Serge Cosnier

Serge Cosnier
Université Grenoble Alpes

PhD

About

450
Publications
45,219
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19,471
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Introduction
Dr Serge Cosnier is Research Director exceptional class at CNRS at the Grenoble university (France). He is Member of the Academia Europaea, Member of the European Academy of Sciences and Fellow of the American Institute for Medical and Biological Engineering His activity is focused on biosensors, conducting polymers, modified electrodes and biofuel cells. Dr Cosnier has authored over 376 publications (h-index 65), 3 books and 20 book chapters and holds 20 patents.

Publications

Publications (450)
Article
Full-text available
A new concept of hollow electrode based on the assembly of two buckypapers creating a microcavity which contains a biocatalyst is described. To illustrate this innovative concept, hollow bioelectrodes containing 0.16–4 mg bilirubin oxidase in a microcavity were fabricated and applied to electroenzymatic reduction of O2 in aqueous solution. For hemi...
Article
For four decades, the functionalization of electrodes by biomaterials based on electrogenerated polymers, carbon nanotubes and / or nano-objects, was widely used in the field of analytical chemistry and energy conversion. Recently, we redesigned enzymatic bioelectrodes in order to produce original objects. An innovative concept of reagentless biose...
Article
This review article focusses on new advances in the field of enzyme fuel cells (EFCs), especially, on flexible materials which can be used to make flexible EFCs for wearable devices, three-dimensional (3D) printed structures to prepare electrodes for EFCs and micro/nano electromechanical structures (MEMS/NEMS) to fabricate micro-EFCs. Particular at...
Article
Phage is a human-safe bacteria-infecting virus assembled from proteins and nucleic acids, enabling it to be genetically engineered to display functional peptides or proteins. It is also a biological nanomaterial that can be assembled into ordered nanostructured materials, laying the foundation for advancing nanobiotechnology. Phage, alone or in com...
Article
Rational design highly active transition metal or transition metal oxides/nanocarbon hybrids is one of the feasible way to deliver a cooperative oxygen reduction (ORR) with a synergistic effect of the hybrid interface. Herein, based on benzimidazole-5-carboxylate (BIMC), CoO/Co-N-C nanocomposites are fabricated via 2-methylimidazole (2-MeIm)-tuned...
Article
We report the synthesis of coated gold nanoparticles with nitrobenzoic moieties (AuNPs-TNB). They displayed small and narrow size distribution (2 nm) as shown by TEM imaging. These functionalized AuNPs-TNB were physically adsorbed on multi-walled carbon nanotubes (MWCNTs) electrodes. Their stable adsorption was monitored by electrochemical methods....
Article
Glyconanoparticles (GNPs) made by self-assembly of carbohydrate-based polystyrene-block-β-cyclodextrin copolymer are used as a building block for the design of nanostructured biomaterials of electrode. The firm immobilization of GNPs is carried out on electrochemically generated polymer, poly(pyrrole-adamantane), and copolymer, poly(pyrrole-adamant...
Article
Reasonable control of the redox states within the catalytic units together with the interconnection degrees of the substrate is of great significance in the modulation of a well-performing transducer. Herein, a novel carbon black (CB)-modified copper metal-organic framework nanomaterial (CB@Cu-MOF) prepared at room temperature was utilized as a pre...
Article
Full-text available
This work demonstrates the first example of sweat‐based wearable and stretchable biosupercapacitors (BSCs), capable of generating high‐power pulses from human activity. The all‐printed, dual‐functional, conformal BSC platform can harvest and store energy from sweat lactate. By integrating energy harvesting and storage functionalities on the same fo...
Article
A series of polycyclic aromatics, naphthalene, phenanthrene, perylene, pyrene, 1-pyrenebutyric acid N-hydroxysuccinimide ester (pyrene NHS) and coronene, were immobilized via π stacking on carbon nanotube (CNT) electrodes and electro-oxidized in...
Article
Biosensors are essential tools in the health and the environmental sectors since they allow fast and reliable diagnostics and analysis. Biosensors are defined by the biological sensing element, which contains biomolecules or synthetic, bioinspired entities with unique specificity towards the analyte. The biological or bioinspired recognition even c...
