Serap Aksoy

Serap Aksoy
Yale University | YU · Department of Epidemiology of Microbial Diseases

About

465
Publications
42,789
Reads
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11,688
Citations
Citations since 2016
170 Research Items
5257 Citations
20162017201820192020202120220200400600800
20162017201820192020202120220200400600800
20162017201820192020202120220200400600800
20162017201820192020202120220200400600800
Additional affiliations
May 2013 - June 2013
University of Pavia
Position
  • Visiting Scientist
March 2013 - June 2013
Kenya Agricultural Research Institute
Position
  • Visiting Scientist
August 2012 - December 2012
University of Pavia
Position
  • Visiting Scientist

Publications

Publications (465)
Article
Full-text available
The ingestion of blood represents a significant burden that immediately increases water, oxidative, and thermal stress, but provides a significant nutrient source to generate resources necessary for the development of progeny. Thermal stress has been assumed to solely be a negative byproduct that has to be alleviated to prevent stress. Here, we exa...
Article
Wigglesworthia glossinidia is an obligate, maternally transmitted endosymbiont of tsetse flies. The ancient association between these two organisms accounts for many of their unique physiological adaptations. Similar to other obligate mutualists, Wigglesworthia ’s genome is dramatically reduced in size, yet it has retained the capacity to produce m...
Preprint
Lipid metabolism is critical for insect reproduction, especially for species that invest heavily into early developmental stages of their offspring. The role of symbiotic bacteria during this process is unknown but likely essential, especially in the case of obligate microbes that fulfill key biological functions in the host. Using a combined lipid...
Preprint
The ingestion of blood represents a significant pressure that immediately increases water, oxidative, and thermal stress, but provides a significant nutrient source to generate resources necessary for the development of progeny. Thermal stress has been assumed to solely be a negative byproduct that has to be alleviated to prevent stress. Here, we e...
Preprint
Full-text available
The primary vector of the trypanosome parasite causing human and animal African trypanosomiasis in Uganda is the riverine tsetse fly Glossina fuscipes fuscipes ( Gff ). We conducted a genome-wide association (GWA) analysis with field-caught Gff . To increase statistical power, we first improved the Gff genome assembly with whole genome 10X Chromium...
Article
Full-text available
Background Glossina species (tsetse flies), the sole vectors of African trypanosomes, maintained along their long evolutionary history a unique reproductive strategy, adenotrophic viviparity. Viviparity reduces their reproductive rate and, as such, imposes strong selective pressures on males for reproductive success. These species live in sub-Sahar...
Article
Full-text available
Tsetse flies ( Glossina spp.) house a population-dependent assortment of microorganisms that can include pathogenic African trypanosomes and maternally transmitted endosymbiotic bacteria, the latter of which mediate numerous aspects of their host’s metabolic, reproductive, and immune physiologies. One of these endosymbionts, Spiroplasma , was recen...
Article
Full-text available
Following publication of the original article [1], it was reported that the article copyright was incorrect. The correct copyright statement is: © This is a U.S. Government work and not under copyright protection in the US; foreign copyright protection may apply 2021. The original article [1] has been corrected.
Article
Full-text available
Tsetse flies are vectors of parasitic African trypanosomes, the etiological agents of human and animal African trypanosomoses. Current disease control methods include fly-repelling pesticides, fly trapping, and chemotherapeutic treatment of infected people and animals. Inhibiting tsetse’s ability to transmit trypanosomes by strengthening the fly’s...
Article
Full-text available
Vector control is an effective strategy for reducing vector‐borne disease transmission, but requires knowledge of vector habitat use and dispersal patterns. Our goal was to improve this knowledge for the tsetse species Glossina pallidipes, a vector of human and animal African trypanosomiasis, which are diseases that pose serious health and socioeco...
