Sergey PospelovPoltava State Agrarian Academy · Agriculture and Agrochemistry
Sergey Pospelov
Professor (full)
About
56
Publications
15,801
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11
Citations
Introduction
The area of scientific interests is the study of phytohemagglutinins (lectins) in plants and soil. He developed methods for determining the activity of lectins in soil and plants, I study the dynamics of the activity of phytolectins in the ontogenesis of plants, including medicinal plants, cereals.
For more than 25 years I have been studying the biology and cultivation of Echinacea in Ukraine (Echinacea purpurea, Echinacea pallida). As a result, technologies have been developed for the cultivation and multifunctional use of echinacea for medicinal plant growing, animal feeding (like a phytogenic), beekeeping, and for growing seeds.
A study of the biological activity of echinacea extracts in relation to plant objects is being carried out.
Research experience
July 2020 - present
September 2014 - February 2021
January 2014 - December 2019
Position
- Doctor of Science
Description
- The doctoral dissertation: "The scientific basis of the establishment of productive agrocenoses of the Echinacea Moench. in the minds of Left-bank Ukraine"
Education
September 1978 - January 1983
Publications
Publications (56)
The results of studies of Medicinal Herbs & Spices are given. The features of their introduction, biology, breeding, physiology and phytochemistry, propagation and cultivation, use in medicine and industry was considered.
Purpose. To study morphometric parameters of fruits (cypselae) of purple coneflower Echinacea purpurea (L.) Moench) of ‘Zirka Mykoly Vavylova’ cultivar and pale coneflower (Echinacea pallida (Nutt.) Nutt) of ‘Krasunia Prerii’ cultivar and determine the impact of agro-meteorological factors on their performance according to long-term data. Methods....
The bibliography "Echinacea in Ukraine" collected literature (monographs, scientific articles, patents, conference proceedings, published in the newspapers) for the period from 1915 to 2012. The book contains information about 1783 bibliographic sources, the authors who published their research in journals in Ukraine
The results of studies of medicinal plants are given. The peculiarity their biology, physiology and phytochemistry, reproduction and cultivation, use in medicine and industry was considered.
On the basis of long-term researches of Purple Coneflower (Echinacea purpurea (L.) Moench) variety «Zirka Mykoly Vavylova» and Pale Coneflower (Echinacea pallida (Nutt.) variety «Krasunja Preriy» for the first time ever there were developed and patented the methods for determining the efficiency of plants in pregenesic period of ontogeny. The found...
The collection of the Ninth International Scientific and Practical Conference “Medicinal Herbs: from past experience to new technologies” presents the results of the investigations of medicinal plants, especially their introduction, biology, breeding, physiology and phytochemistry, propagation and cultivation, pharmacy, use in agriculture and indus...
The book of the Eighth International Scientific and Practical Conference “Medicinal Herbs: from past experience to new technologies” presents the results of the investigations of medicinal plants, especially their introduction, biology, breeding, physiology and phytochemistry, propagation and cultivation, pharmacy, use in agriculture and industry.
Presented are the experimental data on the effect of biofungicides on seed infection and mycoflora of winter wheat seeds of varieties ‘Levada’ and ‘Podolyanka’. The phytostudy which was conducted allowed to determine the presence of micromycetes such as Alternaria alternata (infection rate 6.8–19.5 %), Tilletia caries (1–2 %), Fusarium spp . (0.5–3...
The results of many years of field research on the effect of stocking density of different types of Echinacea spp . on its productivity are discussed. It was found that when the Echinacea purpurea crops were thickened, the collected raw material had a high percentage (more than 50%) of stems, which negatively affected its quality. An increase in th...
As a result of experiments it was established that, besides other protein compounds, winter wheat (Triti-cum aestivum L.) contains lectins, which are able to bind reversibly to carbohydrates, oligosaccharides and polysaccharides. Their role at all levels of the living organism organization, communication and signalling processes is proved. Winter w...
It has been established that many medicinal plants of the flora of Ukraine contain lectins of high activity. Extracts from St. John's wort (Hypericum perforatum L.), dwarf everlast (Helichrysum arenarium L.), common marigold (Calendula officinalis L.), common yarrow (Achillea millefolium L.) and seeds of common sea buckthorn (Hippophae rhamnoides L...
