Sergei Spiridonov

Severtsov Institute of Ecology and Evolution · The Center of Parasitology IPEE RAS

Zoo conditions are unique for reindeer, since domestic reindeer are not kept in captive facilities like cattle. In the zoo, reindeer are usually surrounded by many different animals that they would never encounter naturally. Thus, they might be infected with new helminths. Numerous petting zoos raise concerns about the safety of tactile interaction...

This work presents the results of studying the molecular characteristics of parasitic tapeworms Echinococcus canadensis. The helminths were discovered during the autopsy of a wolf (Canis lupus Linnaeus, 1758) killed by hunters in the Kirov oblast in 2021. A molecular phylogenetic study was performed by analyzing the sequence of a fragment of the fi...

Oxyurid nematodes from the hindgut of the Ethiopian endemic rodent, Stenocephalemys albipes Rüppell, 1842, were examined and found to represent a new species of the genus Syphacia Seurat, 1916, Syphacia ethiopiana sp. n. The new species is morphologically similar to the common intestinal nematode of mice, Syphacia obvelata (Rudolphi, 1802) Seurat,...

Caucasus is known as one of the few biodiversity hotspots in Europe and is characterised by rich gastropod fauna while the nematode fauna in association with gastropods has remained largely understudied. Surveys conducted in 2019 and 2021 in the North Caucasus of the Russian Federation (Stavropol Upland and western and central parts of Krasnodar Kr...

A new nematode species, Heth longquani sp. n. (Hethidae, Ransonematoidea, Rhigonematomorpha, Rhabditida) is described from the camballoid millipede, Chonecambala crassicauda from Thanh Hóa province, Viet Nam. The morphological features of this new species were studied with brightfield and scanning electron microscopies. Partial sequences of LSU rDN...

Nematomorpha (hairworms) is a phylum of parasitic ecdysozoans, best known for infecting arthropods and guiding their hosts toward water, where the parasite can complete its life cycle. Over 350 species of nematomorphs have been described, yet molecular data for the group remain scarce. The few available mitochondrial genomes of nematomorphs are enr...

The Russian Arctic supports wild sympatric ruminants and their data-deficient helminths. In this study, we: (1) collected fecal samples of wild and semiwild reindeer (Rangifer tarandus), muskoxen (Ovibos moschatus), and snow sheep (Ovis nivicola) across Palearctic North territories: Arkhangelsk Oblast (including Novaya Zemlya archipelago), Karelia...

The molecular characteristics of parasitic tapeworms of the genus Echinococcus were studied. Helminths were discovered during the autopsy of a wolf ( Canis lupus Linnaeus, 1758), hunted in the Kirov region in October of 2021. Molecular phylogenetic study was performed by analyzing the sequence of a fragment of the mitochondrial cytochrome c oxidase...

A new nematode species, Phasmarhabditis villasmundi sp. n., was isolated from land gastropods in the Villasmundo-S. Alfio Nature Reserve in Syracuse Province, Sicily. Phasmarhabditis villasmundi sp. n. is characterised by the presence of males and females in the population and is recognised mainly by a conical female tail with prominent phasmids at...

https://rdcu.be/c1D0e (full-text access to a view-only version) First stage larvae of an unknown lungworm (Protostrongylidae) were isolated in the feces of a wild reindeer (Rangifer taran-dus) from Taimyr, Russia. Larvae were 365-366 μm long and had a tail spike lacking a dorsal spine. DNA analyses using BLAST showed that nuclear sequences obtaine...

A new species of thelastomatid Leidynema bestium sp. n. is described from the hind gut lumen of the cockroaches Diploptera punctata (Eschscholtz, 1922) and Elliptorhina chopardi (Lefeuvre, 1966). The new species is characterized by a combination of morphological features: presence of lateral alae in both sexes with posterior margin of alae in femal...

