Sezayi Özübek- DVM, PhD
- Professor at Fırat University
Sezayi Özübek
- DVM, PhD
- Professor at Fırat University
About
85
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1,395
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Introduction
Current institution
Additional affiliations
June 2012 - present
Publications
Publications (85)
This study was carried out to investigate Anaplasma important for veterinary and public health in sheep and goats in Niğde province in Türkiye by using molecular methods.
Blood samples were taken from randomly selected 690 animals (520 sheep and 170 goats), which were between 1 and 10 years old and from different study sites in Niğde by using the v...
This research topic aims to explore the critical aspects of pathogens transmitted by ectoparasites, focusing on their epidemiology, genetic characterization, and interactions with hosts, ectoparasite microbiota, and environmental factors. We invite contributions that examine how these pathogens impact animal health and the potential for innovative...
Ovine babesiosis, caused by Babesia ovis, is a significant tick-borne disease affecting sheep globally, with severe economic implications for sheep farming, particularly in Türkiye. Babesia ovis is transmitted exclusively by adult Rhipicephalus bursa ticks, but the potential role of infected larval stages in modulating disease severity has remained...
Babesiosis, a tick-borne disease caused by various Babesia species, is primarily associated with livestock but has increasingly been reported in dogs worldwide. This research investigates the prevalence of Babesia species in shelter dogs located in Diyarbakır province, Türkiye. Blood samples from 80 asymptomatic dogs were processed for DNA extracti...
Simple Summary
In this study, pathogen-free Rhipicephalus bursa colonies were established for vector competence research, and their life cycle parameters were analyzed. Initially, engorged R. bursa females were collected from naturally infested sheep, goats, and cattle. The engorged females were placed in an incubator to lay eggs and produce larvae...
Hepatozoon canis, a protozoan parasite, is the primary cause of canine hepatozoonosis worldwide, typically causing subclinical infection in dogs but potentially leading to severe illness when accompanied by other pathogens. This study investigates the genetic diversity of H. canis in dogs and Rhipicephalus sanguineus sensu lato ticks using bioinfor...
Small ruminant babesiosis remains a neglected disease despite causing significant economic losses to sheep and goat herds in many regions around the world. The pathogenesis and clinical manifestations of ovine babesiosis are well-known, but there is a lack of information regarding caprine babesiosis. Since the discovery of the first Babesia spp. in...
Babesia ovis, commonly associated with ovine babesiosis, poses a significant threat to sheep health, often resulting in severe clinical manifestations and high mortality rates. However, the impact of B. ovis on goats has remained uncertain, prompting us to investigate its pathogenicity in caprine hosts. Experimental infections using B. ovis-infecte...
This study presents the first comprehensive analysis of the mitochondrial genome of Babesia ovis, a significant pathogen in ovine babesiosis in Türkiye. The B. ovis mitochondrial genome is a linear monomeric molecule of 6015 bp with an A + T content of 70.5%, featuring terminal inverted repeats (TIRs) at both ends. It encodes three essential protei...
Simple Summary
We developed a new test to detect Babesia aktasi, a parasite that infects goats, using a method called semi-nested PCR. This method focuses on a specific part of the parasite’s DNA to ensure accuracy. We checked the test against several other similar parasites to make sure it only detected B. aktasi, which it did successfully. To see...
Simple Summary
In this study, an experimental investigation was conducted to assess the pathogenicity of Babesia aktasi in immune-suppressed sheep. For this purpose, five immune-suppressed lambs under one year of age were infected by intravenous injection of fresh blood containing approximately 9.2% and 12% parasitemia of B. aktasi. Following paras...
Babesia ovis, transmitted by Rhipicephalus bursa ticks, is the causative agent of ovine babesiosis, a disease characterized by fever, anemia, hemoglobinuria, and high mortality in sheep. This study investigates whether sheep that survived babesiosis without treatment can serve as a source of infection for B. ovis-free host-seeking R. bursa larvae i...
