
Sema S HakkiUniversity of Michigan | U-M · School of Dentistry
Sema S Hakki
PhD
Prof, Selcuk University, Faculty of Dentistry
About
133
Publications
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Introduction
the effects of exosomes derived osteoblasts on the macrophages
Additional affiliations
November 1999 - June 2000
October 1995 - November 1999
May 2002 - present
Publications
Publications (133)
This case report presents the intentional periodontal maintenance of two periodontal hopeless lower central incisors with a multidisciplinary approach and 20-year follow-up. Case presentation: A 36-year-old male, in 2001, was diagnosed with aggressive periodontitis, gingival swelling, bleeding, and mandibular central incisors with mobility and poor...
Background/Objectives
It has been repeatedly demonstrated that cementum formation is a crucial step in periodontal regeneration. Hyaluronic acid (HA) is an important component of the extracellular matrix which regulates cells functions and cell–cell communication. Hyaluronic acid/derivatives have been used in regenerative periodontal therapy, but t...
The effect of preosteoblast‐derived exosomes on bone marrow macrophages (BMMΦ) and calvarial osteoblasts (cOB) was evaluated in vitro , and bone formation studies were performed in vivo in mice. Preosteoblastic MC3T3‐E1 clone 4 (MC4) cell‐derived exosomes (MC4exo) were characterized with particle tracking, transmission electron microscopy and weste...
Boric acid (BA) has been found to have therapeutic effects on periodontal disease through beneficially affecting antibacterial, anti-viral, and anti-inflammatory actions. Thus, a study was conducted to determine the effect of BA on cell viability and on mRNA expressions of proinflammatory and anti-inflammatory cytokines and on oxidative stress enzy...
Background: Stem cell-based approaches in regenerative periodontal therapy have been used in different experimental models. In this study, the effect of local application of gingival mesenchymal stem cells (GMSC) in fibroin/chitosan oligosaccharide lactate hydrogel (F/COS) on periodontal regeneration was evaluated using experimental periodontitis m...
Background:
Resolvins are endogenous mediators of the resolution of inflammation. They are derived from omega-3 polyunsaturated fatty acid precursors. Resolvin D1 (RvD1) and Resolvin E1 (RvE1) are the best-characterized members for actively promoting periodontal regeneration in experimental animal models. Here, we evaluated the efficacy of RvD1 an...
Purpose of Review
Behçet’s disease (BD) is a recurrent, chronic inflammatory disease affected by environmental triggers. Oral aphthous ulcerations are the most common and often the first sign of the disease. Although the etiology of BD has not been clearly established, its systemic effects originate from vasculitis and involve almost all organ syst...
Purpose of Review
Metabolic syndrome is a systemic condition that has components such as obesity, dyslipidemia, hyperglycemia, and hypertension and is associated with an increased risk of cardiovascular disease and type 2 diabetes. Periodontitis is a chronic inflammatory disease characterized by progressive attachment loss that develops as a result...
Purpose: To detect the effect of Resolvin D1 (RvD1) on Developmental endothelial locus-1 (Del-1) and cytokine expressions of human gingival fibroblast cells exposed to Porphyromonas gingivalis lipopolysaccharide (P. gingivalis-LPS). Material and Methods: The effect of RvD1 on cell viability of human gingival fibroblasts exposed to P. gingivalis-LPS...
Nicotine, the major bioactive ingredient in tobacco, is a major risk factor for periodontal disease and destruction. Nicotine has been shown to stimulate the production of cytokines that are priming agents for inflammation that induces tissue destruction, such as IL-1β, IL-6, and IL-8, by gingival keratinocytes and human gingival fibroblasts (HGF)....
Boron has significant impact on the mineral composition of teeth. Copine-7 (Cpne-7) is secreted by pre-ameloblasts and induces dentin formation via differentiation of mesenchymal cells of dental. The goal of this study was to see how boric acid affected the bioactivity and expression of Cpne-7, collagen type I in dental pulp-obtained stem cell. The...
Nails have been found to be a non-invasive and readily available tissue whose mineral content can change because of a change in dietary mineral intake. Thus, this study was undertaken to determine whether boron (B) supplementation would change the concentrations of some mineral elements in nails and whether these changes correlated with changes ind...
