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Publications (5)
Clonal growth and competition underlie processes of key relevance in etiology, progression and therapy response across all cancers. Here, we demonstrate a novel experimental approach, based on multi-color, fluorescent tagging of cell nuclei, in combination with picoliter droplet deposition, to study the clonal dynamics in two- and three-dimensional...
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The Clonogenic Survival Assay (CSA) is pivotal in gauging cell survival and proliferative capacity in oncology research. However, it’s limited by its binary output and its time- and labor-intensive nature. We developed LeGO-CSA, a refined version of CSA, utilizing imaging of cell nuclei tagged with fluorescent markers which encode re...
Clonal proliferation dynamics within a tumor channels the course of tumor growth, drug response and activity. A high-throughput image screening technique is required to analyze and quantify the spatiotemporal variations in cell proliferation and influence of chemotherapy on clonal colonies. We present two protocols for generating spatial, Lentivira...
Botulinum neurotoxins represent a revolution in cosmetic science because of their extraordinary and long-term anti-wrinkle properties. Nevertheless, high neurotoxicity severely limits their usage. Therefore, design and validation of new non-toxic molecules which mimics the Botox are needed. Here, acetyl octapeptide-3 is used which mimics the effect...
Serum proteins can generally be considered a good source for the illness' indication and are precious resources to detect diseases such as inflammation, cancer, diabetes, malnutrition, cardiovascular diseases, Alzheimer's, other autoimmune diseases, and infections. However, one of the biggest difficulties for proteomic studies is that the majority...
