Saša Orlović

University of Novi Sad

The pedunculate oak (Quercus robur L.) is economically and ecologically one of the most significant tree species in Serbia, however, little is known about the influence of the riverbed distance and its water supply on ecophysiological responses of this species. Given the limited information on the light-response curve of photosynthesis for oaks in...

The loss of soil organic carbon stock and increased CO2 emission from soil are induced by various human activities. The aim of this study was to examine whether an anthropogenic influence during the regeneration of a pedunculate oak ( Quercus robur L.) stand can affect the increment of CO2 emission from the soil. The research was carried out within...

Bryophytes play important role in forest ecosystem functioning and their distribution and diversity are driven by numerous environmental factors. The aim of the present study was to bring new insights in deeper understanding of terrestrial bryophytes diversity in temperate forests, as well as to determine the environmental factors which have predom...

Simple Summary The genus Heterogynis, with its wingless females and males with uniformly dull-coloured wings that show the same translucent grey–black colour, is certainly not the most exciting group of lepidopterans. However, this genus is interesting due to its relatively high divergence of DNA barcode. This high molecular divergence indicated th...

Riparian forests are particularly vulnerable to environmental change and anthropogenic influences because they are highly dynamic ecosystems, thus proper adaptation measures are crucial. The implementation of these measures, however, strongly depends on the actors’ perceptions of the specific problems occurring in such forests. For understanding th...

Wild cherry ( Prunus avium L.) is a multi-purpose tree species with great ecological and economic importance for European forestry. Evaluating this species phenotypic diversity and quantitative traits characterization is of great importance to define its genetic resources conservation and breeding strategies. In this work, variations of physiologic...

As part of efforts for protection and restoration of variability of Juglans regia in Đerdap gorge, Serbia, where it grows as autochthonous relict species, the variability of ten measured and nine derived leaf morphometric parameters of half-sib progenies Persian walnut originating from Đerdap gorge was studied in this work. According to the contrib...

This paper presents analysis of anatomical parameters of leaves in Juglans regia half-sib lines from natural stands in Đerdap Gorge in Serbia. Thirty-five half-sib lines were examined, designated according to the number of their mother trees from R1-R35. Twenty measured and derived anatomical parameters were examined, describing stomatal and leaf b...

The Mura-Drava-Danube UNESCO Biosphere Reserve represents one of the best-preserved wetlands in the Danube River basin area. It is among the largest riverine protected areas in Europe; hence it was named the "Amazon of Europe". Recently, in the Interreg Europe project “Resilient riparian forests as ecological corridors in the Mura-Drava-Danube Bios...

The physiological and biochemical responses of pedunculate oaks (Quercus robur L.) to heat stress (HS) and mycorrhization (individually as well in combination) were estimated. One-year-old Q. robur seedlings were grown under controlled conditions in a pot experiment, inoculated with a commercial inoculum of ectomycorrhizal (ECM) fungi, and subjecte...

Increasing population, expansion of urban areas and rapid urbanization are the main causes of losing green spaces, which results in many environmental problems and threatens the quality of urban life. Urban green spaces are of great importance and urban planning issues nowadays gain increasing popularity. It is realized that biological methodologie...

We analyzed macro-(Ca, Mg, and K) and microelements (Cd, Cr, Cu, Fe, Mn, Ni, Pb, and Zn) in soil from three depths that correspond to different soil horizons (0-60, 61-75, and 76-160 cm) from three sites in Futoški park (Novi Sad, Serbia), which was measured by using the atomic absorption spectrophotometry (AAS). In this study, we tested the influe...

Finansijer: Ministarstvo poljoprivrede, šumarstva i vodoprivrede Republike Srbije- Uprava za šume (Funding: Ministry of agriculture, forestry and water management of the Republic of Serbia- Forestry Department), Rukovodilac: prof. dr Saša Orlović, Institut za nizijsko šumarstvo i životnu sredinu (Project leader: prof. dr Saša Orlović, Institute of...

Besides anthropogenic factors, climate change causes altered precipitation patterns that indirectly affect the increase of heavy metals in soils due to hydrological effects and enhanced leaching (i.e., Cd and Ni), especially in the vicinity of mines and smelters. Phytoextraction is a well-known, powerful “green” technique for environmental clean-up...

