Servio Urdaneta-Morales

Servio Urdaneta-Morales
Central University of Venezuela | UCV · Instituto de Zoología Tropical (IZT)

Ph. D.

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Publications (57)
Article
Full-text available
Chagas’ disease or American Trypanosomiasis, caused by Trypanosoma (Schizotrypanum) cruzi (Kinetoplastida, Trypanosomatidae) is a pandemic parasitosis spreaded alongside the Neotropic; in Venezuela is endemic in all States except in Delta Amacuro and Nueva Esparta. The aim of this descriptive, epidemiological, field and cross-section study was to d...
Book
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Organized and sponsored by the Nencki Institute of Experimental Biology in Warsaw, with the assistance of the Committee on Cell Biology, Polish Academy of Sciences (Member of ECBO) and the Protozoological Section of the Polish Zoological Society on behalf of the International Commission of Protozoology.
Article
Full-text available
The aim of this study, done through the examination of specialized articles, Google Scholar data and results of field work and laboratory research, was to summarize the following objectives: to stablish the Venezuelan mammal species host for Trypanosoma (Schizotrypanum) cruzi, the causative of Chagas' disease; to identify the cinegetic vertebrate s...
Article
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The unprecedented emergence of important public health and veterinary zoonoses is usually a result of exponential population growth and globalization of human activities. I characterized Chagas´ disease as an emergent zoonosis in the Caracas Valley (Venezuela) due to the following findings: the presence of reservoirs (Didelphis marsupialis, Rattus...
Article
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RESUMEN Por cuanto el molusco Achatina fulica nativo del África es vector de helmintos, pero su relación con protozoarios y bacterias es poco conocida, decidimos estudiar las excretas de 1.200 ejemplares capturados en los estados Anzoátegui, Monagas, Sucre y Nueva Esparta, del noreste de Venezuela. Su moco pedal y heces mostraron infección por los...
Article
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The mollusk Achatina fulica, native to Eastern Equatorial Africa, has been incriminated as a carrier or vector of helminthes. Nevertheless, information in the literature as regards its status as a carrier for bacteria is scarce, and we could find no reference at all for its relation to protozoa. We studied microscopically the excreta from 1200 snai...
Article
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Trypanosoma cruzi, etiological agent of Chagas' disease, was isolated from armadillos (Dasypus novemcinctus novemcinctus) captured in rural communities Northeastern Venezuela from Nueva Esparta State (no endemic for Chagas' disease), Monagas and Anzoátegui States (endemics). The isolates, genetically typed by PCR-RFLP as belonging to the TcIII DTU,...
Article
Full-text available
Trypanosoma cruzi causes a pan-infection, Chagas disease, in American mammals through fecal transmission by triatomine insects, resulting in an acute phase parasitemia with intracellularity mainly in the myocells and cells of the central nervous system (CNS).The parasites, due to the immune response, then decrease in number, characteristic of the l...
Data
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T. cruzi genotypes from bugs of different States in Venezuela. (PDF)
Data
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T. cruzi genotypes from mammals of different States in Venezuela. (PDF)
Data
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T. cruzi genotypes from human of different States in Venezuela. (PDF)
Article
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Chagas disease is an endemic zoonosis native to the Americas and is caused by the kinetoplastid protozoan parasite Trypanosoma cruzi. The parasite is also highly genetically diverse, with six discrete typing units (DTUs) reported TcI - TcVI. These DTUs broadly correlate with several epidemiogical, ecological and pathological features of Chagas dise...
Article
Full-text available
Chagas disease is an endemic zoonosis native to the Americas and is caused by the kinetoplastid protozoan parasite Trypanosoma cruzi. The parasite is also highly genetically diverse, with six discrete typing units (DTUs) reported TcI-TcVI. These DTUs broadly correlate with several epidemiogical, ecological and pathological features of Chagas diseas...
Article
Full-text available
The authors report the presence of Eratyrus mucronatus (Stal, 1859) (Hemiptera, Reduviidae, Triatominae) infected with Trypanosoma cruzi, in an urban peridomiciliar area (Universidad de Oriente, Núcleo de Anzoátegui, Anzoátegui state, Venezuela), what represents, to our knowledge, the first report of the species for this state. The epidemiological...
Article
Full-text available
There are few reports of Coccus nucifera (Palmae) infestation by triatomines (Hemiptera, Reduviidae, Triatominae), vectors of Trypanosoma cruzi (Kinetoplastida: Trypanosomatidae), the causal agent of American Trypanosomiasis. The aim of this study was to determine if this palm is an appropriate ecotope for Rhodnius prolixus and Triatoma maculata, t...
Article
Full-text available
Trypanosoma cruzi (Kinetoplastida, Trypanosomatidae) a clonal parasite, presents a considerable genetic heterogeneity, manifested in three lineages, defined as TcI, TcII and Z3, the latter present more frequently in the equatorial region and over the Amazon basin. In Venezuela there is a predominance of TcI genotype. An interesting aspect is the di...