Simple Summary
The tick species involved in the transmission of Babesia aktasi, which is widespread in the Mediterranean region, is unknown. However, studies have shown that Rhipicephalus bursa is the most common tick species in the regions where the prevalence of B. aktasi is widespread. This finding increases the possibility that R. bursa may ser...
Babesiosis, caused by Babesia ovis, is a major seasonal issue in sheep, particularly in countries like Türkiye with high Rhipicephalus bursa tick populations. Previous studies employing various methods such as microscopy, serology, or molecular techniques have reported different epidemiological data concerning ovine babesiosis. Addressing this know...
Babesiosis is an acute and persistent tick-borne disease caused by protozoan parasites of the genus Babesia. These hemoparasites affect vertebrates globally, resulting in symptoms such as high fever, anemia, jaundice, and even death. Advancements in molecular parasitology revealed new Babesia species/genotypes affecting sheep and goats, including B...
Ovine babesiosis caused by Babesia ovis is an economically significant disease. Recently, a few B. ovis-specific proteins, including recombinant B. ovis secreted antigen-1 (rBoSA1), have been identified. Immunological analyses revealed that rBoSA1 resides within the cytoplasm of infected erythrocytes and exhibits robust antigenic properties for det...
Anaplasma ovis is a tick-borne obligated intraerythrocytic bacterium that infects domestic sheep, goats, and wild ruminants. Recently, several studies have been carried out using 16S rRNA and msp4 genes to identify the genetic diversity of A. ovis. Instead of these genes, which are known to be highly stable among heterologous strains, Msp1a, which...
Small ruminant piroplasmosis is the hemoparasitic infection of sheep and goats caused by Babesia and Theileria species responsible for clinical infections with high mortality outcomes. The disease is transmitted by ixodid ticks and prevalent in the tropical and subtropical regions of the world, including Türkiye. A prevalence survey, using molecula...
A novel Babesia sp. infecting goats was discovered based on the molecular findings obtained in the current study, which was conducted in the Mediterranean region of Türkiye. The goal of this study was to isolate this species of Babesia (Babesia sp.) infecting goats in vivo and to assess the genetic and morphological characterization of the parasite...
The apicomplexan tickborne parasites Babesia bovis and B. bigemina are the major causative agents of bovine babesiosis, a disease that negatively affects the cattle industry and food safety around the world. The absence of correlates of protection represents one major impediment for the development of effective and sustainable vaccines against bovi...
Citation: Asif, M.; Ben Said, M.; Vinueza, R.L.; Leon, R.; Ahmad, N.; Parveen, A.; Khan, A.; Ejaz, A.; Ali, M.; Khan, A.U.; et al. Seasonal Investigation of Anaplasma marginale Infection in Pakistani Cattle Reveals Hematological and Biochemical Changes, Multiple Associated Risk Factors and msp5 Gene Conservation.
The ikeda and chitose genotypes of Theileria orientalis, which for many years were thought to be benign, cause a disease that results in significant economic losses in the cattle industry. This study was carried out in order to determine the genotypes of T. orientalis in cattle in Kyrgyzstan, and 149 archived DNA samples known to be T. orientalis w...
Theileriosis is one of the most frequently reported tick borne diseases in tropical and subtropical regions and leads to annual economic losses, such as the reduced dairy products and increased casualties. Tropical theileriosis is caused by Theileria annulata and the present study was designed to improve our knowledge of Theileria annulata infectio...
Citation: Al-Hamidhi, S.; Parveen, A.; Iqbal, F.; Asif, M.; Akhtar, N.; Elshafie, E.I.; Beja-Pereira, A.; Babiker, H.A. Diversity and Genetic Structure of Theileria annulata in Pakistan and Other Endemic Sites. Pathogens 2022, 11, 334. https://doi.
