Seyed Soheil Saeedi Saravi

Harvard Medical School | HMS · Department of Medicine

Introduction Accumulation of senescent endothelial cells with age is an important driver of vascular aging and related cardiovascular disease. However, little is known about the mechanisms and signaling pathways that underpin endothelial cell senescence. Aquaporin-1 (AQP1) has been implicated in sensing hydrogen peroxide (H2O2), which differentiall...

The failing heart is characterized by elevated levels of reactive oxygen species. We have developed an animal model of heart failure induced by chemogenetic production of oxidative stress in the heart using a recombinant adeno-associated virus (AAV9) expressing yeast D-amino acid oxidase (DAAO) targeted to cardiac myocytes. When DAAO-infected anima...

Background Aging has a remarkable effect on the cardiovascular system, and negatively causes structural and functional changes in the heart including diastolic/systolic dysfunction and left ventricular (LV) dyssynchrony, as well as, arterial stiffness which may develop to heart failure with preserved ejection fraction (HFpEF) in aged adults. We rec...

Proprotein convertase subtilisin/Kexin 9 (PCSK 9) was revealed to be a key player in the lipid metabolism and therefore in the development and progression of atherosclerosis. PCSK 9 binds to the low-density lipoprotein (LDL) receptor, induces its degradation, and increases circulating blood LDL. As a result, PCSK 9 inhibitors represent an essential...

Aging is a major risk factor for cardiovascular diseases, including thrombotic events. The gut microbiota has been implicated in the development of thrombotic risk. Plant-derived omega-3 fatty acid ɑ-linolenic acid (ALA) confers beneficial anti-platelet and anti-inflammatory effects. Hence, antithrombotic activity elicited by ALA may be partly depe...

Background and aims Early revascularization -the gold standard therapy for ischemic stroke-is often withheld in the elderly population due to high risk of complications. Thus, safe and effective preventive and therapeutic options are needed. The plant-derived omega-3-fatty-acid alpha-linolenic-acid (ALA) has emerged as a potential cardiovascular-pr...

Background and aims Treatment with proprotein convertase subtilisin-kexin type 9 inhibitors (PCSK9i), in addition to statin therapy, reduces LDL-cholesterol (LDL-c) in some patients to extremely low levels (i.e.< 20mg/dl or < 0.52 mmol/l). There is concern that at such low levels, the physiologic role of cholesterol may be impaired, e.g. the adrena...

Background: Aquaporin 1 (AQP1), a key regulator of endothelial cell function, transports hydrogen peroxide (H 2 O 2 ) into the cells. H 2 O 2 -mediated inflammation has been implicated in endothelial dysfunction, yet specific roles of AQP1-mediated signaling pathways underlying age-dependent endothelial dysfunction remains incompletely understood....

Background Aging is associated with development of cardiovascular diseases, including atherothrombosis. Aquaporin-1 (AQP1) is a water channel that also transports hydrogen peroxide (H2O2). Regarding to the role of oxidative stress in atherothrombosis, we hypothesized that AQP1 modulates aging-associated platelet/endothelial dysfunction. Methods Hu...

Background Aging is associated with alterations in the fecal microbiome composition. The microbiota-derived trimethylamine-N-oxide (TMAO) correlates with arterial thrombotic events, e.g. myocardial infarction and stroke, the leading causes of mortality worldwide. The omega-3 fatty acid (n-3 FA) α-linolenic acid (ALA) has been shown to be protective...

Background: Cancer is a dreadful disease causing thousands of deaths per year worldwide, which requires precision diagnostics and therapy. Although the selection of therapeutic regimens depends on the cancer type, chemotherapy remains sustainable treatment strategy despite some of its known side-effects. To date, a number of natural products and th...

Hydrogen peroxide (H2O2) modulates critical phosphorylation pathways in vascular endothelial cells, many of which affect endothelial nitric oxide synthase (eNOS) signal transduction. Both intracellular and extracellular sources of H2O2 have been implicated in eNOS regulation, yet the specific endothelial pathways remain incompletely understood. Her...