Sunoko" is a Sugar Refining Mill located in Bač that processes sugar beet and produces sugar. Around the factory there is a buffer zone that protects the nearby agricultural land and the DTD canal from pollutants. Field observation provided data on the current state and effectiveness of the buffer zone and the adaptability of existing tree and shru...

Finansijer: Ministarstvo poljoprivrede, šumarstva i vodoprivrede Republike Srbije- Uprava za šume (Funding: Ministry of agriculture, forestry and water management of the Republic of Serbia- Forestry Department), Rukovodilac: prof. dr Saša Orlović, Institut za nizijsko šumarstvo i životnu sredinu (Project leader: prof. dr Saša Orlović, Institute of...

In the first fifth of the 21st century, Novi Sad marked an intensive urban development that continues. This means that zones of single-family housing eventually become multi-family housing zones so that single-family houses, with backyards, have been replaced with multi-story buildings with yards that most often serve as parking spaces. With such d...

Environmental pollution is one of the biggest problems in the world today. The contaminated soil can be a secondary source of pollution to other parts of the ecosystem-water, air, and plants grown on contaminated soil. Phytoremediation, the use of plants to extract, sequester and detoxify pollutants is a new and powerful technique for environmental...

Figure S2. Phylogenetic tree generated from a maximum likelihood (ML) analysis based on ITS sequence data showing the position of Scleroderma citrinum in relation to its closely related Scleroderma species. ML bootstrap support values greater than 70% are indicated above or below branches. The specimen sequenced in this study is shown in bold. Suil...

Figure S3. Phylogenetic tree generated from a maximum likelihood analysis (ML) based on ITS sequence data of the Quercus robur powdery mildews showing the position of Erysiphe alphitoides in relation to closely related Erysiphe quercicola. ML bootstrap support values greater than 70% are indicated on branches. The type specimens are marked with an...

In light of climate change, pedunculate oak (Q. robur L.) was marked as the most threatened European tree species. Pedunculate oak is particularly jeopardized by powdery mildew disease caused by Erysiphe alphitoides. We hypothesized that priming of this tree species with ectomycorrhizal fungi could mitigate biotic stress and produce bioprotective p...

U okviru Projekta „Zaštita jedinstvenog biodiverziteta životne sredine doline Drine“, Prekogranični program Srbija - Bosna i Hercegovina u okviru Instrumenta pretpristupne pomoći (IPA II) Evropske komisije od 2014. do 2020. godine realizovan je veći broj istraživanja čiji rezultati su prikazani u nekoliko studija: „Assesment study Bosnia, Molecular...

Tree-ring width (TRW), stable carbon isotope ratio (δ¹³C) and intrinsic water use efficiency (iWUE) data set chronologies were built for the period 1961–2000 for two oak species (pedunculate oak – Quercus robur L. and Turkey oak – Quercus cerris L.) in northwestern Serbia (Vojvodina province). We focused on the response of the two oak species to me...

The effects of drought simulated via osmotic stress induced by polyethylene glycol (PEG) in the growing medium were examined on two Wild cherry (Prunus avium L.) clones (6A and 8A) based on thirteen morphometric, physiological , and biochemical traits. The shoot tips were exposed to two PEG concentrations (20 and 50 g L-1) in growing medium designe...

Although oaks belong to the economically most important hardwood tree species in Europe, data on the diversity of ectomycorrhizal (ECM) fungi on pedunculate oak in the Republic of Serbia are deficient. The aim of our study was to give the first insight into the diversity of ECM fungi in the young stand of pedunculate oak in Morović, Serbia. The com...

The general aim of this work was to compare the leaf-level responses of different protective components to water deficit and high temperatures in Quercus cerris L. and Quercus robur L. Several biochemical components of the osmotic adjustment and antioxidant system were investigated together with changes in hormones. Q. cerris and Q. robur seedlings...

Urban parks, as one of the most significant urban green areas (UGA), bring many direct or indirect benefits, including but not limited to ecosystem services (ES) (Mexia et al. 2018). These services are often generalized, unquantified and poorly supported by empirical evidence and their negative consequences-disservices are rarely mentioned (Roman e...