(https://rdcu.be/cQcAh - read only full text version) Elaphostrongylus rangiferi is a parasitic nematode specific to reindeer (Rangifer tarandus). In the 20th century, reindeer could not be found in Leningrad oblast. However, several dozen of them were brought from Murmansk oblast and the Yamalo-Nenets Autonomous Okrug by 2020 and introduced in at...

Purpose: The present study investigates the origin of Arion vulgaris slugs in the parks of Moscow city and their parasites. Methods: Snails and slugs inhabiting green areas of Moscow city were collected in the summer season of 2020 and examined on the presence of gastropod-associated nematodes and trematodes using morphological and molecular met...

Elaphostrongylus rangiferi is a parasitic nematode specific to reindeer (Rangifer tarandus). In the XXth century, reindeer were not found in the Leningrad Region. However, by 2020, several dozen of them were brought from the Murmansk Region and the Yamalo-Nenets Autonomous Okrug and settled in at least seven private menageries. As a result of a stu...

IRAKLI YASONOVICH ELIAVA (DECEMBER 23, 1928, TBILISI - NOVEMBER 30, 2021, TBILISI) Dedicated to Professor I.Ya. Eliava, outstanding Georgian soil zoologist and parasitologist, to his life, scientific contributions, and educational activities. https://www.zin.ru/journals/parazitologiya/content/2022/prz_2022_1_6_Ryss.pdf Keywords: HISTORY OF SCIENCE...

Synoecnema hirsutum Timm, 1959 (Ungellidae, Drilonematoidea), found in the body cavity of the pheretimoid earthworm at the border of Laos and Vietnam, was re-described and illustrated. The mitochondrial genome of S. hirsutum obtained with Illumina HiSeq sequencing is the first annotated mitochondrial genome as a representative of the superfamily Dr...

Phasmarhabditis quinamensis sp. n. was recovered from several tropical terrestrial gastropod species collected in Cat Tien National Park in southern Vietnam with the snail, Sesara sp., designated as the type host. Phasmarhabditis quinamensis sp. n. is recognised by males and females with a lip region set off by the circle of six triangular low prob...

It is corrected version of the Program and Abstracts of the 14th International Nematological Symposium of the RUssian SOciety of Nematologists

The 14th International Symposium of the Russian Society of Nematologists. Program and Abstracts (English version).

The Program of the 14th RSN International Nematological Symposium, Jaroslavl, Russia, 1-6 August, 2021

Synoecnemahirsutum Timm, 1959 (Ungellidae, Drilonematoidea) found in the body cavity of the pheretimoid earthworm at the border of Laos and Vietnam was redescribed and illustrated. It was molecularly characterised for the first time. It is also the first member of the superfamily Drilonematoidea for whom the mitochondrial genome was obtained.

The genetic diversity of Syphacia nematodes (intestinal parasites of rodents) was studied in the hybrid zone of two sister species of common voles, Microtus arvalis and Microtus obscurus, in the Oka River valley, east of Moscow. Syphacia nematodes of other rodent species (Microtus rossiaemeridionalis, Alexandromys oeconomus, Sylvaemus uralensis, an...

Summary. Four strains of Steinernema feltiae were isolated from agricultural fields in Anantnag district, the hilly areas of Pir Panjal region of Jammu and Kashmir Union territory of India. Phylogenetic analysis of the Internal Transcribed Spacer rDNA sequences revealed that three isolates belong to the well defined ‘Himalayan clade’ of S. feltiae,...

Phylogenetic analysis of ITS rDNA sequences of the entomopathogenic nematode Steinernema feltiae Filipjev, 1934 (Wouts, Mráček, Gerdin and Bedding, 1982) was used to infer intraspecific genetic variability of this rhabditid nematode. Nucleotide intraspecific differences among S. feltiae isolates reached the level of 19 base pairs per ITS rDNA regio...

Severianoia pachyiuli n. sp. is described from the hind gut of Pachyiulus krivolutskyi (Diplopoda: Julidae) from the Republic of Adygea, Russian Federation. Females of the new species can be distinguished from those of S. magna by a smaller body and egg size; S. glomericola by smaller eggs and S. dubia by the organisation of the genital tract. Fema...