We believe that, in parallel to the attempts for direct blockade of the SARS-CoV-2 penetration into host cell and repurposing drugs, finding new therapeutic strategies for patients with lung injury or cardiovascular complications/coagulopathies associated with COVID-19 should be paid particular attention. Apelin or its receptor agonists are of grea...

Short Oral Presentation [PSE-NPS2020Summit/SO/A011]

Significance Nitric oxide (NO) is synthesized by the endothelial isoform of nitric oxide synthase (eNOS) in vascular endothelial cells. The high reactivity of NO undermines its accurate detection in living cells. The direct detection of the low physiological concentrations of NO that are present in endothelial cells represents a significant analyti...

We previously described a novel "chemogenetic" animal model of heart failure that recapitulates a characteristic feature commonly found in human heart failure: chronic oxidative stress. This heart failure model uses a chemogenetic approach to activate a recombinant yeast D-amino acid oxidase in rat hearts in vivo in order to generate oxidative stre...

Hydrogen peroxide (H2O2) is a stable reactive oxygen species (ROS) that modulates cell signaling pathways, including endothelial nitric oxide synthase (eNOS). In these studies, we dissected H2O2-regulated eNOS signaling pathways in endothelial cells by exploiting a new chemogenetic approach to generate and detect H2O2 in specific subcellular compar...

The aim of this study was to investigate the effects of rapamycin (rapa) and metformin (met), combined administration on testicular torsion-detorsion (T/D) injury. A total of 108 male rats were divided randomly into six groups (n = 18), control, sham-operated, T/D, T/D + met (100 mg/kg), T/D + rapa (0.25 mg/kg) and T/D + met (100 mg/kg)+rapa (0.25...

The diterpene lactone andrographolide, isolated from Andrographis paniculata, has been proven to possess several important protective biological activities, including antioxidant, anti-inflammatory, immunomodulatory, antiseptic, antimicrobial, cytotoxic, hypolipidemic, cardioprotective, hepatoprotective, and neuroprotective effects. In addition, it...

Objective(s) Rapamycin is an immunosuppressant compound with a broad spectrum of pharmaco-logical activities. In recent years, it has been used successfully to decrease ischemia-reperfusion injury in several organ systems. The purpose of the present study was to examine the effect of rapamycin on testicular ischemia-reperfusion injury. Materials a...

Statins, cholesterol lowering drugs, have been demonstrated to exert beneficial effects in other conditions such as primary and progressing neurodegenerative diseases beyond their original role. Observation that statins ameliorate the neurodegenerative diseases such as Parkinson's disease (PD), Alzheimer's disease (AD), multiple sclerosis (MS) and...

Recent investigations have been focused on understanding the role of the cerebellum in non-motor behaviors and of the cerebellar dysfunction in neurodevelopmental, neurobehavioral, and schizo-affective disorders. Non-motor behaviors, including emotion, cognition, and social behavior, seem to be modified by impairment of the cerebellar structure-fun...

Although Parkinson’s disease (PD) is considered as the second most common life threatening age-related neurodegenerative disorder, but the underlying mechanisms for pathogenesis of PD are remained to be fully found. However, a complex relationship between genetic and environmental predisposing factors are involved in progression of PD. Dopaminergic...

Nitric Oxide Synthase-Simple Enzyme-Complex Roles provides information on nitric oxide synthase, a biomolecule of key importance for the different biological systems, including central and peripheral nervous, cardiovascular, and reproductive systems. With recent links to the role of nitric oxide in the reactions that can impact cell signaling, and...

Due to a close association between depressive disorders and altered estrogen levels, this study was conducted to examine the hypothesis that antidepressant-like effect of ethinyl estradiol (EE2) in ovariectomized mice is modulated by mammalian target of rapamycin (mTOR)/nitric oxide pathways. Female mice were undergone bilateral ovariectomy and dif...

Abstract Minimal hepatic encephalopathy (MHE), which represents the early stage of this condition, is not clinically apparent and is prevalent in up to 80% of patients. The poor outcomes of MHE encouraged us to identify more simple methods for early diagnosis of MHE. To this purpose, we evaluated the contemporary manifestations of motor, cognitive...

