Satish Verulkar

Satish Verulkar
Indira Gandhi Agricultural University · Department of Plant Molecular Biology and Biotechnology

Ph.D.

About

125
Publications
35,780
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2,111
Citations
Citations since 2017
42 Research Items
1417 Citations
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2017201820192020202120222023050100150200250300
2017201820192020202120222023050100150200250300
2017201820192020202120222023050100150200250300

Publications

Publications (125)
Article
Arsenic (As) and cadmium (Cd), two major carcinogenic heavy metals, enters into human food chain by the consumption of rice or rice-based food products. Both As and Cd disturb plant-nutrient homeostasis and hence, reduces plant growth and crop productivity. In the present study, As/Cd modulated responses were studied in non-basmati (IR-64) and basm...
Article
Rice is a major cereal crop which is negatively impacted by soil-salinity, both in terms of plant growth as well as productivity. Salinity tolerant rice varieties have been developed using conventional breeding approaches, however there has been limited success which is primarily due to the complexity of the trait, low yield, variable salt stress r...
Preprint
Full-text available
Direct selection for yield under drought has resulted in the release of a number of drought-tolerant rice varieties across Asia. In this study, we characterized the physiological traits that have been affected by this strategy in breeding trials across sites in Bangladesh, India, and Nepal. Drought-breeding lines and drought-tolerant varieties show...
Article
Full-text available
Direct selection for yield under drought has resulted in the release of a number of drought-tolerant rice varieties across Asia. In this study, we characterized physiological traits affected by that strategy in breeding trials across sites in Bangladesh, India, and Nepal. The drought breeding lines and drought-tolerant varieties showed consistently...
Article
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The complexity of genotype × environment interactions under drought reduces heritability, which determines the effectiveness of selection for drought tolerance and development of drought tolerant varieties. Genetic progress measured through changes in yield performance over time is important in determining the efficiency of breeding programmes in w...
Article
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Rice is a primary staple food for more than half of the world’s population and more that 70% of Indian population. Chhattisgarh state is one of the major parts of India having richest rice biodiversity with wide genetic diversity. To make advances in rice molecular design breeding, it is important to understand the genetic background and phenotypes...
Article
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Rice is the most important food crop both in value and volume for the Asian population. Frequent drought, flood and salinity stresses exacerbated by global climate change adversely affect rice production in more than fifty percent of the rice growing areas. Green revolution high yielding varieties carrying sd1 dwarfing gene have almost fully replac...
Article
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18. Expression profiling of metal homeostasis related candidate genes in rice (Oryza spp.) using semi quantitative RT-PCR analysis.
Article
Full-text available
Background: Climate extremes such as drought and flood have become major constraints to the sustainable rice crop productivity in rainfed environments. Availability of suitable climate-resilient varieties could help farmers to reduce the grain yield losses resulting from the climatic extremities. The present study was undertaken with an aim to dev...
Article
Full-text available
Key message The study provides high-throughput protocol for ploidy determination which may accelerate the production of double haploids in rice. Abstract Advancements in anther-culture technique have accelerated the production of completely homozygous breeding lines in a shortened time frame. However, the success is dependent upon several factors...
Article
Full-text available
Nine hundred and thirty seven (788 HvSSR, 108 RM, 5 RGNMS and 36 SNP) markers were surveyed for parental polymorphism between indica cultivars Danteshwari and Dagad Deshi. One hundred and four (11.9 %) markers exhibited polymorphisms which were further analyzed for marker segregation on F7 275 RILs. Thirty eight (36.54%) markers showed expected 1:1...
Article
Full-text available
Flooding is one of the major constraints for rice production in rainfed lowlands, especially in years and areas of high rainfall. Incorporating the Sub1 (Submergence1) gene into high yielding popular varieties has proven to be the most feasible approach to sustain rice production in submergence-prone areas. Introgression of this QTL into popular va...
Article
Full-text available
Sheath blight, caused by the pathogen Rhizoctonia solani Kühn, is one of the most serious diseases of rice and leads to severe yield losses worldwide. A recombinant inbred line (RIL) population consisting of 122 lines was constructed from a cross between Danteshwari a high yielding popular rice cultivar but moderately susceptible to water stress, s...
