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Savas G. Anastassiadis

Savas G. Anastassiadis
Pythia Institute of Biotechnology · Biotechnology, Industrial Microbiology, Bioengineering, Agrobusinesss, soil conditioners, biofuels, carbon resources

Diploma (Master), Dr. Nat. Sc. (Ph.D.), Ass. Prof., Visitor Prof., Owner of Research Center

About

55
Publications
50,948
Reads
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925
Citations
Citations since 2016
6 Research Items
397 Citations
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Introduction
I financed my studies in Germany and partly in USA myself, working nights as a musician (keyboards). I obtained my M.S. degree (Diploma) from the University of Münster (Germany) under the supervision of Professor Dr. H.-J. Rehm (One of the pioneers in biotechnology and fathers of Industrial Microbiology). Subsequently, I undertook my doctoral research under the joint supervision of Professor Dr. H.-J. Rehm of University of Münster and of Professor Dr. Christian Wandrey (One of the fathers of modern biotechnology) at the Biotechnology Institute within the Research Center Jülich (RCJ) in Germany (formerly known as Nuclear Research Center, KFA). My Ph.D. thesis was financed by Haarmann & Reimer Co., a daughter company of Bayer Co. (Leverkusen, Germany). Following the completion of my Ph.D., I worked for two and a half years as Postdoctoral Research Associate at the Metabolic Engineering group of Bayer Professor Dr. Gregory Stephanopoulos at the Chemical Engineering Department of M.I.T. and later on contract basis for an agency (USA). I was working as consultant and senior researcher at Thermo Fibergen Inc., a Thermo Electron company (1 patent application) and at Cargill Co., and as Research Associate at Tufts University (USA). I was serving in the Greek Army (Navy Hospital of Salamina) for about 6 months (starting in January 2000). Since October 2000, I am teaching Biotechnology, Ecology, Microbial Ecology, Microbiology and Biology (Assistant Professor) at the Environmental Engineering of Democritus University of Thrace (Xanthi, Greece). I carried out consulting for the ministry of development of Greek Government during the Greek Presidency for European Community. I have started a private international Research Center in Greece, named Pythia Institute of Biotechnology, which has an affiliation and cooperation (Research and Development and Publishing) with the the ddepartment of Microbiology (I am visitor professor) of the Russian Academy of Sciences.
Additional affiliations
January 2003 - December 2006
Greek Goverment
Position
  • Grant for innovative technologies (patents), 44000 Euro
Description
  • Greek General Secretary of Research and Technology (ΓΓΕΤ)
January 2003 - February 2015
Democritus University of Thrace
Position
  • Owner
January 2003 - November 2015
Pythia Institute of Biotechnology
Position
  • Research Director, Owner
Education
January 2005
Moscow Region
Field of study
  • Cooperation and affiliation with the Institute of Biochemistry and Physiology of Microorganisms and with the Institute of theoretical and experimental biophysics of the Russian Academy of Sciences (Publishing, Research and Development, Visitor Professor)
October 1998
Massachusetts
Field of study
  • Postdoctoral Research Associate, Tufts University
September 1996
Minessota. Massachusetts
Field of study
  • Senior Scientist (Cargill, ThermoElectron) (Biotechnology Industry)