Bovine babesiosis, caused by Babesia bovis, is an economically significant tick-borne disease that imposes restrictions to livestock production worldwide. Current methods to control bovine babesiosis have severe limitations and novel approaches, including transmission-blocking vaccines, are needed. Members of the widely conserved CCp family are mul...
Purpose
In Pakistan, a major constrain to goat farming is the tick and tick-borne diseases that results in financial losses to livestock farmers. This study was conducted to report the molecular prevalence of Anaplasma (A.) marginale in goat blood samples collected during four seasons from Khanewal district in Punjab (Pakistan).
Methods and result...
Raising small ruminants is the main source of income for farmers in Pakistan. Economic losses caused by Toxoplasma gondii to small ruminants have been reported worldwide, however reports on molecular detection of T. gondii are lacking in Pakistan despite a large goat population. The current study was carried out from March 2019 till February 2020 t...
Raising small ruminants is the main source of income for farmers in Pakistan. Economic losses caused by Toxoplasma gondii to small ruminants have been reported worldwide, however reports on molecular detection of T. gondii are lacking in Pakistan despite a large goat population. The current study was carried out from March 2019 till February 2020 t...
Purpose In Pakistan, a major constrain to goat farming is the tick and tick-borne diseases that results in financial losses to livestock farmers. This study was conducted to report the molecular prevalence of Anaplasma (A.) marginale in goat blood samples collected during four seasons from Khanewal district in Punjab (Pakistan). Methods and results...
The present study was designed to report the molecular prevalence of T. annulata in cattle blood samples collected from Punjab in Pakistan. A total of 428 cattle blood samples were collected from Districts Lodhran (n = 218) and Dera Ghazi Khan (n = 210). The prevalence of T. annulata was determined by the amplification of a fragment from its cytoch...
Anaplasmosis, caused by intracellular gram-negative bacteria Anaplasma marginale is one of the most frequently reported tick-borne disease (TBDs) in tropical and sub-tropical countries, including Pakistan. In the present study, a total of 428 cattle blood samples were collected to examine the prevalence and phylogenetic origin of A. marginale in tw...
Equine Piroplasmosis (EP) is an infectious disease caused by the hemoprotozoan parasites Theileria equi, Babesia caballi, and the recently identified species T. haneyi. Hereby, we used a multiplex PCR (mPCR) targeting the 18S rRNA gene of T. equi and B. caballi for the simultaneous detection of EP in Egyptian equids and examined the presence of T....
Theileria ovis and Anaplasma marginale are intracellular pathogens affecting a wide range of animals, causing huge economic losses worldwide. The present study reports the molecular evidence of Theileria ovis and Anaplasma marginale in sheep blood samples (N = 218) collected from Layyah district in Punjab (Pakistan), where economy heavily relies on...
Theileria ovis and Anaplasma marginale are intracellular pathogens affecting a wide range of animals, causing huge economic losses worldwide. The present study reports the molecular evidence of Theileria ovis and Anaplasma marginale in sheep blood samples (N = 218) collected from Layyah district in Punjab (Pakistan), where economy heavily relies on...
Anaplasma centrale (A. centrale) is an obligate red blood cell residing tick transmitted rickettsiae that has not been studied extensively for its prevalence in cattle along with its epidemiology. Aim of this investigation was to report the seasonal prevalence, phylogeny and epidemiological parameters associated with the prevalence of A. centrale i...
Anaplasma centrale (A. centrale) is an obligate red blood cell residing tick transmitted rickettsiae that has not been studied extensively for its prevalence in cattle along with its epidemiology. Aim of this investigation was to report the seasonal prevalence, phylogeny and epidemiological parameters associated with the prevalence of A. centrale i...
Simple Summary
We explored the existence of Anaplasma phagocytophilum and related variant in samples of goats and sheep obtained from Antalya and Mersin provinces, representative of Mediterranean region of Turkey. Based on 16S rRNA and groEL genes of A. phagocytophilum and related variants, we examined blood samples by polymerase chain reaction (PC...
