122) is the most abundant miRNA in human liver and is released into blood in huge amount from injured liver tissues. The amount of miRNA may be associated with persistent chronic infection and disease progression. The study was designed to determine the plasma levels of microRNA-122 among chronic hepatitis B (CHB) pati...

The natural history and treatment outcome of hepatitis B viruses (HBV) infection is largely dependent on genotype, subgenotype, and the presence or absence of virulence associated mutations. We have studied the prevalence of genotype and subgenotype as well as virulence and drug resistance associated mutations and prevalence of recombinant among HB...

Recombination analysis of the 12 HBV isolates from the study using RDP4 v 4.55 program. HBV isolates were considered recombinant if detected by 5 out of 6 program (RDP, BootScan, Max Chi, Chimaera, SisScan and Topol). Recombinant isolate, recombination group major and minor parents and identity, recombinant break points, size of the recombinant fra...

The HBV reference genome sequence and sequences isolated from Bangladesh in this study used for phylogentic analysis. Genotype, subgenotype, GenBank accession number and country of origin of the reference sequences used for phylogenetic analysis. BD_HBV followed by isolate number, GenBank accession number and subgenotype of the HBV isolates from th...

Potential NAr mutations including reported NAr mutations and novel NAr mutations identified in the present study. Potential NAr mutations including reported NAr mutations and novel NAr mutations identified in the present study among different genotypes are presented. (DOCX)

Distribution of all NAR mutations identified in RT region. Schematic presentation of six HVB RT domain functional regions and (F, A, B, C, D, and E) five regions (F-A, A-B, B-C, C-D, and D-E) connecting the functional regions. Functional regions are presented as box and regions between functional regions as lines. The start and end amino acid posit...

Rubella virus infection during pregnancy is an important cause of blindness, deafness, congenital heart disease, and mental retardation of the foetus. Multiple sero-surveys of rubella antibody among reproductive aged females of Bangladesh showed that 20-30% of them remain susceptible to rubella as rubella vaccination is yet not included in our nati...

Acute lower respiratory tract infection is a common disease among the under five children. The purpose of the present study was to see the frequency and distribution of viruses causing acute lower respiratory tract infection among under-five children. This cross-sectional study was conducted in the Department of Virology at Bangabandhu Sheikh Mujib...

p>Measles is a highly contagious vaccine preventable viral disease which mainly affects children. Infection with wild measles virus induces an immune response that provides life long protection. Measles has been targeted for global eradication. In Bangladesh, there is insufficient data about the antibody responses in children following measles vacc...

p>Hepatitis E virus (HEV) infection is responsible for more than half of acute viral hepatitis (AHV) with high feto-maternal complications. Bangladesh has high prevalence of HEV infection among pregnant women. This study was undertaken to observe fetal -maternal outcome of HEV infection in Bangladeshi pregnant women . For this purpose a total of 56...

Dengue viral infections are diagnosed by detecting dengue NS1 antigen and dengue specific IgM/IgG antibody by traditional serological tests using patient's blood. Recently saliva is being used employed for diagnosis of different infectious diseases including dengue. In the present study, dengue NS1 antigen and anti-dengue IgM/IgG antibody were dete...

Hepatitis C virus (HCV) causes chronic infections that mainly affect the liver leading to hepatic fibrosis followed by development of cirrhosis and hepatocellular carcinoma (HCC). Several factors including HCV genotype have been proposed to associate with the clinical outcome of HCV infection.This study was aimed to determine whetherthere is any si...

Interferon-gamma induced protein 10 (IP-10), a chemokine is suggested to be involved in liver injury during Hepatitis B virus infection (HBV). The increase of IP-10 is a critical step for recruitment of inflammatory cells to the local focus of the liver and hepatopathology. This study was designed to assess the correlation of IP-10 gene expression...

In low prevalent countries of Human Immunodeficiency Virus (HIV) like Bangladesh, it is recommended that all HIV- positive sera tested by Enzyme Linked Immunosorbent Assay (ELISA) and/or rapid tests should be confirmed by Line Immune Assay (LIA) or Western Blot (WB) method. As these two tests are quite expensive, Indirect Immunofluorescence Assay (...

