Shahid Mansoor

Shahid Mansoor

About

385
Publications
97,511
Reads
How we measure 'reads'
A 'read' is counted each time someone views a publication summary (such as the title, abstract, and list of authors), clicks on a figure, or views or downloads the full-text. Learn more
11,013
Citations

Publications

Publications (385)
Article
Full-text available
Cotton is an important crop that produces fiber and cottonseed oil for the textile and oil industry. However, cotton leaf curl virus disease (CLCuD) stress is limiting its yield in several Asian countries. In this study, we have sequenced Mac7 accession, a Gossypium hirsutum resistance source against several biotic stresses. By aligning with the Go...
Article
Full-text available
Bemisia tabaci species complex (whitefly) is one of the most dangerous pests that destroy many important crops worldwide. It causes damage to the host plant by feeding on phloem sap as well as transmitting a wide range of devastating plant viruses (especially begomoviruses) that cause severe epidemics on crops. To fend off the menace, modern genomi...
Preprint
CRISPR/Cas system has emerged as the most efficient genome editing technology for both eukaryotic and prokaryotic cells. Recently, biologist have started using CRISPR/Cas9 as a defence machinery in plants against DNA viruses by targeting conserve regions of their genome. Considerable resistance requires formation of stable transgenic lines with mul...
Article
Full-text available
The ensuing heat stress drastically affects wheat plant growth and development, consequently compromising its grain yield. There are many thermoregulatory processes/mechanisms mediated by ion channels, lipids, and lipid-modifying enzymes that occur in the plasma membrane and the chloroplast. With the onset of abiotic or biotic stresses, phosphoinos...
Article
Common wheat is a major source of nutrition around the globe, but unlike maize and rice hybrids, no breakthrough has been made to enhance wheat yield since Green Revolution. With the availability of reference genome sequence of wheat and advancement of allied genomics technologies, understanding of genes involved in grain yield components and disea...
Article
Potato (Solanum tuberosum L) is an important staple food around the world, and potato virus Y (PVY) is a major constraint to potato production. The VPg of PVY interacts with the initiation factor eIF4E, that works as a susceptibility factor during infection. The interaction between eIF4E protein and VPg has been disrupted by CRISPR/Cas9. A homozygo...
Article
Pedilanthus leaf curl virus (PeLCV) is a widely encountered monopartite begomovirus with a wide host range that usually associates with a betasatellite. The cloned PeLCV used was highly infectious to Nicotiana benthamiana plants by agroinoculation and symptoms were enhanced by co-inoculation with tobacco leaf curl betasatellite (TbLCB). The study i...
Article
Alternative splicing regulation (AS) is an important post-transcriptional gene regulatory mechanism for increasing the complexity and diversity of transcriptomes and subsequently proteomes. In this study, we performed transcriptomic analysis of Gossypium arboreum to explore the process of alternative splicing regulation in pollen allergen (Ole e 1)...
Article
Full-text available
Although Pakistan has rich biodiversity, many groups are poorly known, particularly insects. To address this gap, we employed DNA barcoding to survey its insect diversity. Specimens obtained through diverse collecting methods at 1,858 sites across Pakistan from 2010-2019 were examined for sequence variation in the 658 bp barcode region of the cytoc...
Article
Full-text available
Soybean is considered one of the important crops among legumes. Due to high nutritional contents in seed (proteins, sugars, oil, fatty acids, and amino acids), soybean is used globally for food, feed, and fuel. The primary consumption of soybean is vegetable oil and feed for chickens and livestock. Apart from this, soybean benefits soil fertility b...
Article
Whitefly inflicts both direct and indirect losses to cotton crop. Whitefly resistant cotton germplasm is a high priority and considered among the best possible solutions to mitigate this issue. In this study, we evaluated cotton leaf curl disease (CLCuD) resistant cotton line Mac7 against whitefly stress. Furthermore, we utilized the already availa...
Article
Modern wheat shows phenomenal evolutional success and adaptability to a range of environments owing to polyploidization; however, during its hybridization process a major genetic gain has been overlooked. Recently, Gaurav et al. emphasized harnessing genetic diversity from wheat wild progenitor Aegilops tauschii for the improvement of hexaploid whe...
