Sebastiaan Van Nuffel

Sebastiaan Van Nuffel
Maastricht University | UM

PhD

About

14
Publications
4,317
Reads
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125
Citations
Additional affiliations
December 2020 - present
Maastricht University
Position
  • Professor (Assistant)
January 2020 - December 2020
Pennsylvania State University
Position
  • Professor
January 2019 - January 2020
Pennsylvania State University
Position
  • PostDoc Position
Education
September 2013 - October 2016
September 2011 - September 2013
University of Antwerp
Field of study
  • Chemistry
September 2007 - September 2011
University of Antwerp
Field of study
  • Chemistry

Publications

Publications (14)
Article
Imaging mass spectrometry (IMS) has become a powerful tool to characterise the spatial distribution of biomolecules in thin tissue sections. In the case of matrix-assisted laser desorption ionisation (MALDI) IMS, homogeneous matrix deposition is critical to produce high quality ion images and sublimation in particular has shown to be an excellent m...
Article
Full-text available
Pulmonary arterial hypertension (PAH) is a rare and deadly disease affecting roughly 15-60 people per million in Europe with a poorly understood pathology. There are currently no diagnostic tools for early detection nor does a curative treatment exist. The lipid composition of arteries in lung tissue samples from human PAH and control patients were...
Article
Variants of the SLC24A5 gene, which encodes a putative potassium-dependent sodium–calcium exchanger (NCKX5) that most likely resides in the melanosome or its precursor, affect pigmentation in both humans and zebrafish (Danio rerio). This finding suggests that genetic variations influencing human skin pigmentation alter melanosome biogenesis via ion...
Article
Full-text available
Skeletal stem cells (SSCs, or mesenchymal stromal cells typically referred to as mesenchymal stem cells from the bone marrow) are a dynamic progenitor population that can enter quiescence, self-renew or differentiate depending on regenerative demand and cues from their niche environment. However, ex vivo, in culture, they are grown typically on har...
Article
Time-of-flight secondary ion mass spectrometry (TOF-SIMS) imaging is a surface analysis technique that identifies and spatially resolves the chemical composition of a sample with a lateral resolution of less than 1 μm. Depth analyses can also be performed over thicknesses of several microns. In the case of a painting cross section, for example, TOF...
Article
Certain objects and know‐how have cultural significance in our societies, that is, they are inherited from a common past and are meant to be preserved and passed down from generation to generation. These can be works of art such as frescoes, paintings or jewelry, but also statues, ruins, and everyday objects such as pottery, tools, remedies, and co...
Article
Time-of-flight secondary ion mass spectrometry (TOF-SIMS) imaging is a surface analysis technique that identifies and spatially resolves the chemical composition of a sample with a lateral resolution of less than 1 μm. Depth analyses can also be performed over thicknesses of several microns. In the case of a painting cross section, for example, TOF...
Chapter
Over the past couple of years, imaging mass spectrometry (IMS) has arisen as a powerful tool to answer research questions in the biomedical field. Imaging mass spectrometry allows for label-free chemical imaging by providing full molecular information. The IMS technique best positioned for cell and tissue analysis is time-of-flight secondary ion ma...
Article
Full-text available
Molecular imaging has the potential to unearth important spatial and temporal relationships in biological systems, including intercellular signaling as well as environmentally-cued morphological changes. Various chemical imaging techniques show functional groups, molecular weights, etc., but no individual technique to date has the ability to simult...
Thesis
Full-text available
Time-of-flight secondary ion mass spectrometry (ToF-SIMS) has proven its ability to characterise (in)organic surfaces, and is increasingly used for the characterisation of biological samples such as single cells. By combining ion imaging and molecular depth profiling it is possible to render 3D chemical images, which provides a novel, label-free wa...
Raw Data
This deposit contains the raw ToF-SIMS data of freeze-dried cells used in Van Nuffel, Sebastiaan, et al. ”Multivariate analysis of 3D ToF-SIMS images: method validation and application to cultured neuronal networks.” Analyst 141.1 (2016): 90-95. URI https://rdmc.nottingham.ac.uk/handle/internal/54 http://dx.doi.org/10.17639/nott.51
Article
Full-text available
Advanced data analysis tools are crucial for the application of ToF-SIMS analysis to biological samples. Here, we demonstrate that by using a training set approach principal components analysis (PCA) can be performed on large 3D ToF-SIMS images of neuronal cell cultures. The method readily provides access to sample component information and signifi...
Article
Time-of-Flight Static Secondary Ion Mass Spectrometry excels in probing the molecular composition of the outer monolayer of flat samples with a lateral resolution in the sub-μm range. However, the method faces significant methodological problems in the case of non-conducting samples with high topography or surface curvature, such as fibres, yarns o...

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