The collection of the Seventh International Scientific and Practical Conference “Medicinal Herbs: from past experience to new technologies” presents the results of the investigations of medicinal plants, especially their introduction, biology, breeding, physiology and phytochemistry, propagation and cultivation, use in medicine and industry.
У збір...
The results of investigations of the effect of damage seeds of Echinacea purpurea (L.) Moench. and Echinacea pallida (Nutt.) Nutt. on crop quality are presented. It is established that damage to the surface of the pericarp during harvesting in the future leads to a significant decrease in seed quality and development of seedlings. The analysis of p...
It is established that purple coneflower (Echinacea purpurea (L.) Moench) and pale purple coneflower (Echinacea pallida (Nutt.) Nutt.) contains specific protein compounds - lectins, which have the ability to reversibly interact with carbohydrates, and exhibit high biological activity at the cellular and organism levels. An original methodology of h...
The article covers biological methods (biopesticides) for the protection of medicinal crops against pests. Some of the disadvantages and requirements for the production practice of the studied type of crop pro-duction are indicated. The main types of harmful insects of medicinal plants are specified and classified. The list of some preparations bas...
In the conditions of industrial crops on the basis of long-term data, the interrelation of agrometeorological factors on the growth and development of the echinacea of the second year of vegetation was studied. It was found that the mass of the aboveground part and the root system was significantly dependent on the amount of precipitation from the...
The results of studies of Medicinal Herbs & Spices are given. The features of their introduction, biology, breeding, physiology and phytochemistry, propagation and cultivation, use in medicine and industry was considered.
The scientific article outlines the constituent areas of the practical use of lectins; The main achievements of domestic and foreign scientists in the study of phytolectins are analyzed; attention is focused on the key aspects and directions of the development of lectinology. The author of the article emphasizes the need for further research of phy...
A method for determining the hemagglutinin activity of chaenomeles lectins by extraction of a plant material of 0.05 n HCl in a ratio of 1:20, filtering, protein concentration, and agglutination reaction in immunological plates in buffer systems, characterized in that the determination is carried out at a pH of the buffer system 7.0-7.5.
The biological activity of dry extracts of Echinacea purpurea, obtained from the aboveground mass and roots with rhizomes, was investigated. It was found that they showed physiological activity in relation to the systems of bioassays (sprouting seeds of lettuce, spring barley).
Extracts of echinacea represent a interest as like a growing factor (stimulant), also as biocorrector of plant origin, which can to use in agriculture.
It sets out the historical background of the emergence of the first Ukrainian institution that deals with the system studying and cultivation of medicinal plants - the Experimental Station of the Institute of Medicinal Plants of Agroecology and Environmental NAAS. Consider its role in the development of national medicinal crop. Shown iconic persona...
Different substrates for growing seedlings of Hypericum perforatum L. were studied. It was found that the addition of peat substrates and / or compost positive specimens affected to seedling development. After a 50-60 day stem height was 3.7-4.5 cm, which developed 7,4-8,5 leaves. Stem mass reaches 14,7-18,4 mg, the root system - 15,0-19,4 mg. As s...
The data of experimental investigations of morphometric parameters purple coneflower (Echinacea purpurea (L.) Moench.) and pale purple coneflower (Echinacea pallida (Nutt.) Nutt.) pregenerative period of ntogenesis. It was found that during the first 1.5-2 months vegetation aerial part of echinacea developed much slower than in subsequent months. L...
Plant residues and processing of medicinal plant products contain biologically active substances, among which are lectins. We are exploring the possibility of producing these unique protein compounds.Technological schemes can be used for further industrial application development. Research of fungistatic and growth-stimulating abilities lectin-cont...
Long-term data of studying the dynamics of accumulation hydroxycinnamic acid derivative (HCAD) in Echinacea purpurea (L.) Moench and Echinacea pallida (Nutt.) Nutt. In the conditions of Ukraine are presented. Plants of pre- generative period of ontogenesis most HCAD accumulated in the leaves of Echinacea purpurea (3,38-4,49%) and Echinacea pallida...
The conference in honor of the 100th anniversary of the Echinacea research in Ukraine.The results of studies of medicinal herbs and spices are given. The peculiarity their biology, physiology and phytochemistry, reproduction and cultivation, use in medicine and industry was considered.