Article
CRISPR-Cas9 creates remarkable possibilities to modify targeted regions in genomic DNA. However, CRISPR-Cas-mediated DNA double-stranded breaks (DSBs), that tend to generate random insertions or deletions, limit this technology. Recently, Anzalone et al. developed a 'twin prime editing' tool to replace, integrate, or delete large genomic DNA sequen...
Article
Full-text available
Globally, Whitefly ( Bemisia tabaci ) is one of the most important insect pests of crops that causes huge economical losses. The current study was designed to exclusively screen the B. tabaci species in the cotton field of Pakistan during 2017–2020 and have to conduct comparative analysis of B. tabaci species in Asia where Asia II 1 has been report...
Chapter
Infectious plant pests and pathogens are high risk threats for the provision of food security and fulfilling the increased demand of better nutritional value. They cause declines in crop yield and hence cause huge economic losses. Current detection platforms, that is, polymerase chain reaction (PCR), quantitative PCR, enzyme-linked immunosorbent as...
Chapter
Nanotechnology has the potential to boost agricultural production by enabling nanosensors with electrical, optical, mechanical, and wireless properties for improving the agricultural practices. Reducing crop yield, pathogen attack, and environmental stresses are the major challenges faced worldwide. New and advance technologies are required for eff...
Article
Gene drives for control of vector-borne diseases have been demonstrated in insects but remain challenging in plants. Theoretically, they could be transformative in speeding breeding programs and contributing to food security through providing novel weed control methods. Zhang et al. now report the possibility of implementing gene drive in plants fo...
Article
Virus-induced gene silencing (VIGS) is a reverse genetic tool used to identify the function of individual genes by reducing their expression. Here VIGS was applied to identify the role of four genes in G. hirsutum; Polyphenol Oxidase (GhPPO), Phosphatidylserine Synthase (GhPSS), Anthocyanidin synthase (GhANS), and Anthocyanidin Reductase (GhANR) in...
Article
Geminiviruses are small DNA viruses with single-stranded circular genomes that encode for few known proteins. Recently, Gong et al. reconsidered their protein identification criteria and identified open reading frames that encode small proteins with specific cellular localizations and virulence functions. Their data warrants exploration of such sma...
Article
Transformation of the chloroplast genome offers key advantages over traditional methods for generating transgenic plants, but this approach is limited to a few plant species. Nakazato et al. have developed a novel technique that will help to extend the technology to other plant species that are recalcitrant to current tissue culture-based chloropla...
Preprint
Full-text available
Clustered regularly interspaced palindromic repeats (CRISPR) and associated Cas9 nuclease (CRISPR-Cas9) systems provide adaptive immunity to prokaryotes against infectious phage particles that can be engineered as a genome-editing tool. Guided by an RNA strand, the class II type II CRISPR-Cas9 system can be employed to provide resistance against pl...
Article
Full-text available
Cotton leaf curl disease (CLCuD) is one of the major limiting factors affecting cotton production in Pakistan for the last three decades. The disease is caused by begomoviruses of the family Geminiviridae. RNA interference (RNAi) is a promising tool that has been proved effective against several pathogens. Using RNAi, different genomic regions of g...
Article
Full-text available
Cotton production is seriously affected by the prevalent cotton leaf curl disease (CLCuD) that originated from Nigeria (Africa) to various parts of Asia including Pakistan, India, China and Philippines. Due to CLCuD, Pakistan suffers heavy losses approximately 2 billion USD per annum. Numerous reports showed that CLCuD is associated with multiple s...
Article
Full-text available
The development of portable and sensitive biosensors for the label-free detection of DNA has influenced fundamental biological research as well as advanced applications. Here, we report the novel microchip-based electronic devices for the efficient and sensitive detection of a crop virus i.e. Cotton leaf curl Khokran virus-Burewala strain (CLCuKoV-...
