Sebastià Puig

Sebastià Puig
Universitat de Girona | UDG · LEQUIA Laboratory of Chemical and Environmental Engineering

Associate professor Serra Húnter at the University of Girona (Spain)

About

191
Publications
42,797
Reads
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4,654
Citations
Introduction
Climate change and future depletion of resources are two of the most important environmental challenges that humankind have ever faced. The following years I pretend to tackle the root of these issues by putting forward a resilience and sustainable technology based electron-driven microbial reactions. Dr. Puig aims to give a second chance to contaminated water and recalcitrant carbon dioxide (CO2) streams.
Additional affiliations
January 2019 - present
Universitat de Girona
Position
  • Professor (Associate)
September 2010 - December 2010
Ghent University
Position
  • Scientific visitor
Description
  • Development of three microbiological gas treatment systems for mitigating anthropogenic point sources of nitrous oxide (N2O), with focus on the wastewater treatment sector
January 2010 - December 2018
Universitat de Girona
Position
  • Professor (Assistant)

Publications

Publications (191)
Article
Several regions around the world present high levels of nitrate in groundwater. Due to its toxicity, nitrate must be removed before the groundwater is used as drinking- water. This study assessed how a denitrifying bioelectrochemical system could be operated to treat nitrate- polluted groundwater. It evaluated the cathode potential (from +597 to -7...
Article
Full-text available
Electrified biotrickling filters represent sustainable microbial electrochemical technology for treating organic carbon-deficient ammonium-contaminated waters. However, information on the microbiome of the conductive granule bed cathode remains inexistent. For uncovering this black box and for identifying key process parameters, minimally invasive...
Article
Anaerobic gas fermentation is a promising approach to transform carbon dioxide (CO2) into chemical building blocks. However, the main operational conditions to enhance the process and its selectivity are still unknown. The main objective of this study was to trigger chain elongation from a joint perspective of thermodynamic and experimental assessm...
Article
Wastewater treatment processes energy and emissions issues led researchers to investigate more sustainable alternative technologies, aimed at achieving effective contaminants removal with simultaneous resources recovery (i.e., energy). The combination of microbial fuel cell (MFC) technology with microalgal-based processes in a photo-MFC (PhMFC) cou...
Article
Hydrogen-oxidizing bacteria (HOBs) are prime candidates for the sustainable production of microbial protein because of their high nutritional value and simple metabolic requirements, which could allow a simple resource recycling of NH4⁺ and CO2 into a valuable product. The modality of hydrogen production/supply to HOBs represents a challenge. In th...
Article
The market of biobased products obtainable via fermentation processes is steadily increasing over the past few years, driven by the need to create a decarbonized economy. To date, industrial fermentation (IF) employs either pure or mixed microbial cultures (MMC) whereby the type of the microbial catalysts and the used feedstock affect metabolic pat...
Article
Microbial electricity-driven anoxic ammonium removal could remove ammonium from wastewater without the presence of oxygen (aeration) using electricity. This study aims at unveiling the potential biologic pathways for the bioelectrochemical oxidation of ammonium to dinitrogen gas in an anaerobic bioelectrochemical system (BES). Known intermediate me...
Article
Full-text available
Cathodic biofilms have an important role in CO2 bio-reduction to carboxylic acids and biofuels in microbial electrosynthesis (MES) cells. However, robust and resilient electroactive biofilms for an efficient CO2 conversion are difficult to achieve. In this review, the fundamentals of cathodic biofilm formation, including energy conservation, electr...
Article
Electroactive microorganisms can exchange electrons with other cells or conductive interfaces in their extracellular environment. This property opens the way to a broad range of practical biotechnological applications, from manufacturing sustainable chemicals via electrosynthesis, to bioenergy, bioelectronics or improved, low-energy demanding waste...
Article
Increasing carbon footprint alters the carbon balance in nature, thereby worsening global climate change. The conversion of carbon dioxide (CO2) into value-added products through biological routes is the pathway of the future because of its ecological and sustainable character. The present study evaluated the conversion of CO2 into short-chain fatt...
Article
In the light of the spread of treated wastewater use for irrigation, trace heavy metals pose a great risk to humans and aquatic life. An integrated electrochemical-adsorption (i.e. EC-AD) hybrid system was investigated for the removal of iron (Fe), zinc (Zn), and copper (Cu) traces from synthetic wastewater mimicking industrial wastewater. Slag, a...