Preprint
Full-text available
Tsetse flies ( Glossina spp.) house a population-dependent assortment of microorganisms that can include pathogenic African trypanosomes and maternally transmitted endosymbiotic bacteria, the latter of which mediate numerous aspects of their host’s metabolic, reproductive, and immune physiologies. One of these endosymbionts, Spiroplasma , was recen...
Preprint
Full-text available
Tsetse flies are vectors of parasitic African trypanosomes ( Trypanosoma spp.). Current disease control methods include fly-repelling pesticides, trapping flies, and chemotherapeutic treatment of infected people. Inhibiting tsetse’s ability to transmit trypanosomes by strengthening the fly’s natural barriers can serve as an alternative approach to...
Article
Full-text available
Background The stable fly, Stomoxys calcitrans, is a major blood-feeding pest of livestock that has near worldwide distribution, causing an annual cost of over $2 billion for control and product loss in the USA alone. Control of these flies has been limited to increased sanitary management practices and insecticide application for suppressing larva...
Article
Full-text available
Glossina pallidipes is the main vector of animal African trypanosomiasis and a potential vector of human African trypanosomiasis in eastern Africa where it poses a large economic burden and public health threat. Vector control efforts have succeeded in reducing infection rates, but recent resurgence in tsetse fly population density raises concerns...
Article
Full-text available
The World Health Organization (WHO) currently classifies 20 diseases and conditions as neglected tropical diseases (NTDs). However, since its inception in 2007, PLOS Neglected Tropical Diseases has considered an expanded list that includes additional diseases with the chronic and/or debilitating, and poverty-promoting features of NTDs. Described he...
Article
Full-text available
Tsetse-transmitted African trypanosomes must develop into mammalian-infectious metacyclic cells in the fly's salivary glands (SGs) before transmission to a new host. The molecular mechanisms that underlie this developmental process, known as metacyclogen-esis, are poorly understood. Blocking the few metacyclic parasites deposited in saliva from fur...
Article
Full-text available
Background Several modeling studies have been undertaken to assess the feasibility of the WHO goal of eliminating gambiense human African trypanosomiasis (g-HAT) by 2030. However, these studies have generally overlooked the effect of vector migration on disease transmission and control. Here, we evaluated the impact of vector migration on the feasi...
Article
Full-text available
Tsetse flies (Diptera: Glossinidae) house a taxonomically diverse microbiota that includes environmentally acquired bacteria, maternally transmitted symbiotic bacteria, and pathogenic African trypanosomes. Sodalis glossinidius, which is a facultative symbiont that resides intra and extracellularly within multiple tsetse tissues, has been implicated...
Article
Full-text available
Background: Tsetse flies (Glossina sp.) are the vectors of human and animal trypanosomiasis throughout sub-Saharan Africa. Tsetse flies are distinguished from other Diptera by unique adaptations, including lactation and the birthing of live young (obligate viviparity), a vertebrate blood-specific diet by both sexes, and obligate bacterial symbiosi...
Article
Full-text available
Tsetse flies (Glossina spp.) are vectors of parasitic trypanosomes, which cause human (HAT) and animal African trypanosomiasis (AAT) in sub-Saharan Africa. In Uganda, Glos-sina fuscipes fuscipes (Gff) is the main vector of HAT, where it transmits Gambiense disease in the northwest and Rhodesiense disease in central, southeast and western regions. E...
Article
Full-text available
Tsetse flies (Glossina spp.) are vectors of parasitic trypanosomes, which cause human (HAT) and animal African trypanosomiasis (AAT) in sub-Saharan Africa. In Uganda, Glos-sina fuscipes fuscipes (Gff) is the main vector of HAT, where it transmits Gambiense disease in the northwest and Rhodesiense disease in central, southeast and western regions. E...
Article
Full-text available
Parasites elicit several physiological changes in their host to enhance transmission. Little is known about the functional association between parasitism and microbiota-provisioned resources typically dedicated to animal hosts and how these goods may be rerouted to optimize parasite development. This study is the first to identify a specific symbio...