Article
Metal-organic frameworks (MOFs) act as versatile coordinators for the subsequent synthesis of high-performance catalysts by providing dispersed metal-ion distribution, initial coordination condition, dopant atom ratios, and so on. In this work, a crystalline MOF trans-[Cu(NO3)2(Him)4] was synthesized as the novel precursor of a redox-alternating Cu...
Article
The non-covalent modification of carbon nanotube electrodes with pyrene derivatives is a versatile approach to enhance the electrical wiring of enzymes for biosensors and biofuel cells. We report here a comparative study of five pyrene derivatives adsorbed at multi-walled carbon nanotube electrodes to shed light on their ability to promote direct e...
Article
Full-text available
A series of new glyconanoparticles (GNPs) was obtained by self-assembly by direct nanoprecipitation of a mixture of two carbohydrate amphiphilic copolymers consisting of polystyrene-block-β-cyclodextrin and polystyrene-block-maltoheptaose with different mass ratios, respectively 0–100, 10–90, 50–50 and 0–100%. Characterizations for all these GNPs w...
Article
Enzyme mimetics have attracted wide interest due to their inherent enzyme-like activity and unique physicochemical properties, as well as promising applications in disease diagnosis, treatment and monitoring. Inspired by the attributes of nonheme iron enzymes, synthetic models were designed to mimic their capability and investigate the catalytic me...
Article
A biosensor based on the release of the enzyme substrate from its structure was developed for the inhibitive detection of benzoic acid. A polyurethane support comprising two perforated microcapsules (800 μm in diameter) filled with methylene blue as a model compound and covered with a conductive deposit of multiwalled carbon nanotubes, continuously...
Article
Full-text available
The fabrication of bioelectrocatalytic protein electrodes by the simple compression of carbon nanotube and protein powders was investigated using a series of proteins including bilirubin oxidase (BOx), bovine serum albumin (BSA), catalase, cytochrome C (Cyt C), diaphorase, FAD-dependent glucose dehydrogenase (FAD-GDH), galactose oxidase (GAOx), glu...
Article
In traditional coreactant electrochemiluminescence (ECL), the efficiency of the coreactant catalyzed into an active intermediate is one of the dominant factors restricting the luminous intensity. In this work, Co-2-MI-ZnTCPP is designed as a composite material integrating coreaction accelerator (Co–N) and luminophore. Through the catalytic effect o...
Article
The Cover Feature depicts the covalent modification of multiwalled carbon nanotubes (MWCNTs) with Azure A groups via diazonium chemistry for the electrical wiring of FAD‐dependent glucose dehydrogenase for catalytic glucose oxidation. Superior mediated bioelectrocataylsis is observed using the covalent rather than classical adsorption approach. Mor...
Article
This work focuses on the use of electrically contacted carbon nanotube forests as an electrode material for the bioelectrocatalytic reduction of oxygen to water. The forests are directly grown by chemical vapor deposition on a conductive tantalum layer, which provides enough mechanic stability during several functionalization and enzyme immobilizat...
Article
The electrochemical reduction of aryldiazonium salts is a versatile and direct route to obtain robust covalently‐modified electrodes. We report here a comparative study of Azure‐A modified carbon nanotube electrodes prepared by diazonium electrografting and by physical adsorption for bioelectrocatalytic glucose oxidation with fungal FAD‐glucose deh...
Article
Full-text available
A homemade gold electrode is modified with a carbon nanotubes/gold nanoparticles nanocomposite to perform selective and sensitive electrochemical detection of dengue toxin. This nanostructured composite offers a large specific surface and a reactive interface allowing the immobilization of biological material. Dengue antibodies are immobilized on g...
Article
Rational design and facile synthesis of highly active electrocatalysts with low cost for oxygen reduction reaction (ORR) are always of great challenge. Specifically, development of a new type of energy-saving materials with convenient method is regarded as the current bottleneck. Herein, an innovative strategy based on amino trimethylene phosphonic...
