Sasha Nealand

University of Florida | UF

Where does this RV followed by a number come from?

I want to grow aerobic mycobacteria in 96 well plate without contamination for 3 days time. If I grow with the plastic cover on will it cut off their oxygen?

What information do the blue(positive contour) and green(negative contour) tell me in HSQC? Can I discover any information about a structure depending on if the contour is negative or positive?

was reading an older textbook (Duddeck) they describe H,C correlated COSY, interpreted as C connected to H. Is this essentially interpreted the same as HSQC and HETCOR?

I have to use a magnifying glass to read the display on a lot of the parts of the program, how can I change the fonts into something readable? I already tried changing Windows Settings->All in One Fonts & Icon Size to the large setting, it only changes some of the fonts.

Makes the program really hard to work with if I cant see any of the commands because they are too miniscule

Can anyone explain how to use a glass hygrometer to find the % water remaining in solvents distilled from the rotovap so that I can reuse them for chromatography? Where can I get the chart that will relate the numbers on the hygrometer to % water for common solvents such as methanol, hexane, ethyl acetate....

Thanks!

If I have extracted a plant with ethyl acetate and found that this extract has an effective activity, is it generally safe for humans to ingest this extracted plant material? Ethyl acetate is GRAS and would be evaporated away, so only trace amounts should remain in the extract.

Wondering if anyone has done this successfully? Thinking of backflushing and perhaps running methanol through the MilliQ filter column to clean it so it can be reused.

is it true that monoterpene alcohols are blue and esters are violet? what compounds do red green and yellow spots indicate?

If for instance the chosen best 3 solvents with respective solvent strengths are dichloromethane(3.1), ethyl acetate(4.4), chloroform(4.1) how to calculate how much hexane to add to adjust all polarities to the 3.1 strength of dichloromethane?

I want to irreversibly bind my small organic terpenoid compound to a bead so I can use it as in immunoprecipitation to fish out of a cell lysate what it might bind to. Does anyone have a procedure to attach small organic compound to a bead? I have different chromatography beads available such as silica, C18, MCI and some others that I could use or if you know of others I would like to know

Thanks!

I used a non-pathogenic fast growing Mycobacteria to screen my natural product compounds for activity. Now I want to test the active compounds in a disk diffusion assay against pathogenic Mycobacterium tuberculosis which grows very slowly, is this still possible to do? Has anyone done this with good results? I am at a university lab that has no funding so I cannot do any anti-Mtb assays that require buying anything, please let me know your suggestions. Thanks!