Objective(s) An increase in nitric oxide (NO) production has been reported in cirrhotic cardiomyopathy and, portal hypertension. Since minocycline has been shown to inhibit NO overproduction, we aimed to examine its role in a rat model of CCl4-induced cirrhotic cardiovascular complications. Materials and Methods Portal pressure and inotropic respo...

This study was performed to investigate the antidepressant-like effect of minocycline in mice exposed to organophosphate pesticide malathion and possible involvement of nitric oxide/cGMP pathway in this paradigm. Mice were administered specific doses of malathion once daily for 7 consecutive days. After induction of depression, different doses of m...

Context: Acetaminophen overdose is regarded to a common cause of acute liver failure. The hepatotoxicity leads to mitochondrial oxidative stress and subsequent necrotic hepatocellular death. Objective: This study examines the protective effect of metformin on acetaminophen-induced oxidative stress, inflammation and subsequent hepatotoxicity in m...

Aim: To explore the role of mammalian target of rapamycin (mTOR) in the pathogenesis of cirrhotic cardiomyopathy and the potential of rapamycin to improve this pathologic condition. Methods: Male albino Wistar rats weighing 100-120 g were treated with tetrachloride carbon (CCl4) for 8 wk to induce cirrhosis. Subsequently, animals were administer...

AIM: To explore the role of mammalian target of rapamycin (mTOR) in the pathogenesis of cirrhotic cardiomyopathy and the potential of rapamycin to improve this pathologic condition. METHODS: Male albino Wistar rats weighing 100-120 g were treated with tetrachloride carbon (CCl4) for 8 wk to induce cirrhosis. Subsequently, animals were administered...

Objectives This study was performed to investigate the antidepressant-like effect of 17α-ethinyl estradiol (EE2) in ovariectomized (OVX) mice and the possible role of nitrergic and gamma aminobutyric acid (GABA)ergic pathways in this paradigm. Methods Bilateral ovariectomy was performed in female mice, and different doses of EE2 were intraperitonea...

We designed this experiment to evaluate the effects of nortriptyline on testicular injury after torsion/detorsion (T/D). Ninety-six adult Wistar rats were divided into six groups 16 each in control group (Group 1), sham operated (Group 2), T/D + saline (Group 3), and in groups 4-6; were administered 2, 10 and 20 mg kg(-1) , i.p. of nortriptyline 30...

The orbital hydatid cyst is a rare entity and although most of them are located in superolateral and superomedial angles of orbits, involvement of inferior sites is uncommon. We report a 12-year-old case of primary hydatid cyst situated in inferior rectus muscle which was undergone surgical removal. Magnetic resonance imaging (MRI) was used for dif...

Testicular torsion/detorsion (T/D) can induce depression in pre- and post-pubertal patients. This study was conducted to investigate the psychological impact of testicular torsion and mechanism underlying its depressive-like behaviour, as well as antidepressant-like activity of minocycline and possible involvement of nitric oxide (NO)/cGMP pathway...

Doxorubicin is an effective chemotherapeutic drug against a considerable number of malignancies. However, its toxic effects on myocardium are confirmed as major limit of utilization. PPAR-α is highly expressed in the heart, and its activation leads to an increased cardiac fatty acid oxidation and cardiomyocyte necrosis. This study was performed to...

Abortion is the termination of a pregnancy by the removal or expulsion of a fetus or embryo from the uterus, resulting in or caused by its death. Missed abortion is the most common dangerous condition in pregnancy. An abortion can occur due to maternal complications, chronic disease, endocrine disorders, abortificient drugs, radiation, heavy metals...

Background Gastric cancer accounts 8 % of the total cancer cases and 10 % of total cancer deaths worldwide. The indoleamine N-acetyl-5-methoxytryptamine, better known as melatonin, is the principal hormone produced by the pineal gland. Recently, it has shown some anticancer role in some malignancies such as breast and colon cancer; also, some of it...