Article
Groundwater pollution and salinization have increased steadily over the years. As the balance between water demand and availability has reached a critical level in many world regions, a sustainable approach for the management (including recovery) of saline water resources has become essential. A 3-compartment cell configuration was tested for a new...
Article
Full-text available
Electro bioremediation is gaining interest as a sustainable treatment for contaminated groundwater. Nevertheless, the investigation is still at the laboratory level, and before their implementation is necessary to overcome important drawbacks. A prevalent issue is the high groundwater hardness that generates scale deposition on electrodes that irre...
Conference Paper
Groundwater constitutes one of the main sources of drinking water supplies, and its full exploitation is hindered by the progressive accumulation of nitrates mostly deriving from inefficient agricultural practices. Bioelectrochemical systems (BES) represent a promising option for the treatment of nitrate-contaminated groundwater. They combine the p...
Article
Full-text available
The present work assessed the alliance of microbial electrochemical technologies (METs) and fermentation in a two-step process for the electro-bioconversion of carbon dioxide (CO2) into elongated chemical building blocks. The electro bio-reduction of CO2 into acetic acid and ethanol (EtOH:HAc) at a 1-to-1 ratio is linked to a subsequent elongation...
Article
This review paper aims to identify the main sources of carbon dioxide (CO2) emissions from wastewater treatment plants (WWTPs) and highlights several technologies developed to capture CO2 in this milieu. CO2 is emitted in all the operational units of conventional WWTPs, and even after the disposal of treated effluents and sludges. CO2 emissions fro...
Article
Full-text available
New strategies in inoculation and operation of bioelectrochemical systems were used to advance in the steering of bio-electro carbon dioxide (CO2) recycling towards chemical compounds. First, the preparation of microbe coated biocathodes ex-situ with putative electroactive species with CO2 as sole carbon source was assayed. Second, CO2 feeding stra...
Article
Full-text available
Microbial electrosynthesis (MES) provides a sustainable route for the conversion of CO2 and electricity into acetate and other organics. The conversion of CO2 takes place at a biologically active cathode (‘biocathode’), which is typically separated from the anode by an ion exchange membrane. Since both charged and uncharged species participate in t...
Article
This work aimed to study the electrification of biotrickling filters by means of Microbial electrochemical technologies (MET) to develop an easy-to-assemble and easy-to-use MET for nitrogen removal without external aeration nor addition of chemicals. Four different designs were tested. The highest ammonium and nitrate removal rates (94 gN·m-3·d-1 a...
Article
Full-text available
Microbial electrosynthesis (MES) for bioelectro carbon dioxide (CO 2 ) recycling is an interesting and sustainable opportunity to exploit off gases from industrial facilities and convert them into valuable energy sources....
Article
Full-text available
Decarbonisation of the economy has become a priority at the global level, and the resulting legislative pressure is pushing the chemical and energy industries away from fossil fuels. Microbial electrosynthesis (MES) has emerged as a promising technology to promote this transition, which will further benefit from the decreasing cost of renewable ene...
Article
Full-text available
The coexistence of different pollutants in groundwater is a common threat. Sustainable and resilient technologies are required for their treatment. The present study aims to evaluate microbial electrochemical technologies (METs) for treating groundwater contaminated with nitrate (NO3⁻) while containing arsenic (in form of arsenite (As(III)) as a co...
Book
Did you know that watching your favourite series on TV or just switching on your laptop for work, requires indirect water consumption? It's a proven fact that every time we use energy resources, water is also consumed. In the next few decades, global water and energy demands will increase significantly, but at this time there isn't a lot of informa...
Article
Full-text available
Renewable energies will represent an increasing share of the electricity supply, while flue and gasification-derived gases can be a promising CO2 feedstock with a heat load. In this study, microbial electrosynthesis of organic compounds from CO2 at high temperature was proposed as an alternative for valorising energy surplus and decarbonizing the e...
Article
Full-text available
Hydrogen is a key intermediate element in microbial electrosynthesis as a mediator of the reduction of carbon dioxide (CO2) into added value compounds. In the present work we aimed at studying the biological production of hydrogen in biocathodes operated at − 1.0 V vs. Ag/AgCl, using a highly comparable technology and CO2 as carbon feedstock. Ten b...