Theileria annulata is the cause of tropical theileriosis in cattle in Pakistan, where it has a significant impact on the cattle industry. Here we report the molecular detection and seasonal prevalence and blood parameters of T. annulata infection in crossbred, Holstein Frisian and Sahiwal breed in Layyah District in the Punjab. In total, 844 blood...
Bovine babesiosis is a global tick-borne disease that causes important cattle losses and has potential zoonotic implications. The impact of bovine babesiosis in Turkey remains poorly characterized, but several Babesia spp., including B. bovis, B. bigemina, and B. divergens, among others and competent tick vectors, except Rhipicephalus microplus, ha...
Anaplasma marginale a tick-borne rickettsial pathogen causes high morbidity and mortality among cattle in tropical and subtropical regions. Major surface protein 1 alpha (Msp1α) is a stable genetic marker for identifying A. marginale. Our aims were to investigate the genetic diversity of A. marginale based on tandem repeats and microsatellites of t...
Back ground: Anaplasmosis is known as yellow bag or yellow fever and it is a tick borne disease caused by obligate intercellular gram negative bacteria, Anaplasma marginale.
Aim: Present study is reporting seasonal prevalence, epidemiology and phylogeny of Anaplasma marginale in three cattle breeds from District Layyah, Southern Punjab, Pakistan. M...
Aim
The present study was designed to check the molecular detection of Anaplasma marginale and Theileria annulata in blood samples of horses and donkeys collected from Dera Ghazi Khan District in Punjab and to document their phylogenetic origin and their association with studied epidemiological factors (sex and age) and complete blood count paramet...
Bovine piroplasmosis is a tick-borne disease caused by apicomplexan hemoparasites of the genera Theileria and Babesia. This study was carried out to assess the presence and frequency of piroplasm parasites in apparently healthy cattle in Kyrgyzstan. A total of 454 blood samples were collected from animals of various ages in eight villages located i...
Tick-borne pathogens cause serious health problems and loss of productivity in domesticated and wild animals. A molecular study was performed to detect the frequency of infection with Anaplasma/Ehrlichia (A/E) in cattle from Karaman province of Turkey. Venous blood samples were taken from 150 apparently healthy cattle in 2016. After amplification t...
Theileria orientalis is usually a benign parasite but some genotypes cause infection and economic losses to the cattle industry. This study was carried out to determine T. orientalis genotypes in cattle. T. orientalis positive 63 sample were analyzed by amplifying the MPSP gene region by PCR. As a result of the SSCP analysis, samples with different...
Canine monocytic ehrlichiosis, an important tick-borne disease caused by Ehrlichia canis, is cosmopolitan but particularly prevalent in tropical and subtropical regions. In Turkey, the genetic diversity of E. canis remains undefined. The aim of this study was to characterize E. canis in naturally infected dogs from Turkey by sequencing and phylogen...
Equine piroplasmosis (EP) is a protozoon disease caused by Babesia caballi and Theileria equi transmitted by ticks from the Ixodidae family. This study investigated the genetic heterogeneity and diversity of piroplasm genotypes using the Reverse Line Blotting (RLB) technique for piroplasm species in equids in Turkey. A total of 233 blood samples fr...
This study was carried out in order to reveal the presence and prevalence of hemotropic
Mycoplasma spp. in goats in Antalya region by nested PCR. For this purpose, a total of 157 blood samples were collected from the 3 focus of the Antalya province (Akseki, Manavgat and Serik). The animals used in the study were also examined for presence of ticks....
This study reports a novel species of Babesia in unfed Rhipicephalus sanguineus sensu lato and its phylogenetic relationship to other species. One-hundred and forty DNA samples extracted from tick pools comprising 5,403 unfed R. sanguineus s.l. ticks were screened for Babesia. Overall Babesia infection was detected in female, male, and nymph pools...
