Seyhan Ulusoy

T.C. Süleyman Demirel Üniversitesi | SDU · Department of Biology

The aim of this study is to fabricate streptomycin sulfate (STM)-loaded bis-chalcone/poly(methyl methacrylate) (PMMA)/poly(ethylene oxide) (PEO) based fiber mats for drug delivery and antibacterial applications. This study presents the synergetic effect of a bis-chalcone derivatives ((2E,6E)‐2,6–bis[(thiophen‐2–yl)methylene]cyclohexanone (BTC) or (...

Aim: To investigate the hypothesis that zeolites interfere with quorum-sensing (QS) systems of Chromobacterium violaceum and Pseudomonas aeruginosa by adsorbing N-acyl homoserine lactone (AHL) signal molecules. Methods: QS inhibition by zeolite 4A was investigated using an AHL-based bioreporter assay. The adsorption of the AHLs was evaluated by per...

Aim: To compare the biofilm removal efficiencies of minimally invasive and traditional chemomechanical preparations in mandibular first molars using culture method and Confocal Laser Scanning Microscopy (CLSM). Materials and Methods: Sixty caries-free mandibular first molars were embedded in acrylic blocks and divided into 2 groups: Contracted or c...

In this study, it was aimed to reveal the potential of using exopolysaccharides (EPS) obtained from Ligilactobacillus salivarius as a prebiotic that regulates chicken intestinal microbiota. Characterization of EPS obtained from L. salivarius BIS312 (EPSBIS312) and BIS722 (EPSBIS722) strains was demonstrated by high performance liquid chromatography...

ITCs are natural plant products with antibacterial, anticancer, and antioxidant properties. More than 120 different forms of ITCs have been discovered in plants, and benzyl isothiocyanate (BITC) is one of the most important bioactive components of ITCs, which may be isolated from harmless natural foods with antibacterial qualities like papaya fruit...

This study investigated the production of poly(lactic acid) (PLA) nanofibers containing cypress (CUP) essential oil (EO) via electrospinning. The nanofibers were produced from polymer solution prepared with different percentages of cypress EO. Cypress EO-containing PLA nanofibers were characterized and some mechanical and thermal properties were ex...

This study was carried out to examine some of the biological activities and physicochemical properties of the Cupressus sempervirens L. essential oil (EO). C. sempervirens L. EO was isolated by hydrodistillation. GC-MS was used to identify the compounds of the EO. The results revealed that the major compounds identified in the EO showed a predomina...

The synthesis and biomedical applications of nano/micromotors have attracted great attention. In this work, preparation and characterization of catalytic gold/poly(3,4-ethylenedioxythiophene)/platinum (Au/PEDOT/Pt) micromotors were reported to present their contribution for cancer biomarker miRNA-21 detection. Potential use of these motors for poss...

The objective of this work was to evaluate antibacterial and antibiofilm activity of Eucalyptus camaldulensis EO-loaded PLA nano-fibers. Different concentrations of E. camaldulensis EO (7.5, 10 and 15%) were incorporated into PLA nanofibers via an electrospin-ning technique. The nanofibers are characterized by Fourier transform infrared spectroscop...

Zinc oxide nanoparticles (ZnO NPs) have anticancer, antidiabetic, antibacterial, and anti-inflammatory properties in the biotechnology field. Cafestol, which is the one of the diterpene in coffee, has antimicrobial and anticancer activities. This study aimed to synthesize the cafestol–chitosan–ZnO NP system to evaluate its antibacterial activity. Z...

In this study, polypropylene (PP) nonwoven fabric which can be used as topsheet layer of an absorbent hygienic product was modified by natural based antibacterial agents. Antibacterial herbal agents (cinnamaldehyde, geraniol, phenylethyl alcohol) were sprayed by ethanol or applied by means of polylactic acid (PLA) and polycyclohexene oxide (PCHO) b...

Violacein is a natural violet pigment produced by various bacterial species, especially by the Gram-negative bacteria Chromobacterium violaceum. Violacein has antibacterial, antifungal, antioxidant and anticancer activities and has various applications in food, cosmetic, textile and pharmaceutical industries. In this study, violacein was extracted...