The present study is an attempt to evaluate the heavy metal contamination in the marine environment of the Caspian Sea of Iran. The concentrations of zinc, chromium, cadmium, and lead were measured in water and dorsal muscle of fish sampled from 10 selected stations along Gorgan coast using atomic absorption spectrophotometry during summer of 2011....

Mycotoxins are important risks for human health due to their widespread presence in food and environment. However, feeding of infants with safe breast milk without contamination by different pollutants including mycotoxins is essential. The aim of this study is assessing the presence and values of the mycotoxins Ochratoxin A and Aflatoxin M1 in hum...

Background: Pesticides are essential in modern agricultural practices but due to their biocide activity and potential risk to the consumer, the control of pesticide residues in foods is a growing source of concern for the general population. Extensive application of such agents as organochlorine pesticides in farmlands and contemporary agricultural...

In this study, concentrations of zinc, chromium, cadmium and lead were determined in water and three species of fish sampled from 10 selected stations along Gorgan coast using atomic absorption spectrophotometery, summer 2009. Mean lead levels in water and fish samples (119.50 ± 22.24 μg/L and 113.80 ± 33.11 μg/kg) were significantly higher than th...

The antioxidant, antinociceptive and hepatoprotective effects of H(2) receptor blockers were examined with different experimental models. Antioxidant activities were determined by employing various in vitro assay systems such as 1,1-diphenyl-2-picryl hydrazyl (DPPH) radical-scavenging activity assays, reducing power determination assays, nitric oxi...

Environmental pollution is a worldwide problem, heavy metals belonging to the most important pollutants. The progress of industries has led to increased emission of pollutants into ecosystems. Southern Caspian Sea coast is one of the most important aquasystems at the eastern south of Caspian Sea, which receives effluents discharges from heavily ind...

Plants are used worldwide for the treatment of diseases, and novel drugs continue to be developed through research from plants. There are more than 20,000 species of plants used in traditional medicines, and these are all potential reservoirs for new drugs. Cucurbitapepo has been used in traditional folk medicine to treat cold and alleviate ache. P...

Isolation and identification of some potent anti-tumor compounds from medicinal plants has motivated researchers to screen different parts of plant species for the determination of anti-tumor effects. In this study, cytotoxic effects and IC(50) of specific concentrations of hydro-alcoholic extracts of fruits of Juniperus sabina and leaves of Zatari...

Sambucus ebulus is known as dwarf elder or elderberry. S. ebulus extracts are an important area in drug development with numerous pharmacological functions in the Middle East. However, their pharmacological functions have not been clearly studied. For a long time, S. ebulus has been prescribed in traditional medicines for the treatment of inflammat...

Sambucus ebulus is a native botany and exists in large amount in Iran and consists of anti-cancer substances such as ebulin (RIP-II, ribosome inactivated protein-II), fl avonoids, etc. Isolation and identifi cation of some potent anti-tumor compounds from medicinal plants, has motivated researchers to screen different parts of plant species for ant...

Purpose: Vegetables are the major source of nitrate and nitrite in human diet. Because of the potential health hazards of high intake of nitrate and nitrite, the concentration of these two ions in vegetables has been measured in many countries. The aim of this research was to determine the concentration of nitrate and nitrite, and comparison betwee...

Purpose: Sambucus ebulus, Iranian native botany, consists of anticancer substances including ebulin and flavonoids. In this study, anti-inflammatory, toxic effects, biochemical and pathological analysis in presence of vitamins C and E, and cytotoxicity of 3 extracts of S. ebulus were evaluated. Methods: Anti-inflammatory activity of n-hexane, metha...

In this study, the concentrations of lindane residues (organochlorine pesticides) were analyzed in samples of cultivated cucumbers (Cucumis sativus L.) and four species of most consumed fish (Sefid, Koli, Kilca and Kafal fish). Samples of cucumber were collected from five sites in Sari city (north, south, east, west and central areas) and samples o...

Toxic effects of ethyl acetate extract of Sambucus ebulus and its protective role of vitamins C and E on its pathological and biochemical disorders was evaluated. Male mice were administered with specific doses of vitamin C, E, C+E and ethyl acetate extract of S. ebulus. Serum BUN and creatinine, and nephro- and hepatopathology after administration...