Publications

Publications (55)
Article
Full-text available
Since early human history and existence energy rich plants, wood and forest cellulosic material have been used for fire, light, heating, cooking and other daily activities. Fossil energy was the foundation of our modern society and industrialization since last two centuries, while exploration and exploitation of oil reserves and petrochemistry have...
Article
Full-text available
Carbon atom is most important and abundant constituent of existing and new generated biological mater and biomass and the basis of all forms of life on earth. It is involved in the composition and construction of organic micro- and macromolecules, cells and living organisms, storage molecules, fossils, fossil fuels, biofuels and energy resources of...
Article
Full-text available
Carbon atom Carbon atom is most important and abundant constituent of existing and new generated biological mater and biomass and the basis of all forms of life on earth. It is involved in the composition and construction of organic micro- and macromolecules, cells and living organisms, storage molecules, fossils, fossil fuels, biofuels and energy...
Article
Full-text available
The possibility of using glycerol and glycerol-containing waste from biodiesel manu-facture as a carbon and energy source for microbiological production of citric acid has been studied. Acid formation on the selective media had previously been tested in 66 yeast strains of different genera (Candida, Pichia, Saccharomyces, Torulopsis and Yarrowia)....
Article
Full-text available
The possibility of using glycerol and glycerol-containing waste from biodiesel manufacture as a carbon and energy source for microbiological production of citric acid has been studied. Acid formation on the selective media had previously been tested in 66 yeast strains of different genera (Candida, Pichia, Saccharomyces, Torulopsis and Yarrowia). U...
Chapter
In the present work, the possibility of using glycerol and glycerol-containing waste from biodiesel manufacture as a carbon and energy source for microbiological production of citric acid was studied. Acid-formation ability on selective media was preliminary tested for 66 yeast strains of different genera (Candida, Pichia, Saccharomyces, Torulopsis...
Article
Full-text available
New processes have been previously described for the continuous and discontinuous production of gluconic acid by Aureobasidium pullulans (de bary) Arnaud. Little is known about the regulatory mechanisms of gluconic acid production by A. pullulans. The response of growth and gluconic acid metabolism to a variable profile of iron concentra-tions was...
Article
Full-text available
Current Review article summarizes the developments in citric acid production technologies in East and West last 100 years. Citric acid is commercially produced by large scale fermentation mostly using selected fungal or yeast strains in aerobe bioreactors and still remains one of the runners in industrial production of biotechnological bulk metabol...
Article
Current Review article summarizes the developments in citric acid production technologies in East and West last 100 years. Citric acid is commercially produced by large scale fermentation mostly using selected fungal or yeast strains in aerobe bioreactors and still remains one of the runners in industrial production of biotechnological bulk metabol...
Conference Paper
Poster and oral presentation entitled: Production of a novel antifreezing, growth and production promoting and disease protecting fertilizer for plants and trees (www.pythia-biotechnology.com, ecoplant.bg) Russian Scientists, Russian Academy of Sciences
Conference Paper
Organization and Oral presentation EcoPlant - Novel superior antifreeze soil conditioner Scientists from Russian Academy of Sciences
Conference Paper
Invitation from Directorate of EU (president of biotechnology) and the STATE UNIVERSITY – HIGHER SCHOOL OF ECONOMICS, MOSCOW, RUSSIA.
Article
Amino acids are the basic bioelements of proteins, which are the most important macromolecules for the functions of humans and animals. Out of the 20 L-amino acids, ecumenically found in most of living organisms, L-lysine is one of the 9 amino acids which are essential for human and animal nutrition. L-lysine is useful as medicament, chemical agent...
Article
Full-text available
Gluconic acid, the oxidation product of glucose, is a mild neither caustic nor corrosive, non toxic and readily biodegradable organic acid of great interest for many applications. As a multifunctional carbonic acid belonging to the bulk chemicals and due to its physiological and chemical characteristics, gluconic acid itself, its salts (e.g. alkali...
Article
Full-text available
Amino acids are the basic bioelements of proteins, which are the most important macromolecules for the functions of humans and animals. Out of the 20 L-amino acids, ecumenically found in most of living organisms, L-lysine is one of the 9 amino acids which are essential for human and animal nutrition. L-lysine is useful as medicament, chemical agent...
Article
Full-text available
Little is known about the regulatory mechanisms for citric acid production by different yeast. It is suggested the important role of iron ions in this process; iron is the integral component of many metalloenzymes; such as aconitate-hydratase, catalase, peroxidases and components of mitochondrial electron transfer chain. The response of citric acid...
Article
Gluconic acid, the oxidation product of glucose, is a mild neither caustic nor corrosive, non toxic and readily biodegradable organic acid of great interest for many applications. As a multifunctional carbonic acid belonging to the bulk chemicals and due to its physiological and chemical characteristics, gluconic acid itself, its salts (e.g. alkali...
Article