Chapter
The agriculture sector is facing tremendous pressure to meet the demands of an increasing population and to ensure future food security. About a 60% increase in crop production is required to fulfill global demand. Pests and diseases cause 11–30% loss to the world's production. So, developing resistance against different diseases is a very importan...
Article
Full-text available
Bt cotton expressing Cry1Ac is being cultivated in Pakistan. It has been observed that pink bollworm may have developed resistance against single Bt gene (Cry1Ac). For durable resistance, insect resistant NIBGE-1601 cotton harboring double gene Cry1Ac-Cry2Ab construct was developed. There was a need to characterize NIBGE-1601 event for intellectual...
Article
The ongoing global spread of Tomato yellow leaf curl virus (TYLCV) poses an endemic threat to tomato production in all tropical and sub-tropical regions of the world. In Oman, TYLCV evolved into the new species Tomato leaf curl Oman virus (ToLCOMV) by recombination with Papaya leaf curl virus (PaLCuV). In March 2017, during a field survey of Sindh...
Article
Full-text available
Plants are regularly exposed to biotic and abiotic stresses that adversely affect agricultural production. Omics has gained momentum in the last two decades, fueled by statistical methodologies, computational capabilities, mass spectrometry, nucleic-acid sequencing, and peptide-sequencing platforms. Functional genomics—especially metabolomics, tran...
Article
Full-text available
Cotton leaf curl disease (CLCuD) is the most important limiting factor for cotton production in Pakistan. The CLCuD passed through two major epidemics in this region with distinct begomoviruses/satellites complexes. Since 2015 the disease has again started to appear in epidemic form, causing heavy losses to cotton crop, which we termed as the “thir...
Article
Two field-collected tomato plants, with severe and mild tomato leaf curl disease symptoms, were shown to be infected with tomato leaf curl New Delhi virus. The plant with severe symptoms was shown to additionally contain tobacco leaf curl betasatellite (TobLCuB). Inoculation of Nicotiana benthamiana plants with the cloned components showed the seve...
Chapter
Geminiviridae is one of the most important families in plant viruses with a single-stranded DNA genome comprises one or two genomes (DNA-A and DNA-B) of 2.5–5.2Kb. These viruses infect economically important food and fiber crops worldwide. Till to date nine genera (Mastrevirus, Curtovirus, Becurtovirus, Turncurtovirus, Begomovirus, Capulavirus, Top...
Article
Betasatellites are single-stranded DNA molecules associated with most monopartite begomoviruses of family Geminiviridae. They are symptoms modulating molecules and depend on their helper viruses for replication, encapsidation and movement. To investigate the genetic diversity of cotton leaf curl Multan betasatellite (CLCuMuB), different Pakistani i...
Article
Phyllosphere microbial communities inhabit the aerial plant parts, such as leaves and flowers, where they form complex molecular interactions with the host plant. Contrary to the relatively well-studied rhizosphere microbiome, scientists are just starting to understand, and potentially utilize, the phyllosphere microbiome. In this article, we summa...
Article
Resistance (R) genes are directly or indirectly involved in the activation of the plant immune systems. Despite their essential roles in defense against a variety of pathogens, very little is known about their classification as well as associated decoy domains, particularly in crop plants. Using advanced bioinformatics strategies, this study provid...
Article
Full-text available
Codiaeum variegatum (common name, garden croton) is an ornamental plant grown for its bright yellow variegated leaf morphology. Two C. variegatum plants with upward leaf curling and vein swelling symptoms were collected in Faisalabad, Pakistan. Sequencing of clones obtained by PCR amplification with specific primers showed one plant infected with t...
Article
Viruses of the genus Begomovirus (family Geminiviridae) are a threat to agriculture and food security throughout the warmer parts of the world. Begomoviruses are transmitted by the whitefly Bemisia tabaci and have genomes consisting of one (monopartite) or two (bipartite) circular, single-stranded (ss) DNA molecules. Most monopartite begomoviruses...
Article
Photosynthesis is an important biochemical reaction that forms the basis of all food chains. Its efficiency is considered a key determinant of crop productivity. Therefore, improving photosynthetic efficiency has been a focus of intensive research. Here, we highlight simple approaches, recently reported by Chen et al. and Degen et al., to increase...