Presentation
C4-C6 organics production from CO2 through a two-step process by an anaerobic mixed culture. Study of each step optimal conditions
Article
Full-text available
Many industrial combustion processes produce carbon dioxide (CO 2) at high temperature, which may be electrically recycled into valuable chemicals using microorganisms as catalysts. However, little attention has been paid to handle the remaining heat of these processes as an alternative to increase CO 2 fixation and production rates. Thus, this stu...
Article
Full-text available
Lack of suitable electron donors or acceptors is in many cases the key reason for pollutants to persist in the environment. Externally supplementation of electron donors or acceptors is often difficult to control and/or involves chemical additions with limited lifespan, residue formation or other adverse side effects. Microbial electrochemistry has...
Article
Full-text available
Microbial conversion of waste gases, industrial off-gases, and CO2-rich streams is gaining momentum, with interest coming from the industrial users, governments giving support, and researchers working toward upscaling these systems. A multidisciplinary approach is needed to develop new metabolic pathways and to optimize existing processes. Syntheti...
Article
Climate change and future depletion of resources are two of the most important environmental challenges that humankind have ever faced. This mini-review aims to give a second chance to contaminated water and re-calcitrant carbon dioxide (CO 2) streams by putting forward a resilience and sustainable technology based on electron-driven microbial reac...
Article
Biogas production in wastewater treatment plants (WWTPs) plays a decisive role in the reduction of CO2 emissions and energy needs in the context of the water-energy nexus. The biogas obtained from sewage sludge digestion can be converted into biomethane by the use of biogas upgrading technologies. In this regard, an innovative water scrubbing based...
Article
Full-text available
Electromethanogenesis is the bioreduction of carbon dioxide (CO2) to methane (CH4) utilizing an electrode as electron donor. Some studies have reported the active participation of Methanobacterium sp. in electron capturing, although no conclusive results are available. In this study, we aimed at determining short-time changes in the expression leve...
Article
Reliable operational procedures for the control of Microbial Electrochemical Technologies (METs) to produce suitable substrates for economically viable downstream applications were investigated. We aimed at levelling key operational conditions in a model electrosynthetic reactor in order to obtain biofuels from carbon dioxide (CO2). The cathodes of...
Article
Full-text available
Revista: FuturEnviro. Abstract: El proyecto DigesTake investigará la valorización de los efluentes líquidos y gaseosos de la digestión anaerobia de lodos en depuradoras urbanas. El consorcio del proyecto, liderado por el Laboratorio de Ingeniería Química y Ambiental (LEQUIA) de la Universidad de Girona (UdG), integra diez universidades, empresas y...
Article
The rise of carbon dioxide (CO2) emissions and the accumulation of non-biodegradable plastics in the environment are leading to an environmental crisis. Thus, the bio-electro recycling of recalcitrant CO2 as feedstock to produce bioplastics could be an interesting solution to explore. In this work, a bioelectrochemical reactor was used to carry out...
Article
The high genetic similarity between some carboxydotrophic bacteria does not allow for the use of common sequencing techniques targeting the 16S rRNA gene for species identification. 16S rRNA sequencing fails to discriminate among Clostridium ljundahlii and 'Clostridium autoethanogenum', despite this two species exhibit significant differences in CO...
Article
Full-text available
This study aims at elucidating the metabolic pathways involved in the production of volatile fatty acids from CO2 and electricity. Two bioelectrochemical systems (BES) were fed with pure CO2 (cells A and B). The cathode potential was first poised at −574 mV vs. standard hydrogen electrode (SHE) and then at −756 mV vs. SHE in order to ensure the req...
Article
BACKGROUND Phosphorus (P) resources are limited, and to assure its supply, the coming actions must be focused on P‐recovery rather than its removal. In this sense, manure is a mining opportunity for nutrient recovery, for example, recovered as struvite. However, manure treatment is challenging due to its characteristics, such as the solid content,...
Article
Fresh water is a fundamental source for humans, hence the recent shrinkage in freshwater and increase in water pollution are imperative problems that vigorously affect the people and the environment worldwide. The breakneck industrialization contributes to the procreation of substantial abundance of wastewater and its treatment becomes highly indis...