The systematic analysis of the literature on diseases of medicinal plants valerian (Valeriana officinalis L.) are shown. It was found that it affected micropathogens fungal, bacterial and viral origin, the most harmful are leaf spot, root rot, powdery mildew, rust and viral diseases. They can significantly reduce the yield of rhizomes and roots, th...
This paper shows the results of two year research about ecologically reasonable methods of Echinacea Pallida (Echinacea pallida (Nutt.) Nutt.) pre-sowing seed treatment. The influence of seed treatment on first year of vegetation plants root productivity is analyzed.The non-treated control is compared to the others, where seeds were treated with ph...
The results of studies of officinal plants are given. The peculiarity their biology, physiology and phytochemistry, reproduction and cultivation, use in medicine and industry was considered
The paper studies the efficiency of biocorrection of seed barley (Hordeum sativum (Lessen)) productivity with stimulants of natural origin. The presowing treatment of barley seed with the extract of Echinacea pallida at a concentration of 0.01%,-0.000001% increases the content of the main photosynthetic pigments in leaves (up to 17.5%), and provide...
In the laboratory the effect of extracts of purple coneflower (Echinacea purpurea (L.) Moench) (roots, leaves, stems and inflorescences) in concentrations of 10-0.0000000001% for germination of the pollen tobacco winged (Nicotiana alata Link. et Otto) was researched. It has been established that the stimulating effect depends on the concentration a...
Presents experimental data on the effect of seed treatment pale coneflower ( Echinacea pallida ( Nutt.) Nutt.) var. "Krasunya prairie" UHF-field on seed quality and productivity of the plants.
For the first time research of dynamics of lectins activity of pale purple coneflower (Echinacea pallida(Nutt.) Nutt) in ontogenesis. It has been established that on the first year of vegetation the highest level of phytolectins observed in herbs at the second part of vegetation (14,0–16,0 point). In the roots with rhizomes lectins were appears onl...
For the fist timethe experimental results of anti-tress and antimuagenic efect of an extract of purple coneflower (Echinacea purpurea (L.) Moench) are shown. Using the extract reduces the negatie efects of mutagens on the root meristem of barley achenes, accelerates root growth and reduces the formationof chromosomal chimaeras in the tissues
For the first time in the laboratory the effect of extracts (roots, leaves, inflorescences) pale coneflower (Echinacea pallida (Nutt.) Nutt.) at concentrations of 0.1%, 0.01% and 0.001% on the germination of barley's caryopsis in comparison with a known stimulant Zircon from purple coneflower (Echinacea purpurea (L.) Moench) was investigated. It is...
The book presents the results of the study of biology, ecology, cultivation of medicinal plants, their biological activity and phytochemistry by researchers in Ukraine and Russia.
During the studying of hemagglutination activity of Echinacea extracts depending on the extraction conditions, purifica-tion degree by low-temperature ethanol fractionating solution pH it was established that lectins evince their action to the most degree in acid zone (pH=4,0–4,4). As a result an original methodology of hemagglutination activity of...
Data which is based on long-term research of dynamics of lectins activity of purple coneflower (Echinacea purpurea (L.) Moench) in ontogenesis are represented. For the first time ascertained that on the first year of vegetation the highest level of hemagglutinins was observed in August-September. Meanwhile, their activity was high in stems (from 4,...
Dependence of sowing qualities and phytosanitory condition of seeds coneflower (Echinacea purpurea (L.) Moench.) cv. ‘Zirka Mykoly Vavylova’ and pale coneflower (Echinacea pallida (Nutt.) Nutt.) cv. ‘Krasunja of praries’ from terms of storage. The specific structure micromycetes on echinacea’s seeds and structure of the pathogenic complex was analy...
The review presents information on extractive, hydrophilic and lipophilic substances, macro- and microelements of the Echinacea purpurea (L.) Moench type already introduced into medical practice, and those that are just beginning to be studied in the CIS countries at the present time: Echinacea angustifolia DC. and Echinacea pallida (Nutt) Nutt., a...
Soil exhaustion in fields used for growing sugar beet can be determined more efficiently if sample of soil is tested for the activity of lectins, and compared with control. Tests show that the haemagglutinating activity of the lectins represents a much more reliable indicator of soil exhaustion than the known polyphenoloxidase and catalase activiti...