The reported beneficial effects of boron on mineralized tissues in animals and humans vary. Thus, a study was performed to assess whether the variability was the result of different forms of boron supplementation, method of supplementation, and increased adiposity of the rabbit experimental model.
Thirty-one female New Zealand White rabbits, (aged...
Amaç: Bu çalışmanın amacı; farklı yıkım paterni gösteren periodontal hastalığa sahip bireylerde nötrofil adezyon yolağının sıkı adezyon basamağında düzenleyici sitokinler olan Developmental Endothelial Locus-1(Del-1) ve Lymphocyte function-associated antigen-1(LFA-1) tükürük ve dişeti oluğu sıvısı seviyelerinin değerlendirilmesidir.Gereç ve Yönteml...
Papillon-Lefèvre sendromu, süt ve daimî dentisyonda erken diş kaybı ve palmaplantar hiperkeratoz ile karakterize otozomal resesif geçişli genetik bir hastalıktır. Bu olgu raporunda, Papillon-Lefèvre sendromlu (PLS) iki hastada, alveolar kemiği korumak için diş köklerinin dişeti altında bırakılması ve çekimlerini takiben çekim soketinin implant ile...
The effects of boron on the formation and maintenance of mineralized structures at the molecular level are still not clearly defined. Thus, a study was conducted using MC3T3-E1 cells to determine whether boron affected mRNA expressions of genes associated with bone/alveolar bone formation around the teethMC3T3-E1 (clone 4) cells were cultured in me...
Background/Objectives
In this in vitro study, the effects of Stromal cell‐derived factor‐1 (SDF‐1) was evaluated on the periodontal ligament‐Mesenchymal Stem Cells (pdl‐MSCs) functions.
Material and Methods
Real‐time cell analyzer‐single plate (RTCA‐SP) was employed for proliferation, and RTCA‐dual purpose (DP) was utilized for pdl‐MSCs migration...
Lipopolysaccharide is a potent virulence factor of Porphyromonas gingivalis and has been implicated predominant pathogen in the development and progression of periodontal diseases. The aim of this study was to determine the effect of Porphyromonas gingivalis lipopolysaccharide (Pg-LPS) on cementoblasts.
Cementoblast (OCCM-30) were evaluated prolife...
Objectives
To evaluate the efficiency of injectable platelet-rich fibrin (i-PRF) in accelerating canine tooth movement and to examine levels of the matrix metalloproteinase-8 (MMP-8), interleukin-1β (IL-1β), receptor activator of nuclear factor kappa-light-chain-enhancer of activated B cells ligand (RANKL), and osteoprotegerin (OPG) in the gingival...
Objectives: To evaluate the efficiency of injectable platelet-rich fibrin (i-PRF) in accelerating canine tooth movement and to examine levels of the matrix metalloproteinase-8 (MMP-8), interleukin-1b (IL-1b), receptor activator of nuclear factor kappa-light-chain-enhancer of activated B cells ligand (RANKL), and osteoprotegerin (OPG) in the gingiva...
Abstract
Objective: This review aims to critically evaluate the guidelines with respect to slow dentistry in clinical practice, education and
well-being for staff and students in the post COVID-19 era.
Subject: According to the data released on 14 October, cases of COVID-19 have been spotted in 215 different countries, with
38,688,225 total cases,...
Changes in the macro and trace element composition of saliva might be indicative for pathological changes in periodontal tissues. However, there is a lack of evidence in the literature showing associations between mineral elements and periodontal status. The aim of this study was to determine whether such associations occur. Totally, 190 systemical...
Bactericidal and detoxification effects of diode laser (DL) have been reported in periodontal treatment. The objective of this study was investigating the additional effect of DL with nonsurgical periodontal treatment on the red complex bacteria in type 2 diabetes mellitus (DM) patients with chronic periodontitis (CP). Sixty type 2 DM patients with...
This study examined the effect of curcumin on T-helper (Th17) and T-regulatory (Treg) cells regarding the mRNA of cytokines/mediators in the gingiva. Thirty-five male albino Wistar rats were divided into four groups: Group 1: periodontitis (n = 9); Group 2: periodontitis with curcumin treatment (n = 8); Group 3: periodontally healthy with curcumin...