Article
Full-text available
Trypanosoma cruzi causal agent of Chagas' disease is a paninfective parasite of mammals transmitted through skin fecal contamination by Triatominae vectors. Studies of alternative routes for infection are scarce; therefore, eye infection should be important, because of the eye's high blood irrigation and brain proximity, as port of entry of the par...
Article
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Trypanosoma cruzi causes Chagas' disease, a systemic infection that affects cells of meso-, endo-, and ectodermic origin. However, as far as we know, the presence of T. cruzi stages in bone has not been reported previously, and it has scarcely been investigated in cartilage. We inoculated 7- and 20-day-old (8 and 15 g) NMRI albino mice i.p. with me...
Article
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Trypanosoma cruzi, an ubiquitous obligatory intracellular hemoflagelate, is responsible for Chagas's disease in mammals. Macrophages and adipocytes have been considered as to be early parasite invasion targets; even when historically their tissue behavior has usually been studied in muscles and digestive and nervous structures which are generally d...
Article
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To understand the interaction of Trypanosoma cruzi with caviomorph rodents, which supposedly have an ancient co-evolutionary history with this parasite, experimental infection of laboratory reared Trichomys apereoides with several isolates of both genotypes of the parasite was studied. Parasitemia, pattern of hematic cells, specific humoral immune...
Article
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Trypanosoma cruzi colonizes the anal scent glands of Didelphis marsupialis opossum, differentiating into metatryposmastigotes, which affect mammals through contamination in the same way as do metacyclics formed in the stomach of the triatomine vector. Meta-tryposmastigotes harvested from anal glands of D. marsupialis and from the intestine of Panst...
Article
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Trypanosoma cruzi is usually transmitted by contact with the excreta of infected Triatominae; among non-vectorial infections, direct transmission through coitus has been proposed. We investigated this possibility by instilling, through the external meatus of the vagina and the penis of previously anesthetized NMRI albino mice, blood of mice infecte...
Article
Full-text available
Trypanosoma cruzi can develop through an extracellular cycle in the anal scent glands of opossums. Blood from infected opossums (Didelphis marsupialis) was inoculated directly into the lumen of the anal glands of healthy opossums, one of which developed parasitemia with detectable amastigotes in the heart, without anal gland colonisation. Control o...
Article
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Direct blood examination and xenodiagnosis of 47 synanthropic rodents (Rattus rattus, R. norvegicus, Mus musculus) captured in the valley of Caracas, Venezuela, revealed trypanosomal infections in 12 R. rattus, 10 with T. lewisi and 2 with T. cruzi. Of the latter the course of parasitemia, the pleomorphism of the bloodstream trypomastigotes, tissue...
Article
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Opossums (Didelphis marsupialis) captured in intensely urbanized areas of the city of Caracas, Venezuela, were found infected with Trypanosoma cruzi. The developmental cycle of trypomastigote-epimastigote-metacyclic infective trypomastigote, usually occurring in the intestine of the triatomine vector, was taking place in the anal odoriferous glands...
Article
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The histopathological alterations produced in NMRI strain mice by isolates of Trypanosoma cruzi from Didelphis marsupialis captured near human dwellings in the valley of Caracas, Venezuela are described. The donor opossums showed pseudocysts and amastigotes and trypomastigotes only in the heart muscle, and few areas of discrete inflammations and ly...
Article
Routine transmission electron microscopy of Trypanosoma rangeli culture forms reveals a common ultrastructural pattern with other trypanosomatids. Connections of nuclear envelope and endoplasmatic reticulum are described. Profuse stacked cisternae of rough endoplasmatic reticulum and distended cisternae of smooth endoplasmatic reticulum are shown....
Article
Full-text available
A genetic analysis of a set of Trypanosoma cruzi and Trypanosoma rangeli stocks was performed by two combined approaches, namely multilocus enzyme electrophoresis (MLEE) and labeling by DNA probes. A considerable genetic variability was evidenced within each of the two species. Since the upper level of resolution of the isoenzyme method was reached...
Article
Full-text available
Direct blood examination and xenodiagnosis of 45 sylvatic, peridomestic or domestic mammals from the Caracas valley, Venezuela, revealed trypanosome infection in six of the 24 opossums, Didelphis marsupialis, collected in urban areas. Isolates were successfully made of trypanosomes from four of the opossums, using the parasites which developed in R...
Article
Full-text available
Meta-trypomastigotes of Trypanosoma rangeli Tejera, 1920, harvested from LIT medium, were inoculated i.p. or s.c. into 6, 16, and 26 g NMRI mice, these representing increasing degrees of immunological maturity. In all cases, similar pleomorphic patterns were observed. Four morphobiometrically differentiable types of trypanosome were encountered in...
Article
Direct blood examination and xenodiagnosis of 45 sylvatic, peridomestic or domestic mammals from the Caracas valley, Venezuela, revealed trypanosome infection in six of the 24 opossums, Didelphis marsupialis, collected in urban areas. Isolates were successfully made of trypanosomes from four of the opossums, using the parasites which developed in R...