The application of a new developed process for the continuous production of gluconic acid using a cascade of two bioreactors in a continuous process is shown reaching the highest concentration of gluconic acid described in the literature for continuous culture fermentation. Very high gluconic acid concentrations of 272–308 g/l have been achieved un...
Article
Full-text available
New alternative processes for the continuous production of gluconic acid by Aureobasidium pullulans , using biomass retention by cell immobilization or cross over filtration, are described in the present work. 315 g/l gluconic acid was continuously produced in chemostat cultures at 21 hrs residence time without any biomass retention. 260 g/l glucon...
Article
BIP- Article New alternative processes for the continuous production of gluconic acid by Aureobasidium pullulans, using biomass retention by cell immobilization or cross over filtration, are described in the present work. 315 g/l gluconic acid was continuously produced in chemostat cultures at 21 hrs residence time without any biomass retention. 2...
Article
Full-text available
BIP Article The influence of air saturation and temperature on the continuous citric acid secretion was studied in chemostat cultures of Candida oleophila ATCC 20177 (var.). Simultaneous measurements of intra- and extracellular concentration of glucose, citric and isocitric acid confirmed the involvement of a specific active transport system in cit...
Article
Full-text available
The influence of air saturation and temperature on continuous citric acid secretion was studied in chemostat cultures of Candida oleophila ATCC 20177 (var.). Simultaneous measurements of intra- and extracellular concentration of glucose, citric and isocitric acid confirmed the involvement of a specific active transport system in citrate secretion,...
Article
The influence of air saturation and temperature on continuous citric acid secretion was studied in chemostat cultures of Candida oleophila ATCC 20177 (var.). Simultaneous measurements of intra- and extracellular concentration of glucose, citric and isocitric acid confirmed the involvement of a specific active transport system in citrate secretion,...
Article
Full-text available
BIP Article Present manuscript presents continuous processes using yeasts as alternatives for an industrial production of citric acid. The effect of air saturation on citric acid production by Candida oleophila in batch, repeated batch and chemostat cultures has been studied. In contrary to continuous fermentation (chemostat mode) displaying an op...
Article
Full-text available
The effect of air saturation on citric acid production in batch, repeated batch and chemostat cultures has been studied. It was shown that, under continuous fermentation (chemostat mode), the highest concentration of citric acid equal of 98 g/l was produced at 20% of air saturation. In contrary to continuous fermentation, displaying an optimum at 2...
Article
A team of researchers investigated the influence of pH on continuous citric acid secretion by the yeast Candida oleophila ATCC 20177. Continuous fermentation experiments under ammonium ion-limiting steady state conditions were carried out using glucose as a carbon source. Results show that active transport system for citrate excretion is the main s...
Article
This study was focused on the optimization of a new fermentation process for continuous gluconic acid production by the isolated yeast-like strain Aureobasidium pullulans DSM 7085 (isolate 70). Operational fermentation parameters were optimized in chemostat cultures, using a defined glucose medium. Different optima were found for growth and gluconi...
Article
Full-text available
The pH influence on continuous citric acid secretion was investigated in Candida oleophila ATCC 20177 (var.) under NH4+ limiting state steady conditions, using glucose. Highest citric acid concentration of 57.8 g/l, citrate/isocitrate ratio of 15.6, space-time yield of 0.96 g/(l x hr) and biomass specific productivity of 0.041 g/(g x hr) were obtai...
Article
Full-text available
The pH influence on continuous citric acid secretion was investigated in Candida oleophila ATCC 20177 (var.) under NH4+ limiting state steady conditions, using glucose. Highest citric acid concentration of 57.8 g/l, citrate/isocitrate ratio of 15.6, space-time yield of 0.96 g/(l x hr) and biomass specific productivity of 0.041 g/(g x hr) were obtai...
Article
The central aspect of this work was to investigate the influence of nitrogen feed rate at constant C/N ratio on continuous citric acid fermentation by Candida oleophila ATCC 20177. Medium ammonia nitrogen and glucose concentrations influenced growth and production. Space-time yield (STY) meaning volumetric productivity, biomass specific productivit...
Article
By extensive microbial screening, about 50 strains with the ability to secrete gluconic acid were isolated from wild flowers. The strains belong to the yeast-like mould Aureobasidium pullulans (de Bary) Arnaud. In shake flask experiments, gluconic acid concentrations between 23 and 140 g/l were produced within 2 days using a mineral medium. In batc...
Article
A suitable strain and important factors influencing citric acid formation in yeasts were identified. Candida oleophila ATCC 20177 was chosen as the best citric acid producer from several Candida strains. Yields of 50 g/l citric acid were produced in shake flask and 80 g/l in fed-batch fermentations with 1.5 and 3 g/l NH(4)Cl under non-optimized con...
Chapter
Full-text available
Anastassiadis S. (1993). Institute of Biotechnology 2, Research Center Jülich (KFA) in Germany. "; "Determination of organic acids, especially citric and isocitric acid, in fermentation solutions and fruit juices"
Research Proposal
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