Article
Precise control over composition, morphology and size of porphyrin-based metal-organic frameworks is challenging but the extension of these hybrid materials will enable the creation of novel electrochemiluminescence (ECL) emitters. The coordination of various entities are made from Zn2+ ions and meso-tetra(4-carboxyphenyl)porphine (TCPP), modulated...
Article
WS2 nanotubes functionalized with carboxylic acid functions (WS2-COOH) were used for improved immobilization of the enzyme tyrosinase in order to form an electrochemical biosensor towards catechol and dopamine. The nanotubes were deposited on glassy carbon electrodes using a dispersion-filtration-transfer procedure to assure the reproducibility of...
Article
Full-text available
This work demonstrates a stretchable and flexible lactate/O2 biofuel cell (BFC) using buckypaper (BP) composed of multi‐walled carbon nanotubes as the electrode material. Free‐standing BP, functionalized with a pyrene‐polynorbornene homopolymer, is fabricated as the immobilization matrix for lactate oxidase (LOx) at the anode and bilirubin oxidase...
Article
A particularly efficient redox mediator for electron transfer between FAD‐dependent glucose dehydrogenase (FAD‐GDH) and carbon nanotube (CNT) based electrodes can be obtained via electrosynthetic oxidation of pyrene in aqueous buffer solution. 1H‐NMR spectroscopic studies reveal the formation of a 2 : 1 mixture of 1,6‐pyrenedione and 1,8‐pyrenedion...
Article
Tuning the coordination environment between metal centers and organic ligands is a promising way to design of the precursors of electrocatalysts for water splitting or other renewable energy options. In this work, a novel coordinated polymer was prepared feasibly in polyvinyl pyrrolidone (PVP) aqueous solution using aminotrimethylene phosphonic aci...
Article
Glycopolymers have emerged as powerful and versatile glycan analogs for the investigation of cellular signal transduction. In this study, a layer of glycopolymer-brush (GlyB) interface was functionalized on the surface of gold substrates. In order to enhance the capability and accessibility of this transducer interface, a combined protocol of coppe...
Article
Reliable and sensitive detection of xanthine has important medical and biological significance. In this work, a novel three-dimensional (3D) conductive polymer hydrogel of polyaniline (PAni) was feasibly prepared using aniline (Ani), amino trimethylene phosphonic acid (ATMP) and ammonium persulfate ((NH4)2S2O8) as monomer, gelatinizing agent and ox...
Article
A dinuclear copper complex mimicking the active site of copper oxidase was designed and π-stacked on a carbon nanotube (CNT) electrode thanks to pendent pyrene groups. The high oxygen reduction reaction activity of such CNT-supported dicopper complexes in pH 4 electrolyte allowed the construction of a Pt-free hydrogen/air fuel cell using bioinspire...
Article
Full-text available
A novel water‐soluble Ru complex bearing phenanthrolinequinone ligands, diethyleneglycol linkers and pyrene anchoring groups was synthesized. Electrical wiring of NAD‐ and FAD‐dehydrogenases is demonstrated via NADH oxidation and mediated electron transfer respectively. Bioelectrocatalysis is facilitated by the flexible and hydrophilic PEG groups,...
Patent
The invention concerns a bioelectrode and a device comprising same, for detecting or oxidising glucose. The bioelectrode of the invention comprises a layer of carbon nanotubes to which aromatic molecules are bonded, and FAD-GDH enzymes being adsorbed on the aromatic molecules. The invention applies to the field of biosensors and biofuel cells in pa...
Article
Porphyrin based nanomaterials have attracted widespread attention due to their similarity of the key dyes of natural photosynthesis. To develop new chromophores for photoelectrochemical (PEC) devices, in this work, we synthesized 3 different aggregated nanostructures of meso-tetra(4-carboxyphenyl)porphine (TCPP) via self-assembly technique. The mic...
Chapter
The possibility to harvest energy out of nontoxic and noncombustible compounds such as sugar opened the way for glucose biofuel cells, a research field with steady growing interest. Biological catalysts, enzymes, have the capacity to efficiently oxidize sugars and, in particular, glucose, mostly in a two‐electron oxidation process, at neutral pH an...
