Sébastien Thomine

Sébastien Thomine
French National Centre for Scientific Research | CNRS · Institute for Integrated Biology of the Cell

About

117
Publications
24,864
Reads
How we measure 'reads'
A 'read' is counted each time someone views a publication summary (such as the title, abstract, and list of authors), clicks on a figure, or views or downloads the full-text. Learn more
9,895
Citations

Publications

Publications (117)
Article
Full-text available
Transition metals are essential for a wealth of metabolic reactions, but their concentrations need to be tightly controlled across cells and cell compartments, as metal excess or imbalance has deleterious effects. Metal homeostasis is achieved by a combination of metal transport across membranes and metal binding to a variety of molecules. Gene dup...
Preprint
Full-text available
Transition metals are essential for a wealth of metabolic reactions, but their concentrations need to be tightly controlled across cells and cell compartments, as metal excess or imbalance has deleterious effects. Metal homeostasis is achieved by a combination of metal transport across membranes and metal binding to a variety of molecules. Gene dup...
Article
To provide sustainable, sufficient, and nutritious food to a growing population is a major challenge for agriculture and the plant research community. In this respect the mineral micronutrient content of food crops deserves particular attention. Micronutrient deficiencies in cultivated soils and plants are a global problem which adversely affects c...
Preprint
Manganese is an essential metal for plant growth. The most important Mn-containing enzyme is the Mn4CaO5 cluster that catalyses water oxidation in Photosystem II. Mn deficiency primarily affects photosynthesis, while Mn excess is generally toxic. Mn excess and deficiency were studied in the liverwort Marchantia polymorpha, an emerging model ideally...
Preprint
Full-text available
miR408 and miR398 are two conserved microRNAs which expression is activated by the SPL7 transcription factor in response to copper starvation. We identified these two microRNAs families as upregulated in Arabidopsis thaliana and Solanum lycopersicum roots infected by root-knot nematodes. These endoparasites induce the dedifferentiation of a few roo...
Article
Iron (Fe) is involved in multiple processes that contribute to the maintenance of the cellular homeostasis of all living beings. In photosynthetic organisms, Fe is notably required for photosynthesis. Although iron is generally abundant in the environment, it is frequently poorly bioavailable. This review focuses on the molecular strategies that ph...
Article
Full-text available
In North Africa, barley (Hordeum vulgare L) is the second most cultivated cereal. In Tunisia, barley is cultivated in mining areas with possible Cd soil contamination. The accumulation of Cd was studied in the 36 most cultivated North African barley cultivars cultured during 6 months on control soil and on soil containing 10 ppm of Cd. Cadmium did...
Article
Full-text available
Iron is an essential nutrient for most forms of life, and this is particularly the case for photosynthetic organisms such as plants and algae. Iron cofactors including haem and FeS clusters play key roles in photosynthetic electron transfer, as well as in electron transfer in the mitochondria to make ATP. The iron is, along with other mineral nutri...
Article
Full-text available
Plants spend most of their life oscillating around 1–3 Hz due to the effect of the wind. Therefore, stems and foliage experience repetitive mechanical stresses through these passive movements. However, the mechanism of the cellular perception and transduction of such recurring mechanical signals remains an open question. Multimeric protein complexe...
Article
Cadmium is a non-essential trace metal, which is highly toxic to nearly all living organisms. Soil pollution causes Cd contamination of crops, thereby rendering plant products responsible for the chronic low level Cd over-exposure of numerous populations in the world. For this reason, Cd accumulation in plants has been studied for about five decade...
Article
Full-text available
Ion transporters are key players of cellular processes. The mechanistic properties of ion transporters have been well elucidated by biophysical methods. Meanwhile, the understanding of their exact functions in cellular homeostasis is limited by the difficulty of monitoring their activity in vivo. The development of biosensors to track subtle change...
Article
The Anthropocene epoch is associated with the spreading of metals in the environment increasing oxidative and genotoxic stress on organisms. Interestingly, about 500 plant species growing on metalliferous soils acquired the capacity to accumulate and tolerate tremendous amount of nickel in their shoots. The wide phylogenetic distribution of these s...
Article
To ensure the success of the new generation in annual species, the mother plant transfers a large proportion of the nutrients it has accumulated during its vegetative life to the next generation through its seeds. Iron (Fe) is required in large amounts to provide the energy and redox power to sustain seedling growth. However, free Fe is highly toxi...