Many Gram-negative bacteria use N-acyl ho-moserine lactone (AHL) signal molecules to monitor their own population density and coordinate gene regulation in a process called quorum sensing (QS). Pseudomonas aeruginosa controls production of vir-ulence factors (elastase, pyocyanin or swarming mo-tility) via QS. The discovery that QS system regu-lates...

Hücreler arası iletişim sistemi (quorum sensing system (QS)), bakterilerin birbirleriyle oto-indükleyici olarak adlandırılan, küçük difüze olabilen moleküller kullanarak iletişim kurduğu bir sistemdir. Bu sistemin bakterilerde, virülans faktörlerinin üretimi de dahil olmak üzere pek çok fonksiyonu kontrol ettiği, yapılan çalışmalarla gösterilmiştir...

This study reports that quartz crystal microbalance (QCM) is used to determine the effect of chitosan derivatives obtained by chemical method on biofilm inhibition. Plasma grafted chitosan (pchito) was modified chemically with both 5-methyl-4-phenyl-thiophene-3-carboxylic acid (MFTKA) and 4-(thiophene-2-sulfonylamino)-benzoic acid (TSABA). The quar...

Background: Pseudomonas aeruginosa is an opportunistic pathogen that causes chronic infections in immunocompromised patients. The inhibition of Quorum Sensing (QS) system has been recognized as an attractive strategy for the treatment of P. aeruginosa infections. In the present study, a series of novel 2-methyl-3-[4-(substituedaminosulfonyl)phenyl]...

The aim of this work is to investigate the suitability of electrospinning for biomedical applications and to produce fast-dissolving drug delivery through uniform dextran nanofiber nonwoven maps. To prepare oral fast-dissolving drug delivery nonwoven maps via electrospinning technology, ibuprofen (Ibu) and acetylsalicylic acid (ASA) as the model dr...

In this study, wet wipes were produced for body applications with nonwoven fabrics consisting of polyester and cellulose (viscose and Tencel). Fabrics were wetted by natural-based wetting solutions (rose water, olive oil) which were functionalized by sodium alginate and natural antibacterial agents (cinnamaldehyde and geraniol) without preservative...

Background: A series of novel N-(substituted aryl/alkylcarbamothioyl)-4-[5-(4-methylphenyl)- 3-(trifluoromethyl)-1H-pyrazol-1-yl]benzene sulfonamides [SGK 318, 319, 320, 324, 327] and N-(3- substitutedaryl/alkyl-4-oxo-1,3-Thiazolidin-2-ylidene)-4-[5-(4-methylphenyl)-3-(trifluoromethyl)-1H-pyrazol-1- yl]benzenesulfonamides[SGK 328, 329, 330, 334, 33...

Anticancer Activities of Violacein Extracted From Chromobacterium violaceum ATCC 12472

Rosa damascena Mill. is a very fragrant member of Rosaceae family, exhibiting strong antioxidant and antimicrobial activity. In this study, in vitro cell cultures from Rosa damascena Mill. flower petals were established and the effects of precursor L-phenylalanine, the elicitor methyl jasmonate and light/dark treatments on the accumulation of chara...

Pek çok Gram negatif patojen bakteri çeşitli virülens faktörlerinin üretiminin genetik seviyede kontrolünü N-Açil homoserin lakton aracılı hücreler arası iletişim sistemini kullanarak sağlar. Bu nedenle bu hücreler arası iletişim sisteminin engellenmesi veya modifiye edilmesi, bu sistemi yeni terapötiklerin keşfi için cazip bir hedef haline getirme...

Objective: To investigate the effects of plant-derived phenolic compounds (i.e. caffeic acid, cinnamic acid, ferulic acid and vanillic acid) on the production of quorum sensing regulated virulence factors such as pyocyanin, biofilm formation and swarming motility of Pseudomonas aeruginosa (P. aeruginosa) isolates. Methods: Fourteen clinical P. aeru...

In this study, physical, mechanical and liquid absorption and transport characteristics of wet wipe fabrics consisting of nonwoven fabrics having different materials (cellulose and polyester combinations) and weights were investigated. Wet wipes were produced by spraying with natural based liquid contents (combinations of rose water and olive oil w...