Research
Marker assisted selection was employed to pyramid three bacterial blight resistance genes Xa21, xa13 and xa5 into high yielding susceptible local varieties Dubraj and Safri-17. Homozygous and heterozygous genotypes were identified in Dubraj X RP-Bio-226 and Safri17 X RP-Bio-226 crosses with the help of PCR markers and goodness of fit was tested. We...
Poster
Full-text available
The advancement in anther-culture technique has accelerated the production of completely homozygous breeding lines in a shortened time frame. The success is dependent upon precise and accurate determination of ploidy status at callus stage. Present study focuses on an optimized high throughput flow-cytometry methodology for quick ploidy analysis in...
Article
Full-text available
AimsDrought is the major constraint to rainfed rice productivity in South Asia, but few reports provide detailed characterization of the soil properties related to drought stress severity in the region. The aim of the study was to provide a compilation of drought breeding network sites and their respective levels of drought stress, and to relate so...
Article
Phosphorus (P) deficiency is a major constraint to rice production worldwide. Especially developing countries like India, having a limited access to P fertilizer. Genetic variations in rice in terms of root length, density of root hairs, modified root architecture, have been observed in low P concentration. Adaptive root modifications enhance P acq...
Article
Full-text available
Phosphorus (P) deficiency is a major constraint to rice production worldwide. Especially developing countries like India, having a limited access to P fertilizer. Genetic variations in rice in terms of root length, density of root hairs, modified root architecture, have been observed in low P concentration. Adaptive root modifications enhance P acq...
Article
Full-text available
Nitrogen use efficiency (NUE) in the rice field is approximately 33%, poorest among cereals and a substantial proportion of the remaining 67% is lost into the environment, reducing economic efficiency of applied N. This calls for immediate development of comprehensive approach to optimize N management. Moreover, plant-useable N is consumed as nitra...
Article
Full-text available
To meet the future food demands rice varieties with increased yield potential have to be developed for sustainable crop production across wide range of environments and over the years. Therefore, present research aimed to estimate the genetic variability and path analysis with eighty five lines of mapping population derived from cross between Swarn...
Article
Full-text available
With the substantial increase in food grain production, much of the emphasis has been put for enhanced grain quality traits. Most of the grain qualities traits are polygenic in nature. Identification of QTLs and markers linked to these QTLs can substantially enhance the selection efficiency. In this study, an effort has been made to identify QTLs f...
Article
Full-text available
DNA barcoding is a technique that makes use of short sequences from a standardized region of a genome to provide quick and reliable identification of species among all forms of life. The presence of uniqueness and variability required for DNA barcoding is well reported in animal system based on mitochondrial gene CO1.On the other hand, limited info...
Article
Full-text available
Seventy one rice genotypes were studied for genetic variability, correlation and path analysis under irrigated and rainfed condition. The PCV values were greater than GCV, revealing little influence of environment in character expression. Analysis of variance was found to be significant for most of the traits, indicating that there is existence of...
Article
In this study characterization of hundred landraces/farmers varieties of rice (Safri) collected from different part of chhattishgarh was performed using DUS testing protocol. Characterization was done using forty six agro-morphological traits following Distinctiveness, Uniformity and Stability test (DUS) during kharif season of 2014 at the biodiver...
Article
Full-text available
An attempt was made to carry out in silico characterization of 14 putative QTL regions identified on chromosomes 1, 2, 4, 5, 6 and 8 associated with drought tolerance. A total of 354 BAC/PAC clones were detected in the underlying regions of putative QTLs mapped. These BAC/PAC clone nucleotide sequences were used for mining SSRs and primer designing...
Article
Bacterial leaf blight (BLB) of rice is caused by Xanthomonas oryzae pv. oryzae (Xoo), this is a major disease of rice in several countries. The present investigation was undertaken with the objective to develop high yielding rice varieties possessing broad spectrum durable resistance by transferring bacterial leaf blight (BLB) resistant genes viz.,...
Article
Full-text available
Phosphorus (P) deficiency is a major constraint to rice production worldwide. Especially developing countries like India, having a limited access to P fertilizer. Genetic variations in rice in terms of root length, density of root hairs, modified root architecture, have been observed in low P concentration. Adaptive root modifications enhance P acq...