Anaplasma ovis is a widely distributed tick-borne rickettsial pathogen of sheep, goats, and wild ruminants. The aims of this study were to assess the prevalence, associations of Anaplasma ovis in sheep and goats, as well as its genetic diversity based on analysis of the msp1α gene. A total of 416 DNA samples from sheep (n = 236) and goats (n = 180)...
This study investigated possible transstadial transmission of Ehrlichia canis by Rhipicephalus sanguineus sensu lato collected from shelter dogs and the shelter grounds in Diyarbakır Province of south-eastern Turkey. Totally 225 engorged nymphs were collected from eight infected dogs with E. canis and incubated at 28°C for moulting. Unfed ticks fro...
Canine tick-borne pathogens are the source of emerging diseases and have important zoonotic relevance. Dogs play a major role in the transmission of several zoonotic tick-borne pathogens, as reservoirs and/or sentinels. To simultaneously detect Anaplasma and Ehrlichia species, a reverse line blot assay was conducted on 219 blood samples collected f...
During early lactation, dairy cows may present a transient immunosuppressive state and develop anaplasmosis caused by Anaplasma marginale. In this study, clinical anaplasmosis in dairy cattle in the Thrace region of Turkey was investigated with respect to within-herd prevalence, vertical transmission, and genetic diversity. In March and September 2...
Hemotropic mycoplasmas are emerging zoonotic pathogens that cause mild to severe hemolytic
anemia. Blood samples and thin blood smears were collected from six sheep exhibiting clinical
symptoms typical of hemolytic anemia. Coccoid and ring-shaped M. ovis were found on the
surface of erythrocytes of infected sheep. Parasitemia ranged from 0.7% to 3....
This study aimed to assess the occurrence of canine haemoplasma infection in domestic dogs and its possible trans-stadial transmission by Rhipicephalus sanguineus sensu lato (Ixodida: Ixodidae) in shelter dogs in Diyarbakır Province in southeast Turkey. Blood samples (n = 282) collected from domestic dogs were analysed by polymerase chain reaction...
Canine tick-borne apicomplexan parasites have emerged in recent years, showing a wider geographic distribution and increased global prevalence. A reverse line blot assay was performed on 219 blood samples collected from domestic dogs for simultaneous detection of all named canine piroplasm species as well as Hepatozoon canis. Ten samples hybridized...
This study investigated possible transovarial and transstadial transmission of Hepatozoon canis by Rhipicephalus sanguineus (Latreille) ticks collected from naturally infected dogs in a municipal dog shelter and the grounds of the shelter. Four hundred sixty-five engorged nymphs were collected from 16 stray dogs that were found to be infected with...
In this study, a novel Babesia sp. infecting goats was detected and its phylogenetic relationship to related species was determined. A total of 200 blood samples collected from sheep (n = 78) and goats (n = 122) were examined in the study. The V4 hypervariable region of the 18S rRNA gene of the novel Babesia sp. was amplified by PCR and analysed us...
Blood and tick samples were collected from 333 apparently healthy sheep and 257 goats as well as 10 sheep exhibiting clinical signs of babesiosis in Adana, Gaziantep, and Adiyaman Provinces in southern Turkey. Fully engorged female ticks were selected and maintained in an incubator until they oviposited. The tick carcasses and their egg masses were...
The aim of this study was to determine changes in some biochemical parameters in the healthy and infected sheeps and goats with Theileriosis. Sixty-five sheep and 67 goats were used in the study. Blood samples were tested for the presence of Theileriosis using PCR and RLB. 3.07% of sheep and 3.06% of goats blood samples were found positive with The...
Clinical cases of babesiosis were evaluated, and the frequency of bovine Babesia and Theileria parasites was determined in cattle. Blood samples and thin blood smears were collected from 23 cattle exhibiting clinical signs of babesiosis. In addition, tick and blood samples were collected from 100 apparently healthy cattle cograzing from the same ar...