In the year before planting of the leguminous plants, soil samples at a depth of 0-30 cms, are collected manually from various sections of the field and the samples are dried, ground and mixed with a phosphate-citrate buffer with pH of 4.5-8.0 for incubation at 37 deg.C for 2 hrs. and then at 4 deg.C for 13 hrs. The suspension is then filtered and...
A yield of 0.5% can be obtd. with the technique, which produces an agglutination titre of 1:131072-1:524288 against a figure of 1:65536 for the known method.
Crushed Hippophae rhamnoides L. seeds are extracted for 2 hrs. at room temp. with McIlwaine buffer in physiologic saline at pH 5.0-6.0 using a raw material/extracting soln. ratio of 1:10. Afte...
The activity of lectin without a sugar is determined and the difference between the readings with an without a sugar is taken as the measure of physiological activity. Retardation of haemagglutination reaction is used to determine the activity.
Typically, addn. of glucose to the sample does not affect the activity at all, therefore the physiologica...
The procedure consists of determining certain agronomical indicators - the pH level of a salt extract from the soil and the range of its haemagglutination activity. These are compared, and a high capacity for forming root nodules is observed where the value of the first indicator falls within the range of values of the second. For example, perennia...
Activity of lectins in soil is determined more efficiently as follows. The actual or exchange acidity of infusion of soil sample is first determined, and lectins are then extd. by fractionation. The erythro-agglutination activity of the lectins is then determined in a phosphate buffer at pH equal to that of the soil infusion.
Tests show that use of...
The method is based on comparison of activity of soil enzymes in soil samples from analysed (non-rotational maize cultivation) and control (rotational maize cultivation) fields.
The samples of soil from both fields are dried, milled, sieved and analysed for activity of peroxidase, catalase and urease, using the known method. This is followed by cal...
The method comprises determn. of activity of soil enzymes invertase and catalase in samples of soil taken from analysed field and control field, calculating ratio of invertase activity to catalase activity for both samples and comparing results.
The samples of soil taken from field with non-rotational winter wheat cultivation (analysed sample) and...
The method comprises taking a sample of soil from analysed field during vegetation period of sugar beet monoculture and sample of soil from control field (rotational cultivation) and analysing both samples for activity of soil enzymes: polyphenoloxidase and catalase. This is followed by calculating the soil fatigue index (SF) using expression: (SF)...
The method comprises drying and sieving soil, placing a sample in container and pouring in phosphate-citrate buffer of pH 4.2-8.0 at ratio of soil to the soln. as 1:10. Obtd. suspension (after 2 hrs. of extraction) is filtered and obtd. soln. is tested for the activity of haemagglutination reaction.
The buffer mixt. is prepd. by mixing 0.2M soln. o...
The testing method involves placing samples of the seeds in a cassette with a tray (1), wetting and germinating. The seeds can be placed in a number of cassettes, according to the size of the sample, with the thickness of the cassettes selected equal to the upper level of the dimensions of the selected seeds and the diameter of the holes (4) in the...
A relationship between setting of parthenocarpic fruits in natural sel-f-pollination, without pollination, height, diameter and crown volume of a tree the number of leaves in the corymb, on the one hand, and setting of parthenocarpic fruits in natural cross-pollination, on the other hand, has been studied using the path coefficient method. The stu...
The method involves assessing the blossom from the instant where the buds appear on various kinds of pears based on observation of the stage and character of the hair cover of the pistil of the flowers. Knowing the peculiar properties of the covering in various sorts and taking into account forecasts of drops in temp., the gibberellin sprinkling is...
According to the proposed method, the percentage of seedless fruits is recorded, on model branches, set without pollination, in castrated and isolated flowers, Pa, and from naturally self-pollinated flowers, treated with 0.01%-gibberellin soln. Ps. The mean number of leaves is counted in the inflorescence, F, on the model branches. Tendency is part...
Parthenocarpy in pears is stimulated by depositing a soln. of active material on the generative organs.
In order to obtain in the absence of pollination, or during self-pollination, well developed commercial fruit, free from seed, the active material should be a 0.001-0.01 per cent soln. of conmaidin, a phytohemaglutinin (I) from meal ex maize stig...
Questions
Questions (4)
What is the role of lectins in the soil?
In our studies we found in the soil proteins that have agglutinating activity. Their evaluation showed that they are lectins. Like soil enzymes, they may have long time to remain active. It is known that lectins in plants and other living organisms play a great role in their vital activity. The question arises, what is their role in the soil?