Article
RNA silencing or RNA interference (RNAi) is an endogenous mechanism of gene regulation and adaptive defence against invading microbes of higher organisms, triggered by double-stranded RNA. Viruses circumvent this host defence by encoding specific proteins, RNA silencing suppressors (RSSs). Evidence has been mounting that, in common with RNA viruses...
Article
Whitefly (Bemicia tabaci) is an insect pest that causes severe losses in economically important crops by sucking the plant sap and transmitting plant viruses. We used the promising new approach of plant-mediated artificial miRNA (amiRNA) expression against three essential genes of whitefly. The Arabidopsis thaliana miR159 precursor was modified and...
Article
Full-text available
In 2016, begomovirus-like symptoms were observed in mungbean (Vigna radiata) and black gram (Vigna mungo) in Pakistan. Symptomatic and asymptomatic leaf samples were collected and total genomic DNA was extracted. Full length begomovirus and betasatellite genomes were PCR amplified and cloned in plasmid pTZ/57RT cloning vector. The cloned molecules...
Article
Full-text available
Rice (Oryza sativa) is an important staple food crop worldwide; to meet the growing nutritional requirements of the increasing population in the face of climate change, qualitative and quantitative traits of rice need to be improved. Stress-tolerant crop varieties must be developed with higher yields and increased adaptability to the changing clima...
Article
Tomato leaf curl New Delhi virus (ToLCNDV), a single-stranded (ss)DNAvirus(family; Geminiviridae), has become a constraint to the production of crops of the Cucurbitaceae, Solanaceae and Malvaceae across the sub-Continent, south east Asia and, more recently, the western Mediterranean basin. Transgenic Nicotiana benthamiana plants constitutively exp...
Article
Tomato leaf curl New Delhi virus (ToLCNDV), a single-stranded (ss)DNA virus(family; Geminiviridae), has become a constraint to the production of crops of the Cucurbitaceae, Solanaceae and Malvaceae across the sub-Continent, south east Asia and, more recently, the western Mediterranean basin. Transgenic Nicotiana benthamiana plants constitutively ex...
Article
Full-text available
Basmati rice is famous for its flavor, aroma and long grain around the globe. Its demand is increasing worldwide especially in Asia. However, its production is threatened by various problems faced in the field which results in major crop losses. One of the major problems is bacterial blight caused by Xanthomonas oryzae pv. oryzae (Xoo). Xoo hijacks...
Article
Full-text available
Volkameria inermis (synonym Clerodendrum inerme) is a well-known ornamental plant commonly used for hedging around lawns and gardens. In 2017 and 2019, V. inermis plants growing at two different locations in Faisalabad, Pakistan (GPS coordinates 31.39716 N, 73.02639E and 31.40973 N, 73.15507E respectively) were found to exhibit typical begomovirus...
Article
Full-text available
Improvement in nitrogen use efficiency of crop plants has been a major challenge in agricultural research. A barley alanine aminotransferase (HvAlaAT) gene was codon optimized and got synthesized to achieve optimal expression in tobacco plants. Synthetic alanine aminotransferase (co-AlaAT) gene was cloned under 2XCaMV35S promoter and transformed in...
Article
Aquaporins (AQPs) are water channel proteins that play a significant role in drought stress. Although the AQPs identified in multiple plant species, there is no detailed evolutionary and comparative study of AQPs regarding chickpea plant. The current study involved evolutionary analyses coupled with promoter and expression analyses of chickpea AQPs...
Chapter
Geminiviruses cause major damage to crop plants and evolve through mutations, recombination and pseudo-recombination, expanding their host range and becoming pandemic due to international trade and invasive vector species. Management of geminiviruses consists of breeding resistant crops by employing natural resistance genes from different cultivate...
Chapter
Cotton leaf curl disease (CLCuD) is the most devastating disorder of cotton caused by begomoviruses (family Geminiviridae) and betasatellite complex transmitted exclusively by whiteflies (Bemisia tabaci). This disease is endemic in the Indian sub-continent and sporadically occurs in Africa. Affected plants show the distinct symptoms such as vein th...