Canine tick-borne parasites have emerged in recent years, showing a wider geographic distribution and increased global prevalence. In addition to their veterinary importance, domestic dogs play an important role in the transmission cycles of some agents by acting as reservoirs and sentinels. This study investigated Babesia, Theileria, Anaplasma, an...
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This study was carried out to determine the presence and frequency of Anaplasma ovis and Anaplasma phagocytophilum in small ruminants from Bingol, Elazig, Malatya and Mus provinces. A total of 422 (291 sheep and 131 goats) blood samples were collected from apparently healthy animals. To determine of A. ovis and A. phagocytophilum in small ruminants...
This study was carried out to determine the presence and frequency of Anaplasma ovis and Anaplasma phagocytophilum in small ruminants from Bingol, Elazig, Malatya and Mus provinces. A total of 422 (291 sheep and 131 goats) blood samples were collected from apparently healthy animals. To determine of A. ovis and A. phagocytophilum in small ruminants...
##Assembly-Data-START## Sequencing Technology :: Sanger dideoxy sequencing ##Assembly-Data-END##
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The present study was designed to detect the presence of tick-borne parasites (Theileria and Babesia spp.) in 196 blood samples collected from apparently healthy sheep and goats from two provinces, Punjab and Khyber Pukhtoon Khwa, in Pakistan.
Reverse line blot (RLB) assay was applied for the parasitic detection by the amplification of hypervariabl...
The occurrence and distribution of Hepatozoon species in stray dogs, and the developmental stages of Rhipicephalus sanguineus detached from the same dogs in Diyarbakır Province, Turkey is reported. A total of 328 ticks, including 133 adults (55 males and 75 females consist of 63 partially engorged and 15 fully engorged) and 195 nymphs (91 partially...
The study reports the frequency of infestation and the prevalence of tick-borne pathogens in feeding adult ticks detached from cattle in two climatic zones of the Black Sea region of Turkey. A total of 2160 adult ticks were collected during 2007-2008. Of these, 1062 were randomly selected, divided into 224 pools, and tested for the presence of bovi...
Questions
Questions (2)
Dear Colleagues,
I am serving as the Guest Editor for Special Issue entitled "Babesia and Babesiosis in Animals” and kindly invite you to contribute a paper to this special issue. If you accept our invitation, we can offer a special discount as feature paper.
The submission deadline is 15 August 2023. You may send your manuscript now or up until the deadline. Submitted papers should not be under consideration for publication elsewhere. We also encourage authors to send a short abstract or tentative title to the Editorial Office in advance (pathogens@mdpi.com or andrew.zhang@mdpi.com).
For further reading, please follow the link to the Special Issue Website at:
Submit to Special Issue, please click:
Tentive title and short abstract could be submitted here:
Pathogens is an international, open access journal of pathogens and pathogen-host interactions published monthly online by MDPI. Pathogens was covered by PubMed (PMC), Scopus and SCIE (IF: 4.531). As an international, open access journal, all published articles in Pathogens can be accessed online for free by readers worldwide. Manuscripts are peer-reviewed and a first decision provided to authors approximately 16.5 days after submission; acceptance to publication is undertaken in 3.5 days (median values for papers published in this journal in the second half of 2022). We kindly invite you to learn more about our journal at http://www.mdpi.com/journal/pathogens.
If there are any questions, please feel free to contact me or Andrew Zhang (andrew.zhang@mdpi.com).
We are looking forward to receiving your contributions.
Sezayi ÖZÜBEK
Guest Editor
LAMP amplicon syber green positive under UV light, but negative result in agarose gel. What is your opinion?
10X buffer 2,5 ul
dNTP 25 mM 0,8 ul
MgSO4 100mM 2ul
Betaine 5M 0,8 ul
Primer FIP-BIP 40pmol 0,7 ul each F3-B3 5pmol 0,7ul each
BST polymerase 1ul
nuclease free water 14 ul
Lamp condition; 62,8 C 60 min - 80 C 10 min
