Seher Abbas- Dr. rer. nat (Doctor of Science)
- Ph.D. at Carl von Ossietzky University of Oldenburg
Seher Abbas
- Dr. rer. nat (Doctor of Science)
- Ph.D. at Carl von Ossietzky University of Oldenburg
Digitalizing Pharma.
About
16
Publications
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Introduction
Career Interests: | Vision and blindness research | Cell Signalling | Sensory Diseases | Animal Models | Clinical Research |
I have a strong background in biophysics, biochemistry and molecular biology. Over 3 years of research in protein biochemistry. Experienced in optimizing new protocols, fast learner, and able to communicate efficiently with students, scientists and business professionals.
Current institution
Additional affiliations
January 2014 - April 2016
June 2011 - August 2011
September 2008 - June 2012
Publications
Publications (16)
The guanylyl cyclase activating protein, GCAP1, activates photoreceptor membrane guanylyl cyclase (RetGC) in the light, when free Ca²⁺ concentrations decline, and decelerates the cyclase in the dark, when Ca²⁺ concentrations rise. Here, we report a novel mutation, G86R, in the GCAP1 (GUCA1A) gene in a family with a dominant retinopathy. The G86R su...
G protein coupled receptor kinase 1 (GRK1) or rhodopsin kinase is under specific control of the neuronal Ca2+-sensor protein recoverin, which is a critical feedback mechanism responsible for the modulation of the shape and sensitivity of the rod cell photoresponse. This process requires the precise matching of interacting protein surfaces and the d...
Guanylate Cyclase activating protein 1 (GCAP1) mediates the Ca²⁺-dependent regulation of the retinal Guanylate Cyclase (GC) in photoreceptors, acting as a target inhibitor at high [Ca²⁺] and as an activator at low [Ca²⁺]. Recently, a novel missense mutation (G86R) was found in GUCA1A, the gene encoding for GCAP1, in patients diagnosed with cone-rod...
Diverse and complex molecular recognitions are central elements of signal transduction cascades. The strength and nature of these interaction modes can be determined by different experimental approaches. Among those, Isothermal titration calorimetry (ITC) offers certain advantages by providing binding constants and thermodynamic parameters from tit...
The functional performance of a cell depends on how macromolecules, in particular proteins, come together in a precise orientation, how they assemble into protein complexes and interact with each other. In order to study protein-protein interactions at a molecular level, a variety of methods to investigate these binding processes yield affinity con...
Genetic heterogeneity leading to retinal disorders impairs biological processes by causing for example severe disorder of signal transduction in photoreceptor outer segments. A normal balance of the second messenger homeostasis in photoreceptor cells seems to be a crucial factor for healthy and normal photoreceptor function. Genes like GUCY2D codin...
Gene expression is tightly regulated in time and space through a multitude of factors consisting of signaling molecules. Soluble N-ethylmaleimide-sensitive-factor attachment protein receptors (SNARE) are membrane proteins responsible for the intercellular trafficking of signals through endocytosis and exocytosis of vesicles. Altered expression of S...
Wilcoxon test for Synaptobrevin 2 and Syntaxin1A gene.
Tukey and Kruskall-Wallis tests for VAMP2 and STX1A gene
expression.
Spearman correlations between VAMP2 with STX1A
expression.
Mann-Whitney test for VAMP2 and STX1A analysis in low and
high-grade. tumors.
Objective: Genetic factors contribute to the high rates with breast cancer patients. Our objective was to screen themutations in the BRCA 1 gene in exon 20. Design: A diagnostic study. Place and Duration of Study: The study was carried out inmolecular biology lab, Department of Zoology, GC University Lahore and Institute of Molecular Biology and Bi...
Goiter refers to any visible enlargement of the thyroid gland and it is one of the major health problems in human beings. The present study was conducted to determine the prevalence of goiter and its predisposing as well as precipitating factors among people of district Bahawalpur. The question based review chart was carried out at Bahawalpur Victo...
A total of 2100 rectal faecal samples were collected from Civil Veterinary Hospital, Bahawalnagar, Slaughter house, Bahawalnagar and Private cattle Farmers, in and around Bahawalnagar under different managemental practices. All the faecal samples were screened for the presence of different eimerian oocysts and 1440 were found positive for Eimeria s...
Five hundred blood samples were processed at Parasitology Laboratory, University of Veterinary and Animal Sciences, Lahore and the Islamia University of Bahawalpur. These samples were examined for the presence of different blood protozoans such as Theileria, Babesia and Trypnosoma in buffalo's blood. The study revealed that in Rahim Yar Khan the mo...
Questions
Questions (8)
hello everyone,
Can someone tell me which software will work in windows 10 to download pymol movies?
Thank you in advance.
Hi everyone,
I am having startup problem with biacore 2000. an error 92:188 pops up when i start system. System was working fine before.
Do i have to uninstall and reinstall BIA control software? would it be helpful?
Hello everyone,
I titrated my protein WT and mutant against Ca. i received three kd vales using 3 site model. that we already know is fine. all KD values were obviously different in case of wild-type like kd1=2, kd2=17 and kd3=120. now incase of mutant all kds shifted like kd1=17, kd2 400, kd3=7. binding affinity reduced because of mutation but what i expected all kds should remain in the same order as for wild-type. But here we don't know which kd is coming from which EF-hand. that is true. my concern is if any EF-hand is saturated first it should come first as kd1 and so on. but what i observed incase of mutant that order is disturbed like kd1 is 17 and kd3 is 7 and kd2 is 400. i believe it should come as kd1= 7, kd2=17 and kd3 as 400 based on EF-hand saturation pattern. maybe there is something that i am missing. Can anyone explain this.
thank you.
Hello everyone,
My nanoparticles are linked with 4,4 biphenol dithiol linkers. and i need -COOH groups on particle surface in addition to free thiol groups. I need -COOH groups for (EDC/NHS) protein conjugation to particles. Can any one explain how can i bring -COOH groups to particle suface while i dont lose many free thiol groups? using mercaptosuccinic acid would be sufficient or should i try long carbon chains with -COOH groups? then how wold i be sure i still am left with some free thiol groups because i need them for self assembled monolayers to glass surface.
Can anyone share me protocol methods?
Thnak you
Hello everyone,
i want to use an aptamer to my gold nanoparticles to attach protein later (for high specificity). Can anyone explain me which aptamer should i use as my protein is GST fused. So i need an aptamer against anti-GST antibody (epitope). and where can i buy one?
Thank you.
Hi guys,
i am just curious are there any methods or assays (bioinfo tools) to access protein internal rotation coming from single amino acid. My protein has a mutation, replacement of Glycine to arginine, as glycine is significantly small than highly basic and large side chained arginine. i am wondering how gross that effect could be incase of steric hinderance, lateral bonds formation and free internal rotation.
another important thing glycine was present in middle of two lysine's (KGK) so now (KRK) can also be effected due to surrounding basic lysin's.
any clues please write
Thank you.
Can anyone share your experience. Thank you.
In my west blot results of a membrane protein, I observed weak and missing signals for pellet instead supernatant signals were highly strong.