Clopyralid and imazamox are successfully used for weed control during the first years of regeneration of pedunculate oak forests. Hence, the question that arises is how these herbicides affect microorganisms, especially the activity of dehydrogenase enzyme, when they reach the soil. Two study sites were selected in regenerated pedunculate oak fores...

Oak powdery mildew (Erysiphe alphitoides (Griffon and Maubl.) U. Braun and S. Takam.)) is one of the most common foliar pathogenic organism of oaks, exploiting harmful effects, particularly on young seedlings. To assess and evaluate these negative effects, an experiment was conducted under semi-controlled conditions with 20 one-year-old seedlings o...

Numerous medical studies have shown the positive effects of forests on different aspects of human health. This study deals with the content of major terpenes in dominant coniferous species in Tara National Park, Serbia, in order to explore the potential for the development of a novel health tourism programme based on forest therapy. Main terpenes w...

In a changing world, riparian forests are coming under more and more stress from biotic threats. This impacts all stages from saplings to older trees. One of the most dominant biotic threats are alien species and eruptive pest species. Sustainable silvicultural methods are required to reduce the risk of such biotic threats. We investigated the infl...

Urban parks provide many ecosystem services, and conserving biodiversity is one of the most important among them. Keeping track of biodiversity indices and their evolution over time is a useful procedure for preserving biodiversity within urban habitats. The paper presents an application of the R programming language and the R package “vegan” for c...

Wild cherry (Prunus avium L.) is fast-growing noble tree species highly valued for its economic and environmental importance, while conservation of genetic variability of this species is considered to be an important part of the preservation of biodiversity, in general. In this work, we studied the difference in survival and growth of in vitro root...

Key message Leaf shape in European beech provenances varies geographically, with narrower and longer lamina observed in southern provenances, indicating a direct selection favoring leaf shape that likely safeguards trees performances under less favorable growing conditions. AbstractSpatial and environmental patterns of European beech (Fagus sylvati...

European beech is one of the most important and most common tree species in Europe, which have extremely wide amplitude in sense of horizontal and vertical distribution. In Serbia and Bosnia and Herzegovina European beech (Fagus sylvatica L.) represents one of the most important tree species from an economic and ecological point of view, because it...

Although sessile oak is one of the most important deciduous forest tree species in Europe, data on the diversity of ectomycorrhizal (ECM) fungi on sessile oaks in the Republic of Serbia are scarce. The aim of this study was to provide the first insight into the diversity of ECM fungi on sessile oak in Serbia. Two sites Info center and Brankovac, lo...

Pinus sylvestris bark represents a rich source of active compounds with antifungal, antibacterial, and antioxidant properties. The current study aimed to evaluate the antifungal potential of P. sylvestris bark against Botryosphaeria dothidea, Dothiorella sarmentorum, and Neofusicoccum parvum (Botryosphaeriaceae) through its chemical (water extracts...

Extreme weather conditions, namely droughts, heat waves, heavy rains, floods, and landslides are becoming more frequent globally and in Serbia as a result of climate change. Generally, various parts of human society are affected by changing climate conditions. Forest ecosystems are one of the most sensitive systems to weather and climate. In that s...

Figure S2. Phylogenetic tree generated from a maximum likelihood analysis (ML) based on a concatenated alignment of tef 1-α and RPB2 sequence data showing the position of Trichoderma citrinoviride in relation to its closely related species belonging to the Longibranchiatum clade. ML and maximum parsimony (MP) bootstrap support values greater than 7...

Table S1. Sequences used in the phylogenetic analyses.

Table S2. Nucleotide differences between Trichoderma sp. and its closest phylogenetic relative T. citrinoviride.

Figure S3. Effects of VOCs of Trichoderma citrinoviride DEMf TR4 on mycelial growth of Dothiorella sarmentorum (a) and Botryosphaeria dothidea (b)

Figure S1. Phylogenetic trees generated from Maximum likelihood (ML) analyses based on a single gene alignment of a) RPB2, b) TEF 1-α, and c) ITS sequence data. ML bootstrap support values greater than 70% are indicated at the tree nodes (ML). The type strains are marked with an asterisk. Isolates sequenced in this study are given in bold and highl...

