Satoshi Konishi

Satoshi Konishi
Duke Univeristy · Tata lab (the Department of Cell Biology)

MD,PhD

About

29
Publications
3,036
Reads
How we measure 'reads'
A 'read' is counted each time someone views a publication summary (such as the title, abstract, and list of authors), clicks on a figure, or views or downloads the full-text. Learn more
547
Citations
Additional affiliations
January 2020 - present
Duke University
Position
  • PostDoc Position
September 2015 - present
Osaka University
Position
  • PostDoc Position
April 2012 - March 2016
Kyoto University
Position
  • postgrad student
Education
April 2001 - March 2007
Kyoto University
Field of study
  • Medicine

Publications

Publications (29)
Article
Full-text available
Multi-ciliated airway cells (MCACs) play a role in mucociliary clearance of the lung. However, the efficient induction of functional MCACs from human pluripotent stem cells has not yet been reported. Using carboxypeptidase M (CPM) as a surface marker of NKX2-1(+)-ventralized anterior foregut endoderm cells (VAFECs), we report a three-dimensional di...
Article
Full-text available
Objective The goals of this study were to assess the efficacy and tolerability of pirfenidone in patients with idiopathic pulmonary fibrosis (IPF) and to identify predictors of tolerability to pirfenidone. Methods We conducted a retrospective observational study. When the patient showed deterioration in the percent forced vital capacity (%FVC) or e...
Article
Full-text available
The discriminative power of CURB-65 for mortality in community-acquired pneumonia (CAP) is suspected to decrease with age. However, a useful prognostic prediction model for older patients with CAP has not been established. This study aimed to develop and validate a new scoring system for predicting mortality in older patients with CAP. We recruited...
Article
Mucociliary clearance is an essential lung function that facilitates the removal of inhaled pathogens and foreign matter unidirectionally from the airway tract and is innately achieved by coordinated ciliary beating of multicili-ated cells. Should ciliary function become disturbed, mucus can accumulate in the airway causing subsequent obstruction a...
Article
Full-text available
Multiciliated cells (MCCs) in tracheas generate mucociliary clearance through coordinated ciliary beating. Apical microtubules (MTs) play a crucial role in this process by organizing the planar cell polarity (PCP)-dependent orientation of ciliary basal bodies (BBs), for which the underlying molecular basis remains elusive. Herein, we found that the...
Article
A 78-year-old man presented with hypercalcemia and renal disease with high serum IgG4 and positive myeloperoxidase anti-neutrophil cytoplasmic antibody (MPO-ANCA), exhibiting sarcoidosis-like chest findings. A renal biopsy revealed tubulointerstitial nephritis, membranous nephropathy (MN), and sub-capsular lymphoid aggregates without fulfilling the...
Article
Introduction Risk factors associated with the new detection of methicillin-resistant Staphylococcus aureus (MRSA) during hospitalization remain unclear. This study aimed to identify risk factors associated with MRSA isolation from the sputum of patients admitted with pneumonia, during their hospitalization. Methods Patients were prospectively enro...
Article
Full-text available
Background: Ceftriaxone (CTRX) and ampicillin/sulbactam (ABPC/SBT) are recommended by various guidelines as the first-line antibiotics for community-acquired pneumonia (CAP). However, which of these antibiotics is more effective for treating non-aspiration CAP remains unclear. Methods: This study was a prospective, single-center, open-label, qua...
Poster
Full-text available
To model the alignment and orientation of cilia we first model the Self-Organization process of the Microtubule (MT) bundles. Multiciliated Cells (MCCs)’ cilia tend to align and orient in a series of parallel stripes, passing first through several stages. Real cells have an irregular shape, approximately hexagonal. Asymmetric time-dependent cues ar...
Article
Full-text available
The paracellular barrier function of tight junctions (TJs) in epithelial cell sheets is robustly maintained against mechanical fluctuations, by molecular mechanisms that are poorly understood. Vinculin is an adaptor of a mechanosensory complex at the adherens junction. Here, we generated vinculin KO Eph4 epithelial cells and analyzed their confluen...