Introduction
The aim of this study was to determine the effect of the continuous wave of condensation technique (CWCT) and the thermoplastic gutta-percha injection (TGI) technique on the messenger RNA (mRNA) expressions of heat shock proteins (HSPs) and mineralized tissue–associated proteins of the immortalized mouse cementoblasts (OCCM.30).
Metho...
Objective and background:
Developmental endothelial locus-1 (Del-1), lymphocyte function-associated antigen-1 (LFA-1), and interleukin 17 (IL-17) play critical roles in transendothelial migration of neutrophils in periodontal diseases. The aim of this study was to evaluate salivary Del-1, IL-17, and LFA-1 protein levels in patients with gingivitis...
Background:The aim of this study was to investigate levels of bone morphogenetic protein-2 (BMP-2), BMP-7, soluble receptor activator of nuclear factor-kB ligand (sRANKL) and osteoprotegerin (OPG) in the peri-implant crevicular fluid (PICF) of implants placed in both maxilla and mandible during the osseointegration period.Methods:Thirty-three patie...
Background:
Previous studies reported that nicotine, which is the prominent constituent of tobacco, has negative effects on periodontium cells. However, the precise role of nicotine in cementoblast functions remains unclear. In the present study, we investigated the effects of nicotine on the functions of cementoblasts (OCCM-30) in terms of prolif...
Introduction
This survey aimed to investigate the rate of students in dental schools that use various tobacco products and possible factors affecting their attitudes towards tobacco products.
Methods
The survey was conducted between May 1 and June 30, 2018 and participants were from ten dental schools in Turkey. First year students and fifth year...
Aim
Smoking is a well known risk factor for periodontitis and it has definitely negative impacts on the results of periodontal therapies. Nicotine, which is the main active component of tobacco, affects cell functions including proliferation, adhesion and differentiation of the cells. The aim of this study was to explore the effects of nicotine on...
Amelogenin (AMG) is a cell adhesion molecule that has an important role in the mineralization of enamel and regulates events during dental development and root formation. The purpose of the present study was to investigate the effects of recombinant human AMG (rhAMG) on mineralized tissue-associated genes in cementoblasts. Immortalized mouse cement...
Human mesenchymal stem cells (MSCs) are the most intensely studied and clinically used adult stem cell type. Conventional long-term cultivation of MSCs as a monolayer is known to result in a reduction of their functionality and viability. In addition, large volumes of cell culture
medium are required to obtain cell quantities needed for their clini...
Objectives:
The purpose of this study was to compare the proliferation and differentiation potential of mesenchymal stem cells (MSCs) derived from palatal adipose tissue (PAT) and lipoaspirated adipose tissue (LAT).
Materials and methods:
PATs were obtained from 2 healthy female patients undergoing surgery for gingival recession, and LATs were o...
The aim of this study was to evaluate the effects of diode laser biostimulation on cementoblasts (OCCM.30). A total of 40 root plates were obtained from healthy third molar teeth and assigned to the following two groups: (1) control group and (2) laser-treated group. Root plates were placed into the cell culture inserts, and OCCM.30 cells were seed...
The aims of this in vitro study are to compare the efficacy of different cleaning methods in removing debris of failed implants and to detect thermal changes of the implants treated by various scaling instruments. Twenty-seven failed implants and two unused implants as control were included to this study—group 1: plastic curette (P), group 2: titan...
Human gingival fibroblasts (HGFs) are the major constituents of the gingival tissues responsible for the synthesis and degradation of the connective tissue while actively participating in immune reactions and inflammation. The aim of this study was to test the impact of lipopolysaccharide (LPS) from Porphyromonas gingivalis (P. gingivalis) on human...
The purpose of this study was to induce experimental periodontitis in rats previously fed diets containing arginine silicate inositol (ASI) complex and examine the biochemical, immunological, and radiological effects. Fifty two 8-week-old female Sprague Dawley rats were equally divided into four groups. The control group included those fed a standa...
Aim: This finite elemental stress analysis (FEA) study was aimed to test the effect of fiber and metal posts on stress distribution in premolar teeth with endodontic–periodontal (EP) lesion. Methodology: Three FEA models representing different EP lesions (primary endodontic disease (PED), PED with periodontal involvement and true-combined) were cre...
Introduction:
The aim of this study was to evaluate the cytotoxic effects of three different light-cured orthodontic composites.