I will be grateful for your opinion
We are studying the possibility of growing St. John's wort. However, there are many problems associated with obtaining seedlings, the formation of agrocenosis, the durability of the plantation and so on. It would be desirable to learn or find out opinion at experts on this medicinal plant.
I am interested in the classical and modern interpretation of these concepts by researchers of different scientific schools and methods of evaluation, if they are developed. Especially organic matter in the soil. Thanks to all!
I am interested in examples (articles) of the use of path coefficients for the analysis of experimental data obtained in the study of medicinal plants. I have a special program for calculating. If anyone uses this method, I can give for use.
Projects
Projects (3)
The Organizing committee invites members of scientific institutions, young scientists and university professors, professionals in the industry to take an active part in the conference.
Thematic areas:
- Introduction, botany, biology of medicinal plants;
- Environmental problems of cultivation and processing;
- Cultivation and processing technology;
- Post-harvest processing and technology;
- Phytochemistry of medicinal plants.
Participation in the conference: distance, without payment
To gather relevant information in all areas of drug discovery research into resourceful books that will be useful to everyone.
E-mail: egbunachukwuebuka@gmail.com
Some Books of the project:
Elsevier Series on Drug Discovery Updates (Book proposals are being accepted). Series link: https://drugdiscoveryupdate.com/
Analytical Techniques in Biosciences (ongoing)
Coronavirus Drug Discovery (In production)
Neglected Tropical Diseases and Phytochemicals in Drug Discovery (John Wiley & Sons Canada, Limited, 2020; ISBN: 1119616603, 9781119616603)
https://books.google.com.ng/books/about/Neglected_Tropical_Diseases_and_Phytoche.html?id=ZK5-xAEACAAJ&redir_esc=y
Preparation of Phytopharmaceuticals for the Management of Disorders: The Development of Nutraceuticals and Traditional Medicine (Elsevier, ISBN: 9780128202852; October, 2020)
https://www.elsevier.com/books/preparation-of-phytopharmaceuticals-for-the-management-of-disorders/egbuna/978-0-12-820284-5
Drug Development for Cancer and Diabetes: A Path To 2030 (AAP/CRC Press Taylor & Francis, in press).
https://www.routledge.com/Drug-Development-for-Cancer-and-Diabetes-A-Path-to-2030/Saravanan-Egbuna-Averal-Kannan-Elavarasi-Bahadur/p/book/9781771888608
Phytochemicals as Lead Compounds for New Drug Discovery (Elsevier, ISBN: 9780128178904)
https://www.elsevier.com/books/phytochemicals-as-lead-compounds-for-new-drug-discovery/egbuna/978-0-12-817890-4
Natural Remedies for Pest, Disease and Weed Control (Elsevier, ISBN: 9780128193051)
https://www.elsevier.com/books/natural-remedies-for-pest-disease-and-weed-control/egbuna/978-0-12-819304-4
Phytochemistry: in vitro and in silico updates (Springer Nature, ISBN: 9780128193051).
https://www.springer.com/us/book/9789811369193
Phytochemistry, Vol 1: Fundamentals, Modern Techniques, and Application (Published on the 14th Dec., 2018).
https://www.crcpress.com/Phytochemistry-Volume-1-Fundamentals-Modern-Techniques-and-Applications/Egbuna-Chinenye-Ifemeje-Chidi-Udedi-Kumar/p/book/9781771887595
Phytochemistry, Vol 2: Pharmacognosy, Nanomedicine, and Contemporary Issues (Published on the 14th Dec., 2018).
https://www.crcpress.com/Phytochemistry-Volume-2-Pharmacognosy-Nanomedicine-and-Contemporary/Egbuna-Kumar-Chinenye-Ifemeje-Vikas-Kurhekar/p/book/9781771887601
Phytochemistry, Vol 3: Marine, Industrial, and Advances (Published on the 14th Dec., 2018).
https://www.crcpress.com/Phytochemistry-Volume-3-Marine-Sources-Industrial-Applications-and-Recent/Egbuna-Chinenye-Ifemeje-Kumar-Sharif/p/book/9781771887618
I will also welcome partnerships from similar projects, departments, faculties and universities.
Please contact me through egbunachukwuebuka@gmail.com; Phone: +2347039618485