Article
We describe the fabrication of free-standing buckypaper bioelectrodes (BP) by co-immobilization of a FAD-dependent dehydrogenase and a thionine redox partner using either a pyreneNHS linker or a bifunctional triblock polynorbornene copolymer with pendant pyrene and NHS groups. While exhibiting excellent flexibility and conductivity, the BP bioelect...
Article
Enzymatic biofuel cells are emerging technologies which gain a lot of interest as a power source for implantable devices. Effective enzyme wiring and simplification of the cell architecture are the main limitations of the fuel cell development nowadays. In the present study, spray coating was used as a reliable process to produce flexible biocathod...
Data
Full details of reagents, materials, and experimental methods, further optical, electrochemical, and electronic characterization, results from control tests, as well as calculations comparing systems with immobilized versus solubilized biocatalysts.
Article
Full-text available
Enzymatic biofuel cells are eco-friendly power sources that can deliver µW-mW outputs from renewable substrates, but their stability is a major issue owing to enzyme fragility. The vast majority of reported biofuel cells can only generate power continuously for relatively short periods of time. Here we report a novel “solubilized enzymatic fuel cel...
Article
In this work, POXC, a laccase from Pleurotus ostreatus was immobilized on carbon nanotubes (CNTs) modified by electrografting of anthraquinone and naphthoate diazonium salts. Thanks to a favorable interaction between laccase and covalently‐modified CNTs, this laccase exhibits high‐potential/high current ORR performances, surpassing the performances...
Article
A disposable ovulation test is operated using a flow stack of 5 in series connected glucose biofuel cells giving an open circuit voltage (OCV) of 3.5 V. Such tests consume at total around 60 μWh of energy and in average 1.7 mW of power during one run of around 12 min which are supplied by a CR1220 3 V lithium button battery in the commercialized de...
Article
Bioelectrocatalytic carbon nanotube pellets comprising glucose oxidase (GOx) at the anode and horseradish peroxidase (HRP) at the cathode were integrated in a glucose/H2O2 flow-through fuel cell setup. The porous bioelectrodes, separated with a cellulose membrane, were assembled in a design allowing the fuel/electrolyte flow through the entire fuel...
Patent
L'invention concerne une bioélectrode et un dispositif la comprenant, pour la détection ou l'oxydation du glucose. La bioélectrode de l'invention comprend une couche de nanotubes de carbone auxquels des molécules aromatiques sont liées, et des enzymes FAD-GDH étant adsorbées aux molécules aromatiques. L'invention trouve application dans le domaine...
Article
After a short comparison of biofuel cells based on enzymes and microorganisms, several important developments and applications of enzymatic fuel cells (EFCs) are discussed. This discussion emphasizes how to evaluate the performance of EFCs, and highlights the influence of temperature and how it must be carefully considered for practical use of EFCs...
Article
Carbon nanotubes (CNTs) have been widely exploited for the development of enzymatic biofuel cells with sufficient power densities in the μW to mW range for operating low-power bioelectronics devices from renewable substrates. Buckypaper is a randomly oriented self-supporting film of carbon nanotubes, resembling an electronic paper, with excellent p...
Article
A high performance impedimetric immunosensor for the dengue virus antibody detection is presented. The setup profits from the formation of controlled and reproducible carbon nanotube (CNT) deposits on electrodes. Their easy functionalization via electrogeneration of a polypyrrole-NHS (N-hydroxysuccinimido 11-(pyrrol-1-yl) undecanoate) film enables...
Article
In this work, the direct electrochemistry of bilirubin oxidase from Magnaporthe orizae (MoBOD) was studied on CNTs functionalized by electrografting several types of diazonium salts. The functionalization induces favorable or unfavorable orientation of MoBOD, the latter being compared to the well‐known BOD from Myrothecium verrucaria (MvBOD). On th...
Article
Buckypapers have emerged as an important material for the construction of enzyme‐based electrodes and biofuel cells for energy harvesting. In this work, commercial and lab‐made buckypapers have been compared to establish their properties for future use as advanced bioelectrodes. The physical properties of the paper‐like carbon nanotube films were c...