Aim Elucidating differences in gene expression may be useful in understanding the molecular pathogenesis and for developing specific markers for the outcome of hepatitis B virus (HBV) infection. In the present study, expressions of host gene interferon gamma-inducible protein (IP-10), p53, and Foxp3 were studied in hepatocytes of patients with chro...

AvailableBangladesh J Med Microbiol 2007; 01 (02): 65-68

p>The present study was done to determine the seroprevalence of the infection by Hepatitis E virus (HEV) among patients attending Bangabandhu Sheikh Mujib Medical University (BSMMU), Shahbag, Dhaka. Serum samples from 408 subjects, aged between 0-69 years, attending BSMMU from different rural and urban areas of Bangladesh were tested for HEV IgG by...

Objectives: To avoid further transmission of hepatitis B virus (HBV) infection, blood is tested for hepatitis B surface antigen (HBsAg) before transfusion. However, post-transfusion hepatitis B has been detected in clinics after transfusion of HBsAg-negative blood. The study presented here was undertaken to assess if HBsAg-negative blood is free f...

One of the major successes in the area of vaccinology is the emergence, development and usage of hepatitis B vaccine (a prophylactic vaccine against HBV). Hepatitis B vaccine has protected millions of individuals from acquiring HBV infection and has prevented liver cancer in the majority of vaccinated subjects. Although initially designed as prophy...

Background Serum-based rapid HIV testing algorithm in Bangladesh constitutes operational challenge to scaleup HIV testing and counselling (HTC) in the country. This study explored the operational feasibility of using whole blood as alternative to serum for rapid HIV testing in Bangladesh. Methods Whole blood specimens were collected from two study...

Aim: Blood is checked for hepatitis B surface antigen (HBsAg) before transfusion at Bangladesh and most other developing countries of the world, because evidences have not provided from these countries to prove that HBsAg-negative blood may also harbor hepatitis B virus (HBV) DNA. In this study, HBV-infectivity was checked in HBsAg-negative blood....

Aim: Hepatitis B virus (HBV) shows variability due to the kinetics of viral production, error prone reverse transcriptase, long term antiviral therapy and vaccination, leading to the emergence of many genotypes, subtypes and HBV variants. The present study aimed to determine HBV genotypes, subtypes and mutations in the polymerase and surface genes...

Elucidating differences in gene expression may be useful in understanding the molecular pathogenesis and for developing specific markers for the outcome of Hepatitis B virus infection. IP-10, p53 and Foxp3 were studied in liver cells to investigate a possible link between selected host gene expression and outcome of viral hepatitis B infection. The...

Aim: Hepatitis B virus (HBV) shows variability due to the kinetics of viral production, error prone reverse transcriptase, long term antiviral therapy and vaccination, leading to the emergence of many genotypes, subtypes and HBV variants. The present study aimed to determine HBV genotypes, subtypes and mutations in the polymerase and surface genes...

Elucidating differences in gene expression may be useful in understanding the molecular pathogenesis and for developing specific markers for the outcome of Hepatitis B virus infection. IP-10, p53 and Foxp3 were studied in liver cells to investigate a possible link between selected host gene expression and outcome of viral hepatitis B infection. The...

Aim: Blood is checked for hepatitis B surface antigen (HBsAg) before transfusion at Bangladesh and most other developing countries of the world, because evidences have not provided from these countries to prove that HBsAg-negative blood may also harbor hepatitis B virus (HBV) DNA. In this study, HBV-infectivity was checked in HBsAg-negative blood....

Introduction: Human papillomavirus (HPV) is a DNA virus which has tropism for epithelial cells, is the major etiological factor for development of cervical precancerous and cancerous lesions. Nearly 100 diff erent types of HPV have been characterized and thereare a large number of other types. HPV infection is one of the most common causes of sexua...

Background Hepatitis B virus (HBV) infection has many faces. Precore and core promoter mutants resemble inactive carrier status. The identification of hepatitis B core antigen (HBcAg) in hepatocytes may have variable clinical significance. The present study was undertaken to detect HBcAg in chronic hepatitis B (CHB) patients and to assess the effic...

Introduction: Human papillomavirus (HPV) is a DNA virus which has tropism for epithelial cells, is the major etiological factor for development of cervical precancerous and cancerous lesions. Nearly 100 diff erent types of HPV have been characterized and thereare a large number of other types. HPV infection is one of the most common causes of sexua...