Presently, the genus Heterorhabditis contains 16 valid entomopathogenic nematode species. In this study we used samples from 11 species: H. amazonensis, H. bacteriophora, H. baujardi, H. beicherriana, H. downesi, H. floridensis, H. georgiana, H. indica, H. megidis, H. noenieputensis, and H. zealandica to amplify and sequence five gene fragments: th...

Two new nematode species of Phasmarhabditis were isolated from land snails in North and West Caucasus, Russian Federation. Phasmarhabditis circassica sp. n. originated from Oxychilus sp. collected by the Nickel settlement in the Adygea Republic while P. clausiliiae sp. n. came from Clausiliidae gen. sp. in the Stavropol district. Phasmarhabditis ci...

The supralittoral amphipod Traskorchestia ditmari (Derzhavin, 1923) was identified as the intermediate host for Antechiniella septentrionalis Ivanova, Dokuchaev & Spiridonov, 2019, a parasite of the tundra vole Microtus oeconomus and Skrjabinocerca sp. (both Spirurida: Acuariidae) in Magadan Oblast in north-eastern Russia. Joint infection by both l...

The intestinal parasitic nematode, Baylisascaris transfuga, was recorded in wild rodents for the first time. Representatives of four murid species (15 Myodes rufocanus, 10 M. rutilus, 3 M. glareolus and 27 Microtus oeconomus) were collected in the White Sea coastal habitats in the south of the Kola Peninsula, Russia in July 2015 and examined for pa...

Inverted repeats are common DNA elements, but they rarely overlap with protein-coding sequences due to the ensuing conflict with the structure and function of the encoded protein. We discovered numerous perfect inverted repeats of considerable length (up to 284 bp) embedded within the protein-coding genes in mitochondrial genomes of four Nematomor-...

The study of parasites in recently established populations of invasive species can shed light on the sources of invasion and possible indirect interactions between the alien species and native ones. We studied parasites of the global invader Harmonia axyridis (Coleoptera: Coccinellidae) in the Caucasus. In 2012, the first established population of...

Statistical analysis of data on the infestation of males and females of Harmonia axyridis with Hesperomyces virescens and Parasitylenchus bifurcatus in the Caucasus. (DOC)

Localities of Harmonia axyridis and its parasites Hesperomyces virescens and Parasitylenchus bifurcatus in the Caucasus. (XLS)

Raw morphometric data on Parasitylenchus bifurcatus. (DOCX)

Study of parasites in recently established populations of invasive species can shed lite on sources of invasion and possible indirect interactions of the alien species with native ones. We studied parasites of the global invader Harmonia axyridis (Coleoptera: Coccinellidae) in the Caucasus. In 2012 the first established population of H. axyridis wa...

Antechiniella septentrionalis n. sp. (Spirurida: Acuariidae) is described from the duodenum of a tundra vole, Microtus oeconomus (Pallas), collected in the Magadan region in the north-east part of Russia. It differs from A. suffodiax (Beveridge & Barker, 1975) and A. sertatum Smales, 1991 mainly in having a larger number of postcloacal papillae (5–...

Brief review of presentations in the English language Nematology and Ecdysozoa Symposium of the Russian Society of Nematologists.

A new nematode species recovered from the laboratory culture of Drosophila melanogaster is described and illustrated. The mass reproduction of Panagrellus levitatus sp. n. in the fly culture occurred several times and resulted in a significant reduction of the fly population. Nematode outbreaks happened after the introduction of D. melanogaster to...

First results of the parasitological survey of Vietnamese terrestrial invertebrates performed in 2007 demonstrate extreme diversity of nematodes parasitic in these animals. The most interesting new findings are nematodes of four superfamilies: Drilonematoidea, Thelastomatoidea, Rhabditoidea and Ransomnematoidea. Further progress was achieved in las...