Article
Full-text available
Begomoviruses interfere with host plant machinery to evade host defense mechanism by interacting with plant proteins. In the old world, this group of viruses are usually associated with betasatellite that induces severe disease symptoms by encoding a protein, βC1, which is a pathogenicity determinant. Here, we show that βC1 encoded by Cotton leaf c...
Article
Luffa plants showing symptoms of begomovirus infection were collected from Vehari, Punjab in 2016. RCA and PCR amplifications of begomovirus genomic components showed the presence of a typical Old World bipartite begomovirus Tomato leaf curl New Delhi virus (ToLCNDV). Interestingly, Ageratum conyzoides symptomless alphasatellite and CLCuMB were als...
Article
Full-text available
Begomoviruses; have devastating impact on the yield of many economically important crops around the globe. During field survey in 2016, typical symptoms of begomovirus infections were observed in green beans in district Hazara, Pakistan. We identified a new strain of Malvastrum yellow vein Lahore virus in green beans for the first time in Pakistan.
Article
Functional genomics is at the core of studying the exact function of genes. However, homozygous knockouts of essential and pleiotropic effectors (almost 10% of the genome) are not always possible, thus, functions of these genes remain obscured. The tissue-specific genome editing tool (CRISPR-TSKO) recently described by Decaestecker et al., can char...
Article
Full-text available
Vacuolar H +-pyrophosphatase (VP1) gene is an efficient proton pump that develops a proton gradient to promote Na + /H + antiporter activity. Thus, it plays vital role in conferring salinity tolerance in plants. This study was planned to evaluate the performance of Hordium vulgare vacuolar H +-pyrophosphatase (HVP1) gene in wheat (Triticum aestivum...
Article
The whitefly (Bemicia tabaci) is an insect pest that causes severe losses in economically important crops by sucking the plant sap and transmitting plant viruses. We used the promising new approach of plant-mediated artificial miRNA (amiRNA) expression against three essential genes of whitefly.The Arabidopsis thaliana miR159 precursor was modified...
Article
Abiotic stresses, including drought, salinity, temperature, and heavy metals, pose a major challenge for crop production and cause substantial yield reduction worldwide. Breeding tolerant cultivars against these abiotic stresses is the most sustainable and eco-friendly approach to cope with this challenge. Advances in genome editing technologies pr...
Article
Full-text available
Croton bonplandianus is a common weed across Pakistan and the subcontinent. Plants showing symptoms typical of begomovirus infection, including leaf curling, vein yellowing, and vein swelling were collected and analyzed for the presence of begomoviruses and associated satellites. Cloning and sequencing of products produced by rolling circle amplifi...
Article
Full-text available
Cultivated cotton (Gossypium hirsutum) is the most important fiber crop in the world. Cotton leaf curl disease (CLCuD) is the major limiting factor and a threat to textile industry in India and Pakistan. All the local cotton cultivars exhibit moderate to no resistance against CLCuD. In this study, we evaluated an exotic cotton accession Mac7 as a r...
Article
Full-text available
Cotton is the main fiber producing crop globally, with a significant impact on the economy of Pakistan. Bt cotton expressing a Cry1Ac gene is grown over a large area in Pakistan, however, there is a major concern that bollworms may develop resistance. Here we have used a durable resistance strategy against bollworms by developing a double gene cons...
Preprint
Full-text available
Quality of milk is determined by multiple factors including microbial load, somatic cell score, content percentages and absence of adulterants. However, in term of genetics, milk quality can be linked with beta casein. Beta casein is a milk protein having genetic variants, one of which is believed to be linked with progression of many diseases incl...
Article
Full-text available
Background: Whiteflies (Bemisia tabaci) are phloem sap-sucking pests that because of their broad host range and ability to transmit viruses damage crop plants worldwide. B. tabaci are now known to be a complex of cryptic species that differ from each other in many characteristics such as mode of interaction with viruses, invasiveness, and resistan...