Sean F. Lee

Sean F. Lee
  • Master of Science
  • PhD Student at Tulane University

About

5
Publications
948
Reads
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24
Citations
Current institution
Tulane University
Current position
  • PhD Student

Publications

Publications (5)
Article
Full-text available
Seedling recruitment is an important mode of establishment utilized by many invasive plants. In widespread invasive plants, regional variation in the rates of seedling recruitment can contribute to differences in invasion intensity across regions. In this study, we examined regional variation in reproductive traits and seedling performance in a cos...
Article
Full-text available
Escape from native range enemies can give invasive species a competitive edge according to the enemy‐release hypothesis. While more commonly associated with predators and herbivores, release from belowground microbial antagonists has been recently demonstrated to benefit invasive plants. Biogeographic variation in dominance and comparisons of soil...
Preprint
Full-text available
Seedling recruitment is an important mode of spread utilized by many invasive plants. In widespread invasive plants, regional variation in the rates of seedling recruitment can contribute to differences in invasion intensity across regions. In this study we examined regional variation in reproductive traits and seedling performance in a cosmopolita...
Preprint
Full-text available
Plants can cultivate soil microbial communities that affect subsequent plant growth through a plant-soil feedback (PSF). Strong evidence indicates that PSFs can mediate the invasive success of exotic upland plants, but many of the most invasive plants occur in wetlands. In North America, the rapid spread of European Phragmites australis cannot be a...
Article
Full-text available
Invasive plants often successfully occupy large areas encompassing broad environmental gradients in their invaded range, yet how invader dominance and effects on ecological communities vary across the landscape has rarely been explored. Furthermore, while the impacts of invasion on plant communities are well studied, it is not well understood wheth...

Questions

Question (1)
Question
Hi all,
I am trying to extract RNA from snap frozen leaf tissue ~100-150mg. I am getting good yields >150ng/uL normally with 260/280 >2.1 and 260/280 >2 as well. This is a good sign, but my gels are running smeared with some faint bands.
Our -80C tripped a breaker a few weeks back and the freezer was off for ~1.5 days. When discovered the freezer was still frozen (i.e. still had ice in it). however when we turned back on the freezer it was reading 1C.
I fear that this event may have lead to the degradation of the RNA. I attached pictures of the gels below. Thoughts?

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