The oral nematode infection has been identified from a private collection of Theraphosidae spiders. The disease has latent and explicit stages. The mechanisms of occurrence and course of nematodosis are considered. Recommendations on ways to prevent the spread of infection and way to cure are presented.

The muscular tissue samples were collected from the corpses of several leopard cats found in autumn-spring period on the territory of the Sikhote-Alin nature reserve. These samples were transported to All-Russian Institute for Fundamental and Applied Parasitology of Animals and Plants. Muscle tissue biopsy was used to detect Trichinella juveniles....

The nematodes of the genus Baylisascaris are common intestinal parasites of sables (Martes (M.) zibellina kamtschadalica Birula, 1916) on the entire territory of Kamchatka peninsula. Partial sequences of Cox I mitochondrial gene were used for molecular characterization of these nematodes, which confirmed the identification based on morphological da...

Partial nucleotide sequences of the two mitochondrial genes (cytB and COI) were obtained for 13 Trichinella isolates from different regions of the Russian Federation. All the cytB mtDNA sequences were identical with the sequences of T. nativa from Canada, showing no nucleotide differences between isolates. Analysis of partial COI mtDNA sequence con...

The informativity of some regions of the ribosomal cluster (rDNA) in freshwater horsehair worms (Nematomorpha) has been analyzed to trace the nucleotide differences for discrimination of species and genera of these parasites using available sequences from the NCBI GenBank and our data.

Redescription of Steinernema (Neoaplectana) thesami Gorgadze, 1988 was done based on the nematode strain isolated in Georgia in 1987 from an infected lepidopteran, Operophtera brumata, and since then maintained under laboratory conditions. Molecular analysis has shown the similarity of the ITS rDNA sequence of S. thesami with that of recently descr...

Acanthocephalans Prosthenorchis cf. elegans were found in primates in the Moscow Zoo. The larvae of these parasites (cistacanths) were found in cockroaches Blattella germanica that had been captured near aviaries of infected animals. Descriptions and drawings of adult parasites and their larvae are given. Analysis of Prosthenorchis cf. elegans gene...

A new nematode genus and species associated with terrestrial gastropods from Vietnam is described and illustrated. Partial SSU and ITS rDNA sequences were obtained and subjected to phylogenetic analysis. Both morphologically and molecularly, Neoalloionema tricaudatum gen. n., sp. n. is closest to Alloionema appendiculatum, another gastropod associa...

Three new species of Filiponema, Filiponemasp., and a new species of Iponemaare described from earthworms. Filiponema champasp. n., from the coelomic cavity of Pheretimasp. from Vietnam, is closest to F. cylindropharyngatumand F. javanicumbut differs in having a wider body, longer spicules and more conspicuous excretory pore. Filiponema yaoensesp....

A new species of the genus Synoecnema-S. watinagii sp. n.-is described and illustrated. The species is characterized by its small size, lack of sexual dimorphism apart from sexual characters, males with pericloacal disk, females with anterior vulva position and lacking an anus, and similar caudal organs in both sexes in the shape of long, deep slit...

The prevalence of toxocariasis was studied among a population of domestic and stray dogs and cats. In a megalopolis, Toxocara invasion was 31.6% in a population of cats and 82 and 25.4% in that of puppies and dogs, respectively. A method was developed for the molecular genetic identification of T. cati and T. canis.

The cuticular structure of the horsehair worm Gordionus alpestris (Villot, 1885) was studied under scanning and transmission electron microscopes. Adult worms were collected in the Syuk River near Nickel' Village in the Republic of Adygea (Russia) in June 2013. In the sampling area, the G. alpestris juveniles parasitize diplopods Pachyiulus krivolu...

Epizootological surveys on the Arctic coasts of the Russian Federation revealed 8 terrestrial andmarine mammal species that were Trichinella carriers. The infection rate varied with the animal species from 1.6 to 92.8%. Analysis of the taxonomic affiliation of Trichinella isolated from the muscles of the terrestrial and marine mammals indicated tha...