The importance of trace elements in chronic inflammatory diseases is related to their cofactor role in immune system functions and in different metabolic processes in articular tissues. The aim of this study was to compare serum levels of Cu, Zn and Zn/Cu ratio in Rheumatoid Arthritis (RA) patients with healthy volunteers in Sari Rheumatology clini...

Farmers use mancozeb against cucumber pests, such as Perono spoxa, in large amounts without consideration of potential chronic human health hazards resulting from pesticide accumulation in food and fruits. Considering the necessity of food for humans and importance of fruit in daily diet, as well as the determination of pesticide residues in food a...

Studies showed significant disturbances of electromagnetic fields on metabolism of carbohydrate, lipid, proteins, as well as enzyme activities. In this investigation, the effect of whole-body magnetic field (MF) on glucose and cortisol levels was studied on 30 adult male Guinea pigs. Guinea pigs were exposed to sinusoidal 5 Hz, 0.015 µT and 50 Hz,...

Environmental pollution is a global problem with heavy metals being prominent pollutants. Industrialization has led to increased emissions of pollutants into ecosystems. Gorgan coast is one of the most important coasts at the eastern south of the Caspian Sea. In this investigation, the accumulation of four heavy metals; zinc (Zn), chromium (Cr), ca...

In this study, we investigate the toxic effects of cobalt chloride on some hematological factors of the carp Cyprinus carpio, such as white blood cell count, red blood cell count, hemoglobin, hematocrit, mean corpuscular volume, mean corpuscular hemoglobin, and mean corpuscular hemoglobin concentration. At first, LC50 of cobalt in C. carpio was mea...

Sambucus ebulus is a native botany and exists in large amount in Iran and consists of anti-cancer substances such as ebulin (RIP-II, ribosome inactivated protein-II), flavonoids, etc. Isolation and identification of some potent anti-tumor compounds from medicinal plants, has motivated researchers to screen different parts of plant species for anti-...

The accumulation of four heavy metals; zinc (Zn), chromium (Cr), cadmium (Cd), and lead (Pb) in sediment collected from 10 stations along the Gorgan coast, Caspian Sea (Iran) were studied. Gorgan coast receives effluent discharges from heavily industrialized and highly populated settlements. The sediment was sampled and analyzed for heavy metals by...

The concentrations of chromium (Cr), cadmium (Cd), and lead (Pb) were determined in three species of fish at the fishing stations of Gorgan coast, Caspian Sea, Iran. The heavy metal content in dorsal muscles of fish was estimated by atomic absorption spectroscopy. Results of this study showed that the mean Pb levels in all fish samples were signifi...

Introduction: Farmers consciously and unconsciously use Mancozob against cucumber pests, such as Perono spoxa species, in large amount; and they don't consider to probable chronic human health hazards of pesticides accumulated in food and fruits. Considering the necessity of food for human beings and important position of fruit in daily diet and al...

The trophic state of the Anzali wetland was determined by nutrient analysis, indicating an alarming hypertrophic state. The Anzali wetland is environmentally and economically one of the most important ecosystems that is located in north-west Iran. This wetland was registered as a Ramsar site in September 1975, but due to many problems, particularly...

The trophic state of the Anzali wetland was determined by nutrient analysis, indicating an alarming hypertrophic state. The Anzali wetland is environmentally and economically one of the most important ecosystems that is located in north-west Iran. This wetland was registered as a Ramsar site in September 1975, but due to many problems, particularl...

Changes in the copper (Cu) and zinc (Zn) concentrations have been reported previously in ischemic cardiomyopathy (ISCMP). Due to controversial results, the aims of this study were to compare levels of Cu, Zn, and Zn/Cu ratio of ISCMP patients with healthy volunteers and also to investigate the possible relationship between trace elements status in...

The tea bush (Comellia sinensis) is grown commercially in a large number of countries. Tea is a beverage, which consists of processed and dried tea leaves and is one of the most popular global drinks. Tea contains some heavy metals, such as Pb and Cd, which exert adverse effects on human health. The aim of this study was to determine the Pb and Cd...