Article
During development, tissues are submitted to high variation of compression and tension forces. The roles of the cell wall, the cytoskeleton, the turgor pressure and the cell geometry during this process have received due attention. In contrast, apart from its role in the establishment of turgor pressure, the involvement of the plasma membrane as a...
Article
Full-text available
Nutrient recycling and mobilization from organ to organ all along the plant lifespan is essential for plant survival under changing environments. Nutrient remobilization to the seeds is also essential for good seed production. In this review, we summarize the recent advances made to understand how plants manage nutrient remobilization from senescin...
Preprint
Full-text available
Throughout their life, plants are submitted to recurrent cyclic mechanical loading due to wind. The resulting passive oscillation movements of stem and foliage is an important phenomenon for biological and ecological issues such as photosynthesis optimization and thermal exchange. The induced motions at plant scale are well described and analyzed,...
Preprint
Ion transporters are key players of cellular processes. The mechanistic properties of ion transporters have been well elucidated by biophysical methods. Meanwhile the understanding of their exact functions in the whole cell homeostasis is limited by the difficulty to monitor their activity in vivo . The development of biosensors to track subtle cha...
Article
Full-text available
Chloroplasts, mitochondria, and vacuoles represent characteristic organelles of the plant cell, with a predominant function in cellular metabolism. Chloroplasts are the site of photosynthesis and therefore basic and essential for photoautotrophic growth of plants. Mitochondria produce energy during respiration and vacuoles act as internal waste and...
Article
Full-text available
Micronutrient deficiencies affect a large part of the world population. They are mostly due to the consumption of grains with insufficient content of Fe or Zn. Both de novo uptake by roots and recycling from leaves may provide seeds with nutrients. Autophagy, which is a conserved mechanism for nutrient recycling in eukaryotes, was shown to be invol...
Preprint
Full-text available
The Anthropocene epoch is associated with the spreading of metals in the environment increasing oxidative and genotoxic stress on living organisms. Once regarded as a curiosity, plants hyperaccumulating metals are now envisioned as an opportunity to remediate metal contaminated soils. About 500 plant species adapted to metalliferous soils acquired...
Article
Mechanosensitive (MS) channels behave as micro‐probes that transduce mechanical tension into electric and ion signals. The plasma membrane anion‐permeant channel AtMSL10 belongs to the first family of MS channels (MscS‐LIKE) that has been characterized in Arabidopsis thaliana. In the same membrane, a rapidly activated calcium MS channel activity (R...
Article
Full-text available
During seed germination, iron (Fe) stored in vacuoles is exported by the redundant NRAMP3 and NRAMP4 transporter proteins. A double nramp3 nramp4 mutant is unable to mobilize Fe stores and does not develop in the absence of external Fe. We used RNA sequencing to compare gene expression in nramp3 nramp4 and wild type during germination and early see...
Preprint
Full-text available
Micronutrient deficiencies affect a large part of the world population. They are mostly due to the consumption of grains with insufficient content of Fe or Zn. It is therefore important to improve our knowledge of the mechanisms of micronutrient loading to seeds. Nutrient loaded in seeds originate either from de novo uptake by roots or recycling fr...
Preprint
Full-text available
During seed germination, iron (Fe) stored in vacuoles is exported by the redundant NRAMP3 and NRAMP4 transporter proteins. A double nramp3 nramp4 mutant is unable to mobilize Fe stores and does not develop in the absence of external Fe. We used RNA sequencing to compare gene expression in nramp3 nramp4 and wild type during germination and early see...
Preprint
The Anthropocene epoch is associated with the spreading of metals in the environment increasing oxidative and genotoxic stress on living organisms [1,2]. Metalliferous soils such as serpentine can host peculiar plants called hyperaccumulators due to their capacity to accumulate tremendous amount of metal in their shoot (e.g. >0.1% of dry weight for...
Article
“Too much of a good thing” perfectly describes the dilemma that living organisms face with metals. The tight control of metal homeostasis in cells depends on the trafficking of metal transporters between membranes of different compartments. However, the mechanisms regulating the location of transport proteins are still largely unknown. Developing A...