Article
Biocathodic electrode-driven denitrification has been proved experimentally in complex biofilms. However, experimentation with isolated bacteria in pure culture is still limited. In this paper, six Alphaproteobacteria (Rhizobiales), found to be dominant in a denitrifying biocathode, have been characterized bioelectrochemically. Bacteria were isolat...
Article
Removal of nitrogen, mainly in form of ammonium (NH4+), in wastewater treatment plants (WWTPs) is a highly energy demanding process, mainly due to aeration. It causes costs of about half a million Euros per year in an average European WWTP. Alternative, more economical technologies for the removal of nitrogen compounds from wastewater are required....
Article
BACKGROUND This study provides a methodology for recovering two essential macronutrients (potassium and phosphorus) from the liquid fraction of manure. This methodology paves the way for K‐struvite recovery from manure by determining the suitable operational conditions (pH and temperature). RESULTS The theoretical operational conditions were estab...
Chapter
Formation of hydrogen, methane, and organics at biocathodes is an attractive new application of bioelectrochemical systems (BESs). Using mixed cultures, these products can be formed at certain cathode potentials using specific operating conditions, of which pH is important. Thermodynamically, the reduction of CO2 to methane is the most favorable re...
Article
Full-text available
Groundwater pollution is a serious worldwide concern. Aromatic compounds, chlorinated hydrocarbons, metals and nutrients among others can be widely found in different aquifers all over the world. However, there is a lack of sustainable technologies able to treat these kinds of compounds. Microbial electro-remediation, by the means of microbial elec...
Article
Full-text available
Large concentrations and oscillations of ammonium nitrogen (NH4+-N) in municipal landfill leachate pose considerable constraints to its further treatment in central wastewater treatment plants. The aim of this study was to evaluate and optimize two technologies for the pre-treatment of 600 L/day of landfill leachate: in particular, to optimize thei...
Poster
Full-text available
Bioelectrochemical production of methane and acetate from CO2 as the sole carbon source by a carboxydotrophic mixed culture and an enriched culture of the acetogen I-19 (100% similar to Eubacterium limosum and Butyribacterium methylotrophicum according to 16S rRNA gene similarity) respectively.
Article
Full-text available
Microbial fuel cells (MFCs) technology is a bio-approach to remove organic matter and nitrogen from wastewater with concomitant production of renewable electricity. Nowadays, it exists a clear interest in moving MFCs towards application. This study aimed to demonstrate MFCs technology feasibility treating swine manure. A couple of 6-stacked MFCs pr...
Article
MFC-based bioelectro-Fenton (BEF) system was examined in duplicate to deal with recalcitrant organics of mature landfill leachate pre-treated with partial nitritation-anammox process. The system performance was evaluated at various iron species (iron (II) sulfate and iron (III) chloride) and iron dosages (150, 300 and 500 mg L⁻¹) as Fenton catalyst...
Article
Full-text available
Denitrifying bioelectrochemical systems (BES) allows safe nitrate treatment in waters with low organic carbon content without chemicals requirements and at competitive cost. However, the technology should move towards scaling-up by improving removal rate capabilities. In this study, a novel tubular design was used to evaluate whether the hydraulic...
Article
To date acetate is the main product of microbial electrosynthesis (MES) from carbon dioxide (CO2). In this work a tubular bioelectrochemical system was used to carry out MES and enhance butyrate production over the other organic products. Batch tests were performed at a fixed cathode potential of − 0.8 V vs SHE. The reproducibility of the results a...
Article
Abstract Recently, microbial fuel cells (MFCs) have been integrated with membrane bioreactors (MBRs), either in an internal or an external configuration, for wastewater treatment and energy recovery. In an external configuration, MFCs could be applied for an efficient sludge reduction since they can simultaneously address energy issue and environme...
Article
Full-text available
The conversion of electrical current into methane (electromethanogenesis) by microbes represents one of the most promising applications of bioelectrochemical systems (BES). Electromethanogenesis provides a novel approach to waste treatment, carbon dioxide fixation and renewable energy storage into a chemically stable compound, such as methane. This...
Article
The growing development of technologies and processes for resource treatment and recovery is offering endless possibilities for creating new plant-wide configurations or modifying existing ones. However, the configurations’ complexity, the interrelation between technologies and the influent characteristics turn decision-making into a complex or uno...