Preprint
Full-text available
Tsetse flies (Diptera: Glossinidae) house a taxonomically diverse microbiota that includes environmentally acquired bacteria, maternally transmitted symbiotic bacteria, and pathogenic African trypanosomes. Sodalis glossinidius , which is a facultative symbiont that resides intra and extracellularly within multiple tsetse tissues, has been implicate...
Preprint
Full-text available
Background: The stable fly, Stomoxys calcitrans, is a major blood-feeding pest of livestock that has near worldwide distribution, causing an annual cost of over $2 billion for control and product loss in the United States alone. Control of these flies has been limited to increased sanitary management practices and insecticide application for suppre...
Preprint
Full-text available
Tsetse flies ( Glossina spp.) are vectors of parasitic trypanosomes, which cause human (HAT) and animal African trypanosomiasis (AAT) in sub-Saharan Africa. In Uganda, Glossina fuscipes fuscipes ( Gff ) is the main vector of HAT, where it transmits Gambiense disease in the northwest and Rhodesiense disease in central, southeast and western regions....
Article
Full-text available
Tsetse flies (Glossina spp.) vector pathogenic trypanosomes (Trypanosoma spp.) in sub-Saharan Africa. These parasites cause human and animal African trypanosomiases, which are debilitating diseases that inflict an enormous socio-economic burden on inhabitants of endemic regions. Current disease control strategies rely primarily on treating infected...
Preprint
Full-text available
Background: Tsetse flies (Glossina sp.) are the sole vectors of human and animal trypanosomiasis throughout sub-Saharan Africa. Tsetse are distinguished from other Diptera by unique adaptations, including lactation and the birthing of live young (obligate viviparity), a vertebrate blood specific diet by both sexes and obligate bacterial symbiosis....
Article
Full-text available
Understanding the mechanisms that enforce, maintain, or reverse the process of speciation is an important challenge in evolutionary biology. This study investigates the patterns of divergence and discusses the processes that form and maintain divergent lineages of the tsetse fly Glossina fuscipes fuscipes in Uganda. We sampled 251 flies from 18 sit...
Article
Full-text available
Background Symbiotic microbes represent a driving force of evolutionary innovation by conferring novel ecological traits to their hosts. Many insects are associated with microbial symbionts that contribute to their host’s nutrition, digestion, detoxification, reproduction, immune homeostasis, and defense. In addition, recent studies suggest a micro...
Article
Full-text available
Background Tsetse flies (Diptera, Glossinidae) display unique reproductive biology traits. Females reproduce through adenotrophic viviparity, nourishing the growing larva into their modified uterus until parturition. Males transfer their sperm and seminal fluid, produced by both testes and male accessory glands, in a spermatophore capsule transient...
Article
Full-text available
Background The tsetse fly (Glossina sp.) midgut is colonized by maternally transmitted and environmentally acquired bacteria. Additionally, the midgut serves as a niche in which pathogenic African trypanosomes reside within infected flies. Tsetse’s bacterial microbiota impacts many aspects of the fly’s physiology. However, little is known about the...
Preprint
Full-text available
Tsetse flies ( Glossina spp.) vector pathogenic trypanosomes ( Trypanosoma spp.) in sub-Saharan Africa. These parasites cause human and animal African trypanosomiases, which are debilitating diseases that inflict an enormous socio-economic burden on inhabitants of endemic regions. Current disease control strategies rely primarily on treating infect...
Article
The tsetse fly Glossina pallidipes, the major vector of the parasite that causes animal African trypanosomiasis in Kenya, has been subject to intense control measures with only limited success. The G. pallidipes population dynamics and dispersal patterns that underlie limited success in vector control campaigns remain unresolved, and knowledge on g...