Article
Full-text available
The histopathological alterations produced in white mice (NMRI strain) by isolates of Trypanosoma (Schizotrypanum) cruzi from Panstrongylus geniculatus captured in dwellings of the city of Caracas, Venezuela are reported. The heart, skeletal muscle, liver, spleen, duodenum, colon, lung, and brain were parasitized by all the isolates. All showed str...
Article
For evaluation of the possible pathogenicity of Trypanosoma (Herpetosoma) rangeli Tejera, 1920 to the triatomid vector, first-stage nymphs of laboratory-bred insects were engorged upon albino mice showing average parasitemias of 2 x 10(6) and 2 x 10(5) trypanosomes/ml blood. The vector strains were: Rhodnius prolixus ("New" strain), Triatoma pallid...
Article
Full-text available
To study the influence of host age, inoculum size, and route of infection on Trypanosoma (Herpetosoma) rangeli, 12 lots of 6.0 g albino mice (NMRI strain) were infected up. with from 25x101 to 25x106 trypomastigotes/gram body weight harvested from LIT medium. The lower inocula produced low but persistent parasitemias, while the higher inocula produ...
Article
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Ratones machos (cepa NMRI) de 3 y 5 gr, inoculados i.p. con 8 x 106 y 9 x 104 metatripo- mastigotes/gr cosechados de cultivo LIT de 12 días de repique de la cepa "Perro-82" de Trypanosoma rangeli fueron procesados, em determinados períodos postinoculación, para seccionar y teñir con hematoxilina-eosina y Giemsa-colofonio trozos de corozón, hígado,...
Article
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El método, sitio y estadio de multiplicación de Trypanosoma (Herpetosoma) rangeli Tejera, 1920 no había sido determinado hasta ahora. Nosotros hemos observado numerosos nidos o pseudoquistes intracelulares que contienen amastigotes y tripomastigotes de este parásito en el corazón, hígado y bazo de ratones albinos machos de 5,0 g de peso (cepa NMRI)...
Article
Full-text available
The Neotropical mammalian parasite Trypanosoma (Herpetosoma) rangeli Tejera, 1920 is difficult to study due to the scarcity of blood forms in the vertebrate host. High and persistent parasitemias (up to 7 times the original inoculum at the peak, and persisting for up to 2 wk) were obtained by i.p. inoculation of infant (6.0 g) male white mice (NMRI...
Article
The sequential variation in the body type of trypomastigotes of the 'Brazil' strain of Trypanosoma cruzi was followed in 4 experimental models: Cultures of Vero fibroblasts incubated at 37 degrees C. Cultures of fish epithelial cells (Pimephales promelas) incubated at 37 degrees C. Blood of C3H mice inoculated with trypomastigotes from 37 degrees C...
Article
Full-text available
Trypomastigotes of the 'Brazil' strain of Trypanosoma cruzi were cultured in Vero and fish (Pimephales promelas) cells at 30 and 37 C. Those harvested from 37 C Vero cell cultures killed all inoculated C3H mice no matter how long the cultures had been maintained. Those harvested from 120-day 30 C Vero and fish cultures killed only 1 out of 10 mice...
Article
Full-text available
Virulent trypomastigotes of the Y strain of Trypanosoma cruzi were administered to Tropidurus hispidus, Ameiva ameiva, Cnemidophorus lemniscatus, Polychrus marmoratus, and Phyllodactylus ventralis (Sauria). Intraperitoneal and subcutaneous inoculations of lizards with mouse blood or with feces of infected Rhodnius prolixus (Reduviidae, Triatominae)...
Article
Full-text available
Experimental infections of three hematophagous arthropods (Rhodnius prolixus, Aedes aegypti, and Culex pipiens) with a trypanosome of the Trypanosoma rotatorium complex found in the frogs Hyla crepitans and Leptodactylus insularum revealed that A. aegypti is a good host for the flagellate; the course of development in the intestinal tract of the mo...
Article
Thesis--University of Georgia, 1979. Directed by William L. Hanson. Includes bibliographical references (leaves 44-53).
Article
SYNOPSIS. Two hundred and fifty-nine specimens of the sea urchins Echinometra lucunter (201), Diadema antillarum (52), Tripneustes ventricosus (5) and Eucidaris tribuloides (1), collected on the littoral of the Federal District of Venezuela, were examined for intestinal ciliates. Iron hematoxylin and silver-impregnation staining technics permitted...

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Projects (2)
Project
Study the genetic variability of Aedes aegypti from different regions of Paraguay. Determine the genetic profile of Phlebotomists related to the transmission of Leishmaniasis in Paraguay. Determine genetic and epidemiological characteristics of Triatomines
Project
Mediante el estudio de ms especializados y trabajos de campo y de lab. se determinarán: las especies de mamíferos venezolanos hospedadores de T. cruzi; las especies de vertebrados capturados por poblaciones indígenas y mestizas neotropicales que se comportan como cinegéticas, caracterizadas como aquellas que son capturadas fundamentalmente para la obtención de carne que provee sus necesidades proteicas y la elaboración de medicamentos zooterapéuticos; comportamiento parasitológico de aislados del parasito obtenidos de estos mamíferos y los riesgos epidemiológicos que representan sus hábitos de uso en la infección y transmisión del parasito.