The results of testing several primer combinations were used to choose an optimal pair for the amplification of the internal transcribed spacer 2 (ITS2) region of ribosomal DNA (direct: Tri58s F 5 CGG TGG ATC RCT TGG CTC GTA CG and reverse: AB28 Rr (CGA CCG CTT ATT GAT ATG C). This pair of primers yields a 900 bp PCR product. Comparative analysis o...

The nematode Heth impalutiensis n. sp. is described from an unidentified spirostreptid millipede (Harpagophoridae) from the Bukidnon Province of Mindanao, the Philippines. Based on morphological characters, H. impalutiensis n. sp. is closest to Asian-Pacific representatives of the genus. Females of H. impalutiensis n. sp. are close to H. dimorphum...

The nematode Carnoya philippinensis sp. n. is described from spirobolid millipedes (Diplopoda: Spirobolida: Rhinocricidae) from Mindanao Island, Philippines. The presence of a broad cuticular annulation and a long subulate tail in both sexes make C. philippinensis sp. n. closest to Australasian and Pacific representatives of the genus. The new spec...

ON THE DIVERSITY OF NEMATODES PARASITIC IN INVERTEBRATES OF MINDANAO ISLAND, PHILIPPINES. Ivanova1, E.S., N.H.N. Sumaya2, A.B. Mohagan3 and S.E. Spiridonov1.1A.N. Severtsov Institute of Ecology and Evolution, Russian Academy of Sciences, Leninskii pr., 33, Moscow, 119071, Russia; 2Biology Department, Mindanao State University-Iligan Institute of te...

Drasico n. g. is erected to accommodate two new species of nematode, Drasico nemoralis n. sp. and D. paludigenus n. sp., recovered from coelomic cavities of Drawida ghilarovi Gates, endemic earthworms of the Russian Far East. The new genus is characterised by the following unique for the Synoecneminae characters: apical portion of the head attenuat...

An isolate of Steinernema cameroonense Ngo Kanga et al., 2012, which differs from type one in 2 bp of the ITS rDNA region, has been cultured on Galleria mellonella since 2011. The body length and pharynx length of the infective juveniles of this isolate differ from those reported for the type isolate (ranges do not overlap). The taxonomically impor...

SUMMARY In Russia, both alveolar and cystic echinococcoses are endemic. This study aimed to identify the aetiological agents of the diseases and to investigate the distribution of each Echinococcus species in Russia. A total of 75 Echinococcus specimens were collected from 14 host species from 2010 to 2012. Based on the mitochondrial DNA sequences,...

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A new isolate of Steinernema costaricense Uribe-Lorío, Mora & Stock, 2007 (Panagrolaimorpha: Steinernematidae) was discovered from the Bush Augusta State Park, Missouri, USA, and its morphological and molecular examination was carried out. Morphologically, adults of the Missouri isolate are very close to S. costaricense from Costa Rica. Infective j...

The present invention relates to a nucleic acid sequences from Xenorhabdus that encodes a GroEL protein. The invention encompasses nucleic acid molecules present in non-coding regions as well as nucleic acid molecules that encode proteins, fragments of proteins, tRNA's, fragments of tRNA's, rRNA's and fragments of rRNA's. In addition, protein and f...

Specimens sampled in Central Mongolia have been examined for the intraspecific polymorphism of the nucleotide sequences of ribosomal DNA internal transcribed spacers (ITS1 + 5.8S + ITS2) of the parasitic nematode Haemonchus contortus. A considerable diversity of haplotypes differing in the nucleotide composition of this DNA region has been observed...

During the survey of Pachyiulus krivolutskyi Golovatch, 1977 (Diplopoda) near Nickel’ in Adygei Republic (Russia) in the summer 2009 the infestation of these millipeds with nematomorphs of the species Gordionus alpestris was revealed. The morphology of naturally emerging nematomorphs was studied in SEM. The partial sequences of 18S (883 bp) and 28S...