Hepatitis B virus (HBV) infection and its liver related complications are a substantial health concern in the Asia-Pacific region. Health interventions and implementation of vaccine programs have substantially reduced the incidence of HBV infections, however, large proportion of individual remains chronically infected and the disease status of...

Background and aim Assessment of therapeutic response is important for monitoring the prognosis and to take decision for cessation of nucleoside analogues therapy in chronic hepatitis B patients. In addition to serum alanine aminotransferase (ALT), hepatitis B virus (HBV) deoxyribonucleic acid (DNA) load and HBeAg status, identification of molecula...

Dinajpur Med Col J 2014 Jul; 7 (2):92-98]

Human papilloma virus (HPV) is the most prevalent virus involved in sexually transmitted diseases worldwide, and an important public health challenge for the prevention of cervical carcinoma. The present study compared the hybrid capture II assay with conventional methods for the diagnosis of HPV infection. A total of 68 women between 25-70 years o...

Human papilloma virus is an oncogenic DNA virus, which is identified in 99.7% case of invasive cancer. It is the most prevalent virus involved in sexually transmitted diseases worldwide and an important public health challenge for the prevention of cervical carcinoma. Cervical cancer is the second cause of malignant neoplasia and death in women. Th...

Background: Both real-time-polymerase chain reaction (PCR) and hybrid capture 2 (HC2) assay can detect and quantify hepatitis B virus (HBV) DNA. However, real-time-PCR can detect a wide range of HBV DNA, while HC2 assay could not detect lower levels of viremia. The present study was designed to detect and quantify HBV DNA by real-time-PCR and HC...

p> Backgrounds: As there is no published data regarding the response to Anti-retroviral therapy (ART) among HIV patients from Bangladesh. The present study was designed to determine the immunological and virological responses of HIV infected Bangladeshi adults starting ART. Objectives: To monitor the changes of CD4 and CD8 T-lymphocyte count and Vi...

The present study evaluates the intracellular expression of the hepatitis core antigen (HBcAg) in chronic hepatitis B (CHB) patients from Bangladesh in relation with serological, virological and histological variables. A cross sectional study was carried out among 70 patients incidentally diagnosed as HBV chronic carrier who have undergone liver bi...

Hepatitis G virus (HGV) is a RNA virus, which was identified in 1995-1996 as a transfusion transmissible virus and is associated with acute or chronic hepatitis. The sero-prevalence of hepatitis G virus was evaluated among various risk groups and healthy controls from Bangladesh. A total of 252 subjects comprising of Intra-venous drug users (n-40),...

Bangladesh has the highest level of incidence and mortality rates due to cervical cancer among women. The prevalence of cervical cancer in Bangladeshi women is 25-30/100,000. Human papillomavirus is an important cause of cervical cancer. The study was conducted to assess the immunogenicity and safety profile of human papillomavirus-16/18 AS04-adjuv...

The role of blood and blood products in acquisition of cytomegalovirus (CMV) infections following transfusion was reviewed in this study. CMV IgG prevalence was particularly high in Bangladesh. Thus 97% of the study groups were found to be CMV IgG positive. The present study showed that CMV IgM antibody prevalence was significantly higher in multip...

A large outbreak of dengue fever (DF)/dengue haemorrhagic fever (DHF) occurred in Dhaka city, Bangladesh, in 2000. The present study was conducted on 105 clinically-suspected cases of DF to confirm the diagnosis, determine the major clinical manifestations and correlate the haemorrhagic manifestations with different dengue serotypes circulating dur...

Abstract Dengue,virus isolation and,serotyping was,carried out in 105 clinically-suspected,cases of dengue fever by the mosquito,inoculation,and,cell culture techniques,to find a suitable virus isolation technique,for a resource-constrained country like Bangladesh and to identify the serotypes responsible for the dengue,outbreak in 2000. Virus was...

Of the 63 Shigella strains isolated from stool cultures from 200 patients who attended a district hospital in Bangladesh with bloody diarrhoea, 37 (59%) were S. dysenteriae type 1, 25 (39%) were S. flexneri and only one (2%) was S. sonnei. Over half (54%) of the Shigella isolates came from children aged < 10 years. Most (89%) of the isolates of S....