Article
Full-text available
Global rice production in 2015-16 is forecast, a marginal fall from previous year, the projected trade is maintained at same level (42 million tons). The consumption likely to increase by 1%, the global carry over stock at end of 2015-16 is anticipated decrease by 12%. Although conventional breeding techniques have considerably increased the produc...
Article
Full-text available
Rice is a staple cereal of India cultivated in about 43.5 Mha area but with relatively low average productivity. Abiotic factors like drought, flood and salinity affect rice production adversely in more than 50% of this area. Breeding rice varieties with inbuilt tolerance to these stresses offers an economically viable and sustainable option to imp...
Research
Full-text available
From QTL to variety- harnessing the benefits of QTLs for drought, flood and salt tolerance in mega rice varieties of India through a multi-institutional network
Article
Full-text available
In present study, quantitative trait loci (QTL) analysis was carried out using 93 SSR and 11 SNP markers in F7 recombinant inbred lines derived from a cross between two potential indica genotypes, Danteshwari and Dagad Deshi. Fourteen putative QTLs identified for 7 root and shoot traits on chromosomes 1, 2, 4, 5, 6 and 8, explained 3.77%-29.24% phe...
Article
Full-text available
The present investigation for finding out genetic diversity at molecular level in 48 genotypes of grasspea using 24 ISSR markers. Out of 24 ISSR markers involved, 12 ISSR markers showed polymorphism which were used for diversity analysis. The selected genotypes were distributed into two major clusters having 0.61 similarity coefficient. The similar...
Article
The present investigation was undertaken to assess the genetic diversity of flax cultivars using molecular markers. 90 SSR and 10 ISSR flax markers were employed for molecular diversity analysis. Out of these, 25 microsatellite markers were found to be polymorphic giving a total of 99 bands with an average of 3.96 bands per marker ranging from 2 to...
Article
For testing seed purity and trueness, GOT (Grow Out Test) is a long term process and therefore, present study was carried out during Rabi-2011. The experimental materials used in the study comprised of four parents viz., Green Long (GL), Pusa Purple long (PPL), IBWL-2007-1 (IBWL), Punjab Sadabahar (PS) and their six F1’s and standard check PH-6. RA...
Article
Full-text available
Molecular markers are useful tools for determining cultivar identity. The purpose of this study to establish fingerprints of four rice varieties in Chhattisgarh. Microsatellite combines several features of an ultimate molecular marker and they are being used increasingly in various plant genetic studies and applications. The varieties were evaluate...
Article
Five lines of four mungbean genotypes were analyzed for polymorphism using a total of five Inter Simple Sequence Repeat (ISSR) and two Simple Sequence Repeat (SSR) markers. Unique alleles differentiating the genotypes could be identified. Amplification of genomic DNA of most popular four Indian mungbean genotypes with these ISSR and SSR primers yie...
Article
Full-text available
Drought is one of the most severe constraints reducing rice (Oryza sativa L.) yield in rainfed environments. ‘Sahbhagi Dhan’ (IR74371-70- 1-1) is a drought-tolerant rice variety that was released in India in 2010—and subsequently in Nepal as ‘Sukha Dhan 3’ and in Bangladesh as ‘BRRI Dhan 56’—and has performed well in rainfed farmers’ fields. This s...
Conference Paper
Full-text available
DNA based microsatellite and ISSR markers were used to establish profile of four rice (Oryza sativa L.) varieties, followed by identification of most informative markers. A total number of 160 alleles were obtained using 24 microsatellite primer pairs on four rice varieties. Six microsatellite markers (RM 247, RM 303, RM 164, RM 108, RM 72 and RM 1...
Article
Full-text available
The relationship between genetic diversity of parental lines with hybrid yield was examined to assess whether microsatellite markers are useful for evaluating germplasm and predicting F1yield potential and heterosis in rice. Among the five rice hybrids studied, KRH 2 showed maximum diversity between its parental lines, followed by Indira Sona, Sahy...
Article
Full-text available
The estimation of rice seed purity is conventionally done by a grow-out-test (GOT). As well as other issues, the time required for GOT is a major limitation. In this study, DNA-based microsatellite and ISSR markers were used to establish fingerprints of four rice varieties and used for assessment of genetic purity. A total of 160 alleles were obtai...
Article
A field experiment was conducted to compare and evaluate sixty different rice genotypes of F3 population grown under drought stress conditions. They were selected for estimation of drought susceptibility index (DSI) under three different water regimes. The use of DSI is likely to be most beneficial in selecting parents for development of drought re...