Article
Full-text available
Background: The tsetse transmitted parasitic flagellate Trypanosoma congolense causes animal African trypanosomosis (AAT) across sub-Saharan Africa. AAT negatively impacts agricultural, economic, nutritional and subsequently, health status of the affected populace. The molecular mechanisms that underlie T. congolense's developmental program within...
Article
Full-text available
Tsetse flies (genus Glossina) are the only vector for the parasitic trypanosomes responsible for sleeping sickness and nagana across sub‐Saharan Africa. In Uganda, the tsetse fly Glossina fuscipes fuscipes is responsible for transmission of the parasite in 90% of sleeping sickness cases, and co‐occurrence of both forms of human‐infective trypanosom...
Article
Full-text available
Arthropod vectors have multiple physical and immunological barriers that impede the development and transmission of parasites to new vertebrate hosts. These barriers include the peritrophic matrix (PM), a chitinous barrier that separates the blood bolus from the midgut epithelia and thus modulates vector-microbiota interactions. In tsetse flies, a...
Data
Ultrastructure of the cardia. (A) Transversal section of a non-infected cardia. Two pictures of the same cardia were merged to produce a larger picture. B, C, D, E and F are magnified micrographs of cardia tissues. (B) Midgut tissue delimiting the outer part of the cardia. (C) Foregut tissue invagination within cardia, corresponding to the stomodea...
Data
Differentially expressed (DE) transcripts in parasitized cardia. (A) A total of 2,093 transcripts were DE in inf+/- and inf+/+ cardia relative to uninfected (non-inf) controls. In inf+/- cardia (red), 429 and 327 transcripts were up and downregulated, respectively. In inf+/+ cardia (blue), 278 and 413 transcripts were up and downregulated, respecti...
Data
Detailed results and statistics for infection experiments and oxidative stress quantification. (XLSX)
Data
GO terms analysis results. (XLSX)
Data
Parasite quantity in midgut and cardia. (A) Number of parasites in the cardia of Inf+/- (red) and Inf+/+ (blue) flies. (B) Number of parasites in the midgut of Inf+/- (red) and Inf+/+ (blue) flies. The black bar represents the mean of the replicates for each treatment. Midgut and cardia were dissected from eight 40 days-old females. Parasites were...
Data
Expression of chitin synthase after RNAi treatment. Expression of chitin synthase relative to constitutively expressed ß-tubulin after treatment with dsRNA-gfp (control; white circles) and dsRNA-chitin synthase (dsCS; gray circles). chitin synthase expression is significantly decreased after RNAi knockdown (Student t-test, p = 0.011). (TIF)
Data
Overview of cardia transcriptomes. (A) Number of RNA-seq reads in each of three biological replicates from Non-inf, Inf +/- and Inf +/+ cardia. (B) Proportion of total trimmed reads that map to Glossina morsitans morsitans or Trypanosoma brucei brucei 927. (C) Percent relative abundance of mapped Glossina morsitans morsitans transcripts. (TIF)
Data
Detailed results and analyses fo each transcriptome. (XLSX)
Data
Ultrastructure of muscles and mitochondria in cardia from inf+/- tsetse. (A) Transversal section of muscle tissues (MU) composing the sphincter. (B-D) Longitudinal section of muscles (MU) layering the midgut tissues. Micrographs in this image represent three of five of biological replicates from cardia inf+/-. Black arrowheads: healthy mitochondria...
Preprint
Full-text available
Arthropod vectors have multiple physical and immunological barriers that impede the development and transmission of parasites to new vertebrate hosts. These include the peritrophic matrix (PM), a chitinious barrier that separates the blood bolus from the midgut epithelia and modulates the vector-microbiota interactions. In tsetse flies, a sleeve-li...
Article
Full-text available
Tsetse flies are important vectors of human and animal trypanosomiasis. Ability to reduce tsetse populations is an effective means of disease control. Lactation is an essential component of tsetse’s viviparous reproductive physiology and requires a dramatic increase in the expression and synthesis of milk proteins by the milk gland organ in order t...