Chapter
Transmission Electron Microscopy equipped with Energy Dispersive Spectroscopy (TEM-EDS) is known to be a powerful tool to study metal speciation in biological and environmental samples. The characterisation of different metal pools present in organisms is indeed essential to understand optimal development under various environmental constraints. Fo...
Article
ATP harbors three phosphoric groups linked by two highly energetic phospho-anhydride bonds. The exergonic hydrolysis of the phospho-anhydride bonds of ATP can be used in biochemical reactions to couple the released energy to another endergonic reaction. Thus, ATP is a timing belt of cellular metabolism and energy distribution in cells. The cytosoli...
Article
Full-text available
In plant cells, anion channels and transporters are essential for key functions such as nutrition, ion homeostasis and resistance to biotic or abiotic stresses. We characterized AtCLCg, a member of the CLC family in Arabidopsis localized in the vacuolar membrane. When grown on NaCl or KCl, atclcg knock-out mutants showed a decrease in biomass. In t...
Article
Full-text available
To improve seed iron (Fe) content and bioavailability, it is crucial to decipher the mechanisms that control Fe storage during seed development. In Arabidopsis thaliana seeds, most Fe is concentrated in insoluble precipitates with phytate in the vacuoles of cells surrounding the vasculature of the embryo. AtNRAMP3 and AtNRAMP4 function redundantly...
Article
Full-text available
Each essential transition metal plays specific roles in metabolic processes and has to be selectively transported. Living organisms need to discriminate between essential and non-essential metals such as cadmium (Cd(2+) ), which is highly toxic. However, transporters of the NRAMP family, which are involved in metal uptake and homeostasis, generally...
Article
The ability to characterize the structure of metal centers beyond their primary ligands is important to understanding their chemistry. High magnetic-field pulsed electron double resonance detected NMR (ELDOR-NMR) is shown to be a very sensitive approach to measuring the multinuclear NMR spectra of the nuclei surrounding Mn(II) ions. Resolved spectr...
Article
The manganese(ii) speciation in intact cells of D. radiodurans, E. coli, S. cerevisiae and Arabidopsis thaliana seeds was measured using high-field electron paramagnetic resonance techniques. The majority of the Mn(ii) ions in these organisms were six-coordinate, bound predominately by water, phosphates and nitrogen-based molecules. The relative di...
Article
Full-text available
Eukaryotic anion/proton exchangers of the CLC (chloride channel) family mediate anion fluxes across intracellular membranes. The Arabidopsis thaliana anion/proton exchanger AtCLCa is involved in vacuolar accumulation of nitrate. We investigated the role of AtCLCa in leaf guard cells, a specialized plant epidermal cell that controls gas exchange and...
Article
Full-text available
Nickel is an economically important metal and phytotechnologies are being developed to limit the impact of nickel mining on the environment. More than 300 plant species are known to hyperaccumulate nickel. However, our knowledge of the mechanisms involved in nickel accumulation in plants is very limited because it has not yet been possible to study...
Article
Full-text available
Siderophores are specific ferric iron chelators synthesized by virtually all micro-organisms in response to iron deficiency. We have previously shown that they promote infection by the phytopathogenic enterobacteria Dickeya dadantii and Erwinia amylovora Siderophores have also the ability to activate plant immunity. We have used CATMA microarrays t...
Article
Full-text available
Seed formation is an important step of plant development which depends on nutrient allocation. Uptake from soil is an obvious source of nutrients which mainly occurs during vegetative stage. Because seed filling and leaf senescence are synchronized, subsequent mobilization of nutrients from vegetative organs also play an essential role in nutrient...
Article
Zinc plays a central role in all living cells as a cofactor for enzymes and as a structural element enabling the adequate folding of proteins. In eukaryotic cells, metals are highly compartmentalized and chelated. Although essential to characterize the mechanisms of Zn(2+) homeostasis, the measurement of free metal concentrations in living cells ha...
Article
Full-text available
While an excess of metals such as zinc, cadmium or nickel (Ni) is toxic for most plants, about 500 plant species called hyperaccumulators are able to accumulate high amounts of these metals. These plants and the underlying mechanisms are receiving an increasing interest because of their potential use in sustainable biotechnologies such as biofortif...