Article
Full-text available
Two different graphene/β-cyclodextrin (CD)-based biosensors were elaborated for dopamine (DA) determination starting from glassy carbon electrode (GCE). First one was obtained by modifying GCE with reduced graphene oxide (RGO), CD and tyrosinase (Tyr) immobilized with a polyethylenimine film. The second biosensor was obtained after the incorporatio...
Patent
Full-text available
biopile à rendement amélioré
Article
We demonstrate the self-assembly, characterization and bioelectrocatalysis of redox-active cyclodextrin-coated nanoparticles. The nanoparticles with host-guest functionality are easy to assemble and permit entrapment of hydrophobic redox molecules in aqueous solution. Bis-pyrene-ABTS encapsulated nanoparticles were investigated electrochemically an...
Article
Full-text available
We report a simple, versatile, and rapid method for the fabrication of optically-transparent large-area carbon nanotube (CNT) films via flotation assembly. After solvent-induced assembly, floating films were transferred to a flat supporting substrate to form conductive and transparent CNT film electrodes. The resulting electrodes, with uniform 40 ±...
Article
Bioelectrocatalytic carbon nanotube based pellets comprising redox enzymes were directly integrated in a newly-conceived flow-through fuel cell. Porous electrodes and a separating cellulose membrane were housed in a glucose/oxygen biofuel cell design with inlets and outlets allowing the flow of electrolyte through the entire fuel cell. Different fl...
Article
In this work, we synthesized one-dimensional nanostructure of zinc 5,10,15,20-tetra(4-pyridyl)-21H,23H-porphine (ZnTPyP) via a self-assembly technique. Using sodium dodecyl sulfate (SDS) as “soft template”, the self-assembled ZnTPyP (SA-ZnTPyP) had the morphology of hexagonal nanoprisms with the uniform size (diameter of 100 nm). The SA-ZnTPyP exhi...
Article
An original impedimetric immunosensor was developed based on carbon nanotube (CNT) deposits with controlled thicknesses for enhanced electroactive surface areas leading to improved sensor performances. Cholera monitoring was chosen as the model immune system for this setup. These CNT deposits were characterized using confocal laser microscopy and e...
Article
An original interfacial hybrid cross-linker molecule combined electrochemical and photochemical properties by substitution of two functional groups, pyrene, and diazirine. The first group enables anchoring by strong π-stacking interaction or by electropolymerization onto multi-walled carbon nanotubes (MWCNT). The other photoreactive group allows th...
Article
Enzymatic biofuel cells generate electrical energy from renewable sources with high selectivity and environmental benefits compared to lithium batteries and traditional fuel cells. For enzymatic fuel cells to become competitive, major improvements in electrode design are required to enhance power density, voltage output and stability. Here we have...
Article
An original versatile methodology for molecular grafting on different surfaces via the photoinduced formation of a covalent bond based on a diazirine group is reported. The synthesis and electrochemical behavior of a new diazirine derivative which acts as a molecular linking bridge bearing both a photoactivatable covalent binding group (diazirine)...
Article
Nanomaterials have become essential components for the development of biosensors since such nanosized compounds were shown to clearly increase the analytical performance. The improvements are mainly related to an increased surface area, thus providing an enhanced accessibility for the analyte, the compound to be detected, to the receptor unit, the...
Article
For the efficient surface plasmon resonance (SPR)-based DNA assay researching, signal amplification tactics were absolutely necessary. In this work, a sensitive SPR-DNA sensor was developed by employing in situ synthesis of copper nanoparticles (CuNPs) templated by poly-T sequences DNA from terminal deoxynucleotidyl transferase (TdT)-mediated exten...
Patent
L'invention concerne une pile à biocombustible ainsi qu'un assemblage comprenant au moins deux piles à biocombustible selon l'invention. La pile à biocombustible de l'invention est destinée à être immergée dans un milieu liquide L contenant un biocombustible et un oxydant et comprend : - une première électrode choisie parmi une anode A constituée d...