Material and methods:
Light Bond (Reliance orthodontic products), Grengloo (Ormco corporation), and Kurasper F (Kuraray Europe GmbH) were selected for the experiment. Specimens were prepared according to the manufactur...
Teeth can be lost due to many reasons including periodontitis, trauma and/or profound caries. Though current treatment modalities such as implantology or prosthesis may provide esthetical and functional restorations of missing teeth, new strategies are required to overcome limitations of traditional treatments towards more biologic treatment method...
In order to evaluate whether nonsurgical periodontal treatment with/without diode laser (DL) decontamination improves clinical parameters, the levels of IL-1β, IL-6, IL-8, intercellular adhesion molecule (ICAM), and vascular cell adhesion molecule (VCAM) in gingival crevicular fluid and metabolic control (HbA1c) in chronic periodontitis (CP) patien...
Abstract Regeneration of periodontal tissues aims to utilize tissue engineering techniques to restore lost periodontal tissues including the cementum, periodontal ligament and alveolar bone. Regenerative dentistry and its special field regenerative periodontology represent relatively new and emerging branches of translational stem cell biology and...
To evaluate the effects of bioresorbable fixation screws (BFSs) on human gingival fibroblast (HGF) and mouse osteoblast (MC3T3-E1) cell viability.
The KLS Martin SonicPins Rx, Synthes RapidSorb Cortex Screws, and Inion CPS Bioabsorbable Fixation System each were incubated in Dulbecco's Modified Eagle Medium for 72 hours according to ISO 10993-5 sta...
To evaluate the cytotoxicity of temporary luting cements on bovine dental pulp-derived cells (bDPCs).
Four different temporary cements were tested: Rely X Temp E (3M ESPE), Ultratemp (Ultradent), GC Fuji Temp (GC), and Rely X Temp NE (3M ESPE). The materials were prepared as discs and incubated in Dulbecco's modified eagle's culture medium (DMEM) f...
The purpose of this in vitro study was to compare the cytotoxicity of 0.5-4.5 origanum extract solution (OES), 2% chlorhexidine gluconate (CHX) and 5.25% sodium hypochlorite (NaOCl) with WST-1 test on human periodontal ligament (hPDL) fibroblasts.
About 0.5-4.5% OES, 2% CHX and 5.25% NaOCl solutions cytotoxicity was evaluated with cell culture test...
To evaluate the cytotoxicity of resin cements on dental pulp-derived cells (bDPCs). Bifix QM (BQM), Choice 2(C2), RelyX U200(RU200), Maxcem Elite(ME), and Multilink Automix(MA) were tested. The materials incubated in DMEM for 72 h. A real-time cell analyzer was used to evaluate cell survival. The statistical analyses used were one-way ANOVA and Tuk...
Objectives:
The aim of this study was to evaluate the cytotoxicity of four low-shrink composites with new monomer technology on the bovine dental pulp-derived cells (bDPCs).
Materials and methods:
Ten samples were prepared for each group composites, and the samples were immersed in 7 mL of culture medium for 72 h at 37°C to extract residual mono...
Background:
Cell-based therapy using mesenchymal stem cells (MSCs) seems promising to obtain regeneration of dental tissues. A comparison of tissue sources, including periodontal ligament (PDL) versus pulp (P), could provide critical information to select an appropriate MSC population for designing predictable regenerative therapies. The purpose o...
Objective:
The aim of this study was to investigate the effects of diode laser(DL) decontamination as an adjunct to non-surgical periodontal treatment on the clinical periodontal parameters, gingival crevicular fluid(GCF) IL-1β,IL-6 and IL-8 levels and metabolic control of type-2 diabetes mellitus (DM) patients with chronic periodontitis(CP).
M...
Purpose: The aim of this study was to evaluate the cytotoxic effects of 6 different orthodontic bracket types on human gingival fibroblasts (HGFs) using the xCELLigence system.
Methods: The orthodontic brackets used in this study were gold-plated steel (Apollo Gold), titanium (Rematitan), stainless steel (Equilibrium 2), lucid ice (Inspire ICE), m...