Article
Full-text available
Seeds are a crucial stage in plant life. They contain the nutrients necessary to initiate the development of a new organism. Seeds also represent an important source of nutrient for human beings. Iron (Fe) and zinc (Zn) deficiencies affect over a billion people worldwide. It is therefore important to understand how these essential metals are stored...
Article
Cadmium (Cd) is highly toxic to plants causing growth reduction and chlorosis. It binds thiols and competes with essential transition metals. It affects major biochemical processes such as photosynthesis and the redox balance, but the connection between cadmium effects at the biochemical level and its deleterious effect on growth has seldom been es...
Article
Full-text available
Practically all human populations are environmentally exposed to cadmium (Cd), mostly through plant-derived food. A growing body of epidemiological evidence suggests that there is no margin of safety between current Cd exposure levels and the threshold for adverse health effects and, hence, there is an urgent need to lower human Cd intake. Here we...
Article
Plant anion channels allow the efflux of anions from cells. They are involved in turgor pressure control, changes in membrane potential, organic acid excretion, tolerance to salinity and inorganic anion nutrition. The recent molecular identification of anion channel genes in guard cells and in roots allows a better understanding of their function a...
Article
Full-text available
Plant cell vacuoles are diverse and dynamic structures. In particular, during seed germination, the protein storage vacuoles are rapidly replaced by a central lytic vacuole enabling rapid elongation of embryo cells. In this study, we investigate the dynamic remodeling of vacuolar compartments during Arabidopsis seed germination using immunocytochem...
Chapter
Full-text available
Iron is an essential micronutrient for most living organisms. Paradoxically, although iron is abundant in many soils, iron availability is very often limiting for plant growth. In addition, iron is potentially highly toxic to cells. Therefore, iron homeostasis needs to be tightly regulated. This chapter focuses on the iron transport pathways dedica...
Article
Full-text available
The specific transport of metal ions, mediated by membrane-localized metal transporters, is of fundamental importance in all eukaryotes. Genome-wide analysis of metal transporters was undertaken, making use of whole genome sequences of the green alga Chlamydomonas reinhardtii, the moss Physcomitrella patens, the lycophyte Selaginella moellendorffii...
Article
In plant cells, anion channels and transporters are essential for key functions such as nutrition, resistance to biotic or abiotic stresses, and ion homeostasis. In Arabidopsis, members of the chloride channel (CLC) family located in intracellular organelles have been shown to be required for nitrate homeostasis or pH adjustment, and previous resul...
Article
Full-text available
Plant genomes code for channels involved in the transport of cations, anions and uncharged molecules through membranes. Although the molecular identity of channels for cations and uncharged molecules has progressed rapidly in the recent years, the molecular identity of anion channels has lagged behind. Electrophysiological studies have identified S...
Article
The crystal structure of a protein channel provides clues about the mechanisms that control the closure of pores found in the epidermis of plant leaves. Excitingly, the protein channel folds in a way never seen before. See Article p.1074
Article
Nitrate, the major nitrogen source for plants, can be accumulated in the vacuole. Its transport across the vacuolar membrane is mediated by AtCLCa, an antiporter of the chloride channel (CLC) protein family. In contrast to other CLC family members, AtCLCa transports nitrate coupled to protons. Recently, the different behaviour towards nitrate of CL...
Article
Anion channels/transporters are key to a wide spectrum of physiological functions in plants, such as osmoregulation, cell signaling, plant nutrition and compartmentalization of metabolites, and metal tolerance. The recent identification of gene families encoding some of these transport systems opened the way for gene expression studies, structure-f...
Article
Full-text available
Manganese (Mn) is an essential element, acting as cofactor in numerous enzymes. In particular, a Mn cluster is indispensable for the function of the oxygen-evolving complex of photosystem II. Metal transporters of the Natural Resistance-Associated Macrophage Protein (NRAMP) family have the ability to transport both iron and Mn. AtNRAMP3 and AtNRAMP...
Chapter
Stomata form a crucial interface between plants and the atmosphere and are essential to the control of water balance in plants. Prolonged soil dehydration or a drop in atmospheric relative humidity lead to a decrease in biomass production, plant water loss and eventually death. For plants to survive, it is essential that stomata close in response t...
Article
Full-text available
Ferritins are major players in plant iron homeostasis. Surprisingly, their overexpression in transgenic plants led only to a mod