Short surveys on the nematode fauna of terrestrial molluscs in Sofia, Bulgaria and Crimea, Ukraine were performed. The composition of nematode fauna was compared with data from the literature and in our records of nematode fauna from different regions of continental Europe and the British isles. Two nematode species, Angiostoma dentiferum Mengert,...

A new Alaninema species associated with leaf-veined slugs (Athoracophoridae) in New Zealand is described and illustrated. Alaninema ngata n. sp. is characterised by a very long, cylindrical body, rounded head bearing two circles of cephalic papillae and amphids, shallow stoma with three bifid teeth, muscular, cylindroid pharynx lacking valves, enla...

Ichthyocephaloides sumbatus n. sp., from the gut of Salpidobolus sp. (Spirobolida: Rhinocricidae), collected in the forest covering the mountainous region of Sumba Island, Indonesia, is described and illustrated. The new species is morphologically related to Australasian species of Ichthyocephaloides by the presence of a long, subulate tail in both...

Two species of the genus Pseudonymus Diesing, 1857, P. spirotheca (Györy, 1856) and P. islamabadi (Basir, 1941), were obtained from the hindgut of water scavenger beetles (family Hydrophilidae), caught in the estuary of the River Volga on the territory of the Astrakhan State Biosphere Reserve (June, 2012). For both species, new morphological charac...

The egg-shell ultrastructure of Blatticola blattae has been studied under scanning and transmission electron microscopy. Similar to other studied oxyurids, it consists of five layers: a lipid layer, a chitinous layer, a vitelline layer, internal and external uterine layers. Unlike in the closely related thelastomatid Hammerschmidtiella diesingi, th...

Travassosinema dalei sp. n. (Thelastomatidae, Oxyuromorpha, Rhabditida), a new species of pinworms, is described from the posterior gut of a scarabaeid larva collected at Mount Glorious, Queensland, Australia. Numerous males of T. dalei sp. n. were discovered together with females bearing cephalic umbraculum, a set of six cephalic wings, which is c...

##Assembly-Data-START## Sequencing Technology :: Sanger dideoxy sequencing ##Assembly-Data-END##

Two new species of Siconema (Drilonematoidea: Ungellidae) from Pu Mat Nature Reserve, Vietnam are described and illustrated. Siconema ovicallosum sp. n. is characterised by a wide female body, female caudal organs displaced to posterior extremity followed by short tail tip; males short, slim with caudal organs near cloacal opening and long, conical...

Steinernema cameroonense sp. n. and S. nyetense sp. n. are two new species isolated from soil samples in Minkama, Obala and Nkontangan, Nyété, respectively. Steinernema cameroonense sp. n. is characterised by infective juvenile body length averaging 622 (490-693) μm; pharynx length of 108 (100-114) μm; tail length of 76 (52-107) μm; D and E values...

Additonal morphological data are provided for Steinernema jollieti Spiridonov, Krasomil-Osterfeld, Moens, 2004. A light and scanning electron microscopy were used to particularize taxonomically important characters missing in the first decsription. The GS% and SW% indices are provided for males. The distribution of genital papillae on male tail is...

Insulanema longispiculum, a new genus and species of Carnoyidae (Rhigonematomorpha), is described from the hind gut of diplopod Apeuthes Attems, 1937 (Spirobolida: Trigoniulidae), collected on Phu Quoc Island in the Kien Giang Province, Vietnam. The new genus is characterised by the absence of any spines or other cuticular formations in the cervica...

A new species of entomopathogenic nematode, Steinernema lamjungense n. sp., was recovered from soil samples collected from Tarku, Lamjung district of Nepal, during a survey in 2007. The analysis of ITS-rDNA sequence placed S. lamjungense n. sp. in the arenarium-glaseri-karii-longicaudum group. The nematode can be separated from other described spec...