We aimed to evaluate the effects of ionizing radiation on the proliferation of gingival fibroblasts and the expressions of proinflammatory cytokines and matrix metalloproteinase-1 (MMP-1), tissue inhibitor of matrix metalloproteinase-1 (TIMP-1) and type I collagen (type I Col) mRNA transcripts. Gingival fibroblasts were treated with radiation doses...
Objective:
The comparison of tissue sources including ‘‘periodontal ligament (PDL) versus pulp (P)’’ could provide critical information to select appropriate MSCs population in designing predictable regenerative periodontal therapies. The purpose of this study was to compare proliferation, stemness and mesenchymal stem cell specific and mineraliz...
Background and Objective
The implant surface plays a major role in the biological response to titanium dental implants. The aim of this study was to investigate levels of soluble receptor activator of nuclear factor-κB ligand (sRANKL), osteoprotegerin (OPG), bone morphogenetic protein-2 (BMP-2) and -7 (BMP-7) in the peri-implant crevicular fluid (P...
The utility of adult stem cells for bone regeneration may be an attractive alternative in the treatment of extensive injury, congenital malformations, or diseases causing large bone defects. To create an environment that is supportive of bone formation, signals from molecules such as the bone morphogenetic proteins (BMPs) are required to engineer f...
Objective: Hemangiomas are common benign vascular lesions and they can occur in the mouth including lips, tongue, buccal mucosa, and palate. Despite their benign origin and behavior, hemangiomas in the oral cavity are always of clinical importance to the dental profession. Since hemangiomas can lead to intra-operative bleeding, excision with diode...
Objective: Soft tissue lasers, like diode laser, are getting popular among clinicians due to their potential value in pre-restorative and pre-orthodontic gingival procedures. The ability of diode laser to control moisture, facilitate homeostasis provides comfort to the clinicians during periodontal surgeries. Moreover, its postoperative advantages...
Objective: Diode laser is a viable alternative device for various periodontal soft tissue procedures such as frenectomy, gingivectomy and gingivoplasty. Dry surgical field, reduced swelling, edema, pain and scarring, faster healing response and increased patient acceptance are considered as reasons for preferring the laser. In this case series, we...
Abstract The purpose of this study was to investigate adhesion, proliferation and type I collagen (COL I) mRNA expression of gingival fibroblasts on different membranes used in periodontal applications. Collagen (C), acellular dermal matrix (ADM), and polylactic acid; polyglycolic acid; lactide/glycolide copolymer (PLGA) biodegradable membranes wer...
Oral lichen planus (OLP) is a chronic inflammatory disease that is frequently detected in oral tissues. The aim of our study was to identify the prevalence of the detection of periodontopathogenic microorganisms (Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, Prevotella intermedia, Tannerella forsythia and Treponema denticola in O...
Achieving of moisture control especially gingival bleeding control is great challenge in clinical practice. Various hemostatic agents and techniques have been promoted for bleeding control during dental operation. But few studies have focused on the cytotoxicity of hemostatic solutions.
The aim of this study was to evaluate cytotoxic effect of hemo...
The aim of the study was to evaluate the efficiency of an Er, Cr:YSGG laser to remove the biofilm of the contaminated implant surfaces and to examine the possible alterations on the surface structure after laser irradiation.
Methods: Nine failed SLA surfaced dental implants were used for laser irradiation and one unused implant served as control. O...
Objective: Cell-based therapy employing mesenchymal stem cells (MSCs) seems promising to obtain periodontal regeneration. One of the critical components of periodontal tissue engineering is the selection of the appropriate MSCs population. The purpose of this study was to compare osteogenic and proliferative potentials of mesenchymal stem cells iso...
To investigate cell viability and gene expression of cementoblasts (OCCM.30) exposed to extractable components released by resin-based sealers with different chemical composition Hybrid Root Seal (HRS), SimpliSeal (SS), Real Seal (RS) and AH Plus (AH) and by a MTA-based sealers Tech Biosealer Endo (TBE).
Discs of all materials were prepared and all...
Objective:
Oral lichen planus (OLP) is a chronic inflammatory disease of unknown aetiology. The aim of this study was to investigate matrix metalloproteinase (MMP)-1, MMP-9, and MMP inhibitor-1 (TIMP-1) levels in gingival crevicular fluid (GCF) by enzyme-linked immunosorbent assay and to investigate MMP-1, MMP-9, and TIMP-1 levels in gingival tiss...