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Publications (22)
The nutritional value of sheep’s milk and its derivatives is influenced by the lipid fraction, which is affected by diet and genetics. This study aimed to explore the genetic variations in the DGAT1 and SCD genes and assessed the impact of the DGAT1 genotype on milk quality in Valle del Belìce sheep, considering diet supplementation with carob pulp...
Analysis of genomic data is becoming more and more common for the
effective management of livestock breeding programmes, even in the case of
local populations. In this work, the genome-wide
data of Nero Siciliano pig
breed were compared to that of wild boar, Italian local and cosmopolitan
breeds to investigate its genetic structure, and runs of hom...
A total of twenty-seven Rossa Mediterranea lactating goats, consisting of nine homozygous for strong alleles (AA), twelve heterozygous (AF) and six homozygous for weak alleles (FF) at the CSN1S1 locus, were used to evaluate the effect of genotype, diet and genotype × diet interaction on goat milk traits and casein profile. The goats were used in a...
The current study examined the effect on milk traits and composition of the replacement of barley grain with carob pulp as a supplement for grazing ewes with different genotypes at the β-lactoglobulin (BLG) locus. Forty-six Valle del Belìce lactating ewes were chosen for the feeding trial and split into control and carob groups based on the BLG p.T...
Maedi-visna (MV) is a disease caused by small ruminant lentiviruses. It is included in the list of notifiable terrestrial animal diseases due to economic losses and animal welfare harm in the sheep sector. To date, control programs remain the onliest approach to avoiding infection. The allelic variant p.Glu35Lys (E35K) of the TMEM154 gene has been...
Purosangue Orientale Siciliano, Sanfratellano and Siciliano represent the Sicilian
equine genetic resource. This study aimed to investigate the genetic diversity,
population structure and the pattern of autozygosity of Sicilian horse populations
using genome-wide
single-nucleotide
polymorphism (SNP) data generated
with the Illumina Equine SNP70 arr...
The aim of this preliminary study was to investigate the presence of Mycoplasma agalactiae (Ma) or other Contagious Agalactia (CA) causative organisms, in hard ticks infesting milking sheep and goats in endemic areas for CA in Sicily (South-Italy). Although there is accumulating evidence to support the role of ticks in the transmission of blood-bor...
The interaction between genetic polymorphism and feeding system on milk traits and fatty acid composition was investigated in Modicana cows. Two DGAT1 K232A genotypes (AK and AA) and two feeding regimes, extensive system (EX) with 8 h of grazing without concentrate (EX) and semi-intensive systems (SI) with 2 h of grazing with concentrate, were inve...
Nanostructured electrodes detecting bacteria or viruses through DNA hybridization represent a promising method, which may be useful in on-field applications where PCR-based methods are very expensive, time-consuming, and require trained personnel. Indeed, electrochemical sensors combine disposability, fast response, high sensitivity, and portabilit...
Growth hormone participates in the regulation of lactation and lipid metabolism. A trial study was conducted to evaluate the effects of genetic polymorphism at GH p.L127V and its interaction with feeding system (extensive, EX; semi-intensive, SI) on milk traits and fatty acids composition in Modicana cows. In the semi-intensive farm (SI) diet consi...
A proliferative cauliflower lesion was excised from the udder of a sheep. Histological investigation confirmed the macroscopic classification of the lesion as a papilloma, without any fibroblastic proliferation. PCR revealed the presence of bovine papillomavirus (BPV), which was further confirmed by the identification of a Deltapapillomavirus 4 by...
Contagious agalactia (CA), an infectious disease of small ruminants, caused by Mycoplasma agalactiae, is responsible for severe losses to dairy sheep production with substantial socioeconomic impacts on small-scale farmers. The diagnosis of CA is still problematic, time-consuming and requires well-equipped labs for confirmation of outbreaks. Theref...
Mycoplasma diseases of livestock such as contagious bovine pleuropneumonia, contagious caprine pleuropneumonia and contagious agalactia still represent major problems for animal health authorities worldwide. Many significant improvements have been seen as a result of research into these degenerate bacteria, mainly in the area of diagnosis, with the...
Selection, drift, gene flow and breeding have extensively
shaped the genomic variability of domestic animals. In goat species, several mutations identified within the casein genes have been shown to
affect the level of gene expression of milk production traits. The four
casein genes – CSN1S1, CSN2, CSN1S2 and CSN3 – are organized in a cluster of
25...
Contagious agalactia (CA), a disease caused by Mycoplasma agalactiae and other pathogenic mycoplasmas, is a well-known multietiological syndrome affecting dairy breeds of sheep and goats in the Mediterranean basin. The aim of this work was to study the effect on milk production and composition of mastitis caused by M. agalactiae in Valle del Belice...
In the last years, donkey milk had evidenced a renewed interest as a potential functional food and a breast milk substitute. In this light, the study of the protein composition assumes an important role. In particular, β-lactoglobulin (β-LG), which is considered as one of the main allergenic milk protein, in donkey species consists of two molecular...
Ca, P and Mg content and distribution between soluble and colloidal phases of donkey milk (DM) were investigated in 62 individual milk samples collected from 9 Ragusano donkeys followed from the second to the ninth month of lactation. Ca (69%) and P (64%) were mainly associated with casein, while Mg was largely found in the soluble form (69%). Only...
Questions
Question (1)
Hello to every one,
I received from Bioline a Proteinase K in which protocol ("recommendation for use") should be resuspended in 50 mM Tris-HCl, 2 mM calcium acetate, pH 8. But I haven't the calcium acetate in the lab. I was wondering if I can resuspend the proteinase K using another solution.
Many thanks for your help
Serena