Article
A simple, rapid, and efficient method of leaf DNA extraction was optimized for PCR based Marker Assisted Selection (MAS) in rice. The procedure involved homogenization of leaves of 10-days old seedlings in the extraction buffer, followed by centrifugation. The supernatant was directly used for PCR. The isolated DNA had protein and RNA impurities. H...
Article
Drought tolerance in rice is a complex trait collectively determined by numerous component traits. These traits are governed by many genes with huge environmental interactions, with low heritability, and thus are difficult to investigate. In this context, it is important to define the drought tolerance from practical point of view and design a scre...
Article
Rice is a cold sensitive plant. During off-season cultivation this crop experiences cold at seedling stage. The present study was based on 122 RILs in F-13 generation developed by single-seed descent method from a cross between two indica cultivars Danteshwari and Dagad deshi. Screening was conducted during December 2012-January 2013 with seven rep...
Article
Fingerprinting with molecular markers allows precise and rapid variety identification. The present investigation was carried out with the following objectives: (i) to identify informative SSR marker allele(s) for fingerprinting popular flax cultivars cultivated in Chhattisgarh state of India, and (ii) to validate the utility of the genotype specifi...
Article
Rice is a major crop grown under all the conditions due to its adaptive nature. Present investigation of genetic diversity analysis was based on 46 selected unique germplasm (based on their morphological and biochemical properties) from IGKV, Raipur and 2 check varieties of rice. Diversity analysis was conducted using ISSR, microsatellite markers a...
Article
A RIL population (F12 and F13 generation) developed from a cross between the two indica cultivars, Danteshwari (high yielding variety) and Dagad deshi (drought tolerant local land race). The population was phenotyped under combination of absolute rainfed (RF), terminal stage drought (TSD) and irrigated (I) conditions with direct seeding and transpl...
Article
Full-text available
Significant variation in all the six yield and yield attributing traits were obtained. High variation was observed in grain yield and least variation was recorded in plant height. Among the 122 lines, line no. 12 exhibited highest grain yield under TSD condition. In correlation studies, grain yield exhibited significant and positive association wit...
Article
Full-text available
Microsatellite based analysis was carried out to access the genetic relatedness among eight genotypes of rice viz., IR 58025A, Pusa 6A, KMR 3R, BR 827-35-3-1-1-1R, R 710-437-1-1, PRR 78, Swarna and MTU-1010. Of the 45 microsatellite loci analyzed, only nine were identified as polymorphic among the various genotypes. The degree of similarity ranges...
Article
Full-text available
Four rice hybrids viz., KRH-2, Sahyadri, Indira Sona and Pusa RH-10 along with their parental lines were tested for genetic purity and their identity using microsatellite markers. Out of 53, eight microsatellite markers distributed on six chromosomes showed polymorphism between the parental lines of one or more hybrids. The highest frequency of pol...
Article
A field experiment was conducted at the Instructional cum Research Farm of IGKV, Raipur (C.G.), India during Kharif 2009 to study the relationship of different traits of rice (Oryza sativa L.) for drought tolerance under stress conditions. The materials used in the present study include 117 advanced breeding lines developed from different donors. T...
Article
Full-text available
The present study was conducted to characterize an Indira Sona hybrid from Chhattisgarh State. The morphological traits and grain quality characters studied were days to 50% flowering, days to maturity, tillering ability, panicle 1length, spikelets panicle , dehulled grain length, dehulled grain width, length/breadth, aroma, alkali value, amylose c...
Article
Full-text available
Variety identification has great significance from seed production, breeding as well as intellectual property rights point of view to ensure quality seed. Four varieties of rice were identified on the basis of seed colour (phenol, modified phenol and NaOH) and seedling response (GA3 and 2, 4-D) to chemical tests. Though no individual chemical test...
Article
Full-text available
Background: Rice (Oryza sativa L.) is a highly drought sensitive crop, and most semi dwarf rice varieties suffer severe yield losses from reproductive stage drought stress. The genetic complexity of drought tolerance has deterred the identification of agronomically relevant quantitative trait loci (QTL) that can be deployed to improve rice yield u...
Data
Performance of 84 IR64-NILs under non –stress (NS) and drought stress (S) conditions. (DOCX)