Article
Background: Treatment of aspiration pneumonia is an important problem due to aging of populations worldwide. However, the effectiveness of cefepime in aspiration pneumonia has not yet been evaluated. Aim: To compare the clinical efficacy and safety of cefepime and meropenem in patients with moderate-to-severe aspiration pneumonia. Methods: In...
Article
Full-text available
It has been challenging to generate in vitro models of alveolar lung diseases, as the stable culture of alveolar type 2 (AT2) cells has been difficult. Methods of generating and expanding AT2 cells derived from induced pluripotent stem cells (iPSCs) have been established and are expected to be applicable to disease modeling. Hermansky-Pudlak syndro...
Conference Paper
Full-text available
Multiciliated Cells’ (MCCs) cytoskeleton, in particular microtubules, tend to align in a series of grids and stripes, passing through several stages. MCC’s ciliar basal bodies (BBs) tend position themselves following the alignment of the cytoskeleton. Moreover, the BBs’ tend to orient their basal feet (BF) towards the cytoskeleton bundles at an ang...
Poster
Full-text available
MCCs cytoskeleton tend to align in a series of stripes, passing through several stages. Multiciliated Cells (MCCs)'s basal bodies (BBs) tend follow the alignment of the cytoskeleton. The BBs' tend to orient their basal feet (BF) towards the cytoskeleton at an angle. In order to model the alignment and orientation of BBs we first model the alignment...
Article
Full-text available
The stable expansion of tissue-specific stem cells in vitro has contributed to research on several organs. Alveolar epithelial type II (AT2) cells function as tissue stem cells in the lung, but robust models for studying human AT2 cells are lacking. Here we report a method for the efficient generation and long-term expansion of alveolar organoids (...
Article
Full-text available
Background In the past decade, extended-spectrum β-lactamase (ESBL)-producing bacteria have increasingly frequently been isolated from various kinds of clinical specimens. However, the appropriate treatment of pneumonia in which ESBL-producing bacteria are isolated from sputum culture is poorly understood. Purpose To investigate whether or not ESBL...
Article
Full-text available
No methods for isolating induced alveolar epithelial progenitor cells (AEPCs) from human embryonic stem cells (hESCs) and induced pluripotent stem cells (hiPSCs) have been reported. Based on a study of the stepwise induction of alveolar epithelial cells (AECs), we identified carboxypeptidase M (CPM) as a surface marker of NKX2-1(+) "ventralized" an...
Article
We herein report the clinical and laboratory characteristics of two anti-OJ (anti-isoleucyl-tRNA synthetase) autoantibody-positive interstitial lung disease patients with polymyositis/dermatomyositis (PM/DM). We compared these characteristics with previously published findings. Previous reports and our present cases show that anti-OJ autoantibody-p...
Article
Hypereosinophilic syndrome (HES) encompasses both myeloproliferative and lymphoproliferative diseases. We encountered a rare case of lymphocytic HES followed by malignant T cell lymphoma, who was diagnosed as eosinophilic pneumonia upon the first visit. During the clinical course, the transition of the chest CT findings from bilateral multifocal gr...
Article
Legionella pneumonia tends to be severe and is known to be fatal. Introduction of the urinary Legionella antigen test and changes in the Infectious Disease Law have led to increased numbers of reports, and milder cases are now occasionally seen. We experienced three cases demonstrating mild respiratory infections and one case demonstrating nosocomi...
Article
A 50-year-old man with a 30-year occupational history of welding presented with low-grade fever, fatigue and persistent dry cough. Computed tomography (CT) of the chest revealed interlobular septal thickening and bilateral non-segmental patchy ground-glass opacities except in the sub-pleural zone. He revealed that he had inhaled nickel fumes 3 days...

Network

Cited By

Projects

Projects (2)
Project
Considering Multi-Ciliated Cells (MCCs) we develop mathematical modeling towards the goal of understanding and predicting the alignment and orientation mechanisms of the cilia which could potentially lead to better understanding of some ailments such as asthma.