Associations of pedunculate oak (Quercus robur L.) radial growth with satellite-based soil moisture (SM) during the intensive tree growth period over a 30-year time span (1980–2010) were analyzed. This study included tree-ring width (TRW) chronologies from 22 stands located in four southeastern (SE) European countries (Slovenia, Croatia, Serbia and...

Urban Green Spaces (UGS) are providing multiple health related ecosystem services (ES) to Public Health by improving quality of life (QoL) and well-being. The aim of research is to provide an insight of UGS in Novi Sad, and its potential to support public health, using publicly available data. Novi Sad, as the capital of Vojvodina Province (Republi...

The prioritization of alien species according to the magnitude of their environmental impacts has become increasingly important for the management of invasive alien species. In this study, we applied the Environmental Impact Classification of Alien Taxa (EICAT) to classify alien taxa from three different taxonomic groups to facilitate the prioritis...

The survival of forest tree species within certain areas of their distributions is in question due to the increasing occurrence of disturbances and degradation processes in forest ecosystems due to climate change. The aim of this paper is to predict changes in the spatial distribution of the ten most important tree species in Serbia (European beec...

Populus × euramericana cl. “I-214” is the most widely grown poplar clone in Serbia. In summer of 2020, cankers were observed on stems and branches of 1-year-old P. × euramericana cl. “I-214” plants in two poplar nurseries in Vojvodina, Serbia. The cankers were sometimes accompanied by white, foamy odorous exudates that flowed from the canker sites...

Poplars (Populus spp.) are widespread, economically, and ecologically important tree species in Serbia. Because of their rapid growth, and high-quality wood, poplar clones are planted over huge areas to produce wood, and for multiple industrial uses, i.e., peeled veneers, and pellets. During 2019 and 2020, cankers were observed on the trunk and bra...

New Method Crispr/Cas9 Poplar biotic stress improvement

Understanding intra-specific variation in leaf functional traits is one of the key requirements for the evaluation of species adaptive capacity to ongoing climate change, as well as for designing long-term breeding and conservation strategies. Hence, data of 19 functional traits describing plant physiology, antioxidant properties, anatomy and morph...

Understanding intraspecific genetic variation is one of the principal requirements for the evaluation of tree species capacity to cope with intensive climatic changes, as well as designing long-term conservation programs. Herein, we evaluated the genetic diversity and genetic structure of seven pedunculate oak (Quercus robur L.) populations, locate...

Plant community biomass production is co‐dependent on climatic and edaphic factors that are often covarying and non‐independent. Disentangling how these factors act in isolation is challenging, especially along large climatic gradients that can mask soil effects. As anthropogenic pressure increasingly alters local climate and soil resource supply u...

This paper presents an analysis of the radial growth, tree dimensions, and allometry of three phenological pedunculate oak (Quercus robur L.; QURO) varieties (early (E-QURO), typical (T-QURO), and late (L-QURO)), from a common garden experiment. We focused on the resistance and resilience of each variety to drought events, which occurred in 2012 an...

Multiple positive effects that forests have on human health and overall well-being have been reported widely in the literature. Still, multiple elements of this relationship remain unidentified and unexplained. In this study, the composition of leaf volatile organic compounds (BVOCs) content in three common coniferous species: the Austrian pine (Pi...

The ongoing climate change have multi-faceted effects not only on metabolism of plants, but also on the soil properties and mycorrhizal fungal community. Under climate change the stability of the entire forest ecosystems and the carbon balance depend to a large degree on the interactions between trees and mycorrhizal fungi. The main drivers of clim...

Table S2. PCR conditions used in this study.

Phylogenetic trees generated from Bayesian interference analyses based on a single gene alignment of ITS, tef 1-alpha and TUB2 sequences data showing the relationships of Botryosphaeria dothidea and Neofusicoccum yunannense with closely related species. ML bootstrap support values greater than 70% and Bayesian posterior probability values (PP) grea...

Sequoiadendron giganteum Lindl. [Buchholz] is a long-lived tree species endemic to the Sierra Nevada Mountains in California. Due to its massive size and beauty, S. giganteum is a popular ornamental tree planted in many parts of the world, including Europe. Since 2017, scattered branch die-back has been observed on S. giganteum trees in Zagreb, Cro...