Sergii V Romanenko

University of Western Australia | UWA · School of Physics

Modelling rare neurogenetic diseases to develop new therapeutic strategies is highly challenging. The use of human-induced pluripotent stem cells (hiPSCs) is a powerful approach to obtain specialized cells from patients. For hereditary peripheral neuropathies, such as Charcot-Marie-Tooth disease (CMT) Type II, spinal motor neurons (MNs) are impaire...

MMW radiation can be used to focally deposit energy into biological tissue, which converts in it into thermal energy due to high absorption properties, specifically of water. As a result, it can affect the functionality of some biological tissues. Neuronal tissue is one of the most susceptible types due to its high sensitivity to environmental chan...

Triple-negative breast cancers (TNBC) are highly aggressive malignancies comprising ~15-20 % of all breast cancers. TNBCs quickly acquire drug resistance mechanisms and are inherently resistant to radiotherapy, which significantly limits therapeutic options. Thus, there is a need to develop more selective and efficient therapeutic approaches to tar...

There is evidence that millimeter waves (MMWs) can have an impact on cellular function, including neurons. Earlier in vitro studies have shown that exposure levels well below the recommended safe limit of 1 mW/cm2 cause changes in the action potential (AP) firing rate, resting potential, and AP pulse shape of sensory neurons in leech preparations a...

Due to new applications such as wireless communications, security scanning, and imaging the presence of artificially generated high frequency (30-300 GHz) millimetre-wave (MMW) signals in the environment is increasing. Although safe exposure levels have been set by studies involving direct thermal damage to tissue, there is evidence that MMWs can h...

Heating is the most obvious effect that exposure to millimeter-wave-to-terahertz (MMW-THz) radiation has on living tissue. It is expected that the resulting increase in temperature causes up regulation of cellular mechanisms, e.g. increased rate of action potential (AP) generation. However, some studies have demonstrated the opposite. Here we show,...

Since regular radio broadcasts started in the 1920s, the exposure to humanmade electromagnetic fields has steadily increased. These days we are not only exposed to radio waves but also other frequencies from a variety of sources, mainly from communication and security devices. Considering that nearly all biological systems interact with electromagn...

Determining the absorption of MMW in living tissue is a complex problem due to tissue inhomogeneity. As the sample size and tissue elementary structural unit becomes smaller, e.g. single cells, the measurements with contemporary techniques get more challenging. Also, MMW and THz radiation can affect the functionality of the biological sample, thus...

Nanosecond pulsed electric fields (nsPEFs) have a variety of applications in the biomedical and biotechnology industries. Cancer treatment has been at the forefront of investigations thus far as nsPEFs permeabilize cellular and intracellular membranes leading to apoptosis and necrosis. nsPEFs may also influence ion channel gating and have the poten...

This study demonstrates effect of 60 GHz radiation applied in a pulsed mode to the finite neuronal network. Short powerful pulses of 50 to 300 msec duration were applied to leech neuronal ganglion and electrophysiological activity of the Retzius cells were recorded via sharp electrodes. Pulses were applied with respect to the natural activity of th...

There is a constant and growing demand for new methods to treat drug-resistant neurological disease. Ultrashort pulsed electric fields are a new tool that has shown some promise for electrically stimulating neurons and other excitable cells. Thus far, most studies have focused on the application of these short-lived pulsed electric fields to neuron...

The effect of high power nanosecond pulsed electric fields (nsPEF) was examined on the complex neuronal assemblies of intact leech ganglia. The medicinal leech midbody ganglion is a convenient experimental preparation that represents a real neuronal network with neuronal diversity and glial cells. 10 ns pulses of different intensities ranging from...

The effect of high power nanosecond pulsed electric fields (nsPEF) was examined on the complex neuronal assemblies of intact leech ganglia. The medicinal leech midbody ganglion is a convenient experimental preparation that represents a real neuronal network with neuronal diversity and glial cells. 10 ns pulses of different intensities ranging from...

Many of today's radiofrequency-emitting devices in telecommunication, telemedicine, transportation safety, and security/military applications use the millimeter-wave (MMW) band (30-300 GHz). To evaluate the biological safety and possible applications of this radiofrequency band for neuroscience and neurology, we have investigated the physiological...

Le projet concerne l’utilisation de champs électromagnétique pulsé nanoseconde (nsPEF) comme neuro-stimulation thérapeutique. L’objectif est de déterminer si les nsPEFs ont le pouvoir d’activer des cellules excitables comme les neurones, dans un modèle de sangsue in vivo, sans engendrer de perméabilisation ou mort cellulaire. Des techniques d’élect...

The possible effects of millimeter waves (MMWs) on biological objects is becoming an increasingly important issue due to development of technologies for telecommunication, telemedicine and security/military applications operating at this extremely high radiofrequency band (30-300 GHz). Specifically, the safety limit of MMW irradiation of neuronal t...

Possible effects of millimeter waves(MMWs) on biological objectsbecomes an increasingly important issue due to development of technologies for telecommunication, telemedicine and security/military applications operating at this extremely high radiofrequency band. Specifically, the safety limits of MMW irradiationof neuronal tissueneed to be evaluat...

The use of electrically-induced neuromodulation has grown in importance in the treatment of multiple neurological disorders such as Parkinson's disease, dystonia, epilepsy, chronic pain, cluster headaches and others. While electrical current can be applied locally, it requires placing stimulation electrodes in direct contact with the neural tissue....

The functioning features of TRPV1 channels, which are expressed in dorsal root ganglion neurons somas of small (25 mkm and less) and medium (35-25 mkm) sizes as well to their influence on free calcium intracellular concentration oscillations in these neurons at their excitation, was investigated. All calcium transients were measured with calcium se...

This review is devoted to the action of amyloid-beta peptide on the functional activity of intracellular and plasmalemmal calcium-regulated structures in cultured hyppocampal neurons: mitochondria and voltage-gated calcium channels. A comparative analysis of relative changes of plasmalemmal structures in such neurodegenerative diseases as Alzheimer...

In rat neurons of the dorsal root ganglia (DRG) with mid- (35 to 25 μm) and small-sized (less than 25 μm) somata, we studied calcium transients induced by application of capsaicin (selective agonist of TRPV1 channels) under conditions of the development of other calcium transients caused by preliminary depolarization of the plasma membrane of these...

We studied the effect of gabapentin (an agent similar, in its molecular structure, to gamma-aminobutyric acid, GABA) on depolarization-evoked calcium transients in small, mid-sized, and large (diameter of the soma up to 25, 25 to 35, and 35 μm or more, respectively) neurons of the dorsal-root ganglia (DRGs) of rats with experimental streptozotocin-...

In our experiments on rat dorsal root ganglia (DRG) neurons, we studied the effects of an antiepileptic agent, gabapentin, on calcium transients evoked by depolarization of the membrane using the fluorescence calciumsensitive dye Fura-2/AM. Application of gabapentin to neurons with large-diameter somata practically did not change the characteristic...