Chitosan was modified chemically with N-(5-(4-methoxy-phenyl)-furan-2-ylmethylene)-N'-phenyl-hydrazine (NMPF). The nanofibers of chitosan were obtained by in situ electrospinning and quartz crystal microbalance (QCM) methods. The structure of the modified chitosan was characterized by Fourier transform infrared spectroscopy (FTIR), X-ray photoelect...

Objectives: The inhibitory effects of iron chelators, and FeCl3 chelation on biofilm formation and swarming motility were investigated against an opportunistic human pathogen Pseudomonas aeruginosa. Materials and methods: The inhibitory activity of 2,2'-bipyridyl, lipoic acid, kojic acid and picolinic acid on biofilm formation of P. aeruginosa s...

In this study, prepared rose oil/PLA composites with different amounts of rose oil (10, 20, 25, 40% w/v) and PLA. Antibacterial activity were investigated against Escherichia coli (ATCC 25922), Staphylococcus aureus. Thermal properties of the materials were determined with DSC and mechanical properties were evaluated with tensile test.

Cleaning process applied to microorganisms on any substance is called as sterilization. This process can be applied with different methods such as chemical (EtO), filtration (gases, liquids), temperature sterilizations etc. However, these methods have some drawbacks. Plasma sterilization is one of the rapidly developing technique in the study of di...

Forty-two natural essential and edible oils were tested for their ability to inhibit bacterial QS to create a comprehensive database of candidate inhibitor compounds which may provide significant option for treatment of bacterial infections. QS inhibitory activities of oils were screened using biomonitor organisms; Quorum Sensing Inhibitor Selector...

Antibiotics are commonly used for the treatment of microbial infections. With the widespread appearance of multi antibiotic-resistant bacteria, it is becoming increasingly more difficult to treat bacterial infections with conventional antibiotics. Thus, there is an increasing need for new strategies to cope with infectious diseases. The discovery t...

In the opportunistic pathogen Pseudomonas aeruginosa, the production of several virulence factors depends on quorum sensing (QS) involving N-acylhomoserine lactone signal molecules. In vitro studies have suggested that the QS system is crucial in the pathogenesis of P. aeruginosa. However, it is unclear whether QS systems of P. aeruginosa play the...

Antibiotics are commonly used to treat microbial infections. Due to misuse or large-scale use of antibiotics, many pathogens have gained resistance which makes antibiotic treatments ineffective. The discovery that many bacteria use quorum sensing (QS) to regulate their virulence factor and pathogenicity production makes the QS system an attractive...

form only given. In the recent days, since the epidemic spread of diseases, sterilization has become very important. Atmospheric pressure plasma jets which are used locally and effective results for sterilization procedures are popular. In this study, we produced atmospheric pressure plasma jet with helium flow and used for sterilization of Escheri...

Chitosan nano powders were modified using RF hydrazine plasma produced at low pressure (26.66Pa) with 13.56MHz frequency at a power of 100W for 30min. Characterization and investigation of the properties of plasma-modified chitosan (PMCh) and non-modified chitosan (Ch) were carried out using an optical monochromator, FTIR, florescence analysis, TGA...

The antioxidant and antibacterial activities, and total phenolic contents of Rosa damascena Mill. flower extracts (absolute, essential oil and hydrosol) were investigated. The chemical compositions of these extracts were analysed by GC-MS. Phenylethyl alcohol (78.38%) was found to be the main constituent of rose absolute, while citrenellol and gera...

In the opportunistic pathogen Pseudomonas aeruginosa, the production of several virulence factors such as elastase, rhamnolipids and pyocyanin depends on cell-to-cell signaling or quorum sensing (QS) involving N-acylhomoserine lactone (AHL) signal molecules. In vitro studies with laboratory strains and virulence studies in animals with these same s...

Many Gram-negative bacteria use N-acyl homoserine lactone signal molecules to monitor their own population density and coordinate gene regulation in a process called quorum sensing (QS). Increasing evidence implies that certain eukaryotes produce QS-inhibitory compounds. In this work, we tested 46 terrestrial plants materials for their ability to i...

Without Abstract

Acylated homoserine lactones (AHLs) are self-generated diffusible signal molecules that mediate population density dependent gene expression (quorum sensing) in a variety of Gram-negative bacteria, and several virulence genes of human pathogens are known to be controlled by AHLs. In this study, strains of Pseudomonas aeruginosa, Acinetobacter bauma...